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The map label for this gene is pmrF

Identifier: 16765625

GI number: 16765625

Start: 2405113

End: 2406096

Strand: Direct

Name: pmrF

Synonym: STM2298

Alternate gene names: 16765625

Gene position: 2405113-2406096 (Clockwise)

Preceding gene: 161353593

Following gene: 16765626

Centisome position: 49.51

GC content: 53.66

Gene sequence:

>984_bases
ATGTTTGACGCCGCCCCCATAAAAAAAGTCTCGGTGGTTATTCCTGTCTATAACGAACAGGAAAGCCTGCCGGAATTGAT
AAGACGCACCACCACCGCCTGCGAGAGCCTGGGCAAAGCGTGGGAGATTTTATTGATAGATGATGGTAGCAGCGATAGCT
CGGCGGAGCTGATGGTTAAGGCGTCCCAGGAGGCCGATAGCCATATCATCTCTATTTTACTCAACCGTAACTACGGTCAA
CATGCAGCGATTATGGCGGGGTTTAGCCATGTAAGCGGCGATCTTATCATTACGCTGGATGCCGATCTGCAAAACCCGCC
GGAAGAGATCCCGCGCCTGGTGGCGAAAGCCGATGAAGGGTTCGATGTGGTAGGCACCGTGCGCCAGAACCGACAGGACA
GCCTGTTTCGCAAATCGGCGTCAAAAATCATTAATCTGCTGATCCAGCGCACAACGGGCAAAGCGATGGGCGATTACGGC
TGCATGTTACGCGCCTATCGCCGCCCTATTATCGATACTATGTTGCGCTGCCATGAACGCAGCACCTTTATCCCTATTCT
GGCCAACATTTTCGCTCGTCGGGCTACCGAGATCCCGGTGCATCACGCTGAACGTGAATTTGGCGACTCTAAATACAGTT
TTATGCGTCTCATTAACCTGATGTATGACCTGGTGACCTGCCTGACGACGACGCCGCTGCGCCTGTTGAGTCTGCTGGGC
AGCGTTATCGCCATCGGCGGGTTTAGCCTGTCGGTATTGCTTATTGTACTTCGCCTGGCGCTGGGGCCGCAGTGGGCGGC
GGAAGGCGTATTTATGCTCTTTGCCGTTCTGTTTACCTTCATCGGCGCGCAATTCATCGGTATGGGACTGCTGGGCGAAT
ACATCGGTCGCATTTATAACGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAACAAGTTATCTACCCGGAAAGCACGCCG
TTTACTGAGGAAAGCCACCAATGA

Upstream 100 bases:

>100_bases
ACAGCGAGCGGATCTGCTCACTGCCGCTCTTCCCGGACATGACCGAGAGTGACTTTGATCGAGTCATTACCGCCCTTCAT
CAGATAGCAGGACAATAAGC

Downstream 100 bases:

>100_bases
AAGCCGTTATTTTTGCCTATCACGATATGGGATGTCAGGGGGTGCAGGCCGTACTGGATGCCGGGTATGAGATTGCCGCG
ATTTTCACTCATGCCGACAA

Product: undecaprenyl phosphate 4-deoxy-4-formamido-L-arabinose transferase

Products: NA

Alternate protein names: Undecaprenyl-phosphate Ara4FN transferase; Ara4FN transferase

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MFDAAPIKKVSVVIPVYNEQESLPELIRRTTTACESLGKAWEILLIDDGSSDSSAELMVKASQEADSHIISILLNRNYGQ
HAAIMAGFSHVSGDLIITLDADLQNPPEEIPRLVAKADEGFDVVGTVRQNRQDSLFRKSASKIINLLIQRTTGKAMGDYG
CMLRAYRRPIIDTMLRCHERSTFIPILANIFARRATEIPVHHAEREFGDSKYSFMRLINLMYDLVTCLTTTPLRLLSLLG
SVIAIGGFSLSVLLIVLRLALGPQWAAEGVFMLFAVLFTFIGAQFIGMGLLGEYIGRIYNDVRARPRYFVQQVIYPESTP
FTEESHQ

Sequences:

>Translated_327_residues
MFDAAPIKKVSVVIPVYNEQESLPELIRRTTTACESLGKAWEILLIDDGSSDSSAELMVKASQEADSHIISILLNRNYGQ
HAAIMAGFSHVSGDLIITLDADLQNPPEEIPRLVAKADEGFDVVGTVRQNRQDSLFRKSASKIINLLIQRTTGKAMGDYG
CMLRAYRRPIIDTMLRCHERSTFIPILANIFARRATEIPVHHAEREFGDSKYSFMRLINLMYDLVTCLTTTPLRLLSLLG
SVIAIGGFSLSVLLIVLRLALGPQWAAEGVFMLFAVLFTFIGAQFIGMGLLGEYIGRIYNDVRARPRYFVQQVIYPESTP
FTEESHQ
>Mature_327_residues
MFDAAPIKKVSVVIPVYNEQESLPELIRRTTTACESLGKAWEILLIDDGSSDSSAELMVKASQEADSHIISILLNRNYGQ
HAAIMAGFSHVSGDLIITLDADLQNPPEEIPRLVAKADEGFDVVGTVRQNRQDSLFRKSASKIINLLIQRTTGKAMGDYG
CMLRAYRRPIIDTMLRCHERSTFIPILANIFARRATEIPVHHAEREFGDSKYSFMRLINLMYDLVTCLTTTPLRLLSLLG
SVIAIGGFSLSVLLIVLRLALGPQWAAEGVFMLFAVLFTFIGAQFIGMGLLGEYIGRIYNDVRARPRYFVQQVIYPESTP
FTEESHQ

Specific function: Catalyzes the transfer of 4-deoxy-4-formamido-L- arabinose from UDP to undecaprenyl phosphate. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family

Homologues:

Organism=Homo sapiens, GI4503363, Length=237, Percent_Identity=28.2700421940928, Blast_Score=98, Evalue=8e-21,
Organism=Escherichia coli, GI1788588, Length=318, Percent_Identity=87.7358490566038, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI1788692, Length=312, Percent_Identity=32.6923076923077, Blast_Score=161, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI71999402, Length=225, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6325441, Length=117, Percent_Identity=29.9145299145299, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24585265, Length=235, Percent_Identity=25.531914893617, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARNC_SALA4 (B5EZH7)

Other databases:

- EMBL:   CP001138
- RefSeq:   YP_002147254.1
- ProteinModelPortal:   B5EZH7
- SMR:   B5EZH7
- PRIDE:   B5EZH7
- GeneID:   6793613
- GenomeReviews:   CP001138_GR
- KEGG:   sea:SeAg_B2434
- HOGENOM:   HBG752660
- OMA:   FSIVPLQ
- ProtClustDB:   PRK10714
- HAMAP:   MF_01164
- InterPro:   IPR022857
- InterPro:   IPR001173

Pfam domain/function: PF00535 Glycos_transf_2

EC number: =2.7.8.30

Molecular weight: Translated: 36517; Mature: 36517

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xeb75354)-; HASH(0x101df248)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDAAPIKKVSVVIPVYNEQESLPELIRRTTTACESLGKAWEILLIDDGSSDSSAELMVK
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCHHEEEE
ASQEADSHIISILLNRNYGQHAAIMAGFSHVSGDLIITLDADLQNPPEEIPRLVAKADEG
EHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCC
FDVVGTVRQNRQDSLFRKSASKIINLLIQRTTGKAMGDYGCMLRAYRRPIIDTMLRCHER
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
STFIPILANIFARRATEIPVHHAEREFGDSKYSFMRLINLMYDLVTCLTTTPLRLLSLLG
CCHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
SVIAIGGFSLSVLLIVLRLALGPQWAAEGVFMLFAVLFTFIGAQFIGMGLLGEYIGRIYN
HHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVRARPRYFVQQVIYPESTPFTEESHQ
HHHCCHHHHHHHHHCCCCCCCCCCCCH
>Mature Secondary Structure
MFDAAPIKKVSVVIPVYNEQESLPELIRRTTTACESLGKAWEILLIDDGSSDSSAELMVK
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCHHEEEE
ASQEADSHIISILLNRNYGQHAAIMAGFSHVSGDLIITLDADLQNPPEEIPRLVAKADEG
EHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCC
FDVVGTVRQNRQDSLFRKSASKIINLLIQRTTGKAMGDYGCMLRAYRRPIIDTMLRCHER
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
STFIPILANIFARRATEIPVHHAEREFGDSKYSFMRLINLMYDLVTCLTTTPLRLLSLLG
CCHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
SVIAIGGFSLSVLLIVLRLALGPQWAAEGVFMLFAVLFTFIGAQFIGMGLLGEYIGRIYN
HHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVRARPRYFVQQVIYPESTPFTEESHQ
HHHCCHHHHHHHHHCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA