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The map label for this gene is dsbA

Identifier: 16767262

GI number: 16767262

Start: 4204199

End: 4204822

Strand: Direct

Name: dsbA

Synonym: STM3997

Alternate gene names: 16767262

Gene position: 4204199-4204822 (Clockwise)

Preceding gene: 16767261

Following gene: 16767264

Centisome position: 86.55

GC content: 50.32

Gene sequence:

>624_bases
ATGAAAAAGATTTGGCTGGCGCTGGCTGGTATGGTTTTAGCTTTTAGCGCCTCGGCAGCACAGATCAGCGACGGTAAACA
GTATATCACGCTGGATAAACCGGTCGCTGGCGAACCCCAGGTACTGGAGTTTTTCTCCTTCTACTGCCCACATTGTTATC
AGTTTGAAGAAGTGCTTCATGTGTCTGACAATGTGAAGAAAAAGCTGCCGGAAGGCACCAAAATGACCAAGTACCACGTT
GAGTTCCTGGGGCCGTTGGGCAAGGAGCTCACCCAGGCATGGGCGGTGGCGATGGCGTTGGGTGTAGAAGATAAAGTCAC
GGTCCCGCTGTTTGAAGCCGTACAGAAAACCCAGACAGTACAATCTGCCGCGGATATCCGTAAAGTGTTCGTTGATGCGG
GCGTCAAGGGCGAAGATTACGATGCGGCATGGAACAGCTTCGTGGTGAAATCACTGGTTGCGCAACAGGAGAAAGCCGCG
GCTGACCTGCAACTGCAGGGCGTTCCGGCGATGTTCGTCAATGGCAAATACCAGATTAACCCACAAGGCATGGATACGAG
CAGCATGGATGTTTTTGTTCAGCAGTATGCTGATACTGTGAAATATTTGGTTGATAAAAAATAA

Upstream 100 bases:

>100_bases
TACTGGCAGCGACAGACAACGACTTTTATTGAGCAGACTAAAATTTTGCACGAACCCCCTTTACAATTAACGCCAATGTA
TTAATCGGAGAGAGTTGATC

Downstream 100 bases:

>100_bases
AAAGAACGCCGGTCACTGACCGGCGTTTTTATAAGATGTTTTAATCTCTTCTATCTGTATATCTTTCTTATCCCACAGCG
TATTAAGCCAACGTTGAAAA

Product: periplasmic protein disulfide isomerase I

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MKKIWLALAGMVLAFSASAAQISDGKQYITLDKPVAGEPQVLEFFSFYCPHCYQFEEVLHVSDNVKKKLPEGTKMTKYHV
EFLGPLGKELTQAWAVAMALGVEDKVTVPLFEAVQKTQTVQSAADIRKVFVDAGVKGEDYDAAWNSFVVKSLVAQQEKAA
ADLQLQGVPAMFVNGKYQINPQGMDTSSMDVFVQQYADTVKYLVDKK

Sequences:

>Translated_207_residues
MKKIWLALAGMVLAFSASAAQISDGKQYITLDKPVAGEPQVLEFFSFYCPHCYQFEEVLHVSDNVKKKLPEGTKMTKYHV
EFLGPLGKELTQAWAVAMALGVEDKVTVPLFEAVQKTQTVQSAADIRKVFVDAGVKGEDYDAAWNSFVVKSLVAQQEKAA
ADLQLQGVPAMFVNGKYQINPQGMDTSSMDVFVQQYADTVKYLVDKK
>Mature_207_residues
MKKIWLALAGMVLAFSASAAQISDGKQYITLDKPVAGEPQVLEFFSFYCPHCYQFEEVLHVSDNVKKKLPEGTKMTKYHV
EFLGPLGKELTQAWAVAMALGVEDKVTVPLFEAVQKTQTVQSAADIRKVFVDAGVKGEDYDAAWNSFVVKSLVAQQEKAA
ADLQLQGVPAMFVNGKYQINPQGMDTSSMDVFVQQYADTVKYLVDKK

Specific function: Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. It is required for pilus biogenesis

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain

Homologues:

Organism=Escherichia coli, GI1790291, Length=208, Percent_Identity=86.0576923076923, Blast_Score=376, Evalue=1e-106,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): DSBA_SALEN (P0A2I0)

Other databases:

- EMBL:   AF067152
- ProteinModelPortal:   P0A2I0
- SMR:   P0A2I0
- PRIDE:   P0A2I0
- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335
- Gene3D:   G3DSA:3.40.30.10
- PIRSF:   PIRSF001488

Pfam domain/function: PF01323 DSBA; SSF52833 Thiordxn-like_fd

EC number: NA

Molecular weight: Translated: 22912; Mature: 22912

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00194 THIOREDOXIN_1; PS51352 THIOREDOXIN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIWLALAGMVLAFSASAAQISDGKQYITLDKPVAGEPQVLEFFSFYCPHCYQFEEVLH
CHHHHHHHHHHHHHHCCCHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHH
VSDNVKKKLPEGTKMTKYHVEFLGPLGKELTQAWAVAMALGVEDKVTVPLFEAVQKTQTV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
QSAADIRKVFVDAGVKGEDYDAAWNSFVVKSLVAQQEKAAADLQLQGVPAMFVNGKYQIN
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEECCEEEEC
PQGMDTSSMDVFVQQYADTVKYLVDKK
CCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKIWLALAGMVLAFSASAAQISDGKQYITLDKPVAGEPQVLEFFSFYCPHCYQFEEVLH
CHHHHHHHHHHHHHHCCCHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHH
VSDNVKKKLPEGTKMTKYHVEFLGPLGKELTQAWAVAMALGVEDKVTVPLFEAVQKTQTV
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
QSAADIRKVFVDAGVKGEDYDAAWNSFVVKSLVAQQEKAAADLQLQGVPAMFVNGKYQIN
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEECCEEEEC
PQGMDTSSMDVFVQQYADTVKYLVDKK
CCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA