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The map label for this gene is basS

Identifier: 16767541

GI number: 16767541

Start: 4532639

End: 4533709

Strand: Reverse

Name: basS

Synonym: STM4291

Alternate gene names: 16767541

Gene position: 4533709-4532639 (Counterclockwise)

Preceding gene: 16767542

Following gene: 16767539

Centisome position: 93.34

GC content: 54.62

Gene sequence:

>1071_bases
ATGCGTTTTCAGCGAAGAGCGATGACCCTTCGCCAGCGTTTAATGCTGACAATTGGTCTTATTCTGCTGGTGTTCCAGTT
AATCAGTACCTTCTGGTTATGGCATGAAAGCACTGAGCAAATCCAATTGTTCGAGCAGGCGCTGCGGGATAATCGCAACA
ACGATCGCCATATCATGCACGAAATTCGCGAGGCGGTCGCCAGCCTGATCGTCCCCGGCGTATTTATGGTTAGCCTGACG
CTGCTAATTTGTTACCAGGCGGTACGGCGTATTACCCGTCCGCTCGCCGAACTGCAAAAAGAGCTGGAAGCGCGGACGGC
GGATAATCTGGCGCCGATCGCCATTCACAGCTCCACGCTTGAGATTGAGTCCGTCGTCTCGGCGATCAATCAACTGGTTA
CGCGTTTGACCACCACGCTCGACAATGAACGCCTTTTTACCGCCGATGTGGCCCATGAGCTACGCACGCCGCTGTCGGGG
GTGCGTTTGCACCTGGAATTATTGTCAAAAACCCACAATGTTGATGTCGCGCCGCTTATCGCCCGTCTTGACCAGATGAT
GGATAGCGTCTCCCAGCTTCTGCAACTGGCGCGCGTGGGCCAGTCATTCTCTTCCGGGAATTATCAGGAAGTAAAACTGC
TGGAAGATGTGATCCTCCCCTCCTACGATGAGCTGAACACCATGCTGGAAACGCGCCAGCAAACTCTGTTGCTGCCGGAA
AGCGCGGCGGACGTGGTGGTGCGCGGTGATGCGACGTTACTGCGTATGCTGTTGCGAAATCTGGTGGAAAACGCGCATCG
CTATAGCCCTGAAGGAACCCATATCACTATCCACATTAGCGCCGACCCCGACGCTATTATGGCGGTCGAAGACGAGGGGC
CGGGTATTGATGAAAGCAAATGCGGGAAGCTAAGCGAAGCGTTCGTGCGGATGGACAGCCGTTATGGCGGAATTGGCCTG
GGGCTGAGTATCGTCAGCCGCATCACCCAACTACATCAGGGACAGTTTTTCCTGCAAAACCGTACGGAAAGAACAGGCAC
CCGTGCCTGGGTGCTGTTGAAAAAGGCATAA

Upstream 100 bases:

>100_bases
TATACATAATTTGCGCGACAAAGTCGGCAAGTCGCGCATTCGCACGGTTCGCGGGTTTGGCTACATGCTGGTTGCCACTG
AGGAAAGCTAAGTGAGCCTG

Downstream 100 bases:

>100_bases
CAGGCGTTTAATACGTCGTGACCCACACATAGATAAAACTGCTGACAATCAGCACACTACACAGGGTCGTCAAACTTTTA
TAAATATAGCTTTTCATGAC

Product: sensor protein BasS/PmrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MRFQRRAMTLRQRLMLTIGLILLVFQLISTFWLWHESTEQIQLFEQALRDNRNNDRHIMHEIREAVASLIVPGVFMVSLT
LLICYQAVRRITRPLAELQKELEARTADNLAPIAIHSSTLEIESVVSAINQLVTRLTTTLDNERLFTADVAHELRTPLSG
VRLHLELLSKTHNVDVAPLIARLDQMMDSVSQLLQLARVGQSFSSGNYQEVKLLEDVILPSYDELNTMLETRQQTLLLPE
SAADVVVRGDATLLRMLLRNLVENAHRYSPEGTHITIHISADPDAIMAVEDEGPGIDESKCGKLSEAFVRMDSRYGGIGL
GLSIVSRITQLHQGQFFLQNRTERTGTRAWVLLKKA

Sequences:

>Translated_356_residues
MRFQRRAMTLRQRLMLTIGLILLVFQLISTFWLWHESTEQIQLFEQALRDNRNNDRHIMHEIREAVASLIVPGVFMVSLT
LLICYQAVRRITRPLAELQKELEARTADNLAPIAIHSSTLEIESVVSAINQLVTRLTTTLDNERLFTADVAHELRTPLSG
VRLHLELLSKTHNVDVAPLIARLDQMMDSVSQLLQLARVGQSFSSGNYQEVKLLEDVILPSYDELNTMLETRQQTLLLPE
SAADVVVRGDATLLRMLLRNLVENAHRYSPEGTHITIHISADPDAIMAVEDEGPGIDESKCGKLSEAFVRMDSRYGGIGL
GLSIVSRITQLHQGQFFLQNRTERTGTRAWVLLKKA
>Mature_356_residues
MRFQRRAMTLRQRLMLTIGLILLVFQLISTFWLWHESTEQIQLFEQALRDNRNNDRHIMHEIREAVASLIVPGVFMVSLT
LLICYQAVRRITRPLAELQKELEARTADNLAPIAIHSSTLEIESVVSAINQLVTRLTTTLDNERLFTADVAHELRTPLSG
VRLHLELLSKTHNVDVAPLIARLDQMMDSVSQLLQLARVGQSFSSGNYQEVKLLEDVILPSYDELNTMLETRQQTLLLPE
SAADVVVRGDATLLRMLLRNLVENAHRYSPEGTHITIHISADPDAIMAVEDEGPGIDESKCGKLSEAFVRMDSRYGGIGL
GLSIVSRITQLHQGQFFLQNRTERTGTRAWVLLKKA

Specific function: Member of the two-component regulatory system BasS/BasR. Autophosphorylates and activates BasR by phosphorylation. Plays a role in the adaptation of the organism to the host environment, in particular to neutrophils, and therefore it plays a role in virul

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain

Homologues:

Organism=Escherichia coli, GI1790551, Length=355, Percent_Identity=85.9154929577465, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI1789403, Length=276, Percent_Identity=31.5217391304348, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789808, Length=269, Percent_Identity=28.996282527881, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1786600, Length=227, Percent_Identity=30.8370044052863, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786783, Length=303, Percent_Identity=28.0528052805281, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790346, Length=236, Percent_Identity=28.3898305084746, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788393, Length=231, Percent_Identity=29.8701298701299, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788279, Length=296, Percent_Identity=28.7162162162162, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787894, Length=245, Percent_Identity=28.9795918367347, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081816, Length=355, Percent_Identity=24.7887323943662, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790861, Length=278, Percent_Identity=24.8201438848921, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BASS_SALTY (P36557)

Other databases:

- EMBL:   L13395
- EMBL:   AE006468
- PIR:   C40656
- RefSeq:   NP_463156.1
- ProteinModelPortal:   P36557
- PRIDE:   P36557
- GeneID:   1255817
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM4291
- HOGENOM:   HBG416840
- OMA:   ANIITET
- ProtClustDB:   PRK10755
- BioCyc:   STYP99287:STM4291-MONOMER
- BRENDA:   2.7.13.3
- GO:   GO:0009405
- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- Gene3D:   G3DSA:3.30.565.10
- PRINTS:   PR00344
- SMART:   SM00304
- SMART:   SM00387
- SMART:   SM00388

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; SSF55874 ATP_bd_ATPase; SSF47384 His_kin_homodim

EC number: =2.7.13.3

Molecular weight: Translated: 40263; Mature: 40263

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS50885 HAMP; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x12b5b0d8)-; HASH(0x147591a4)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFQRRAMTLRQRLMLTIGLILLVFQLISTFWLWHESTEQIQLFEQALRDNRNNDRHIMH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHH
EIREAVASLIVPGVFMVSLTLLICYQAVRRITRPLAELQKELEARTADNLAPIAIHSSTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHH
EIESVVSAINQLVTRLTTTLDNERLFTADVAHELRTPLSGVRLHLELLSKTHNVDVAPLI
HHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHH
ARLDQMMDSVSQLLQLARVGQSFSSGNYQEVKLLEDVILPSYDELNTMLETRQQTLLLPE
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCC
SAADVVVRGDATLLRMLLRNLVENAHRYSPEGTHITIHISADPDAIMAVEDEGPGIDESK
CCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEECCCCCCCHHH
CGKLSEAFVRMDSRYGGIGLGLSIVSRITQLHQGQFFLQNRTERTGTRAWVLLKKA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCEEEEEEECH
>Mature Secondary Structure
MRFQRRAMTLRQRLMLTIGLILLVFQLISTFWLWHESTEQIQLFEQALRDNRNNDRHIMH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHH
EIREAVASLIVPGVFMVSLTLLICYQAVRRITRPLAELQKELEARTADNLAPIAIHSSTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHH
EIESVVSAINQLVTRLTTTLDNERLFTADVAHELRTPLSGVRLHLELLSKTHNVDVAPLI
HHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHH
ARLDQMMDSVSQLLQLARVGQSFSSGNYQEVKLLEDVILPSYDELNTMLETRQQTLLLPE
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCC
SAADVVVRGDATLLRMLLRNLVENAHRYSPEGTHITIHISADPDAIMAVEDEGPGIDESK
CCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEECCCCCCCHHH
CGKLSEAFVRMDSRYGGIGLGLSIVSRITQLHQGQFFLQNRTERTGTRAWVLLKKA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCEEEEEEECH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609