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The map label for this gene is amiB

Identifier: 16767604

GI number: 16767604

Start: 4600364

End: 4601683

Strand: Direct

Name: amiB

Synonym: STM4358

Alternate gene names: 16767604

Gene position: 4600364-4601683 (Clockwise)

Preceding gene: 16767603

Following gene: 16767605

Centisome position: 94.71

GC content: 57.65

Gene sequence:

>1320_bases
ATGATTTATCGCATCAAAAATGCCGTAATAGCAGCCTTAATCCTGCTGTGCGCGCAGGCGGGCGCCGCCAGTCTCTCTGA
TATTCAGGTGTCCAACGGCGAGCAGCAGGCGCGCATTACGCTGAGTTTTATCGGCGAGCCGGAATATGCTTACTCACAGG
ACGGTAAACGGACTGTGGCGCTGGATATCAGGCAGACCGGCGTGATTCAGGGGCTGCCGCTTCAGTTTAGCGGTAACAAT
CTGGTAAAAACGATCCGTGCTGGAACGCCGAAAGATGCGCAATCTTTACGGTTGCTTGTCGATCTGACGGAAAATGGTAA
AACCGAGGCGGTGAAGCGGCAAAACGGCGGCAACTATACGGTCATTTTCACCATTAATGCCGATGTTCCGCCGCCACCGC
CGCCTGTCGTCGCGAAACGGGTTGAAAGCGCGCCTCGCCCGACGGAGCCGGCCCGTAATCCGTTTAAATCTTCTGATGAT
CGCCTGACCGGCGTAACCAGCAGCAATACCGTTACGCGGCCTGCGGCGCGCGCGTCGGCAGGCGCTGGCGATAAAGTGGT
GATTGCTATTGATGCCGGACATGGAGGCCAGGACCCCGGCGCGATAGGCCCGGGCGGTACGCGCGAGAAAAATGTCACCA
TCGCCATCGCGCGTAAGTTACGCACATTACTGAATAACGATCCGATGTTTAAAGGCGTGCTGACGCGTGACGGCGACTAT
TTTATTTCGGTGATGGGGCGGTCTGATGTGGCGCGTAAGCAAAACGCCAACTTTCTTGTCTCTATTCATGCGGATGCCGC
GCCAAATCGCGACGCCACCGGCGCCTCCGTCTGGGTATTGTCCAATCGTCGCGCGAACAGCGAAATGGCAAACTGGCTTG
AGCAGCATGAGAAGCAATCCGAACTGTTAGGCGGCGCTGGCGATGTGCTGGCGAACAGTCAGTCAGACCCTTACCTGAGC
CAGGCGGTACTGGATTTGCAATTCGGTCATTCGCAGCGGGTAGGGTATGATGTGGCGACGAACGTACTAAGCCAACTCGA
CGGCGTGGGGTCGCTGCATAAACGCCGCCCGGAACACGCTAGCCTGGGCGTGTTGCGTTCGCCGGATATCCCGTCCATTT
TGGTGGAGACGGGCTTTATCAGTAATCACGGCGAAGAGCGATTGCTGGCGAGCGACCGCTATCAGCAGCAGATTGCTGAC
GCTATATACCGGGGGTTGCGTAAGTACTTTGCAGCGCATCCGATCCAGTCTGCGCCGCAGGGCGGCCCAGGCCAGACGGC
CAGTACCAACCAGCCTGGCGCCATAACCGCCGCGAATTAA

Upstream 100 bases:

>100_bases
GATTACCAGGCGCAGGGGCGTGAGGCTCGCGTAAGCGCGGTATCCTCATCGGGTCATTCGTTACTGGCGCGTTTAGCCGA
TTAGCTATAAAGGTGGCGGG

Downstream 100 bases:

>100_bases
GGAGATTTCATGCCGATTCAGGTTCTGCCGCCGCAGCTTGCGAACCAAATCGCCGCTGGCGAAGTGGTGGAACGCCCTGC
GTCGGTTGTTAAAGAGCTGG

Product: N-acetylmuramoyl-l-alanine amidase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MIYRIKNAVIAALILLCAQAGAASLSDIQVSNGEQQARITLSFIGEPEYAYSQDGKRTVALDIRQTGVIQGLPLQFSGNN
LVKTIRAGTPKDAQSLRLLVDLTENGKTEAVKRQNGGNYTVIFTINADVPPPPPPVVAKRVESAPRPTEPARNPFKSSDD
RLTGVTSSNTVTRPAARASAGAGDKVVIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNNDPMFKGVLTRDGDY
FISVMGRSDVARKQNANFLVSIHADAAPNRDATGASVWVLSNRRANSEMANWLEQHEKQSELLGGAGDVLANSQSDPYLS
QAVLDLQFGHSQRVGYDVATNVLSQLDGVGSLHKRRPEHASLGVLRSPDIPSILVETGFISNHGEERLLASDRYQQQIAD
AIYRGLRKYFAAHPIQSAPQGGPGQTASTNQPGAITAAN

Sequences:

>Translated_439_residues
MIYRIKNAVIAALILLCAQAGAASLSDIQVSNGEQQARITLSFIGEPEYAYSQDGKRTVALDIRQTGVIQGLPLQFSGNN
LVKTIRAGTPKDAQSLRLLVDLTENGKTEAVKRQNGGNYTVIFTINADVPPPPPPVVAKRVESAPRPTEPARNPFKSSDD
RLTGVTSSNTVTRPAARASAGAGDKVVIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNNDPMFKGVLTRDGDY
FISVMGRSDVARKQNANFLVSIHADAAPNRDATGASVWVLSNRRANSEMANWLEQHEKQSELLGGAGDVLANSQSDPYLS
QAVLDLQFGHSQRVGYDVATNVLSQLDGVGSLHKRRPEHASLGVLRSPDIPSILVETGFISNHGEERLLASDRYQQQIAD
AIYRGLRKYFAAHPIQSAPQGGPGQTASTNQPGAITAAN
>Mature_439_residues
MIYRIKNAVIAALILLCAQAGAASLSDIQVSNGEQQARITLSFIGEPEYAYSQDGKRTVALDIRQTGVIQGLPLQFSGNN
LVKTIRAGTPKDAQSLRLLVDLTENGKTEAVKRQNGGNYTVIFTINADVPPPPPPVVAKRVESAPRPTEPARNPFKSSDD
RLTGVTSSNTVTRPAARASAGAGDKVVIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNNDPMFKGVLTRDGDY
FISVMGRSDVARKQNANFLVSIHADAAPNRDATGASVWVLSNRRANSEMANWLEQHEKQSELLGGAGDVLANSQSDPYLS
QAVLDLQFGHSQRVGYDVATNVLSQLDGVGSLHKRRPEHASLGVLRSPDIPSILVETGFISNHGEERLLASDRYQQQIAD
AIYRGLRKYFAAHPIQSAPQGGPGQTASTNQPGAITAAN

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family

Homologues:

Organism=Escherichia coli, GI1790611, Length=435, Percent_Identity=83.448275862069, Blast_Score=731, Evalue=0.0,
Organism=Escherichia coli, GI87082163, Length=232, Percent_Identity=41.3793103448276, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1788776, Length=246, Percent_Identity=40.2439024390244, Blast_Score=177, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMIB_SALTY (P26366)

Other databases:

- EMBL:   AE006468
- EMBL:   M29687
- RefSeq:   NP_463219.1
- ProteinModelPortal:   P26366
- SMR:   P26366
- PRIDE:   P26366
- GeneID:   1255884
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM4358
- NMPDR:   fig|99287.1.peg.4192
- HOGENOM:   HBG656726
- OMA:   NSQADPY
- ProtClustDB:   PRK10431
- BioCyc:   STYP99287:STM4358-MONOMER
- BRENDA:   3.5.1.28
- InterPro:   IPR002508
- Gene3D:   G3DSA:3.40.630.40
- SMART:   SM00646

Pfam domain/function: PF01520 Amidase_3

EC number: =3.5.1.28

Molecular weight: Translated: 46790; Mature: 46790

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYRIKNAVIAALILLCAQAGAASLSDIQVSNGEQQARITLSFIGEPEYAYSQDGKRTVA
CEEEEHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEEEEECCCCCCCCCCCCEEEE
LDIRQTGVIQGLPLQFSGNNLVKTIRAGTPKDAQSLRLLVDLTENGKTEAVKRQNGGNYT
EEEECCCCEECCCEEECCCHHEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHCCCCCEE
VIFTINADVPPPPPPVVAKRVESAPRPTEPARNPFKSSDDRLTGVTSSNTVTRPAARASA
EEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHCC
GAGDKVVIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNNDPMFKGVLTRDGDY
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCEEEEEECCCCE
FISVMGRSDVARKQNANFLVSIHADAAPNRDATGASVWVLSNRRANSEMANWLEQHEKQS
EEEEECCCHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
ELLGGAGDVLANSQSDPYLSQAVLDLQFGHSQRVGYDVATNVLSQLDGVGSLHKRRPEHA
HHCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCC
SLGVLRSPDIPSILVETGFISNHGEERLLASDRYQQQIADAIYRGLRKYFAAHPIQSAPQ
CCCEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GGPGQTASTNQPGAITAAN
CCCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MIYRIKNAVIAALILLCAQAGAASLSDIQVSNGEQQARITLSFIGEPEYAYSQDGKRTVA
CEEEEHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEEEEECCCCCCCCCCCCEEEE
LDIRQTGVIQGLPLQFSGNNLVKTIRAGTPKDAQSLRLLVDLTENGKTEAVKRQNGGNYT
EEEECCCCEECCCEEECCCHHEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHCCCCCEE
VIFTINADVPPPPPPVVAKRVESAPRPTEPARNPFKSSDDRLTGVTSSNTVTRPAARASA
EEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHCC
GAGDKVVIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNNDPMFKGVLTRDGDY
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCEEEEEECCCCE
FISVMGRSDVARKQNANFLVSIHADAAPNRDATGASVWVLSNRRANSEMANWLEQHEKQS
EEEEECCCHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
ELLGGAGDVLANSQSDPYLSQAVLDLQFGHSQRVGYDVATNVLSQLDGVGSLHKRRPEHA
HHCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCC
SLGVLRSPDIPSILVETGFISNHGEERLLASDRYQQQIADAIYRGLRKYFAAHPIQSAPQ
CCCEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GGPGQTASTNQPGAITAAN
CCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 2676972