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The map label for this gene is aroE [C]

Identifier: 16767143

GI number: 16767143

Start: 4066214

End: 4067032

Strand: Reverse

Name: aroE [C]

Synonym: STM3859

Alternate gene names: 16767143

Gene position: 4067032-4066214 (Counterclockwise)

Preceding gene: 16767145

Following gene: 16767142

Centisome position: 83.73

GC content: 52.75

Gene sequence:

>819_bases
ATGACAAAGCGCATTAATAAAGACACTCAGGTCTGCATGTCCCTGGCGGCACGCCCAAGTAACTTCGGTACACGCTTTCA
CAACTATCTGTACGAAGCGCTGGATCTCAATTATCTCTACAAAGCTTTTTCGCCGACCGATCTTACGCAGGCCATTAGCG
GCGTGCGCGGGTTGGGGATCCGTGGATGCGCGATCTCTATGCCGTTTAAAGAAGCCTGCATTCCGCTGGTGGATGAACTG
GATGCTTCCGCTCAGGCCATTCACTCAGTTAATACCATTGTGAATACCAATGGCCATCTGAAGGCGTACAACACCGATTA
CATTGCAATAGCTCGCTTGCTGGCGAATTACAAGGTCTCTCCCGACCTGGTGTTTGCTTTGCGTGGCAGCGGTGGTATGG
CAAAGGCGGTGGCCTGCGCGCTGAAGGATGCGGGGTTTACCCGTGGTTATATTGTCGCGATTGATGAAGCGTCAGGTAAA
CAACTGGCCGAACTCTATGGCTATGAATGGCGCCCGGATACCGACGGTATTGAAGCTGATTTGTTAATTAACGCCACGCC
TATCGGAATGGCTGGCGGTAATGATGCCGACAAACTTTCTTATTCCGAACAGGAAGTGGATAAGGCGAGCGTTATCTTTG
ATGTGGTGGCGCTACCGCCCGTCACGCCGCTAATCCGCTACGCCCGTGAACGCGGTAAAACCGTGATTACCGGTTCCGAA
GTGTTTGCTATTCAGGCCGTGGAGCAATTTGTCCTTTATACCGGGATTCGCCCGGATGACGCGCTGTTCCAGAAAGCAGC
GGCCTGGTCACGCCTGTAA

Upstream 100 bases:

>100_bases
TGTCATAAAAGGTGAAATTTCCCGAACGGGAATGAGCAATTTTTAATATCCCCTTTTTAGCCGATGATTTTCTCTATCGC
AACTTCTATGAGGATTCATC

Downstream 100 bases:

>100_bases
CATTTTTTCCCGCTTTTTCTTTAAATTAAAAGAACAGGGTACCCTACTATGACTAAAAAAATTGTTGCTGTGACGGCCTG
TCCGACGGGCGTCGCCCATA

Product: shikimate 5-dehydrogenase

Products: 3-dehydroshikimate; NADPH; H+

Alternate protein names: SDH-L [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MTKRINKDTQVCMSLAARPSNFGTRFHNYLYEALDLNYLYKAFSPTDLTQAISGVRGLGIRGCAISMPFKEACIPLVDEL
DASAQAIHSVNTIVNTNGHLKAYNTDYIAIARLLANYKVSPDLVFALRGSGGMAKAVACALKDAGFTRGYIVAIDEASGK
QLAELYGYEWRPDTDGIEADLLINATPIGMAGGNDADKLSYSEQEVDKASVIFDVVALPPVTPLIRYARERGKTVITGSE
VFAIQAVEQFVLYTGIRPDDALFQKAAAWSRL

Sequences:

>Translated_272_residues
MTKRINKDTQVCMSLAARPSNFGTRFHNYLYEALDLNYLYKAFSPTDLTQAISGVRGLGIRGCAISMPFKEACIPLVDEL
DASAQAIHSVNTIVNTNGHLKAYNTDYIAIARLLANYKVSPDLVFALRGSGGMAKAVACALKDAGFTRGYIVAIDEASGK
QLAELYGYEWRPDTDGIEADLLINATPIGMAGGNDADKLSYSEQEVDKASVIFDVVALPPVTPLIRYARERGKTVITGSE
VFAIQAVEQFVLYTGIRPDDALFQKAAAWSRL
>Mature_271_residues
TKRINKDTQVCMSLAARPSNFGTRFHNYLYEALDLNYLYKAFSPTDLTQAISGVRGLGIRGCAISMPFKEACIPLVDELD
ASAQAIHSVNTIVNTNGHLKAYNTDYIAIARLLANYKVSPDLVFALRGSGGMAKAVACALKDAGFTRGYIVAIDEASGKQ
LAELYGYEWRPDTDGIEADLLINATPIGMAGGNDADKLSYSEQEVDKASVIFDVVALPPVTPLIRYARERGKTVITGSEV
FAIQAVEQFVLYTGIRPDDALFQKAAAWSRL

Specific function: The physiological substrate is not known. Has much lower activity towards shikimate than AroE [H]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787983, Length=259, Percent_Identity=27.7992277992278, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789675, Length=246, Percent_Identity=25.609756097561, Blast_Score=65, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320332, Length=263, Percent_Identity=26.2357414448669, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR013708
- InterPro:   IPR006151 [H]

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N [H]

EC number: 1.1.1.25

Molecular weight: Translated: 29572; Mature: 29441

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRINKDTQVCMSLAARPSNFGTRFHNYLYEALDLNYLYKAFSPTDLTQAISGVRGLGI
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
RGCAISMPFKEACIPLVDELDASAQAIHSVNTIVNTNGHLKAYNTDYIAIARLLANYKVS
CCEEEECCHHHHHHHHHHHCCHHHHHHHHHHHEECCCCCEEEECCCHHHHHHHHHCCCCC
PDLVFALRGSGGMAKAVACALKDAGFTRGYIVAIDEASGKQLAELYGYEWRPDTDGIEAD
CCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHCCCCCCCCCCCCCEE
LLINATPIGMAGGNDADKLSYSEQEVDKASVIFDVVALPPVTPLIRYARERGKTVITGSE
EEEECCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCH
VFAIQAVEQFVLYTGIRPDDALFQKAAAWSRL
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TKRINKDTQVCMSLAARPSNFGTRFHNYLYEALDLNYLYKAFSPTDLTQAISGVRGLGI
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
RGCAISMPFKEACIPLVDELDASAQAIHSVNTIVNTNGHLKAYNTDYIAIARLLANYKVS
CCEEEECCHHHHHHHHHHHCCHHHHHHHHHHHEECCCCCEEEECCCHHHHHHHHHCCCCC
PDLVFALRGSGGMAKAVACALKDAGFTRGYIVAIDEASGKQLAELYGYEWRPDTDGIEAD
CCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHCCCCCCCCCCCCCEE
LLINATPIGMAGGNDADKLSYSEQEVDKASVIFDVVALPPVTPLIRYARERGKTVITGSE
EEEECCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCH
VFAIQAVEQFVLYTGIRPDDALFQKAAAWSRL
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: shikimate; NADP+

Specific reaction: shikimate + NADP+ = 3-dehydroshikimate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]