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The map label for this gene is nusA

Identifier: 16766585

GI number: 16766585

Start: 3456313

End: 3457815

Strand: Reverse

Name: nusA

Synonym: STM3287

Alternate gene names: 16766585

Gene position: 3457815-3456313 (Counterclockwise)

Preceding gene: 161353583

Following gene: 16766584

Centisome position: 71.19

GC content: 52.56

Gene sequence:

>1503_bases
ATGAACAAAGAAATTTTGGCTGTTGTTGAAGCCGTCTCCAACGAAAAAGCGCTGCCACGCGAAAAAATTTTTGAAGCGCT
GGAAAGTGCGCTGGCGACAGCAACAAAGAAAAAATATGAGCAGGAGATCGATGTTCGTGTAGAAATCGATCGTAAAAGCG
GTGATTTTGATACTTTCCGCCGTTGGTTGATCGTTGAAGAAGTGACCATGCCGACGAAGGAAATTACGCTGGAAGCGGCG
CGTTTTGAAGACGAAAGCCTGAATGTCGGCGACTATGTTGAAGATCAGATTGAATCTGTCACCTTTGACCGTATCACCAC
GCAGACTGCGAAACAGGTTATCGTGCAGAAGGTCCGTGAAGCTGAACGGGCGATGGTTGTCGATCAGTTCCGCGACCAGG
AAGGCGAAATTGTCACTGGCGTGGTGAAGAAAGTGAACCGCGACAATATCTCTCTGGAAATTAAATCCGAAGGGATGGCC
GGTAACGCTGAAGCGGTGATTCTGCGTGAAGATATGCTGCCGCGCGAAAACTTCCGCCCTGGCGACCGTATTCGCGGCGT
GCTGTATGCTGTTCGACCGGAAGCGCGTGGCGCGCAGCTGTTCGTCACCCGTTCCAAGCCGGAAATGCTGATCGAACTGT
TCCGCATCGAAGTGCCGGAAATCGGCGAAGAAGTGATTGAAATTAAAGCGGCGGCTCGCGATCCGGGTTCTCGTGCGAAA
ATCGCAGTGAAAACCAACGATAAACGTATCGATCCGGTCGGCGCTTGCGTGGGGATGCGCGGCGCGCGCGTTCAGGCGGT
CTCTACCGAACTGGGCGGTGAGCGTATTGATATCGTGCTGTGGGATGATAACCCGGCGCAGTTCGTCATTAATGCGATGG
CGCCGGCAGACGTCGCGTCTATCGTGGTGGACGAAGATAAACATACCATGGATATCGCCGTTGAAGCCGGTAATCTGGCG
CAGGCGATCGGACGTAATGGTCAGAACGTCCGCCTGGCTTCGCAATTGAGCGGCTGGGAACTCAACGTAATGACCGTTGA
TGACTTGCAGGCTAAACATCAGGCTGAAGCACATGCCGCTATCGAGATTTTTACTAAATATCTTGATATTGATGAAGAGT
TCGCGACCGTTCTGGTAGAAGAAGGTTTCTCCACGCTCGAGGAACTGGCCTATGTGCCAATGAAAGAACTGCTGGAAATT
GACGGCCTTGATGAGCCGACCGTTGAAGCACTGCGCGAGCGTGCTAAAAACGCACTGGCCACTCTGGCGCAGGACCAGGA
AGCAAGCCTCGGTGATAACAAACCGGCTGACGATCTGCTGAATCTGGAAGGATTAGATCGCGATATGGCTTTCAAACTGG
CGGCTCGTGGTGTTTGTACGCTGGAAGATCTCGCCGACCAGGGCATTGATGATCTGGCTGATATCGAAGGGTTGACCGAC
GAAAAAGCCGGTGAGCTGATTATGGCTGCCCGTAATATTTGCTGGTTCGGCGACGAAGCGTAA

Upstream 100 bases:

>100_bases
CACAGTCGAAGGCAAAGATGAAGTGTTCGCGCTGAGTAATATCCAGAAGGCGAACCTGGTTCCCCACTTTTAACAGTCTG
GATGAGGTGAAAAGCCCGCG

Downstream 100 bases:

>100_bases
TAAACTGTAGCAGGAAGGAACAGCATGACAGATGTAACCCTAAAAGCGCTGGCCGCAGAGAGACAGGTTTCCGTGGATCG
CCTGGTACAGCAGTTTGCTG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: L factor; N utilization substance protein A

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVRVEIDRKSGDFDTFRRWLIVEEVTMPTKEITLEAA
RFEDESLNVGDYVEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVDQFRDQEGEIVTGVVKKVNRDNISLEIKSEGMA
GNAEAVILREDMLPRENFRPGDRIRGVLYAVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAK
IAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLA
QAIGRNGQNVRLASQLSGWELNVMTVDDLQAKHQAEAHAAIEIFTKYLDIDEEFATVLVEEGFSTLEELAYVPMKELLEI
DGLDEPTVEALRERAKNALATLAQDQEASLGDNKPADDLLNLEGLDRDMAFKLAARGVCTLEDLADQGIDDLADIEGLTD
EKAGELIMAARNICWFGDEA

Sequences:

>Translated_500_residues
MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVRVEIDRKSGDFDTFRRWLIVEEVTMPTKEITLEAA
RFEDESLNVGDYVEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVDQFRDQEGEIVTGVVKKVNRDNISLEIKSEGMA
GNAEAVILREDMLPRENFRPGDRIRGVLYAVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAK
IAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLA
QAIGRNGQNVRLASQLSGWELNVMTVDDLQAKHQAEAHAAIEIFTKYLDIDEEFATVLVEEGFSTLEELAYVPMKELLEI
DGLDEPTVEALRERAKNALATLAQDQEASLGDNKPADDLLNLEGLDRDMAFKLAARGVCTLEDLADQGIDDLADIEGLTD
EKAGELIMAARNICWFGDEA
>Mature_500_residues
MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVRVEIDRKSGDFDTFRRWLIVEEVTMPTKEITLEAA
RFEDESLNVGDYVEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVDQFRDQEGEIVTGVVKKVNRDNISLEIKSEGMA
GNAEAVILREDMLPRENFRPGDRIRGVLYAVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAK
IAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLA
QAIGRNGQNVRLASQLSGWELNVMTVDDLQAKHQAEAHAAIEIFTKYLDIDEEFATVLVEEGFSTLEELAYVPMKELLEI
DGLDEPTVEALRERAKNALATLAQDQEASLGDNKPADDLLNLEGLDRDMAFKLAARGVCTLEDLADQGIDDLADIEGLTD
EKAGELIMAARNICWFGDEA

Specific function: Participates in both the termination and antitermination of transcription. NusA binds directly to the core enzyme of the DNA-dependent RNA polymerase. NusA also interacts with lambda N protein, RNA, rho factor, and perhaps nusB. Binds RNA

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Escherichia coli, GI1789560, Length=500, Percent_Identity=93.8, Blast_Score=946, Evalue=0.0,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NUSA_SALTY (P37430)

Other databases:

- EMBL:   M61008
- EMBL:   AE006468
- PIR:   A53377
- RefSeq:   NP_462200.1
- ProteinModelPortal:   P37430
- PRIDE:   P37430
- GeneID:   1254810
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM3287
- HOGENOM:   HBG529269
- OMA:   RSKISVY
- ProtClustDB:   PRK09202
- BioCyc:   STYP99287:STM3287-MONOMER
- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213
- Gene3D:   G3DSA:3.30.300.20
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.30.1480.10
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR01953
- TIGRFAMs:   TIGR01954

Pfam domain/function: PF08529 NusA_N; PF00575 S1; SSF54814 KH_prok; SSF50249 Nucleic_acid_OB; SSF47794 Rad51_N; SSF69705 TF_NusA_N

EC number: NA

Molecular weight: Translated: 55426; Mature: 55426

Theoretical pI: Translated: 4.22; Mature: 4.22

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVRVEIDRKSGDFDTFR
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHH
RWLIVEEVTMPTKEITLEAARFEDESLNVGDYVEDQIESVTFDRITTQTAKQVIVQKVRE
HHHEEHHHCCCHHHHEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERAMVVDQFRDQEGEIVTGVVKKVNRDNISLEIKSEGMAGNAEAVILREDMLPRENFRP
HHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCC
GDRIRGVLYAVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAK
CHHHCEEEEEECCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCE
IAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQFVINAMAPADVAS
EEEECCCCCCCHHHHHHCCCCCEEEEEEHHCCCCEEEEEEECCCCHHEEEECCCCCCCEE
IVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMTVDDLQAKHQAEAHAA
EEECCCCCEEEEEEECCHHHHHHCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHHH
IEIFTKYLDIDEEFATVLVEEGFSTLEELAYVPMKELLEIDGLDEPTVEALRERAKNALA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHH
TLAQDQEASLGDNKPADDLLNLEGLDRDMAFKLAARGVCTLEDLADQGIDDLADIEGLTD
HHHCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHCCCHHHHHHCCCCC
EKAGELIMAARNICWFGDEA
CHHCHHHHHHCCEEECCCCC
>Mature Secondary Structure
MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVRVEIDRKSGDFDTFR
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHH
RWLIVEEVTMPTKEITLEAARFEDESLNVGDYVEDQIESVTFDRITTQTAKQVIVQKVRE
HHHEEHHHCCCHHHHEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERAMVVDQFRDQEGEIVTGVVKKVNRDNISLEIKSEGMAGNAEAVILREDMLPRENFRP
HHHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCC
GDRIRGVLYAVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAK
CHHHCEEEEEECCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCE
IAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQFVINAMAPADVAS
EEEECCCCCCCHHHHHHCCCCCEEEEEEHHCCCCEEEEEEECCCCHHEEEECCCCCCCEE
IVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMTVDDLQAKHQAEAHAA
EEECCCCCEEEEEEECCHHHHHHCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHHH
IEIFTKYLDIDEEFATVLVEEGFSTLEELAYVPMKELLEIDGLDEPTVEALRERAKNALA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHH
TLAQDQEASLGDNKPADDLLNLEGLDRDMAFKLAARGVCTLEDLADQGIDDLADIEGLTD
HHHCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHCCCHHHHHHCCCCC
EKAGELIMAARNICWFGDEA
CHHCHHHHHHCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113180; 11677609