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The map label for this gene is tgt [H]

Identifier: 169627380

GI number: 169627380

Start: 273306

End: 274526

Strand: Direct

Name: tgt [H]

Synonym: MAB_0275

Alternate gene names: 169627380

Gene position: 273306-274526 (Clockwise)

Preceding gene: 169627377

Following gene: 169627381

Centisome position: 5.39

GC content: 65.36

Gene sequence:

>1221_bases
ATGACTGACCCGTATTTCTCCATTGACGCCACCTTGTCCGGAACCGCGGGCCGGGCCGGAGTCATCCACACCCCGCACGG
CGACATCCAGACCCCGGCGTTCATCGCGGTCGGCACCAAGGCCACCGTCAAGGCGGTGCTGCCGGAAACCATGGCCGCAC
TCGGCGCCCAGGCGGTGCTCGCCAACGCCTACCACCTGTATCTGCAACCGGGCCCCGACATCGTCGACGAGGCCGGCGGA
CTGGGTGCCTTCATGAACTGGCCCGGGCCGACGTTCACCGACAGTGGCGGCTTCCAGGTGCTCTCGCTGGGCTCCGGGGT
GCGCAAGGTGATGGCGATGGACGTCAACAGAGCCAGGGCCGACGATGTCACGGTCGCCGGTAAGGACAAGTTGGCTCATG
TCGACGATGACGGCGTCACCTTCACCTCACATCTGGACGGCTCTGCGCACCGGTTCACCCCGGAGGTGTCCATGCAGATT
CAGCACCAGCTCGGTGCCGACATCATGTTCGCCTTCGACGAGCTCACCACGCTGATCAACACCCGTGAGTATCAAGAGGA
TTCGGTGCAACGCACCCACGAGTGGGCGCAGCGTTGCCTGGACGAGCACGAGAAGCTGACCCGGGAGCGCGCCGACAAGC
CGTATCAGGCGTTGTTCGGGGTGGTGCAGGGTGCGCAGTACGAGGACCTGCGGCGGCAGGCCGCGCGTGGGCTGGAATCT
CTTGCCGGACCGTCCGGACGGGGATTCGATGGCTACGGAATCGGTGGTGCGCTGGAGAAGCAGAATCTCGGCACGATCGT
CGGCTGGGTGACATCCGAACTGCCCGAGCACAAGCCGCGTCACCTGTTGGGGATCAGTGAGCCTGACGACCTGTTCGCGG
CGGTCGCGGCGGGCGCGGACACCTTCGACTGCGTGTCCCCGTCCCGAGTCGCGCGCAACGCCGCCGTCTACACCCGCGAC
GGGCGGGTGAACATCACCGGGGCCAGGTACCGCCGGGATTTCACCCCCATCGACGCCGAATGTGACTGCTACACCTGCGC
GCATTACACCCGGGCCTACATGCATCACCTGTTCAAGGCCAAGGAGATCCTTGCCTCGACTCTGGCCACCATCCACAACG
AGCGGTTCACCATCGGCTTGGTAGACCGCATTCGCGCCAGCATCGTGGAAGGCTGCTTCCAGGAGCTGCGCGAAGACACC
CTGGGCCGCTATTACCGCTGA

Upstream 100 bases:

>100_bases
GTGGTGGTCCCATGCTCCAGCGCGAAAAGATCAGACGTCGATGTCATGACCTGAGCCTACGACCCACGGCCCCGACGCGC
CCAGGCAAAATAACTCCCAA

Downstream 100 bases:

>100_bases
GCCACGAAGCTCAGGTGCATCTCAGTATTTCGGCGCACACTGGACACATGCCTATTACGACCGACCCGGGCACGCTGTTG
CTTCAGGTGCTCGACCCGGC

Product: queuine tRNA-ribosyltransferase (TGT)

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MTDPYFSIDATLSGTAGRAGVIHTPHGDIQTPAFIAVGTKATVKAVLPETMAALGAQAVLANAYHLYLQPGPDIVDEAGG
LGAFMNWPGPTFTDSGGFQVLSLGSGVRKVMAMDVNRARADDVTVAGKDKLAHVDDDGVTFTSHLDGSAHRFTPEVSMQI
QHQLGADIMFAFDELTTLINTREYQEDSVQRTHEWAQRCLDEHEKLTRERADKPYQALFGVVQGAQYEDLRRQAARGLES
LAGPSGRGFDGYGIGGALEKQNLGTIVGWVTSELPEHKPRHLLGISEPDDLFAAVAAGADTFDCVSPSRVARNAAVYTRD
GRVNITGARYRRDFTPIDAECDCYTCAHYTRAYMHHLFKAKEILASTLATIHNERFTIGLVDRIRASIVEGCFQELREDT
LGRYYR

Sequences:

>Translated_406_residues
MTDPYFSIDATLSGTAGRAGVIHTPHGDIQTPAFIAVGTKATVKAVLPETMAALGAQAVLANAYHLYLQPGPDIVDEAGG
LGAFMNWPGPTFTDSGGFQVLSLGSGVRKVMAMDVNRARADDVTVAGKDKLAHVDDDGVTFTSHLDGSAHRFTPEVSMQI
QHQLGADIMFAFDELTTLINTREYQEDSVQRTHEWAQRCLDEHEKLTRERADKPYQALFGVVQGAQYEDLRRQAARGLES
LAGPSGRGFDGYGIGGALEKQNLGTIVGWVTSELPEHKPRHLLGISEPDDLFAAVAAGADTFDCVSPSRVARNAAVYTRD
GRVNITGARYRRDFTPIDAECDCYTCAHYTRAYMHHLFKAKEILASTLATIHNERFTIGLVDRIRASIVEGCFQELREDT
LGRYYR
>Mature_405_residues
TDPYFSIDATLSGTAGRAGVIHTPHGDIQTPAFIAVGTKATVKAVLPETMAALGAQAVLANAYHLYLQPGPDIVDEAGGL
GAFMNWPGPTFTDSGGFQVLSLGSGVRKVMAMDVNRARADDVTVAGKDKLAHVDDDGVTFTSHLDGSAHRFTPEVSMQIQ
HQLGADIMFAFDELTTLINTREYQEDSVQRTHEWAQRCLDEHEKLTRERADKPYQALFGVVQGAQYEDLRRQAARGLESL
AGPSGRGFDGYGIGGALEKQNLGTIVGWVTSELPEHKPRHLLGISEPDDLFAAVAAGADTFDCVSPSRVARNAAVYTRDG
RVNITGARYRRDFTPIDAECDCYTCAHYTRAYMHHLFKAKEILASTLATIHNERFTIGLVDRIRASIVEGCFQELREDTL
GRYYR

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=396, Percent_Identity=36.8686868686869, Blast_Score=260, Evalue=1e-69,
Organism=Escherichia coli, GI1786607, Length=406, Percent_Identity=39.6551724137931, Blast_Score=284, Evalue=6e-78,
Organism=Caenorhabditis elegans, GI17544680, Length=389, Percent_Identity=34.4473007712082, Blast_Score=243, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI25146502, Length=214, Percent_Identity=26.1682242990654, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19920510, Length=401, Percent_Identity=35.1620947630923, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI21355311, Length=239, Percent_Identity=27.6150627615063, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 44411; Mature: 44280

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPYFSIDATLSGTAGRAGVIHTPHGDIQTPAFIAVGTKATVKAVLPETMAALGAQAVL
CCCCCEEEEEEECCCCCCCCEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
ANAYHLYLQPGPDIVDEAGGLGAFMNWPGPTFTDSGGFQVLSLGSGVRKVMAMDVNRARA
HCCEEEEECCCCCHHHCCCCCCEEECCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCC
DDVTVAGKDKLAHVDDDGVTFTSHLDGSAHRFTPEVSMQIQHQLGADIMFAFDELTTLIN
CCEEEECCCCEEEECCCCCEEEECCCCCCHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
TREYQEDSVQRTHEWAQRCLDEHEKLTRERADKPYQALFGVVQGAQYEDLRRQAARGLES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
LAGPSGRGFDGYGIGGALEKQNLGTIVGWVTSELPEHKPRHLLGISEPDDLFAAVAAGAD
HCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHCCCC
TFDCVSPSRVARNAAVYTRDGRVNITGARYRRDFTPIDAECDCYTCAHYTRAYMHHLFKA
CCCCCCHHHHHCCCEEEEECCEEEEEHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KEILASTLATIHNERFTIGLVDRIRASIVEGCFQELREDTLGRYYR
HHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TDPYFSIDATLSGTAGRAGVIHTPHGDIQTPAFIAVGTKATVKAVLPETMAALGAQAVL
CCCCEEEEEEECCCCCCCCEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
ANAYHLYLQPGPDIVDEAGGLGAFMNWPGPTFTDSGGFQVLSLGSGVRKVMAMDVNRARA
HCCEEEEECCCCCHHHCCCCCCEEECCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCC
DDVTVAGKDKLAHVDDDGVTFTSHLDGSAHRFTPEVSMQIQHQLGADIMFAFDELTTLIN
CCEEEECCCCEEEECCCCCEEEECCCCCCHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHH
TREYQEDSVQRTHEWAQRCLDEHEKLTRERADKPYQALFGVVQGAQYEDLRRQAARGLES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
LAGPSGRGFDGYGIGGALEKQNLGTIVGWVTSELPEHKPRHLLGISEPDDLFAAVAAGAD
HCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHCCCC
TFDCVSPSRVARNAAVYTRDGRVNITGARYRRDFTPIDAECDCYTCAHYTRAYMHHLFKA
CCCCCCHHHHHCCCEEEEECCEEEEEHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KEILASTLATIHNERFTIGLVDRIRASIVEGCFQELREDTLGRYYR
HHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA