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The map label for this gene is budR [H]

Identifier: 16766421

GI number: 16766421

Start: 3279660

End: 3280538

Strand: Direct

Name: budR [H]

Synonym: STM3121

Alternate gene names: 16766421

Gene position: 3279660-3280538 (Clockwise)

Preceding gene: 16766416

Following gene: 16766424

Centisome position: 67.52

GC content: 52.45

Gene sequence:

>879_bases
ATGGAACTGCGCCATATTCGCTATTTTCTCGCCGTCGCTGAAGAGCGGCATTTCACGCGGGCTGCGACAAAATTAGGCAT
AGGTCAGCCGCCGCTAAGCCAACAGATTAAGGATCTGGAAAGAGAGCTGGGAGCGCAGCTATTCCGGAGAGTGCCTCATG
GCGCGGAATTAACCGAGGCTGGAAAAGCATTTTATGATGTTGTTAAAGGGATGCCTGCTACCGCGACAAGAGCCGTGCTG
GCGGCGCAGCGTGTCGCCAGGGGGGAGTCGGGCGTGTTAAGGGTGGGTTTTACTGCCTCTGCGGCATTTAACAGTGTGGT
GCCCGGCGCAATCCGTACTTTCAAGCGGGCTTATCCTGACGTTCGTTTGCAGCTTGAGGAAGGAAATACAACCCAACTGG
CTGATGAATTGAATGAAGGATCGCTGGATGTTGCCTTTCTGAGACCCGGTTTTACAGGCAATGAACGGTTCCAGCTCAGG
TTACTGTCTGAAGAGCCAATGGTTATTGTGCTGGCAGAAACGCATCCTGCTGCGGCGTGTAAACAGATTGCACTCTCGAT
TCTCAAAGATGAGTTTTTTTTACTCTTCCCACGTGAAATCGGCCTGTCGCTTTATGACGCAGTGATCAAGGCCTGCGGTA
AAGCGGGCTTTGAACCGAAAATTGGTCAACTCGTGCCGCAAATTTCGTCAGTCATTAACCTTGTGTCGGCAGAGATGGGC
GTGTCGATGGTTCCAGATTCAATGAGACAGGTTAATGTCAAGGGAGTAGTATACCGCCCCGTTGCAGACCAGATGCCCGT
CGCAAAACTGGCTCTGGCATACCGGCGCGGCGATACATCGCCAACGCTGAGAAACTTCATCCTGAAAGTCACTGGCTGA

Upstream 100 bases:

>100_bases
ATCGGGCATATTGCACCTCCTGTTAACCTCACTATGCGCTCGCTGGTTTCATTCTTCCAATATATGATGGTGCCATTCTT
AATTGCTTTTGGAGATGAGG

Downstream 100 bases:

>100_bases
ATGCGGTTATGTGAGCGTTGAATAATCATTTGAAACGTAACCAATAATTATTGGTCCGATTTTGTGTCTAAAGGAATCGC
TAACGGAGCAGGAACTGGCT

Product: transcriptional regulator

Products: NA

Alternate protein names: Bud operon transcriptional regulator [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MELRHIRYFLAVAEERHFTRAATKLGIGQPPLSQQIKDLERELGAQLFRRVPHGAELTEAGKAFYDVVKGMPATATRAVL
AAQRVARGESGVLRVGFTASAAFNSVVPGAIRTFKRAYPDVRLQLEEGNTTQLADELNEGSLDVAFLRPGFTGNERFQLR
LLSEEPMVIVLAETHPAAACKQIALSILKDEFFLLFPREIGLSLYDAVIKACGKAGFEPKIGQLVPQISSVINLVSAEMG
VSMVPDSMRQVNVKGVVYRPVADQMPVAKLALAYRRGDTSPTLRNFILKVTG

Sequences:

>Translated_292_residues
MELRHIRYFLAVAEERHFTRAATKLGIGQPPLSQQIKDLERELGAQLFRRVPHGAELTEAGKAFYDVVKGMPATATRAVL
AAQRVARGESGVLRVGFTASAAFNSVVPGAIRTFKRAYPDVRLQLEEGNTTQLADELNEGSLDVAFLRPGFTGNERFQLR
LLSEEPMVIVLAETHPAAACKQIALSILKDEFFLLFPREIGLSLYDAVIKACGKAGFEPKIGQLVPQISSVINLVSAEMG
VSMVPDSMRQVNVKGVVYRPVADQMPVAKLALAYRRGDTSPTLRNFILKVTG
>Mature_292_residues
MELRHIRYFLAVAEERHFTRAATKLGIGQPPLSQQIKDLERELGAQLFRRVPHGAELTEAGKAFYDVVKGMPATATRAVL
AAQRVARGESGVLRVGFTASAAFNSVVPGAIRTFKRAYPDVRLQLEEGNTTQLADELNEGSLDVAFLRPGFTGNERFQLR
LLSEEPMVIVLAETHPAAACKQIALSILKDEFFLLFPREIGLSLYDAVIKACGKAGFEPKIGQLVPQISSVINLVSAEMG
VSMVPDSMRQVNVKGVVYRPVADQMPVAKLALAYRRGDTSPTLRNFILKVTG

Specific function: Regulator of the budABC operon for 2,3-butanediol synthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=287, Percent_Identity=38.6759581881533, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1788887, Length=287, Percent_Identity=35.8885017421603, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI2367136, Length=290, Percent_Identity=30.6896551724138, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI157672245, Length=247, Percent_Identity=35.2226720647773, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1790399, Length=283, Percent_Identity=31.095406360424, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787601, Length=281, Percent_Identity=28.1138790035587, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788297, Length=147, Percent_Identity=31.2925170068027, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788748, Length=245, Percent_Identity=23.265306122449, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1789173, Length=138, Percent_Identity=37.6811594202899, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787530, Length=223, Percent_Identity=26.457399103139, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787589, Length=141, Percent_Identity=31.9148936170213, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081904, Length=183, Percent_Identity=30.6010928961749, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI145693105, Length=179, Percent_Identity=26.2569832402235, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790262, Length=250, Percent_Identity=24.8, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082132, Length=298, Percent_Identity=25.503355704698, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790208, Length=146, Percent_Identity=29.4520547945205, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788481, Length=178, Percent_Identity=25.2808988764045, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31964; Mature: 31964

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRHIRYFLAVAEERHFTRAATKLGIGQPPLSQQIKDLERELGAQLFRRVPHGAELTEA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
GKAFYDVVKGMPATATRAVLAAQRVARGESGVLRVGFTASAAFNSVVPGAIRTFKRAYPD
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHCCCHHHHHHHHCCCC
VRLQLEEGNTTQLADELNEGSLDVAFLRPGFTGNERFQLRLLSEEPMVIVLAETHPAAAC
EEEEECCCCCHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEECCCCEEEEEECCCCHHHH
KQIALSILKDEFFLLFPREIGLSLYDAVIKACGKAGFEPKIGQLVPQISSVINLVSAEMG
HHHHHHHHHCCCEEEECHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
VSMVPDSMRQVNVKGVVYRPVADQMPVAKLALAYRRGDTSPTLRNFILKVTG
CCCCCCHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHEEEECC
>Mature Secondary Structure
MELRHIRYFLAVAEERHFTRAATKLGIGQPPLSQQIKDLERELGAQLFRRVPHGAELTEA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
GKAFYDVVKGMPATATRAVLAAQRVARGESGVLRVGFTASAAFNSVVPGAIRTFKRAYPD
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHCCCHHHHHHHHCCCC
VRLQLEEGNTTQLADELNEGSLDVAFLRPGFTGNERFQLRLLSEEPMVIVLAETHPAAAC
EEEEECCCCCHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEECCCCEEEEEECCCCHHHH
KQIALSILKDEFFLLFPREIGLSLYDAVIKACGKAGFEPKIGQLVPQISSVINLVSAEMG
HHHHHHHHHCCCEEEECHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
VSMVPDSMRQVNVKGVVYRPVADQMPVAKLALAYRRGDTSPTLRNFILKVTG
CCCCCCHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA