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The map label for this gene is pifA [C]

Identifier: 16765566

GI number: 16765566

Start: 2337824

End: 2339155

Strand: Direct

Name: pifA [C]

Synonym: STM2238

Alternate gene names: 16765566

Gene position: 2337824-2339155 (Clockwise)

Preceding gene: 16765560

Following gene: 16765572

Centisome position: 48.13

GC content: 33.48

Gene sequence:

>1332_bases
ATGTTAGAAACACTATCATTCACTGAACGTGACGAGTTCCAACGAAGAAACATCGCTGAAAATATCATCAAATTGCTAAA
ACCAGAGGCAGACATTTCACCACTGGTAATAGACGGTGCATGGGGGACAGGGAAATCAGAATTTAGTATCAAACTGAAGA
ATCTCATTATCGAGCAAGAAACTGAGTCTAAAGTTGTCTATGTTGATGCCTTTAAAGGGGATCATGCAGAATCTCCATTA
CTTCTAATAACATCAGCTATTGCGAGCATTTTGCCGGAGGAGGAAAAACAAAACTTCATTAAGAGATCTCTTCCTGCAAT
TCGATTTGGTTTAAAAACTGTACTAAAAGCTGGTGCAGGTTGGTTTTTACGACAGGAGGCTAGTGAAGTTGCCGAAGAAT
TCCAAGATGCGATGAAGAAAGCAAGCAATGCAGCAATAGATGGGACTATTGAAAATATACTTGAAGACCACATGGAATCA
GAGAAAAACATAAACTCTCTAAAATCTTGCATTGAAGATATATCAAACAAGCAAAAGATAGTGATAATTATTGATGAATT
AGACCGATGTAAGCCAAGTTTTTCAACAAATATCATTGAAACAATAAAACACATCTTTGATATCAATAACGTATTTTTTA
TTTTAGTTACAAACACTGAACAACTAAAAGCATCTATAAATCATATTTATGGTTATAGCATTAACTCACAAAAGTATCTT
GACAAGTTTATAAAATATACCATTACTCTTCCAGACACATGCTTAATAAATGGGCATAACGTATGTAAAACCTCGGTTAT
ATATTGGGATCACCTTGTAGGTGAAACTACATTACTAAATAAAATCAACAGCTTAGTTGGTAGCTTTATTTGTGATTTAA
TTCAACGAACCAACTTATCCTTACGTGAAACACAAACCTTTTCACGCAATCTTAACATTTTCCGACTGTTAAACGACAAT
GAGTGCAAAAGCAATGATCCTTTTATAAATATGATCGTTGTCGTAGCTGTCTTCATACATTGTTTTGGTGACAAGGAAAA
ACTAAAACAAGAAATTACCGCTGAATCTATATCTTATTTAGCAGACCTGCTTAACATAAAAGAAATACCTTATTCTTATG
AGAGAAGATCGCAAATCCCTGAAATATCAATTATATTCTTCGGAATAATTAAAGACAGCATTACTCTTAATGAGCGATTT
GCCCCTAAAAGTGATGAGGAACTTAAAAAGTTCACAAATGTTTATACTGATTATGAACACCTAAAATTTTGGAGCACTAC
TCCCAGAGAGTTAATGATAAAATATATTAATCAAATGTCATTCATCCAGTAA

Upstream 100 bases:

>100_bases
TCTTAGCAGATTACAAGAATTTTTGCGGACCGCGTTAATGATTTTTAATCCCAAAGTCGTATTATGCGTTTTTTACTGTA
GGAGTTAACAGGCTTATATT

Downstream 100 bases:

>100_bases
ATAATACGCCCCTGCATGGGGCGTATTATTAAACGTCCATATCTAATGTTAGATCCAGCATTGAAAGGCAGCCATCAATA
AATCCTTCGGCTAACTGTAT

Product: hypothetical protein

Products: NA

Alternate protein names: P-Loop ATPase; KAP Family P-Loop Domain Protein; KAP P-Loop Domain Protein; KAP P-Loop; KAP Family P-Loop Domain-Containing Protein; Kap P-Loop Domain Protein; Phage T7 Exclusion Protein

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MLETLSFTERDEFQRRNIAENIIKLLKPEADISPLVIDGAWGTGKSEFSIKLKNLIIEQETESKVVYVDAFKGDHAESPL
LLITSAIASILPEEEKQNFIKRSLPAIRFGLKTVLKAGAGWFLRQEASEVAEEFQDAMKKASNAAIDGTIENILEDHMES
EKNINSLKSCIEDISNKQKIVIIIDELDRCKPSFSTNIIETIKHIFDINNVFFILVTNTEQLKASINHIYGYSINSQKYL
DKFIKYTITLPDTCLINGHNVCKTSVIYWDHLVGETTLLNKINSLVGSFICDLIQRTNLSLRETQTFSRNLNIFRLLNDN
ECKSNDPFINMIVVVAVFIHCFGDKEKLKQEITAESISYLADLLNIKEIPYSYERRSQIPEISIIFFGIIKDSITLNERF
APKSDEELKKFTNVYTDYEHLKFWSTTPRELMIKYINQMSFIQ

