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The map label for this gene is gyrB [H]

Identifier: 169627114

GI number: 169627114

Start: 6249

End: 8276

Strand: Direct

Name: gyrB [H]

Synonym: MAB_0006

Alternate gene names: 169627114

Gene position: 6249-8276 (Clockwise)

Preceding gene: 169627113

Following gene: 169627115

Centisome position: 0.12

GC content: 60.9

Gene sequence:

>2028_bases
GTGGCTGCCCAGAAGAAGAGTGCCAAGAGCGAATACAGTGCTGACTCGATCACCATTCTAGAGGGGCTCGAAGCTGTCCG
GAAGCGCCCCGGTATGTATATCGGGTCCACCGGAGAGCGCGGCCTGCACCATCTGATCTGGGAAGTTGTCGACAACTCCG
TTGATGAGGCGATGGCTGGTTACGCCACCACCGTCGAGGTGACGATGCTGGCCGACGGCGGAATCCAGGTCAAGGACGAT
GGCCGCGGTATCCCCGTAGCCATGCACGCCTCGGGTATCCCGACCGTCGATGTCGTCATGACGCAGCTACATGCCGGAGG
TAAGTTCGACTCGGATTCGTACGCGGTTTCCGGTGGTCTGCACGGTGTGGGCATCTCGGTGGTGAACGCGCTTTCCACCA
AGCTCGAGGTGGAGATCTTGCGCGACGGATTCGAGTGGCAACAGGTCTACACGCGTTCCGAGCCAGGCACCCTGCAGAAG
GGCGCAGCGACCAAGAAGACGGGTACCACCGTACGTTTCTGGGCGGACCCGGAGATCTTCGAGACCACCACCTACGACTT
CGAGACGGTGGCGCGACGCCTGCAGGAGCAGGCCTTCCTCAACAAGGGCCTCACCATCAAGCTCACCGATGAGCGCGTGA
GCGATTCCGAGGTGACCGACGAGGTGGTCAGCGACACCGCCGAGGCGCCCAAGAATGCCGAGGAGCAGGCTGCGGAATCG
TCTGCGCCGCACAAGGTTAAGAACCGGGTGTTCCACTACCCGGACGGTCTGGTGGACTTCGTCAAGCACATCAACCGCAC
CAAGTCAGCCATCCACACCACGATCGTCGATTTCTCCGGCAAGGGCGAAGGCCATGAGGTAGAGATCGCGATGCAGTGGA
ATGCCGGCTATTCCGAATCGGTGCACACCTTTGCCAACACCATCAACACCCATGAGGGCGGCACTCACGAAGAAGGTTTC
CGTGCGGCGCTGACCTCGGTGGTCAACAAATACGCCAAGGAGAAGAAGCTCCTCAAGGAGAAGGATTCGAACCTCACCGG
CGATGACATTCGCGAAGGGTTGGCGGCGGTCATCTCGGTGAAGGTCGGTGAGCCACAGTTCGAAGGCCAGACCAAGACCA
AGCTCGGCAACACCGAGGTCAAGTCCTTTGTGCAGAAGGTATGCAACGAGCAGTTGCAGCACTGGTTCGACTCGAACCCG
GCAGATGCTAAAACGGTTGTGAATAAGGCGGTTTCGTCGGCGCAGGCACGCATCGCCGCGCGTAAGGCACGCGAACTGGT
GCGCCGCAAGAGTGCCACCGATATCGGCGGGCTGCCGGGCAAGCTGGCCGACTGCCGTTCGACAGACCCGTCGAAATCGG
AACTGTATGTGGTGGAGGGTGACTCGGCCGGCGGCTCGGCCAAGAGTGGTCGCGACTCGATGTTCCAGGCGATCCTGCCG
CTGCGCGGCAAGATCATCAACGTCGAAAAGGCCCGCATCGACCGGGTTTTGAAGAACACCGAAGTCCAGGCGATTATCAC
GGCCCTGGGCACGGGCATCCACGACGAGTTCGACATCGCCAAGCTGCGGTACCACAAGATCGTGTTGATGGCCGACGCCG
ACGTTGACGGCCAACATATTTCGACGCTGCTGCTTACGCTGTTGTTCCGATTCATGCGTCCGCTGGTTGAACACGGCCAC
ATCTTCTTGGCGCAGCCACCGTTGTACAAGCTCAAGTGGCAGCGCACCCAGCCGGAATTCGCGTATTCGGACCGCGAGCG
CGACGGGCTGATGGAAGCCGGGCTCAAGGCCGGCAAGAAGATCAACAAGGACGACGGCATCCAGCGTTACAAGGGTCTGG
GCGAGATGGATGCCAAGGAGCTGTGGGAGACCACCATGGATCCGTCCGTGCGCGTACTGCGGCAGGTGACACTCGACGAC
GCCGCCGCGGCCGACGAGCTGTTCTCGATTCTGATGGGTGAGGACGTCGAGGCACGCCGCAGCTTCATCACCCGAAACGC
CAAGGACGTGCGCTTCCTCGACGTATAA

Upstream 100 bases:

>100_bases
GGTGCAGACCAGTAGGATGGTCATAAGACGGATCGCTGGGCCCCTGTTATGAAGCGGGCCCACCGGAGTCGTTACTTCCA
GACAGGAGACAAAGTCGATC

Downstream 100 bases:

>100_bases
CCGCGGCGGGGTAACCCGCCCAGGTAGTTCAAGATTCAATTTGGTCTTGAATCTTTTCGTTCCAGCGGAACCGATTCCGG
CGTTGTGCCGTCGTACTTAC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 675; Mature: 674

Protein sequence:

>675_residues
MAAQKKSAKSEYSADSITILEGLEAVRKRPGMYIGSTGERGLHHLIWEVVDNSVDEAMAGYATTVEVTMLADGGIQVKDD
GRGIPVAMHASGIPTVDVVMTQLHAGGKFDSDSYAVSGGLHGVGISVVNALSTKLEVEILRDGFEWQQVYTRSEPGTLQK
GAATKKTGTTVRFWADPEIFETTTYDFETVARRLQEQAFLNKGLTIKLTDERVSDSEVTDEVVSDTAEAPKNAEEQAAES
SAPHKVKNRVFHYPDGLVDFVKHINRTKSAIHTTIVDFSGKGEGHEVEIAMQWNAGYSESVHTFANTINTHEGGTHEEGF
RAALTSVVNKYAKEKKLLKEKDSNLTGDDIREGLAAVISVKVGEPQFEGQTKTKLGNTEVKSFVQKVCNEQLQHWFDSNP
ADAKTVVNKAVSSAQARIAARKARELVRRKSATDIGGLPGKLADCRSTDPSKSELYVVEGDSAGGSAKSGRDSMFQAILP
LRGKIINVEKARIDRVLKNTEVQAIITALGTGIHDEFDIAKLRYHKIVLMADADVDGQHISTLLLTLLFRFMRPLVEHGH
IFLAQPPLYKLKWQRTQPEFAYSDRERDGLMEAGLKAGKKINKDDGIQRYKGLGEMDAKELWETTMDPSVRVLRQVTLDD
AAAADELFSILMGEDVEARRSFITRNAKDVRFLDV

Sequences:

>Translated_675_residues
MAAQKKSAKSEYSADSITILEGLEAVRKRPGMYIGSTGERGLHHLIWEVVDNSVDEAMAGYATTVEVTMLADGGIQVKDD
GRGIPVAMHASGIPTVDVVMTQLHAGGKFDSDSYAVSGGLHGVGISVVNALSTKLEVEILRDGFEWQQVYTRSEPGTLQK
GAATKKTGTTVRFWADPEIFETTTYDFETVARRLQEQAFLNKGLTIKLTDERVSDSEVTDEVVSDTAEAPKNAEEQAAES
SAPHKVKNRVFHYPDGLVDFVKHINRTKSAIHTTIVDFSGKGEGHEVEIAMQWNAGYSESVHTFANTINTHEGGTHEEGF
RAALTSVVNKYAKEKKLLKEKDSNLTGDDIREGLAAVISVKVGEPQFEGQTKTKLGNTEVKSFVQKVCNEQLQHWFDSNP
ADAKTVVNKAVSSAQARIAARKARELVRRKSATDIGGLPGKLADCRSTDPSKSELYVVEGDSAGGSAKSGRDSMFQAILP
LRGKIINVEKARIDRVLKNTEVQAIITALGTGIHDEFDIAKLRYHKIVLMADADVDGQHISTLLLTLLFRFMRPLVEHGH
IFLAQPPLYKLKWQRTQPEFAYSDRERDGLMEAGLKAGKKINKDDGIQRYKGLGEMDAKELWETTMDPSVRVLRQVTLDD
AAAADELFSILMGEDVEARRSFITRNAKDVRFLDV
>Mature_674_residues
AAQKKSAKSEYSADSITILEGLEAVRKRPGMYIGSTGERGLHHLIWEVVDNSVDEAMAGYATTVEVTMLADGGIQVKDDG
RGIPVAMHASGIPTVDVVMTQLHAGGKFDSDSYAVSGGLHGVGISVVNALSTKLEVEILRDGFEWQQVYTRSEPGTLQKG
AATKKTGTTVRFWADPEIFETTTYDFETVARRLQEQAFLNKGLTIKLTDERVSDSEVTDEVVSDTAEAPKNAEEQAAESS
APHKVKNRVFHYPDGLVDFVKHINRTKSAIHTTIVDFSGKGEGHEVEIAMQWNAGYSESVHTFANTINTHEGGTHEEGFR
AALTSVVNKYAKEKKLLKEKDSNLTGDDIREGLAAVISVKVGEPQFEGQTKTKLGNTEVKSFVQKVCNEQLQHWFDSNPA
DAKTVVNKAVSSAQARIAARKARELVRRKSATDIGGLPGKLADCRSTDPSKSELYVVEGDSAGGSAKSGRDSMFQAILPL
RGKIINVEKARIDRVLKNTEVQAIITALGTGIHDEFDIAKLRYHKIVLMADADVDGQHISTLLLTLLFRFMRPLVEHGHI
FLAQPPLYKLKWQRTQPEFAYSDRERDGLMEAGLKAGKKINKDDGIQRYKGLGEMDAKELWETTMDPSVRVLRQVTLDDA
AAADELFSILMGEDVEARRSFITRNAKDVRFLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=660, Percent_Identity=23.9393939393939, Blast_Score=104, Evalue=4e-22,
Organism=Homo sapiens, GI19913408, Length=388, Percent_Identity=29.1237113402062, Blast_Score=96, Evalue=1e-19,
Organism=Escherichia coli, GI48994957, Length=568, Percent_Identity=51.056338028169, Blast_Score=548, Evalue=1e-157,
Organism=Escherichia coli, GI1789408, Length=666, Percent_Identity=38.4384384384384, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17535065, Length=711, Percent_Identity=23.4880450070323, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI212645845, Length=573, Percent_Identity=24.0837696335079, Blast_Score=104, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324241, Length=702, Percent_Identity=24.6438746438746, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI17136538, Length=668, Percent_Identity=24.1017964071856, Blast_Score=105, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 74265; Mature: 74133

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAQKKSAKSEYSADSITILEGLEAVRKRPGMYIGSTGERGLHHLIWEVVDNSVDEAMAG
CCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHHHC
YATTVEVTMLADGGIQVKDDGRGIPVAMHASGIPTVDVVMTQLHAGGKFDSDSYAVSGGL
CEEEEEEEEEECCCEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGISVVNALSTKLEVEILRDGFEWQQVYTRSEPGTLQKGAATKKTGTTVRFWADPEIF
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCHH
ETTTYDFETVARRLQEQAFLNKGLTIKLTDERVSDSEVTDEVVSDTAEAPKNAEEQAAES
CCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHC
SAPHKVKNRVFHYPDGLVDFVKHINRTKSAIHTTIVDFSGKGEGHEVEIAMQWNAGYSES
CCCHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEEEECCCCCHH
VHTFANTINTHEGGTHEEGFRAALTSVVNKYAKEKKLLKEKDSNLTGDDIREGLAAVISV
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEE
KVGEPQFEGQTKTKLGNTEVKSFVQKVCNEQLQHWFDSNPADAKTVVNKAVSSAQARIAA
ECCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
RKARELVRRKSATDIGGLPGKLADCRSTDPSKSELYVVEGDSAGGSAKSGRDSMFQAILP
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
LRGKIINVEKARIDRVLKNTEVQAIITALGTGIHDEFDIAKLRYHKIVLMADADVDGQHI
CCCEEEEHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHEEEEEEEEEECCCCCHHHH
STLLLTLLFRFMRPLVEHGHIFLAQPPLYKLKWQRTQPEFAYSDRERDGLMEAGLKAGKK
HHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCC
INKDDGIQRYKGLGEMDAKELWETTMDPSVRVLRQVTLDDAAAADELFSILMGEDVEARR
CCCCHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
SFITRNAKDVRFLDV
HHHHCCCCCEEEECC
>Mature Secondary Structure 
AAQKKSAKSEYSADSITILEGLEAVRKRPGMYIGSTGERGLHHLIWEVVDNSVDEAMAG
CCCCCCCHHHCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHHHC
YATTVEVTMLADGGIQVKDDGRGIPVAMHASGIPTVDVVMTQLHAGGKFDSDSYAVSGGL
CEEEEEEEEEECCCEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGISVVNALSTKLEVEILRDGFEWQQVYTRSEPGTLQKGAATKKTGTTVRFWADPEIF
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCHH
ETTTYDFETVARRLQEQAFLNKGLTIKLTDERVSDSEVTDEVVSDTAEAPKNAEEQAAES
CCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHC
SAPHKVKNRVFHYPDGLVDFVKHINRTKSAIHTTIVDFSGKGEGHEVEIAMQWNAGYSES
CCCHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEEEECCCCCHH
VHTFANTINTHEGGTHEEGFRAALTSVVNKYAKEKKLLKEKDSNLTGDDIREGLAAVISV
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEE
KVGEPQFEGQTKTKLGNTEVKSFVQKVCNEQLQHWFDSNPADAKTVVNKAVSSAQARIAA
ECCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
RKARELVRRKSATDIGGLPGKLADCRSTDPSKSELYVVEGDSAGGSAKSGRDSMFQAILP
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
LRGKIINVEKARIDRVLKNTEVQAIITALGTGIHDEFDIAKLRYHKIVLMADADVDGQHI
CCCEEEEHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHEEEEEEEEEECCCCCHHHH
STLLLTLLFRFMRPLVEHGHIFLAQPPLYKLKWQRTQPEFAYSDRERDGLMEAGLKAGKK
HHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCC
INKDDGIQRYKGLGEMDAKELWETTMDPSVRVLRQVTLDDAAAADELFSILMGEDVEARR
CCCCHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
SFITRNAKDVRFLDV
HHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7981652 [H]