| Definition | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 chromosome, complete genome. |
|---|---|
| Accession | NC_003197 |
| Length | 4,857,432 |
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The map label for this gene is ligA [H]
Identifier: 16765747
GI number: 16765747
Start: 2539001
End: 2541016
Strand: Reverse
Name: ligA [H]
Synonym: STM2427
Alternate gene names: 16765747
Gene position: 2541016-2539001 (Counterclockwise)
Preceding gene: 16765748
Following gene: 16765746
Centisome position: 52.31
GC content: 56.89
Gene sequence:
>2016_bases ATGGAACCAATCGAACAACAACTGACAGAACTGCGAACCACGCTTCGCCATCATGAATATCTGTATCATGTGATGGATGC GCCAGAGATTCCCGACGCCGAATATGACCGCCTGATGCGGGAACTGCGAGAGCTGGAGGCGCAACGTCCGGACCTTATCA CGCCGGATTCGCCCACGCAGCGCGTTGGCGCGGCGCCGCTGACGGCATTCAACCAGATTCGCCATGAAGTACCGATGCTG TCGCTCGATAACGTATTTGATGAAGAAAGCTTCCTGGCGTTTAACAAGCGAGTGCAGGATCGTCTAAAAAGCACGGAAAA TGTCATCTGGTGCTGCGAGCTAAAGCTGGATGGTCTGGCGGTCAGCATTCTGTATGAAAACGGCGTGCTGGTGAGCGCTG CGACGCGTGGCGACGGCACCACCGGTGAAGATATCACTTCAAACGTGCGCACGATTCGCGCCATTCCGTTAAAGCTGCAC GGCGATAACATTCCGGCGCGTCTTGAAGTTCGCGGAGAGGTATTTTTACCGCAGGCCGGGTTTGAAAAGATCAATGAAGA CGCGCGCCGCACTGGCGGCAAAGTGTTTGCTAACCCGCGTAACGCGGCGGCGGGTTCGCTGCGTCAGCTCGATCCGCGTA TTACGGCGAAGCGACCGCTAACATTTTTCTGCTACGGCGTCGGTATTCTGGAAGGCGGCGAGTTGCCGGATACGCATCTG GGACGTTTGCTGCAATTTAAAGCGTGGGGGTTACCGGTCAGCGATCGCGTGACGCTGTGCGATTCTCCCCAGGCGGTACT GGATTTTTATCACAACGTCGAAAAAGATCGCCCTACGCTCGGTTTTGATATCGACGGCGTGGTGATTAAGGTCAACTCGC TGGCTTTGCAGGAACTGCTGGGCTTTGTGGCCCGCGCGCCGCGCTGGGCGGTGGCGTTTAAATTTCCTGCTCAGGAGCAG ATGACTTTTGTGCGCGACGTTGAGTTTCAGGTCGGGCGTACCGGGGCGATTACGCCGGTGGCGCGTCTGGAACCGGTTCA GGTGGCTGGTGTGCTGGTCAGCAATGCCACGTTGCATAATGCCGATGAAATTGAGCGACTTGGCTTGCGCATTGGCGATA AGGTGGTTATCCGTCGGGCAGGCGATGTGATCCCTCAGGTGGTCAACGTGGTTCTGTCCGAACGCCCTGAAGAGACGCGT CCGATTGTGTTTCCAACGCACTGTCCGGTGTGCGGATCGGACGTAGAGCGCGTTGAAGGCGAAGCCGTTACCCGTTGTAC CGGCGGGCTGATTTGCGGCGCGCAGCGTAAAGAGTCGCTGAAACATTTTGTGTCGCGCCGGGCGATGGATGTAGACGGGA TGGGCGATAAGATCATCGACCAACTGGTGGAAAGGGAGTATGTCCATACGCCAGCGGATCTGTTCCGTCTGACGGCGGGT AAACTGACCGGCCTGGACCGAATGGGGCCAAAGTCCGCGCAAAATGTTGTTAACGCGCTGGAAAAATCCAAAACGACGAC CTTTGCGCGTTTTCTCTATGCGCTGGGCATCCGTGAAGTGGGTGAAGCGACGGCGGCGGGGCTGGCGGCTTATTTCGGTA CGCTGGAGGCGCTGCAGGCCGCCACCATTGACGAGTTGCAGAAAGTACCTGACGTGGGGATTGTGGTCGCTACGCACGTC TTTAACTTTTTTGCCGAAGAGAGTAATCGTGACGTGATTGGGCAGCTACTGGCGGAAGGGGTTCACTGGCCCGCGCCGGT AGTCATTAATGTGCAAGAGATCGATAGCCCGTTTGCCGGTAAAACCGTGGTACTAACCGGTAGCCTGAGCCAGATGTCTC GCGATGACGCTAAAGCGCGTTTAGTGGCGCTGGGCGCGAAAGTGGCAGGCAGCGTATCGAAGAAAACCGATCTGGTGATT GCCGGTGAAGCGGCGGGCTCCAAACTGGCCAAAGCGCAGGAACTGGGTATTACCGTGATTGATGAAGCGGAAATGATCCG CTTACTGGGTGCCTGA
Upstream 100 bases:
>100_bases TCCGCGAGGTGATGGACGCTAACGCCTGAGGTTAACGCAGGTGTGACATTTTTAACGTAACCCCCGCCTGTCGGGGGTTT TTTATCATTGATGGTGTGAT
Downstream 100 bases:
>100_bases GATGGAAAAAGAGCAGCTGGTTGAAATCGCCAACACCGTGATGCCCTTTGGCAAATATCAGGGACGTCGATTGATCGACC TTCCGGAAGAGTATTTGCTG
Product: NAD-dependent DNA ligase LigA
Products: NA
Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]
Number of amino acids: Translated: 671; Mature: 671
Protein sequence:
>671_residues MEPIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELEAQRPDLITPDSPTQRVGAAPLTAFNQIRHEVPML SLDNVFDEESFLAFNKRVQDRLKSTENVIWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH GDNIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGILEGGELPDTHL GRLLQFKAWGLPVSDRVTLCDSPQAVLDFYHNVEKDRPTLGFDIDGVVIKVNSLALQELLGFVARAPRWAVAFKFPAQEQ MTFVRDVEFQVGRTGAITPVARLEPVQVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEETR PIVFPTHCPVCGSDVERVEGEAVTRCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEREYVHTPADLFRLTAG KLTGLDRMGPKSAQNVVNALEKSKTTTFARFLYALGIREVGEATAAGLAAYFGTLEALQAATIDELQKVPDVGIVVATHV FNFFAEESNRDVIGQLLAEGVHWPAPVVINVQEIDSPFAGKTVVLTGSLSQMSRDDAKARLVALGAKVAGSVSKKTDLVI AGEAAGSKLAKAQELGITVIDEAEMIRLLGA
Sequences:
>Translated_671_residues MEPIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELEAQRPDLITPDSPTQRVGAAPLTAFNQIRHEVPML SLDNVFDEESFLAFNKRVQDRLKSTENVIWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH GDNIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGILEGGELPDTHL GRLLQFKAWGLPVSDRVTLCDSPQAVLDFYHNVEKDRPTLGFDIDGVVIKVNSLALQELLGFVARAPRWAVAFKFPAQEQ MTFVRDVEFQVGRTGAITPVARLEPVQVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEETR PIVFPTHCPVCGSDVERVEGEAVTRCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEREYVHTPADLFRLTAG KLTGLDRMGPKSAQNVVNALEKSKTTTFARFLYALGIREVGEATAAGLAAYFGTLEALQAATIDELQKVPDVGIVVATHV FNFFAEESNRDVIGQLLAEGVHWPAPVVINVQEIDSPFAGKTVVLTGSLSQMSRDDAKARLVALGAKVAGSVSKKTDLVI AGEAAGSKLAKAQELGITVIDEAEMIRLLGA >Mature_671_residues MEPIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELEAQRPDLITPDSPTQRVGAAPLTAFNQIRHEVPML SLDNVFDEESFLAFNKRVQDRLKSTENVIWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH GDNIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGILEGGELPDTHL GRLLQFKAWGLPVSDRVTLCDSPQAVLDFYHNVEKDRPTLGFDIDGVVIKVNSLALQELLGFVARAPRWAVAFKFPAQEQ MTFVRDVEFQVGRTGAITPVARLEPVQVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEETR PIVFPTHCPVCGSDVERVEGEAVTRCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEREYVHTPADLFRLTAG KLTGLDRMGPKSAQNVVNALEKSKTTTFARFLYALGIREVGEATAAGLAAYFGTLEALQAATIDELQKVPDVGIVVATHV FNFFAEESNRDVIGQLLAEGVHWPAPVVINVQEIDSPFAGKTVVLTGSLSQMSRDDAKARLVALGAKVAGSVSKKTDLVI AGEAAGSKLAKAQELGITVIDEAEMIRLLGA
Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam
COG id: COG0272
COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 BRCT domain [H]
Homologues:
Organism=Escherichia coli, GI1788750, Length=671, Percent_Identity=92.8464977645306, Blast_Score=1282, Evalue=0.0, Organism=Escherichia coli, GI87082305, Length=587, Percent_Identity=22.8279386712095, Blast_Score=98, Evalue=2e-21,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001357 - InterPro: IPR018239 - InterPro: IPR004150 - InterPro: IPR001679 - InterPro: IPR013839 - InterPro: IPR013840 - InterPro: IPR003583 - InterPro: IPR012340 - InterPro: IPR016027 - InterPro: IPR010994 - InterPro: IPR004149 [H]
Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]
EC number: =6.5.1.2 [H]
Molecular weight: Translated: 73453; Mature: 73453
Theoretical pI: Translated: 5.47; Mature: 5.47
Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.2 %Cys (Translated Protein) 1.5 %Met (Translated Protein) 2.7 %Cys+Met (Translated Protein) 1.2 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 2.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MEPIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELEAQRPDLITPDSPTQ CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHH RVGAAPLTAFNQIRHEVPMLSLDNVFDEESFLAFNKRVQDRLKSTENVIWCCELKLDGLA HCCCCHHHHHHHHHHHCCCEEHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEE VSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLHGDNIPARLEVRGEVFLPQAG EEEEEECCEEEEEECCCCCCCCHHHHHCCEEEEEEEEEEECCCCCEEEEECCEEECCCCC FEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGILEGGELPDTHL HHHHHHHHHHCCCEEEECCCCCCCCCHHHCCCCCCCCCCCEEEEEEEEEECCCCCCHHHH GRLLQFKAWGLPVSDRVTLCDSPQAVLDFYHNVEKDRPTLGFDIDGVVIKVNSLALQELL HHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHH GFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRTGAITPVARLEPVQVAGVLVSNATLHN HHHHCCCCEEEEEECCCHHHHHHHHHCHHCCCCCCCCCCHHHCCCHHEEEEEEECCCCCC ADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEETRPIVFPTHCPVCGSDVERVEG HHHHHHHHHCCCCHHEEHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHHCC EAVTRCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEREYVHTPADLFRLTAG CHHHHHCCCEEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH KLTGLDRMGPKSAQNVVNALEKSKTTTFARFLYALGIREVGEATAAGLAAYFGTLEALQA HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH ATIDELQKVPDVGIVVATHVFNFFAEESNRDVIGQLLAEGVHWPAPVVINVQEIDSPFAG HHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEHHHCCCCCCC KTVVLTGSLSQMSRDDAKARLVALGAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGITVI CEEEEECCHHHHHHHHHHHHHHEECHHHHCCCCCCCCEEEECCCCCCHHHHHHHCCEEEE DEAEMIRLLGA CHHHHHHHHCC >Mature Secondary Structure MEPIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELEAQRPDLITPDSPTQ CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHH RVGAAPLTAFNQIRHEVPMLSLDNVFDEESFLAFNKRVQDRLKSTENVIWCCELKLDGLA HCCCCHHHHHHHHHHHCCCEEHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEE VSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLHGDNIPARLEVRGEVFLPQAG EEEEEECCEEEEEECCCCCCCCHHHHHCCEEEEEEEEEEECCCCCEEEEECCEEECCCCC FEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGILEGGELPDTHL HHHHHHHHHHCCCEEEECCCCCCCCCHHHCCCCCCCCCCCEEEEEEEEEECCCCCCHHHH GRLLQFKAWGLPVSDRVTLCDSPQAVLDFYHNVEKDRPTLGFDIDGVVIKVNSLALQELL HHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHH GFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRTGAITPVARLEPVQVAGVLVSNATLHN HHHHCCCCEEEEEECCCHHHHHHHHHCHHCCCCCCCCCCHHHCCCHHEEEEEEECCCCCC ADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEETRPIVFPTHCPVCGSDVERVEG HHHHHHHHHCCCCHHEEHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHHCC EAVTRCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEREYVHTPADLFRLTAG CHHHHHCCCEEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH KLTGLDRMGPKSAQNVVNALEKSKTTTFARFLYALGIREVGEATAAGLAAYFGTLEALQA HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH ATIDELQKVPDVGIVVATHVFNFFAEESNRDVIGQLLAEGVHWPAPVVINVQEIDSPFAG HHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEHHHCCCCCCC KTVVLTGSLSQMSRDDAKARLVALGAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGITVI CEEEEECCHHHHHHHHHHHHHHEECHHHHCCCCCCCCEEEECCCCCCHHHHHHHCCEEEE DEAEMIRLLGA CHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA