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The map label for this gene is ackA [H]

Identifier: 16765664

GI number: 16765664

Start: 2447939

End: 2449141

Strand: Direct

Name: ackA [H]

Synonym: STM2337

Alternate gene names: 16765664

Gene position: 2447939-2449141 (Clockwise)

Preceding gene: 16765659

Following gene: 16765665

Centisome position: 50.4

GC content: 52.29

Gene sequence:

>1203_bases
ATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCAATTATTGATGCAGTTAATGGTGA
TGAATACCTTTCTGGTTTAGCCGAATGTTTCCATCTGCCAGAAGCACGCATCAAATGGAAAATGGACGGCAGTAAACAAG
AAGCGGCTTTAGGTGCAGGCGCCGCTCACAGTGAAGCGCTGAACTTTATCGTTAACACTATTCTGGCACAAAAACCAGAA
CTGTCTGCGCAGTTAACTGCAATTGGTCACCGTATCGTACACGGCGGCGAGAAGTATACCAGCTCCGTAGTCATTGATGA
GTCCGTCATTCAGGGCATCAAAGATTCCGCCTCTTTCGCACCGCTGCATAACCCGGCTCACCTGATTGGTATCGCCGAAG
CGCTGAAATCTTTCCCGCAGCTGAAAGATAAAAACGTTGCAGTATTCGACACCGCCTTCCATCAGACCATGCCGGAAGAA
TCTTACCTGTATGCCCTGCCGTACAGCCTGTATAAAGAACACGGCGTTCGTCGCTATGGCGCACACGGCACCAGCCACTT
CTATGTAACTCAGGAAGCGGCAAAAATGCTGAACAAGCCGGTAGAAGAACTGAACATTATCACCTGCCACCTGGGCAACG
GCGGTTCTGTTTCCGCTATCCGTAACGGTAAATGCGTGGATACCTCTATGGGTCTGACCCCGCTGGAAGGCCTGGTCATG
GGTACCCGTTCCGGCGATATCGACCCTGCGATTATCTTCCACCTGCACGACACTCTGGGCATGAGCGTGGATCAGATCAA
CAAAATGCTGACCAAAGAGTCCGGTCTGCTGGGTCTGACTGAAGTGACCAGCGACTGCCGTTATGTTGAAGACAACTACG
CCACCAAAGAAGACGCTAAACGTGCGATGGACGTTTACTGCCACCGCCTGGCGAAATACATCGGTTCTTACACCGCTCTG
ATGGACGGTCGTCTGGATGCAGTCGTGTTCACCGGCGGTATCGGTGAAAACGCCGCGATGGTTCGCGAGCTGTCTCTGGG
TAAACTGGGCGTGCTGGGCTTTGAAGTCGACCACGAACGTAACCTGGCGGCCCGCTTCGGCAAATCTGGTTTCATCAACA
AAGAAGGTACCCGTCCGGCGGTGGTGATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGTCTGACTGCC
TGA

Upstream 100 bases:

>100_bases
TCCTGCGCATGATGTTAATCATAAATGTCAGGGCTATCATGCGCTACGCTCTATGGCTCACTGACGTTTTTTTAGCCACG
TATCATAAATAGGTACTTCC

Downstream 100 bases:

>100_bases
TTTCACACCGCCAGCCTCGCTGGCGGTGCTGTTTTGTAACCCGTCCCCAAAAGACGGTAACGAAAGAGGATAAACCGTGT
CCCGTATTATTATGCTGATC

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSSKLVLVLNCGSSSLKFAIIDAVNGDEYLSGLAECFHLPEARIKWKMDGSKQEAALGAGAAHSEALNFIVNTILAQKPE
LSAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDSASFAPLHNPAHLIGIAEALKSFPQLKDKNVAVFDTAFHQTMPEE
SYLYALPYSLYKEHGVRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVM
GTRSGDIDPAIIFHLHDTLGMSVDQINKMLTKESGLLGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGSYTAL
MDGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA

Sequences:

>Translated_400_residues
MSSKLVLVLNCGSSSLKFAIIDAVNGDEYLSGLAECFHLPEARIKWKMDGSKQEAALGAGAAHSEALNFIVNTILAQKPE
LSAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDSASFAPLHNPAHLIGIAEALKSFPQLKDKNVAVFDTAFHQTMPEE
SYLYALPYSLYKEHGVRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVM
GTRSGDIDPAIIFHLHDTLGMSVDQINKMLTKESGLLGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGSYTAL
MDGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA
>Mature_399_residues
SSKLVLVLNCGSSSLKFAIIDAVNGDEYLSGLAECFHLPEARIKWKMDGSKQEAALGAGAAHSEALNFIVNTILAQKPEL
SAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDSASFAPLHNPAHLIGIAEALKSFPQLKDKNVAVFDTAFHQTMPEES
YLYALPYSLYKEHGVRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVMG
TRSGDIDPAIIFHLHDTLGMSVDQINKMLTKESGLLGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGSYTALM
DGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=400, Percent_Identity=97.75, Blast_Score=812, Evalue=0.0,
Organism=Escherichia coli, GI145693184, Length=396, Percent_Identity=43.6868686868687, Blast_Score=311, Evalue=6e-86,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43258; Mature: 43127

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKLVLVLNCGSSSLKFAIIDAVNGDEYLSGLAECFHLPEARIKWKMDGSKQEAALGAG
CCCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCC
AAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDSASFA
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHCEEEECHHHHHHHHCCCCCC
PLHNPAHLIGIAEALKSFPQLKDKNVAVFDTAFHQTMPEESYLYALPYSLYKEHGVRRYG
CCCCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCCEEEECCHHHHHHCCCEECC
AHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVM
CCCCEEEEEEHHHHHHHHCCHHHCEEEEEEECCCCCEEEECCCEEEECCCCCCCCCCEEE
GTRSGDIDPAIIFHLHDTLGMSVDQINKMLTKESGLLGLTEVTSDCRYVEDNYATKEDAK
ECCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHCCCCEEHHHHHCCCCCCCCCCCCHHHHH
RAMDVYCHRLAKYIGSYTALMDGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHER
HHHHHHHHHHHHHHCCHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCEEEEEEEECCCC
NLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA
CHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEECCHHCCC
>Mature Secondary Structure 
SSKLVLVLNCGSSSLKFAIIDAVNGDEYLSGLAECFHLPEARIKWKMDGSKQEAALGAG
CCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCC
AAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDSASFA
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHCEEEECHHHHHHHHCCCCCC
PLHNPAHLIGIAEALKSFPQLKDKNVAVFDTAFHQTMPEESYLYALPYSLYKEHGVRRYG
CCCCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCCEEEECCHHHHHHCCCEECC
AHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVM
CCCCEEEEEEHHHHHHHHCCHHHCEEEEEEECCCCCEEEECCCEEEECCCCCCCCCCEEE
GTRSGDIDPAIIFHLHDTLGMSVDQINKMLTKESGLLGLTEVTSDCRYVEDNYATKEDAK
ECCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHCCCCEEHHHHHCCCCCCCCCCCCHHHHH
RAMDVYCHRLAKYIGSYTALMDGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHER
HHHHHHHHHHHHHHCCHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCEEEEEEEECCCC
NLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA
CHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]