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The map label for this gene is srlE [H]

Identifier: 16766064

GI number: 16766064

Start: 2890110

End: 2891090

Strand: Direct

Name: srlE [H]

Synonym: STM2752

Alternate gene names: 16766064

Gene position: 2890110-2891090 (Clockwise)

Preceding gene: 16766063

Following gene: 16766065

Centisome position: 59.5

GC content: 62.79

Gene sequence:

>981_bases
ATGAACACGGTACTTATTCCTCCCGGTCCCGGCGGCTACGGTAAAGGGCTTTGGCTGCCCGTTGCCAGCGGCAAAAAGGT
GCTGTCACTGACCGGGCGCGAGATTCATCCGGTGGCGATAGAAATCGGTGCGCTCACTGAATCGGAAGTGGTGAACGGCT
TCTCCGATATCCCGCCTGATAACGACATCCTGTGCGTGGTGATTAACTGCGCCGGGTCCCTGCGCTGCGGCCTGTATCCG
CAGAAGGGCATCCCGACCATCAATGTGCTGCCCACCTGGCGCGCGGGGCCGCTGGCGCAGTTTATTAGCGAGGATAACTA
CGTCTCCGGCGTGACGATAGAGCAACTGGTTCTGGTGGATACGGCCGAAGCGCCGGACGGTGAACCGGAGCCAGTGTCTG
TCACGGCACCACGGATTATCACCACTCCGCCGCCTGCACGTGGCGCGGAAAAACTGGTTCGCATGGTCGAGCGTACCGGC
ACCGCCGTCGGACACGTTATCGCCCTGCTGTTTGCCGCCAGCCGTGAGGCGGTGGACGTGTCGCTGCGCAACGTTATCCC
GTTTATGGCCTTTGTCTCACTGCTGATTGCCCTGGTGCAGGAAACCGCACTCGGCAGCCTGATTGCCCACGCCCTGACGC
CGCTGGCGAACTCGCTGTGGGGACTGGTGCTGCTGTCGCTCATCTGCGGCATTCCGTTTCTCTCCCCGGTGCTCGGGCCG
GGGGCAGCCATCTCGCAGGTCATCGGCGTGATGATTGGCACCCAGATTGGCGCGGGCGCGATATCCCCGGCCTTCGCCCT
GCCCGCGCTGTTCGCCATCAACGTGCAGGTCGGCTGCGACTTCGTGCCGGTAGGTCTTTCGATGCAGGAGGCGAAGCCGG
AGACCATCGCGAAGGGCGTCCCTGCCTTTCTGCTCTCACGTCAGTTAACGGGGCCGCTGGCGGTCATCATCGGCTGGCTG
TTTTCCCTGGGCTTATTCTGA

Upstream 100 bases:

>100_bases
GTGACCTGAAGCCCTGCCACACCGGGGCGATTCATCTCTCCGGCCCGGTGCCGAAACTCCATGACCTGCACGGCAATCTG
GTGATTGAGGAGGGACGGCC

Downstream 100 bases:

>100_bases
GGACATCATCATGAACAAAATCAGAGCCGCCGTGGTGGGTGCAGGTATCTACGGCAAACACCATATGAACGCGTACCGCC
ATAACCCGGACACCGTGCTG

Product: glucitol-specific PTS enzyme III

Products: NA

Alternate protein names: EII-Gut; PTS system glucitol/sorbitol-specific EIIB component [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MNTVLIPPGPGGYGKGLWLPVASGKKVLSLTGREIHPVAIEIGALTESEVVNGFSDIPPDNDILCVVINCAGSLRCGLYP
QKGIPTINVLPTWRAGPLAQFISEDNYVSGVTIEQLVLVDTAEAPDGEPEPVSVTAPRIITTPPPARGAEKLVRMVERTG
TAVGHVIALLFAASREAVDVSLRNVIPFMAFVSLLIALVQETALGSLIAHALTPLANSLWGLVLLSLICGIPFLSPVLGP
GAAISQVIGVMIGTQIGAGAISPAFALPALFAINVQVGCDFVPVGLSMQEAKPETIAKGVPAFLLSRQLTGPLAVIIGWL
FSLGLF

Sequences:

>Translated_326_residues
MNTVLIPPGPGGYGKGLWLPVASGKKVLSLTGREIHPVAIEIGALTESEVVNGFSDIPPDNDILCVVINCAGSLRCGLYP
QKGIPTINVLPTWRAGPLAQFISEDNYVSGVTIEQLVLVDTAEAPDGEPEPVSVTAPRIITTPPPARGAEKLVRMVERTG
TAVGHVIALLFAASREAVDVSLRNVIPFMAFVSLLIALVQETALGSLIAHALTPLANSLWGLVLLSLICGIPFLSPVLGP
GAAISQVIGVMIGTQIGAGAISPAFALPALFAINVQVGCDFVPVGLSMQEAKPETIAKGVPAFLLSRQLTGPLAVIIGWL
FSLGLF
>Mature_326_residues
MNTVLIPPGPGGYGKGLWLPVASGKKVLSLTGREIHPVAIEIGALTESEVVNGFSDIPPDNDILCVVINCAGSLRCGLYP
QKGIPTINVLPTWRAGPLAQFISEDNYVSGVTIEQLVLVDTAEAPDGEPEPVSVTAPRIITTPPPARGAEKLVRMVERTG
TAVGHVIALLFAASREAVDVSLRNVIPFMAFVSLLIALVQETALGSLIAHALTPLANSLWGLVLLSLICGIPFLSPVLGP
GAAISQVIGVMIGTQIGAGAISPAFALPALFAINVQVGCDFVPVGLSMQEAKPETIAKGVPAFLLSRQLTGPLAVIIGWL
FSLGLF

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3732

COG function: function code G; Phosphotransferase system sorbitol-specific component IIBC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-5 domain [H]

Homologues:

Organism=Escherichia coli, GI48994905, Length=323, Percent_Identity=45.5108359133127, Blast_Score=266, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011638
- InterPro:   IPR011618
- InterPro:   IPR004702 [H]

Pfam domain/function: PF07663 EIIBC-GUT_C; PF03612 EIIBC-GUT_N [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 33853; Mature: 33853

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS51102 PTS_EIIB_TYPE_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTVLIPPGPGGYGKGLWLPVASGKKVLSLTGREIHPVAIEIGALTESEVVNGFSDIPPD
CCEEEECCCCCCCCCCEEEECCCCCEEEEECCCEECEEEEEECCCCHHHHHCCCCCCCCC
NDILCVVINCAGSLRCGLYPQKGIPTINVLPTWRAGPLAQFISEDNYVSGVTIEQLVLVD
CCEEEEEEECCCCCEECCCCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCCCHHHEEEEE
TAEAPDGEPEPVSVTAPRIITTPPPARGAEKLVRMVERTGTAVGHVIALLFAASREAVDV
CCCCCCCCCCCEEEECCEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHH
SLRNVIPFMAFVSLLIALVQETALGSLIAHALTPLANSLWGLVLLSLICGIPFLSPVLGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCC
GAAISQVIGVMIGTQIGAGAISPAFALPALFAINVQVGCDFVPVGLSMQEAKPETIAKGV
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEEEEECCCEEECCCCHHHCCCHHHHHCC
PAFLLSRQLTGPLAVIIGWLFSLGLF
HHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNTVLIPPGPGGYGKGLWLPVASGKKVLSLTGREIHPVAIEIGALTESEVVNGFSDIPPD
CCEEEECCCCCCCCCCEEEECCCCCEEEEECCCEECEEEEEECCCCHHHHHCCCCCCCCC
NDILCVVINCAGSLRCGLYPQKGIPTINVLPTWRAGPLAQFISEDNYVSGVTIEQLVLVD
CCEEEEEEECCCCCEECCCCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCCCHHHEEEEE
TAEAPDGEPEPVSVTAPRIITTPPPARGAEKLVRMVERTGTAVGHVIALLFAASREAVDV
CCCCCCCCCCCEEEECCEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHH
SLRNVIPFMAFVSLLIALVQETALGSLIAHALTPLANSLWGLVLLSLICGIPFLSPVLGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCC
GAAISQVIGVMIGTQIGAGAISPAFALPALFAINVQVGCDFVPVGLSMQEAKPETIAKGV
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEEEEECCCEEECCCCHHHCCCHHHHHCC
PAFLLSRQLTGPLAVIIGWLFSLGLF
HHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9435786 [H]