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The map label for this gene is ytwF [H]

Identifier: 169627226

GI number: 169627226

Start: 120051

End: 120377

Strand: Direct

Name: ytwF [H]

Synonym: MAB_0121

Alternate gene names: 169627226

Gene position: 120051-120377 (Clockwise)

Preceding gene: 169627225

Following gene: 169627227

Centisome position: 2.37

GC content: 62.08

Gene sequence:

>327_bases
ATGAGCTTCCCTACTGGTGACGACGTGCCACAGGTCGGCATCGACGAGATTTCGGCCGCGCTGAGTCTTGGTGTCAAGTT
GCTTGACGTTCGTGAGGACGACGAGTGGGCGGCCGGGCACATCGAGGGCGCGCAACATATACCGCTCGGCGATGTGCCCT
CCCGAATGGACGAGCTCGACCCGGATGCGCCGCTATGGGTGATCTGCCACGCCGGAGGACGTTCTCAGCGTGCCGCCGCA
TACCTGAACCGCAATGGTTTCGACGTCAGCAACGTCTCGGGAGGCATGCTGGCATGGGTTCAGGCAGGTAAGCCGACTAT
CAGCTGA

Upstream 100 bases:

>100_bases
TGGATAGTGTGGATAAGCCGACATTGCTGTGGCGTGCGTCACACCACGAAAAGTTGCGGGCGCCGCGCGTGTCATGCCGA
TCGAGTGACAGGAATGAACG

Downstream 100 bases:

>100_bases
TTTCCTGGCGATCGGGCTCGTACGCGGGCTTCAGCGCACTACTCGCTGCCGGTTACTCCCGTAGGCCGTTACGCTGGACG
AATGATCCAGGTGTGCTCCC

Product: rhodanese-like protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 108; Mature: 107

Protein sequence:

>108_residues
MSFPTGDDVPQVGIDEISAALSLGVKLLDVREDDEWAAGHIEGAQHIPLGDVPSRMDELDPDAPLWVICHAGGRSQRAAA
YLNRNGFDVSNVSGGMLAWVQAGKPTIS

Sequences:

>Translated_108_residues
MSFPTGDDVPQVGIDEISAALSLGVKLLDVREDDEWAAGHIEGAQHIPLGDVPSRMDELDPDAPLWVICHAGGRSQRAAA
YLNRNGFDVSNVSGGMLAWVQAGKPTIS
>Mature_107_residues
SFPTGDDVPQVGIDEISAALSLGVKLLDVREDDEWAAGHIEGAQHIPLGDVPSRMDELDPDAPLWVICHAGGRSQRAAAY
LNRNGFDVSNVSGGMLAWVQAGKPTIS

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 11452; Mature: 11321

Theoretical pI: Translated: 4.21; Mature: 4.21

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFPTGDDVPQVGIDEISAALSLGVKLLDVREDDEWAAGHIEGAQHIPLGDVPSRMDELD
CCCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHCC
PDAPLWVICHAGGRSQRAAAYLNRNGFDVSNVSGGMLAWVQAGKPTIS
CCCCEEEEECCCCCCCCHHEEECCCCCCCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
SFPTGDDVPQVGIDEISAALSLGVKLLDVREDDEWAAGHIEGAQHIPLGDVPSRMDELD
CCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHCC
PDAPLWVICHAGGRSQRAAAYLNRNGFDVSNVSGGMLAWVQAGKPTIS
CCCCEEEEECCCCCCCCHHEEECCCCCCCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]