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The map label for this gene is eno [H]

Identifier: 169627416

GI number: 169627416

Start: 315003

End: 316277

Strand: Reverse

Name: eno [H]

Synonym: MAB_0312c

Alternate gene names: 169627416

Gene position: 316277-315003 (Counterclockwise)

Preceding gene: 169627417

Following gene: 169627415

Centisome position: 6.24

GC content: 66.75

Gene sequence:

>1275_bases
ATGACCGGAACACGAATCAGCAGAGTCCACGCCCGACGAGTATGGGACTCCCGGGGGCGTCCGACCATCGAGGCGGAGTT
GCTGCTGAGCGACGGCAGTACCGGGCGCGCAATCGCCCCGGCAGGCGCGTCCCGCGGCAGCGGTGAGGTTACCGATTTAC
GGGACGGCGGCGGGCCTTTCGCGGGGTACGACGTGACAGCGGCCGTGGCCGCGGCCCGCGGGCTGGCGCACCATCTGGTA
GACGTCGACGCCGCGAATCAGCAGGCCGTGGATGCGGCGCTGACGGCTGCCGACCAATCGGCCGAGTTCGCAGCCATCGG
CGGCAATACCGCGGTGGCGATGTCCATGGCGGCCGCCCACGCCGCGGCCGCATCACACGGAATCCCGTTGTGGCGGTATC
TGATCGGTGACAAACCGGCCGCGATGCCGCTGCCCGAGATCCAAGTGATCGGTGGGGGCGCGCACGCGGCGCGCCGGACC
GACGTCCAGGACTTCATGGTGGTACCCGCTGCGGCGAACACGATAGAAGAAGGCCTCAACTGGCTAGCGGAGATTCACCG
CGCCTGCGGAGCAATACTTTCCGACCGAGGCAGACTCGCCGGCGTTGCCGATGAGGGTGGCTGGTGGCCGATGGTGGACT
CCAACGAGGATGCCCTGGAGCTACTGGTGGCCTCCATCGAACGCGCTGGTTTGCAGCCGGGGACCCAGGCCACAATCGCC
CTGGATATCGCGTCGACTGAGTTCGGTAGCGGCGGCCGCTACCGCCTGGGGCTCGATGGCACCGAGCTCGACTCCGACGC
ATGGTCGGAGGTTCTGATCGGTTGGATCGATAAGTACCCGATCGCCTCCATCGAGGACCCGCTAGCCGAGGATGACCCGG
CAGCGCTGGCAGCCTTCACTCAAGCAGTGGGACATCGTGTCCTGGTTGTCGGAGACGATTTCACGGTGACCGACGCCGGC
CGGGTGCGGCGAGCAACCGAGTCCGGCGCATGCAACGGCTTACTGGTAAAGCCGAATCAGGCGGGCACGCTCAGTGCCGC
CAAGACCGCACACGACACCGCTCACGACTGCGGATGGGACACCATCGTGTCCGCGCGATCCGGCGAAAGCGAGGATGTCA
CCATCGCGCACCTGGCGGTCGGATGGGGAGCCGAGCAACTCAAGGTGGGCTCCATCACCCGATCCGAACGCACCGCCAAG
TGGAACGAGCTGCTCCGCATCGACGAACAGCTTGGCGGAGCACCGATTCGGCTAGGACGATTGTCCCTCGGCTAG

Upstream 100 bases:

>100_bases
TGTCTACCGCGCAACGTGAATCATTGCGTACACGGGCCGGGAGGGCGCTACACGAATCGGCCACGGACCCCTTGGCCGTC
GCACAACAGTTGGAAGGGCT

Downstream 100 bases:

>100_bases
GCACGTTTTGGTCGCCTAATCGAGATCTCGGGTGCGGACCGCCCCGAAGCGGGCTAGCGTAGAAACTGTGCCTATCGCAT
CGGGATCGGCGCACGCCGAG

Product: enolase (Eno)

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTGTRISRVHARRVWDSRGRPTIEAELLLSDGSTGRAIAPAGASRGSGEVTDLRDGGGPFAGYDVTAAVAAARGLAHHLV
DVDAANQQAVDAALTAADQSAEFAAIGGNTAVAMSMAAAHAAAASHGIPLWRYLIGDKPAAMPLPEIQVIGGGAHAARRT
DVQDFMVVPAAANTIEEGLNWLAEIHRACGAILSDRGRLAGVADEGGWWPMVDSNEDALELLVASIERAGLQPGTQATIA
LDIASTEFGSGGRYRLGLDGTELDSDAWSEVLIGWIDKYPIASIEDPLAEDDPAALAAFTQAVGHRVLVVGDDFTVTDAG
RVRRATESGACNGLLVKPNQAGTLSAAKTAHDTAHDCGWDTIVSARSGESEDVTIAHLAVGWGAEQLKVGSITRSERTAK
WNELLRIDEQLGGAPIRLGRLSLG

Sequences:

>Translated_424_residues
MTGTRISRVHARRVWDSRGRPTIEAELLLSDGSTGRAIAPAGASRGSGEVTDLRDGGGPFAGYDVTAAVAAARGLAHHLV
DVDAANQQAVDAALTAADQSAEFAAIGGNTAVAMSMAAAHAAAASHGIPLWRYLIGDKPAAMPLPEIQVIGGGAHAARRT
DVQDFMVVPAAANTIEEGLNWLAEIHRACGAILSDRGRLAGVADEGGWWPMVDSNEDALELLVASIERAGLQPGTQATIA
LDIASTEFGSGGRYRLGLDGTELDSDAWSEVLIGWIDKYPIASIEDPLAEDDPAALAAFTQAVGHRVLVVGDDFTVTDAG
RVRRATESGACNGLLVKPNQAGTLSAAKTAHDTAHDCGWDTIVSARSGESEDVTIAHLAVGWGAEQLKVGSITRSERTAK
WNELLRIDEQLGGAPIRLGRLSLG
>Mature_423_residues
TGTRISRVHARRVWDSRGRPTIEAELLLSDGSTGRAIAPAGASRGSGEVTDLRDGGGPFAGYDVTAAVAAARGLAHHLVD
VDAANQQAVDAALTAADQSAEFAAIGGNTAVAMSMAAAHAAAASHGIPLWRYLIGDKPAAMPLPEIQVIGGGAHAARRTD
VQDFMVVPAAANTIEEGLNWLAEIHRACGAILSDRGRLAGVADEGGWWPMVDSNEDALELLVASIERAGLQPGTQATIAL
DIASTEFGSGGRYRLGLDGTELDSDAWSEVLIGWIDKYPIASIEDPLAEDDPAALAAFTQAVGHRVLVVGDDFTVTDAGR
VRRATESGACNGLLVKPNQAGTLSAAKTAHDTAHDCGWDTIVSARSGESEDVTIAHLAVGWGAEQLKVGSITRSERTAKW
NELLRIDEQLGGAPIRLGRLSLG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI4503571, Length=429, Percent_Identity=36.3636363636364, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI301897477, Length=429, Percent_Identity=37.2960372960373, Blast_Score=266, Evalue=4e-71,
Organism=Homo sapiens, GI301897469, Length=429, Percent_Identity=37.2960372960373, Blast_Score=266, Evalue=4e-71,
Organism=Homo sapiens, GI5803011, Length=421, Percent_Identity=36.8171021377672, Blast_Score=258, Evalue=8e-69,
Organism=Homo sapiens, GI301897479, Length=426, Percent_Identity=35.6807511737089, Blast_Score=245, Evalue=5e-65,
Organism=Homo sapiens, GI169201331, Length=343, Percent_Identity=24.4897959183673, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI169201757, Length=343, Percent_Identity=24.4897959183673, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI239744207, Length=343, Percent_Identity=24.4897959183673, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1789141, Length=416, Percent_Identity=39.4230769230769, Blast_Score=288, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI71995829, Length=437, Percent_Identity=35.9267734553776, Blast_Score=260, Evalue=8e-70,
Organism=Caenorhabditis elegans, GI17536383, Length=430, Percent_Identity=36.046511627907, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI32563855, Length=191, Percent_Identity=38.7434554973822, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=37.962962962963, Blast_Score=265, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=37.7314814814815, Blast_Score=263, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=37.7314814814815, Blast_Score=263, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6321693, Length=424, Percent_Identity=38.9150943396226, Blast_Score=254, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6321968, Length=435, Percent_Identity=38.3908045977011, Blast_Score=247, Evalue=2e-66,
Organism=Drosophila melanogaster, GI281360527, Length=422, Percent_Identity=35.781990521327, Blast_Score=244, Evalue=8e-65,
Organism=Drosophila melanogaster, GI17137654, Length=422, Percent_Identity=35.781990521327, Blast_Score=244, Evalue=8e-65,
Organism=Drosophila melanogaster, GI24580918, Length=422, Percent_Identity=35.781990521327, Blast_Score=244, Evalue=9e-65,
Organism=Drosophila melanogaster, GI24580916, Length=422, Percent_Identity=35.781990521327, Blast_Score=244, Evalue=9e-65,
Organism=Drosophila melanogaster, GI24580920, Length=422, Percent_Identity=35.781990521327, Blast_Score=244, Evalue=9e-65,
Organism=Drosophila melanogaster, GI24580914, Length=422, Percent_Identity=35.781990521327, Blast_Score=244, Evalue=9e-65,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 44078; Mature: 43946

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGTRISRVHARRVWDSRGRPTIEAELLLSDGSTGRAIAPAGASRGSGEVTDLRDGGGPF
CCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEECCCCCCCCCCCEEECCCCCCCC
AGYDVTAAVAAARGLAHHLVDVDAANQQAVDAALTAADQSAEFAAIGGNTAVAMSMAAAH
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHH
AAAASHGIPLWRYLIGDKPAAMPLPEIQVIGGGAHAARRTDVQDFMVVPAAANTIEEGLN
HHHHHCCCHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHCCCHHEEEECCHHHHHHHHHH
WLAEIHRACGAILSDRGRLAGVADEGGWWPMVDSNEDALELLVASIERAGLQPGTQATIA
HHHHHHHHHHHHHCCCCCEEEECCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEE
LDIASTEFGSGGRYRLGLDGTELDSDAWSEVLIGWIDKYPIASIEDPLAEDDPAALAAFT
EEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
QAVGHRVLVVGDDFTVTDAGRVRRATESGACNGLLVKPNQAGTLSAAKTAHDTAHDCGWD
HHHCCEEEEEECCEEECCCHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHCCCHHHCCCH
TIVSARSGESEDVTIAHLAVGWGAEQLKVGSITRSERTAKWNELLRIDEQLGGAPIRLGR
HEEECCCCCCCCEEEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
LSLG
ECCC
>Mature Secondary Structure 
TGTRISRVHARRVWDSRGRPTIEAELLLSDGSTGRAIAPAGASRGSGEVTDLRDGGGPF
CCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEECCCCCCCCCCCEEECCCCCCCC
AGYDVTAAVAAARGLAHHLVDVDAANQQAVDAALTAADQSAEFAAIGGNTAVAMSMAAAH
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHH
AAAASHGIPLWRYLIGDKPAAMPLPEIQVIGGGAHAARRTDVQDFMVVPAAANTIEEGLN
HHHHHCCCHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHCCCHHEEEECCHHHHHHHHHH
WLAEIHRACGAILSDRGRLAGVADEGGWWPMVDSNEDALELLVASIERAGLQPGTQATIA
HHHHHHHHHHHHHCCCCCEEEECCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEE
LDIASTEFGSGGRYRLGLDGTELDSDAWSEVLIGWIDKYPIASIEDPLAEDDPAALAAFT
EEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
QAVGHRVLVVGDDFTVTDAGRVRRATESGACNGLLVKPNQAGTLSAAKTAHDTAHDCGWD
HHHCCEEEEEECCEEECCCHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHCCCHHHCCCH
TIVSARSGESEDVTIAHLAVGWGAEQLKVGSITRSERTAKWNELLRIDEQLGGAPIRLGR
HEEECCCCCCCCEEEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
LSLG
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA