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The map label for this gene is sspH2

Identifier: 16765569

GI number: 16765569

Start: 2340885

End: 2343251

Strand: Reverse

Name: sspH2

Synonym: STM2241

Alternate gene names: 16765569

Gene position: 2343251-2340885 (Counterclockwise)

Preceding gene: 16765570

Following gene: 16765568

Centisome position: 48.24

GC content: 55.34

Gene sequence:

>2367_bases
ATGCCCTTTCATATTGGAAGCGGATGTCTTCCCGCCACCATCAGTAATCGCCGCATTTATCGTATTGCCTGGTCTGATAC
CCCCCCTGAAATGAGTTCCTGGGAAAAAATGAAGGAATTTTTTTGCTCAACGCACCAGACTGAAGCGCTGGAGTGCATCT
GGACGATTTGTCACCCGCCGGCCGGAACGACGCGGGAGGATGTGATCAACAGATTTGAACTGCTCAGGACGCTCGCGTAT
GCCGGATGGGAGGAAAGCATTCATTCCGGCCAGCACGGGGAAAATTACTTCTGTATTCTGGATGAAGACAGTCAGGAGAT
ATTGTCAGTCACCCTTGATGATGCCGGGAACTATACCGTAAATTGCCAGGGGTACAGTGAAACACATCGCCTCACCCTGG
ACACAGCACAGGGTGAGGAGGGCACAGGACACGCGGAAGGGGCATCCGGGACATTCAGGACATCCTTCCTCCCTGCCACA
ACGGCTCCACAGACGCCAGCAGAGTATGATGCTGTCTGGTCAGCGTGGAGAAGGGCTGCACCCGCAGAAGAGTCACGCGG
CCGTGCAGCAGTGGTACAGAAAATGCGTGCCTGCCTGAATAATGGCAATGCAGTGCTTAACGTGGGAGAATCAGGTCTTA
CCACCTTGCCAGACTGTTTACCCGCGCATATTACCACACTGGTTATTCCTGATAATAATCTGACCAGCCTGCCGGCGCTG
CCGCCAGAACTGCGGACGCTGGAGGTCTCTGGTAACCAGCTGACTAGCCTGCCGGTGCTGCCGCCAGGACTACTGGAACT
GTCGATCTTTAGTAACCCGCTGACCCACCTGCCGGCGCTGCCGTCAGGACTATGTAAGCTGTGGATCTTTGGTAATCAAC
TGACCAGCCTGCCGGTGTTGCCGCCAGGGCTACAGGAGCTGTCGGTATCTGATAACCAACTGGCCAGCCTGCCGGCGCTG
CCGTCAGAATTATGTAAGCTGTGGGCCTATAATAACCAGCTGACCAGCCTGCCGATGTTGCCGTCAGGGCTACAGGAGCT
GTCGGTATCTGATAACCAACTGGCCAGCCTGCCGACGCTGCCGTCAGAATTATATAAGCTGTGGGCCTATAATAATCGGC
TGACCAGCCTGCCGGCGTTGCCGTCAGGACTGAAGGAGCTGATTGTATCTGGTAACCGGCTGACCAGTCTGCCGGTGCTG
CCGTCAGAACTGAAGGAGCTGATGGTATCTGGTAACCGGCTGACCAGCCTGCCGATGCTGCCGTCAGGACTACTGTCGCT
GTCGGTCTATCGTAACCAGCTGACCCGCCTGCCGGAAAGTCTCATTCATCTGTCTTCAGAGACAACCGTAAATCTGGAAG
GGAACCCACTGTCTGAACGTACTTTGCAGGCGCTGCGGGAGATCACCAGCGCGCCTGGCTATTCAGGCCCCATAATACGA
TTCGATATGGCGGGAGCCTCCGCCCCCCGGGAAACTCGGGCACTGCACCTGGCGGCCGCTGACTGGCTGGTGCCTGCCCG
GGAGGGGGAACCGGCTCCTGCAGACAGATGGCATATGTTCGGACAGGAAGATAACGCCGACGCATTCAGCCTCTTCCTGG
ACAGACTGAGTGAGACGGAAAACTTCATAAAGGACGCGGGGTTTAAGGCACAGATATCGTCCTGGCTGGCACAACTGGCT
GAAGATGAGGCGTTAAGAGCAAACACCTTTGCTATGGCAACAGAGGCAACCTCAAGCTGCGAGGACCGGGTCACATTTTT
TTTGCACCAGATGAAGAACGTACAGCTGGTACATAATGCAGAAAAAGGGCAATACGATAACGATCTCGCGGCGCTGGTTG
CCACGGGGCGTGAGATGTTCCGTCTGGGAAAACTGGAACAGATTGCCCGGGAAAAGGTCAGAACGCTGGCTCTCGTTGAT
GAAATTGAGGTCTGGCTGGCGTATCAGAATAAGCTGAAGAAATCACTCGGGCTGACCAGCGTGACGTCAGAAATGCGTTT
CTTTGACGTATCCGGCGTGACGGTTACAGACCTTCAGGACGCGGAGCTTCAGGTGAAAGCCGCTGAAAAAAGCGAGTTCA
GGGAGTGGATACTGCAGTGGGGGCCGTTACACAGAGTGCTGGAGCGCAAAGCGCCGGAACGCGTTAACGCGCTTCGTGAA
AAGCAAATATCGGATTATGAGGAAACGTACCGGATGCTGTCTGACACAGAGCTGAGACCGTCTGGGCTGGTCGGTAATAC
CGATGCAGAGCGCACTATCGGAGCAAGAGCGATGGAGAGCGCGAAAAAGACATTTTTGGATGGCCTGCGACCTCTTGTGG
AGGAGATGCTGGGGAGCTATCTGAACGTTCAGTGGCGTCGTAACTGA

Upstream 100 bases:

>100_bases
GACCATGAATCCGCCGATAACCTTATTGTGAAATTAAGACCAGGAAGAGATGATGTCTGCCGGACAGATACTATATGTAA
ATTTATAAAGGTTTTTTGTT

Downstream 100 bases:

>100_bases
TGCACCAGGTGAATGAGGTGCGGTGCGACAAAGATATTCCCGGACGAACAACATCAGACAGTACGGATGATGTACAGGTG
AAATATCCGGATGACGGCTA

Product: leucine-rich repeat-containing protein

Products: NA

Alternate protein names: Salmonella secreted protein H2; Secreted effector protein sspH2

Number of amino acids: Translated: 788; Mature: 787

Protein sequence:

>788_residues
MPFHIGSGCLPATISNRRIYRIAWSDTPPEMSSWEKMKEFFCSTHQTEALECIWTICHPPAGTTREDVINRFELLRTLAY
AGWEESIHSGQHGENYFCILDEDSQEILSVTLDDAGNYTVNCQGYSETHRLTLDTAQGEEGTGHAEGASGTFRTSFLPAT
TAPQTPAEYDAVWSAWRRAAPAEESRGRAAVVQKMRACLNNGNAVLNVGESGLTTLPDCLPAHITTLVIPDNNLTSLPAL
PPELRTLEVSGNQLTSLPVLPPGLLELSIFSNPLTHLPALPSGLCKLWIFGNQLTSLPVLPPGLQELSVSDNQLASLPAL
PSELCKLWAYNNQLTSLPMLPSGLQELSVSDNQLASLPTLPSELYKLWAYNNRLTSLPALPSGLKELIVSGNRLTSLPVL
PSELKELMVSGNRLTSLPMLPSGLLSLSVYRNQLTRLPESLIHLSSETTVNLEGNPLSERTLQALREITSAPGYSGPIIR
FDMAGASAPRETRALHLAAADWLVPAREGEPAPADRWHMFGQEDNADAFSLFLDRLSETENFIKDAGFKAQISSWLAQLA
EDEALRANTFAMATEATSSCEDRVTFFLHQMKNVQLVHNAEKGQYDNDLAALVATGREMFRLGKLEQIAREKVRTLALVD
EIEVWLAYQNKLKKSLGLTSVTSEMRFFDVSGVTVTDLQDAELQVKAAEKSEFREWILQWGPLHRVLERKAPERVNALRE
KQISDYEETYRMLSDTELRPSGLVGNTDAERTIGARAMESAKKTFLDGLRPLVEEMLGSYLNVQWRRN

Sequences:

>Translated_788_residues
MPFHIGSGCLPATISNRRIYRIAWSDTPPEMSSWEKMKEFFCSTHQTEALECIWTICHPPAGTTREDVINRFELLRTLAY
AGWEESIHSGQHGENYFCILDEDSQEILSVTLDDAGNYTVNCQGYSETHRLTLDTAQGEEGTGHAEGASGTFRTSFLPAT
TAPQTPAEYDAVWSAWRRAAPAEESRGRAAVVQKMRACLNNGNAVLNVGESGLTTLPDCLPAHITTLVIPDNNLTSLPAL
PPELRTLEVSGNQLTSLPVLPPGLLELSIFSNPLTHLPALPSGLCKLWIFGNQLTSLPVLPPGLQELSVSDNQLASLPAL
PSELCKLWAYNNQLTSLPMLPSGLQELSVSDNQLASLPTLPSELYKLWAYNNRLTSLPALPSGLKELIVSGNRLTSLPVL
PSELKELMVSGNRLTSLPMLPSGLLSLSVYRNQLTRLPESLIHLSSETTVNLEGNPLSERTLQALREITSAPGYSGPIIR
FDMAGASAPRETRALHLAAADWLVPAREGEPAPADRWHMFGQEDNADAFSLFLDRLSETENFIKDAGFKAQISSWLAQLA
EDEALRANTFAMATEATSSCEDRVTFFLHQMKNVQLVHNAEKGQYDNDLAALVATGREMFRLGKLEQIAREKVRTLALVD
EIEVWLAYQNKLKKSLGLTSVTSEMRFFDVSGVTVTDLQDAELQVKAAEKSEFREWILQWGPLHRVLERKAPERVNALRE
KQISDYEETYRMLSDTELRPSGLVGNTDAERTIGARAMESAKKTFLDGLRPLVEEMLGSYLNVQWRRN
>Mature_787_residues
PFHIGSGCLPATISNRRIYRIAWSDTPPEMSSWEKMKEFFCSTHQTEALECIWTICHPPAGTTREDVINRFELLRTLAYA
GWEESIHSGQHGENYFCILDEDSQEILSVTLDDAGNYTVNCQGYSETHRLTLDTAQGEEGTGHAEGASGTFRTSFLPATT
APQTPAEYDAVWSAWRRAAPAEESRGRAAVVQKMRACLNNGNAVLNVGESGLTTLPDCLPAHITTLVIPDNNLTSLPALP
PELRTLEVSGNQLTSLPVLPPGLLELSIFSNPLTHLPALPSGLCKLWIFGNQLTSLPVLPPGLQELSVSDNQLASLPALP
SELCKLWAYNNQLTSLPMLPSGLQELSVSDNQLASLPTLPSELYKLWAYNNRLTSLPALPSGLKELIVSGNRLTSLPVLP
SELKELMVSGNRLTSLPMLPSGLLSLSVYRNQLTRLPESLIHLSSETTVNLEGNPLSERTLQALREITSAPGYSGPIIRF
DMAGASAPRETRALHLAAADWLVPAREGEPAPADRWHMFGQEDNADAFSLFLDRLSETENFIKDAGFKAQISSWLAQLAE
DEALRANTFAMATEATSSCEDRVTFFLHQMKNVQLVHNAEKGQYDNDLAALVATGREMFRLGKLEQIAREKVRTLALVDE
IEVWLAYQNKLKKSLGLTSVTSEMRFFDVSGVTVTDLQDAELQVKAAEKSEFREWILQWGPLHRVLERKAPERVNALREK
QISDYEETYRMLSDTELRPSGLVGNTDAERTIGARAMESAKKTFLDGLRPLVEEMLGSYLNVQWRRN

Specific function: Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is an E3 ubiquitin ligase that interferes with host's ubiquitination pathway. Contributes to virulence in calves

COG id: COG4886

COG function: function code S; Leucine-rich repeat (LRR) protein

Gene ontology:

Cell location: Secreted. Host cytoplasm. Host apical cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Secreted via type III secretion system 2 (SPI-2 TTSS), and delivered into the host cytoplasm. Localizes at the periphery of the host cell, specificall

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 12 LRR (leucine-rich) repeats

Homologues:

Organism=Homo sapiens, GI110825984, Length=266, Percent_Identity=35.7142857142857, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI95113664, Length=300, Percent_Identity=31.3333333333333, Blast_Score=68, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17536887, Length=223, Percent_Identity=41.2556053811659, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25143890, Length=269, Percent_Identity=29.7397769516729, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSPH2_SALT1 (D0ZPH9)

Other databases:

- EMBL:   AF160727
- EMBL:   CP001363
- ProteinModelPortal:   D0ZPH9
- PRIDE:   D0ZPH9
- GenomeReviews:   CP001363_GR
- GO:   GO:0016020
- GO:   GO:0009405
- InterPro:   IPR001611

Pfam domain/function: PF00560 LRR_1

EC number: NA

Molecular weight: Translated: 87224; Mature: 87093

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS51450 LRR

Important sites: ACT_SITE 580-580

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFHIGSGCLPATISNRRIYRIAWSDTPPEMSSWEKMKEFFCSTHQTEALECIWTICHPP
CCCCCCCCCCCCEECCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
AGTTREDVINRFELLRTLAYAGWEESIHSGQHGENYFCILDEDSQEILSVTLDDAGNYTV
CCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCEEEEECCCCCEEEEEEECCCCCEEE
NCQGYSETHRLTLDTAQGEEGTGHAEGASGTFRTSFLPATTAPQTPAEYDAVWSAWRRAA
EECCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCC
PAEESRGRAAVVQKMRACLNNGNAVLNVGESGLTTLPDCLPAHITTLVIPDNNLTSLPAL
CCHHHCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHCCEEEEEECCCCCCCCCCC
PPELRTLEVSGNQLTSLPVLPPGLLELSIFSNPLTHLPALPSGLCKLWIFGNQLTSLPVL
CCCCEEEEECCCCEEECCCCCCCCEEEEHHCCCHHHCCCCCHHHEEEEEECCCCCCCCCC
PPGLQELSVSDNQLASLPALPSELCKLWAYNNQLTSLPMLPSGLQELSVSDNQLASLPTL
CCCHHHCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCHHHCCCCC
PSELYKLWAYNNRLTSLPALPSGLKELIVSGNRLTSLPVLPSELKELMVSGNRLTSLPML
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCEEECCCC
PSGLLSLSVYRNQLTRLPESLIHLSSETTVNLEGNPLSERTLQALREITSAPGYSGPIIR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEE
FDMAGASAPRETRALHLAAADWLVPAREGEPAPADRWHMFGQEDNADAFSLFLDRLSETE
EECCCCCCCCHHHEEEEEHHHHEEECCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHH
NFIKDAGFKAQISSWLAQLAEDEALRANTFAMATEATSSCEDRVTFFLHQMKNVQLVHNA
HHHHHCCCHHHHHHHHHHHHHCHHHHHHHHEEEHHHHHHHHHHHHHHHHHHCCEEEEECC
EKGQYDNDLAALVATGREMFRLGKLEQIAREKVRTLALVDEIEVWLAYQNKLKKSLGLTS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VTSEMRFFDVSGVTVTDLQDAELQVKAAEKSEFREWILQWGPLHRVLERKAPERVNALRE
HHHCCEEEECCCCEEECCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHH
KQISDYEETYRMLSDTELRPSGLVGNTDAERTIGARAMESAKKTFLDGLRPLVEEMLGSY
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNVQWRRN
CCEEEECC
>Mature Secondary Structure 
PFHIGSGCLPATISNRRIYRIAWSDTPPEMSSWEKMKEFFCSTHQTEALECIWTICHPP
CCCCCCCCCCCEECCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
AGTTREDVINRFELLRTLAYAGWEESIHSGQHGENYFCILDEDSQEILSVTLDDAGNYTV
CCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCEEEEECCCCCEEEEEEECCCCCEEE
NCQGYSETHRLTLDTAQGEEGTGHAEGASGTFRTSFLPATTAPQTPAEYDAVWSAWRRAA
EECCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCC
PAEESRGRAAVVQKMRACLNNGNAVLNVGESGLTTLPDCLPAHITTLVIPDNNLTSLPAL
CCHHHCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHCCEEEEEECCCCCCCCCCC
PPELRTLEVSGNQLTSLPVLPPGLLELSIFSNPLTHLPALPSGLCKLWIFGNQLTSLPVL
CCCCEEEEECCCCEEECCCCCCCCEEEEHHCCCHHHCCCCCHHHEEEEEECCCCCCCCCC
PPGLQELSVSDNQLASLPALPSELCKLWAYNNQLTSLPMLPSGLQELSVSDNQLASLPTL
CCCHHHCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCHHHCCCCC
PSELYKLWAYNNRLTSLPALPSGLKELIVSGNRLTSLPVLPSELKELMVSGNRLTSLPML
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCEEECCCC
PSGLLSLSVYRNQLTRLPESLIHLSSETTVNLEGNPLSERTLQALREITSAPGYSGPIIR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEE
FDMAGASAPRETRALHLAAADWLVPAREGEPAPADRWHMFGQEDNADAFSLFLDRLSETE
EECCCCCCCCHHHEEEEEHHHHEEECCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHH
NFIKDAGFKAQISSWLAQLAEDEALRANTFAMATEATSSCEDRVTFFLHQMKNVQLVHNA
HHHHHCCCHHHHHHHHHHHHHCHHHHHHHHEEEHHHHHHHHHHHHHHHHHHCCEEEEECC
EKGQYDNDLAALVATGREMFRLGKLEQIAREKVRTLALVDEIEVWLAYQNKLKKSLGLTS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VTSEMRFFDVSGVTVTDLQDAELQVKAAEKSEFREWILQWGPLHRVLERKAPERVNALRE
HHHCCEEEECCCCEEECCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHH
KQISDYEETYRMLSDTELRPSGLVGNTDAERTIGARAMESAKKTFLDGLRPLVEEMLGSY
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNVQWRRN
CCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10564523; 10861017