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The map label for this gene is recF [H]

Identifier: 169627112

GI number: 169627112

Start: 4275

End: 5480

Strand: Direct

Name: recF [H]

Synonym: MAB_0004

Alternate gene names: 169627112

Gene position: 4275-5480 (Clockwise)

Preceding gene: 169627111

Following gene: 169627113

Centisome position: 0.08

GC content: 63.6

Gene sequence:

>1206_bases
GTGGCCACGCGGTCAAGCGCGTGGGCCTTTCCGGCTGAGGGAGGCGCGCGGACCGAGGTGTATGTACGACAGCTGGGATT
ACGGGACTTCCGGTCATGGGAGCGTGTCAACCTGGAACTCGAGCCCGGCCGCACGGTGTTCGTCGGACCGAACGGCTACG
GCAAGACGAATCTTGTTGAGGCACTCTGGTATTCATCGACGTTGGGTTCGCACCGGGTAGCCACGGACGCGCCCTTGATT
CGTACTGGAGCCGAGCGCGCCGTGGTATCGACCATTGTGGTCAATGATGGTCGTGAGTTGGCGATCGATCTGGAGATTGC
CGCGGGGCGTGCCAATAAGGCCAGGGTCAACCGATCTCCGGTGCGCAGTCCCCGCGAGGTTTTGGGGATTTTGCACGCGG
TGCTCTTCGCGCCCGAAGATTTGTCCTTGGTGCGTGGAGATCCCGCGGATCGTCGCCGATTCCTGGACGATCTGCTGATT
CAACGGCGTCCGCGGATGGCCGGGGTGCGTGCCGACTACGACAAGGTGCTGCGGCAGCGGACCGCGCTGCTGAAGACCGC
CATGGCGGCATTGCGGCAACGTCATGATCAAAGTGTGCTGGACACGTTGGACGTGTGGGATGGGCATCTGGTGGCACACG
GCGCCGAATTGCTTTCGGCGCGTATCGAATTGGTGGGCGAGCTGCACCCGCTGGTGGAAAAGTCGTATCAGCTCCTGGCT
CCGGCGTCACGCCCCGCGGATATCGCGTACCGCAGCAGTGTCGAGGGCGTCGAGGCAGGGCTGTCTGTGGAGCATCTGGC
GGATGCGTTGCAGGCCGGATTGGTGGCGAAACGCTCGGCGGAAATAGAACGTGGCGTCTGTCTGGTCGGTCCGCACCGCG
ACGACCTGGAATTGCGGCTGGGAGACGGGCCTGCGAAGGGATTCGCCAGTCACGGTGAATCGTGGTCCTTTGCGTTGGCA
CTGCGGCTGGCGGCATTCGACCTGCTGCGTGCCGACGGCACGGATCCGGTGTTAATGCTTGATGACGTTTTCGCCGAGCT
CGACGGTGCCCGGCGAAGGTCCTTGGCCACGGTGGCCGCAGATGCCGAGCAAGTTTTGGTGACGGCGGCGGTCCCGGACG
ACGTTCCTGAGGAATTGTCGGCACGCACGGTTACCGTTGAGGTGCAGGAAGGCCCCGCGGGACGAAAGTCGGTGGCACAT
GAGTGA

Upstream 100 bases:

>100_bases
CCGGCGCCGGTCATCGCGGCCTCGCTATTCGCGAGATTCGCGTCACGGCAAGAGGACTCACCGGCAATGAAGGCTGTCTC
GGCATTGCGCAACCAGTTTG

Downstream 100 bases:

>100_bases
GGAAGAGGCCTGGCCGCCGGAGCACCTGGCCGGGGTGAAGGGCATGGATTTGGTGCGCCGGGTCTTGGAGGAGGCCCGGG
GCGCGGCCAAGCAGCAAGGG

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MATRSSAWAFPAEGGARTEVYVRQLGLRDFRSWERVNLELEPGRTVFVGPNGYGKTNLVEALWYSSTLGSHRVATDAPLI
RTGAERAVVSTIVVNDGRELAIDLEIAAGRANKARVNRSPVRSPREVLGILHAVLFAPEDLSLVRGDPADRRRFLDDLLI
QRRPRMAGVRADYDKVLRQRTALLKTAMAALRQRHDQSVLDTLDVWDGHLVAHGAELLSARIELVGELHPLVEKSYQLLA
PASRPADIAYRSSVEGVEAGLSVEHLADALQAGLVAKRSAEIERGVCLVGPHRDDLELRLGDGPAKGFASHGESWSFALA
LRLAAFDLLRADGTDPVLMLDDVFAELDGARRRSLATVAADAEQVLVTAAVPDDVPEELSARTVTVEVQEGPAGRKSVAH
E

Sequences:

>Translated_401_residues
MATRSSAWAFPAEGGARTEVYVRQLGLRDFRSWERVNLELEPGRTVFVGPNGYGKTNLVEALWYSSTLGSHRVATDAPLI
RTGAERAVVSTIVVNDGRELAIDLEIAAGRANKARVNRSPVRSPREVLGILHAVLFAPEDLSLVRGDPADRRRFLDDLLI
QRRPRMAGVRADYDKVLRQRTALLKTAMAALRQRHDQSVLDTLDVWDGHLVAHGAELLSARIELVGELHPLVEKSYQLLA
PASRPADIAYRSSVEGVEAGLSVEHLADALQAGLVAKRSAEIERGVCLVGPHRDDLELRLGDGPAKGFASHGESWSFALA
LRLAAFDLLRADGTDPVLMLDDVFAELDGARRRSLATVAADAEQVLVTAAVPDDVPEELSARTVTVEVQEGPAGRKSVAH
E
>Mature_400_residues
ATRSSAWAFPAEGGARTEVYVRQLGLRDFRSWERVNLELEPGRTVFVGPNGYGKTNLVEALWYSSTLGSHRVATDAPLIR
TGAERAVVSTIVVNDGRELAIDLEIAAGRANKARVNRSPVRSPREVLGILHAVLFAPEDLSLVRGDPADRRRFLDDLLIQ
RRPRMAGVRADYDKVLRQRTALLKTAMAALRQRHDQSVLDTLDVWDGHLVAHGAELLSARIELVGELHPLVEKSYQLLAP
ASRPADIAYRSSVEGVEAGLSVEHLADALQAGLVAKRSAEIERGVCLVGPHRDDLELRLGDGPAKGFASHGESWSFALAL
RLAAFDLLRADGTDPVLMLDDVFAELDGARRRSLATVAADAEQVLVTAAVPDDVPEELSARTVTVEVQEGPAGRKSVAHE

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=380, Percent_Identity=28.1578947368421, Blast_Score=101, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 43589; Mature: 43458

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRSSAWAFPAEGGARTEVYVRQLGLRDFRSWERVNLELEPGRTVFVGPNGYGKTNLVE
CCCCCCCCCCCCCCCCHHHHHHHHHCHHHHCCHHHEEEEECCCCEEEECCCCCCCHHHHH
ALWYSSTLGSHRVATDAPLIRTGAERAVVSTIVVNDGRELAIDLEIAAGRANKARVNRSP
HHHHHHCCCCCCCCCCCCHHHCCCHHHEEEEEEEECCCEEEEEEEEECCCCCHHCCCCCC
VRSPREVLGILHAVLFAPEDLSLVRGDPADRRRFLDDLLIQRRPRMAGVRADYDKVLRQR
CCCHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
TALLKTAMAALRQRHDQSVLDTLDVWDGHLVAHGAELLSARIELVGELHPLVEKSYQLLA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PASRPADIAYRSSVEGVEAGLSVEHLADALQAGLVAKRSAEIERGVCLVGPHRDDLELRL
CCCCCCCHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEE
GDGPAKGFASHGESWSFALALRLAAFDLLRADGTDPVLMLDDVFAELDGARRRSLATVAA
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCHHHHHHHHHHC
DAEQVLVTAAVPDDVPEELSARTVTVEVQEGPAGRKSVAHE
CCCCEEEEEECCCCCHHHHCCEEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
ATRSSAWAFPAEGGARTEVYVRQLGLRDFRSWERVNLELEPGRTVFVGPNGYGKTNLVE
CCCCCCCCCCCCCCCHHHHHHHHHCHHHHCCHHHEEEEECCCCEEEECCCCCCCHHHHH
ALWYSSTLGSHRVATDAPLIRTGAERAVVSTIVVNDGRELAIDLEIAAGRANKARVNRSP
HHHHHHCCCCCCCCCCCCHHHCCCHHHEEEEEEEECCCEEEEEEEEECCCCCHHCCCCCC
VRSPREVLGILHAVLFAPEDLSLVRGDPADRRRFLDDLLIQRRPRMAGVRADYDKVLRQR
CCCHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
TALLKTAMAALRQRHDQSVLDTLDVWDGHLVAHGAELLSARIELVGELHPLVEKSYQLLA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PASRPADIAYRSSVEGVEAGLSVEHLADALQAGLVAKRSAEIERGVCLVGPHRDDLELRL
CCCCCCCHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEE
GDGPAKGFASHGESWSFALALRLAAFDLLRADGTDPVLMLDDVFAELDGARRRSLATVAA
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCHHHHHHHHHHC
DAEQVLVTAAVPDDVPEELSARTVTVEVQEGPAGRKSVAHE
CCCCEEEEEECCCCCHHHHCCEEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8878580 [H]