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The map label for this gene is ycdT [H]

Identifier: 169627229

GI number: 169627229

Start: 122517

End: 123587

Strand: Direct

Name: ycdT [H]

Synonym: MAB_0124

Alternate gene names: 169627229

Gene position: 122517-123587 (Clockwise)

Preceding gene: 169627228

Following gene: 169627235

Centisome position: 2.42

GC content: 64.89

Gene sequence:

>1071_bases
GTGGGCGCCGGCGAGATGACGTTCGGTCAGGAGTACCGATTCGCGACGCGCGCGCTCACGGTCACTCGCAACCTGGTGCC
GCTCAAAATCGCGATCGGACTGCTGTGCCTGTCGATTGTGGTGTTGGGGACACTCATCCAGTTCCACCCGCTGGGACCGC
ATGGGTTGTGGCCGAGGCTTATTCACGGTGTGCTGGTGGCGTCCGCACTTGTGGTCGGGATGTGCTGGGTTGTGCTGCCC
TGGCCCAGGCGGCGTGTGGCGATCGCATTCGTCTGGTGGGCCGACATCTCGATGGTCGTCGGAGCCTGCACGCTGTCGGC
ACCCGCATCGCGACTGGGCGCCCTCGCGCATATGGGCCTGATCGGAGTTTTCGCCGCGTTTCTGCTGGGATGGCGAGTGT
TGGCGGTGCACTGCGTCTTCGCGACGGTCGCGATCTTCGGCCTCATGGGCTGGAACGTCCGACTCGGCGAGGCGACGTGG
TTCGATCAGTACGTCTACAGTGCGCCCGCACTGTCCTCGGTTGTCCTGCTACCCATCATTATTCAGGTCGTCGTCGAGGG
CGGTAGACGCTCCCTGAAGGCTATATCGCTTGCTGCGCATCAAGATCCGCTGACCGGGCTATTGAACCGTCGTGGCGTAC
AGGTGGCGCTGAGCTCCCTGCTGTCCGGGCAGCGGGCAACCCGAATTGTCGCGGTGGTGCTCGTCGACGTGGACGAGCTG
AAAGAGCTCAACGACAGGCTGGGGCACGACGCCGGAGACGAATCCATCCGCGCCGTCGCGGCAATGCTGGCCACCACGAC
CCGGGCCAGTGATCTCACCGCGCGTATGGGCGGCGATGAGTTCATGGTGGTGGGCTTCTTCGACCATGCCGATGAGGCGA
CAGGCCTCATCGAACGCATCGGGGCCAGGCGATTCGGCATCGGTCCACACCGGGCCAGCGTCAGCATGGGATCTGCCATG
CGATCCACCAGCGTGGCCGGCTTCGACTTCGAGTCGCTGCGCCGGGCGGCGGATGAGGAGCTGCGGGGAGCAAAGATCGA
GCGCCGCAACGGCTTTCACGCGGTTCCCTAG

Upstream 100 bases:

>100_bases
CCAAGTCCTGGTTGCAGCGAGGGCTTACCGGTACCACGTTGTAACCAAATTTCTCGCGCATTCGCTGTCGGTGCGGCACC
GCGCGCTCATAATCACCACT

Downstream 100 bases:

>100_bases
TGCAGGTAGCTCACGGGCAAGGTGGCCGTCAAGAACCTGCGGGCATCGAATTCGTCCACGTAGGGAGCTAACGCAGCGTC
GGGTTCCAAACCGTACCGGT

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MGAGEMTFGQEYRFATRALTVTRNLVPLKIAIGLLCLSIVVLGTLIQFHPLGPHGLWPRLIHGVLVASALVVGMCWVVLP
WPRRRVAIAFVWWADISMVVGACTLSAPASRLGALAHMGLIGVFAAFLLGWRVLAVHCVFATVAIFGLMGWNVRLGEATW
FDQYVYSAPALSSVVLLPIIIQVVVEGGRRSLKAISLAAHQDPLTGLLNRRGVQVALSSLLSGQRATRIVAVVLVDVDEL
KELNDRLGHDAGDESIRAVAAMLATTTRASDLTARMGGDEFMVVGFFDHADEATGLIERIGARRFGIGPHRASVSMGSAM
RSTSVAGFDFESLRRAADEELRGAKIERRNGFHAVP

Sequences:

>Translated_356_residues
MGAGEMTFGQEYRFATRALTVTRNLVPLKIAIGLLCLSIVVLGTLIQFHPLGPHGLWPRLIHGVLVASALVVGMCWVVLP
WPRRRVAIAFVWWADISMVVGACTLSAPASRLGALAHMGLIGVFAAFLLGWRVLAVHCVFATVAIFGLMGWNVRLGEATW
FDQYVYSAPALSSVVLLPIIIQVVVEGGRRSLKAISLAAHQDPLTGLLNRRGVQVALSSLLSGQRATRIVAVVLVDVDEL
KELNDRLGHDAGDESIRAVAAMLATTTRASDLTARMGGDEFMVVGFFDHADEATGLIERIGARRFGIGPHRASVSMGSAM
RSTSVAGFDFESLRRAADEELRGAKIERRNGFHAVP
>Mature_355_residues
GAGEMTFGQEYRFATRALTVTRNLVPLKIAIGLLCLSIVVLGTLIQFHPLGPHGLWPRLIHGVLVASALVVGMCWVVLPW
PRRRVAIAFVWWADISMVVGACTLSAPASRLGALAHMGLIGVFAAFLLGWRVLAVHCVFATVAIFGLMGWNVRLGEATWF
DQYVYSAPALSSVVLLPIIIQVVVEGGRRSLKAISLAAHQDPLTGLLNRRGVQVALSSLLSGQRATRIVAVVLVDVDELK
ELNDRLGHDAGDESIRAVAAMLATTTRASDLTARMGGDEFMVVGFFDHADEATGLIERIGARRFGIGPHRASVSMGSAMR
STSVAGFDFESLRRAADEELRGAKIERRNGFHAVP

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=224, Percent_Identity=28.125, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI145693134, Length=157, Percent_Identity=36.3057324840764, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788381, Length=104, Percent_Identity=40.3846153846154, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786584, Length=202, Percent_Identity=30.1980198019802, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081977, Length=126, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87081881, Length=179, Percent_Identity=31.2849162011173, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082007, Length=167, Percent_Identity=30.5389221556886, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081974, Length=138, Percent_Identity=31.8840579710145, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787802, Length=209, Percent_Identity=26.3157894736842, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787541, Length=189, Percent_Identity=27.5132275132275, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 38353; Mature: 38222

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAGEMTFGQEYRFATRALTVTRNLVPLKIAIGLLCLSIVVLGTLIQFHPLGPHGLWPRL
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
IHGVLVASALVVGMCWVVLPWPRRRVAIAFVWWADISMVVGACTLSAPASRLGALAHMGL
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
IGVFAAFLLGWRVLAVHCVFATVAIFGLMGWNVRLGEATWFDQYVYSAPALSSVVLLPII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHCCCHHHHHHHHHHH
IQVVVEGGRRSLKAISLAAHQDPLTGLLNRRGVQVALSSLLSGQRATRIVAVVLVDVDEL
HHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHEECHHHH
KELNDRLGHDAGDESIRAVAAMLATTTRASDLTARMGGDEFMVVGFFDHADEATGLIERI
HHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHH
GARRFGIGPHRASVSMGSAMRSTSVAGFDFESLRRAADEELRGAKIERRNGFHAVP
CCHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEECCCCCCCCCC
>Mature Secondary Structure 
GAGEMTFGQEYRFATRALTVTRNLVPLKIAIGLLCLSIVVLGTLIQFHPLGPHGLWPRL
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
IHGVLVASALVVGMCWVVLPWPRRRVAIAFVWWADISMVVGACTLSAPASRLGALAHMGL
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
IGVFAAFLLGWRVLAVHCVFATVAIFGLMGWNVRLGEATWFDQYVYSAPALSSVVLLPII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHCCCHHHHHHHHHHH
IQVVVEGGRRSLKAISLAAHQDPLTGLLNRRGVQVALSSLLSGQRATRIVAVVLVDVDEL
HHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHEECHHHH
KELNDRLGHDAGDESIRAVAAMLATTTRASDLTARMGGDEFMVVGFFDHADEATGLIERI
HHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHH
GARRFGIGPHRASVSMGSAMRSTSVAGFDFESLRRAADEELRGAKIERRNGFHAVP
CCHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]