Sequences:

>Translated_443_residues
MLETLSFTERDEFQRRNIAENIIKLLKPEADISPLVIDGAWGTGKSEFSIKLKNLIIEQETESKVVYVDAFKGDHAESPL
LLITSAIASILPEEEKQNFIKRSLPAIRFGLKTVLKAGAGWFLRQEASEVAEEFQDAMKKASNAAIDGTIENILEDHMES
EKNINSLKSCIEDISNKQKIVIIIDELDRCKPSFSTNIIETIKHIFDINNVFFILVTNTEQLKASINHIYGYSINSQKYL
DKFIKYTITLPDTCLINGHNVCKTSVIYWDHLVGETTLLNKINSLVGSFICDLIQRTNLSLRETQTFSRNLNIFRLLNDN
ECKSNDPFINMIVVVAVFIHCFGDKEKLKQEITAESISYLADLLNIKEIPYSYERRSQIPEISIIFFGIIKDSITLNERF
APKSDEELKKFTNVYTDYEHLKFWSTTPRELMIKYINQMSFIQ
>Mature_443_residues
MLETLSFTERDEFQRRNIAENIIKLLKPEADISPLVIDGAWGTGKSEFSIKLKNLIIEQETESKVVYVDAFKGDHAESPL
LLITSAIASILPEEEKQNFIKRSLPAIRFGLKTVLKAGAGWFLRQEASEVAEEFQDAMKKASNAAIDGTIENILEDHMES
EKNINSLKSCIEDISNKQKIVIIIDELDRCKPSFSTNIIETIKHIFDINNVFFILVTNTEQLKASINHIYGYSINSQKYL
DKFIKYTITLPDTCLINGHNVCKTSVIYWDHLVGETTLLNKINSLVGSFICDLIQRTNLSLRETQTFSRNLNIFRLLNDN
ECKSNDPFINMIVVVAVFIHCFGDKEKLKQEITAESISYLADLLNIKEIPYSYERRSQIPEISIIFFGIIKDSITLNERF
APKSDEELKKFTNVYTDYEHLKFWSTTPRELMIKYINQMSFIQ

Specific function: Responsible For The Exclusion Of Phage T7 By Plasmid F. Growth Of Bacteriophage T7 Is Inhibited In Cells Of E. coli That Carries The Plasmid F. [C]

COG id: COG4928

COG function: function code R; Predicted P-loop ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 50871; Mature: 50871

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLETLSFTERDEFQRRNIAENIIKLLKPEADISPLVIDGAWGTGKSEFSIKLKNLIIEQE
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHEEEHHHHHEECC
TESKVVYVDAFKGDHAESPLLLITSAIASILPEEEKQNFIKRSLPAIRFGLKTVLKAGAG
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCC
WFLRQEASEVAEEFQDAMKKASNAAIDGTIENILEDHMESEKNINSLKSCIEDISNKQKI
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEE
VIIIDELDRCKPSFSTNIIETIKHIFDINNVFFILVTNTEQLKASINHIYGYSINSQKYL
EEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHCCCCCCCHHHH
DKFIKYTITLPDTCLINGHNVCKTSVIYWDHLVGETTLLNKINSLVGSFICDLIQRTNLS
HHHHHEEEECCCEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCC
LRETQTFSRNLNIFRLLNDNECKSNDPFINMIVVVAVFIHCFGDKEKLKQEITAESISYL
HHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ADLLNIKEIPYSYERRSQIPEISIIFFGIIKDSITLNERFAPKSDEELKKFTNVYTDYEH
HHHHHHHHCCCCHHHHCCCCCEEEEEEEHHHCCEEECCCCCCCCHHHHHHHHHHHCCHHH
LKFWSTTPRELMIKYINQMSFIQ
HEECCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLETLSFTERDEFQRRNIAENIIKLLKPEADISPLVIDGAWGTGKSEFSIKLKNLIIEQE
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHEEEHHHHHEECC
TESKVVYVDAFKGDHAESPLLLITSAIASILPEEEKQNFIKRSLPAIRFGLKTVLKAGAG
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCC
WFLRQEASEVAEEFQDAMKKASNAAIDGTIENILEDHMESEKNINSLKSCIEDISNKQKI
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEE
VIIIDELDRCKPSFSTNIIETIKHIFDINNVFFILVTNTEQLKASINHIYGYSINSQKYL
EEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHCCCCCCCHHHH
DKFIKYTITLPDTCLINGHNVCKTSVIYWDHLVGETTLLNKINSLVGSFICDLIQRTNLS
HHHHHEEEECCCEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCC
LRETQTFSRNLNIFRLLNDNECKSNDPFINMIVVVAVFIHCFGDKEKLKQEITAESISYL
HHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ADLLNIKEIPYSYERRSQIPEISIIFFGIIKDSITLNERFAPKSDEELKKFTNVYTDYEH
HHHHHHHHCCCCHHHHCCCCCEEEEEEEHHHCCEEECCCCCCCCHHHHHHHHHHHCCHHH
LKFWSTTPRELMIKYINQMSFIQ
HEECCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA