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The map label for this gene is phzA [H]

Identifier: 169627401

GI number: 169627401

Start: 298915

End: 299550

Strand: Direct

Name: phzA [H]

Synonym: MAB_0297

Alternate gene names: 169627401

Gene position: 298915-299550 (Clockwise)

Preceding gene: 169627400

Following gene: 169627402

Centisome position: 5.9

GC content: 62.26

Gene sequence:

>636_bases
ATGACGACCGCCACTCGCGGTATTCCCATGATCGCCGAATATGACTTGCCGACAGTCGACCTGCTGCCGTCTAACACGGC
AACCTGGCGGATCGACCCCGACCGGGCCGTGCTGCTCATTCACGATATGCAGCGCTATTTTCTGGAGCCACTTCCGGTTT
CCTTGCGCCAGGCATTGGTGCGTAACGTCGCCGCCATCCGTGATCGCAGCGTGGCGGCAGGTATCCCGATCGCCTACACA
GCGCAACCCGGCAGCATGACCCAGGAACAGCGGGGCCTGCTCAAGGATTTTTGGGGCCCGGGGATGCGCGTTGACGAGGA
GCACCGTCAGATCGTCGATCCCATCGCTCCGGACGCGGGTGACTGGGTGTTCACCAAGTGGCGCTACAGCGCATTCCACC
GCTCGGCGCTGCTGGAGCGCATGCATGCTGCCGGGCGCGACCAGTTGGTGATCTGTGGCGTCTACGCACATGTCGGTGTG
CTGATGACGGCGGTAGACGCGTTCACCCACGATATCGAGACCTTCTTGGTCGCCGACGCGATCGCGGACTTTGACGACGA
ATCACATTGGATGGCATTGGAGTACGCAGCACGGCGGTGTGCCGTGCTGACCGTTGCCGGAGAGGTGCTGACATGA

Upstream 100 bases:

>100_bases
CATCGCCGATGCGGTGGCCTTCCTGCTCTCCGACCAAGCCGCTCACATCACCATGCACACGCTGGTGGTCGACGGTGGCG
CGACACTAGGAGTCTGAACA

Downstream 100 bases:

>100_bases
GCGATCTGCTGGGGCGCGTGCTGGCCGGAGGCGCCGGACCGTTCGCGCTGCTCTACCGGCCGGAATCCGGTGCGCCCGAC
ACCGTCGACGTCGTGAGCGG

Product: isochorismatase (phenazine biosynthesis) PhzD

Products: NA

Alternate protein names: 2,3 dihydro-2,3 dihydroxybenzoate synthase [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTTATRGIPMIAEYDLPTVDLLPSNTATWRIDPDRAVLLIHDMQRYFLEPLPVSLRQALVRNVAAIRDRSVAAGIPIAYT
AQPGSMTQEQRGLLKDFWGPGMRVDEEHRQIVDPIAPDAGDWVFTKWRYSAFHRSALLERMHAAGRDQLVICGVYAHVGV
LMTAVDAFTHDIETFLVADAIADFDDESHWMALEYAARRCAVLTVAGEVLT

Sequences:

>Translated_211_residues
MTTATRGIPMIAEYDLPTVDLLPSNTATWRIDPDRAVLLIHDMQRYFLEPLPVSLRQALVRNVAAIRDRSVAAGIPIAYT
AQPGSMTQEQRGLLKDFWGPGMRVDEEHRQIVDPIAPDAGDWVFTKWRYSAFHRSALLERMHAAGRDQLVICGVYAHVGV
LMTAVDAFTHDIETFLVADAIADFDDESHWMALEYAARRCAVLTVAGEVLT
>Mature_210_residues
TTATRGIPMIAEYDLPTVDLLPSNTATWRIDPDRAVLLIHDMQRYFLEPLPVSLRQALVRNVAAIRDRSVAAGIPIAYTA
QPGSMTQEQRGLLKDFWGPGMRVDEEHRQIVDPIAPDAGDWVFTKWRYSAFHRSALLERMHAAGRDQLVICGVYAHVGVL
MTAVDAFTHDIETFLVADAIADFDDESHWMALEYAARRCAVLTVAGEVLT

Specific function: Involved in the biosynthesis of the antibiotic phenazine, a nitrogen-containing heterocyclic molecule having important roles in virulence, competition and biological control. This isochorismatase may remove pyruvate from chorismate during the formation of

COG id: COG1535

COG function: function code Q; Isochorismate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family [H]

Homologues:

Organism=Escherichia coli, GI1786811, Length=208, Percent_Identity=46.1538461538462, Blast_Score=196, Evalue=7e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016291
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.3.2.1 [H]

Molecular weight: Translated: 23577; Mature: 23446

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTATRGIPMIAEYDLPTVDLLPSNTATWRIDPDRAVLLIHDMQRYFLEPLPVSLRQALV
CCCCCCCCCEEEECCCCEEEECCCCCEEEEECCCCEEEEEEHHHHHHCCCCCHHHHHHHH
RNVAAIRDRSVAAGIPIAYTAQPGSMTQEQRGLLKDFWGPGMRVDEEHRQIVDPIAPDAG
HHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCC
DWVFTKWRYSAFHRSALLERMHAAGRDQLVICGVYAHVGVLMTAVDAFTHDIETFLVADA
CEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
IADFDDESHWMALEYAARRCAVLTVAGEVLT
HHCCCCCCCEEHHHHHHHHHEEEEECCHHCC
>Mature Secondary Structure 
TTATRGIPMIAEYDLPTVDLLPSNTATWRIDPDRAVLLIHDMQRYFLEPLPVSLRQALV
CCCCCCCCEEEECCCCEEEECCCCCEEEEECCCCEEEEEEHHHHHHCCCCCHHHHHHHH
RNVAAIRDRSVAAGIPIAYTAQPGSMTQEQRGLLKDFWGPGMRVDEEHRQIVDPIAPDAG
HHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCC
DWVFTKWRYSAFHRSALLERMHAAGRDQLVICGVYAHVGVLMTAVDAFTHDIETFLVADA
CEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
IADFDDESHWMALEYAARRCAVLTVAGEVLT
HHCCCCCCCEEHHHHHHHHHEEEEECCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8586283 [H]