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The map label for this gene is lpfB

Identifier: 16766925

GI number: 16766925

Start: 3826666

End: 3827364

Strand: Reverse

Name: lpfB

Synonym: STM3639

Alternate gene names: 16766925

Gene position: 3827364-3826666 (Counterclockwise)

Preceding gene: 16766926

Following gene: 16766924

Centisome position: 78.79

GC content: 44.78

Gene sequence:

>699_bases
ATGAACCGCTCACGTTTGATATCTTGCACAGCACTGGTGCTGGCGTTGATTGCTCAAAACAGTTTTGCCGGAGGCGTGGC
ATTAAGCAGCACGCGTGTTATTTATGACGGTAGTAGAAAGGAAGCTTCTCTTACGGTAAATAATAAAAGCACCACGGATG
AATTTCTCATTCAGTCATGGATTGATGATGCTAACGGTAATAAAAAGACGCCCTTTATCATCACTCCACCGTTATTTAAA
TTAAGCCCGACTAAAAATAACGTTTTACGTATTGTTAATACGACGAACACGTTACCGCAGGATCGCGAGTCCGTTTATTG
GATTAACGTAAAAGCTATTCCTGCCAAAAGTGAAGACGCGGAAGCTAAAAACGTACTGCAGATCGCCGTACGTACCCGCT
TAAAACTGTTCTATCGCCCGGCGGGCCTGAAAGGCAATAGCATGGACGGCTGGAACAAACTGCAGTTCACCAGCGCAGGG
GCTAACCAGATCAAAGTGGAAAACCCATCTGCCTTTAACCTGACGTTTAATAAATTTTATGCCAACGGCCGTGATATTGA
AAAAACGGGAATGGTTCCGGCAAAAGGCTCATTGAATATTGAACTGCCAGCCGGCACCGGCAAGGTAAGCGAAGTTAAAT
ACAACATTATTAATGACTTTGGCACTGCTGGCGACATGTTGACACAGCGCGTTAACTAA

Upstream 100 bases:

>100_bases
CCTGTCCTACGAATAATCGAATTTTCGTTAATACAGACAATCATAATGGCAACGGAAATCCCGTTGCCATTTTTTCCAGC
GGAGGCTCAGGAAGAGAATC

Downstream 100 bases:

>100_bases
CACGTTTTAAAGGATTATTACTATGACATGGACGCATCTTCCTCTGGGCAATAAGACCTCGCGTTTCACGCAGTCTGCGC
TTGCGCTGATGATAGCGGGT

Product: long polar fimbrial chaperone precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MNRSRLISCTALVLALIAQNSFAGGVALSSTRVIYDGSRKEASLTVNNKSTTDEFLIQSWIDDANGNKKTPFIITPPLFK
LSPTKNNVLRIVNTTNTLPQDRESVYWINVKAIPAKSEDAEAKNVLQIAVRTRLKLFYRPAGLKGNSMDGWNKLQFTSAG
ANQIKVENPSAFNLTFNKFYANGRDIEKTGMVPAKGSLNIELPAGTGKVSEVKYNIINDFGTAGDMLTQRVN

Sequences:

>Translated_232_residues
MNRSRLISCTALVLALIAQNSFAGGVALSSTRVIYDGSRKEASLTVNNKSTTDEFLIQSWIDDANGNKKTPFIITPPLFK
LSPTKNNVLRIVNTTNTLPQDRESVYWINVKAIPAKSEDAEAKNVLQIAVRTRLKLFYRPAGLKGNSMDGWNKLQFTSAG
ANQIKVENPSAFNLTFNKFYANGRDIEKTGMVPAKGSLNIELPAGTGKVSEVKYNIINDFGTAGDMLTQRVN
>Mature_232_residues
MNRSRLISCTALVLALIAQNSFAGGVALSSTRVIYDGSRKEASLTVNNKSTTDEFLIQSWIDDANGNKKTPFIITPPLFK
LSPTKNNVLRIVNTTNTLPQDRESVYWINVKAIPAKSEDAEAKNVLQIAVRTRLKLFYRPAGLKGNSMDGWNKLQFTSAG
ANQIKVENPSAFNLTFNKFYANGRDIEKTGMVPAKGSLNIELPAGTGKVSEVKYNIINDFGTAGDMLTQRVN

Specific function: Required for the biogenesis of long polar fimbria; binds and interact with lpfA

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain

Homologues:

Organism=Escherichia coli, GI1789532, Length=224, Percent_Identity=39.7321428571429, Blast_Score=170, Evalue=8e-44,
Organism=Escherichia coli, GI1790771, Length=217, Percent_Identity=40.0921658986175, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI1786743, Length=223, Percent_Identity=36.7713004484305, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1787171, Length=216, Percent_Identity=36.1111111111111, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1786333, Length=242, Percent_Identity=30.9917355371901, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1786936, Length=198, Percent_Identity=29.7979797979798, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1788677, Length=209, Percent_Identity=32.5358851674641, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI87082203, Length=209, Percent_Identity=30.1435406698565, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788428, Length=182, Percent_Identity=32.4175824175824, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87081806, Length=231, Percent_Identity=25.974025974026, Blast_Score=80, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPFB_SALTY (P43661)

Other databases:

- EMBL:   U18559
- EMBL:   AE006468
- PIR:   B56271
- RefSeq:   NP_462540.1
- ProteinModelPortal:   P43661
- SMR:   P43661
- GeneID:   1255163
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM3639
- HOGENOM:   HBG758617
- OMA:   INDYGGQ
- ProtClustDB:   PRK15208
- BioCyc:   STYP99287:STM3639-MONOMER
- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147
- Gene3D:   G3DSA:2.60.40.360
- Gene3D:   G3DSA:2.60.40.1070
- PRINTS:   PR00969

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N; SSF49354 PapD-like; SSF49584 Pili_assmbl_chaperone_C_bac

EC number: NA

Molecular weight: Translated: 25461; Mature: 25461

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSRLISCTALVLALIAQNSFAGGVALSSTRVIYDGSRKEASLTVNNKSTTDEFLIQSW
CCCHHHHHHHHHHHHHHHCCCCCCCEEECCEEEEEECCCCEEEEEECCCCCHHHHHHHHH
IDDANGNKKTPFIITPPLFKLSPTKNNVLRIVNTTNTLPQDRESVYWINVKAIPAKSEDA
HHCCCCCCCCCEEECCCCEEECCCCCCEEEEEECCCCCCCCCCCEEEEEEEEECCCCCCC
EAKNVLQIAVRTRLKLFYRPAGLKGNSMDGWNKLQFTSAGANQIKVENPSAFNLTFNKFY
HHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCEEEEECCCCCEEEECCCCEEEEEEHEEE
ANGRDIEKTGMVPAKGSLNIELPAGTGKVSEVKYNIINDFGTAGDMLTQRVN
CCCCCCHHHCCCCCCCCEEEEECCCCCCCCEEEEEEHHCCCCHHHHHHHCCC
>Mature Secondary Structure
MNRSRLISCTALVLALIAQNSFAGGVALSSTRVIYDGSRKEASLTVNNKSTTDEFLIQSW
CCCHHHHHHHHHHHHHHHCCCCCCCEEECCEEEEEECCCCEEEEEECCCCCHHHHHHHHH
IDDANGNKKTPFIITPPLFKLSPTKNNVLRIVNTTNTLPQDRESVYWINVKAIPAKSEDA
HHCCCCCCCCCEEECCCCEEECCCCCCEEEEEECCCCCCCCCCCEEEEEEEEECCCCCCC
EAKNVLQIAVRTRLKLFYRPAGLKGNSMDGWNKLQFTSAGANQIKVENPSAFNLTFNKFY
HHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCEEEEECCCCCEEEECCCCEEEEEEHEEE
ANGRDIEKTGMVPAKGSLNIELPAGTGKVSEVKYNIINDFGTAGDMLTQRVN
CCCCCCHHHCCCCCCCCEEEEECCCCCCCCEEEEEEHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7721701; 11677609