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The map label for this gene is galS

Identifier: 16765521

GI number: 16765521

Start: 2288115

End: 2289137

Strand: Reverse

Name: galS

Synonym: STM2191

Alternate gene names: 16765521

Gene position: 2289137-2288115 (Counterclockwise)

Preceding gene: 16765522

Following gene: 16765520

Centisome position: 47.13

GC content: 55.72

Gene sequence:

>1023_bases
ATGATCACCATTCGTGATGTAGCGCGCCAGGCTGGCGTGTCTGTAGCGACCGTTTCCCGCGTACTGAATAACAGCGCGTT
GGTCAGTCCCGACACCCGTGACGCCGTTATGCAGGCCGTCACCCTGCTGGGATATCGGCCAAATGCGAATGCGCAAGCGC
TGGCCACTCAGGTGAGCGACACCATCGGCGTCGTGGTTATGGATGTTTCCGATGCCTTTTTCGGCGCTCTGGTGAAAGCC
GTAGATCTGGTCGCGCAGCAGCACCAGAAATATGTTCTCATTGGCAACAGTTATCATGAGGCGGAAAAAGAGCGCCATGC
GATTGAAGTCTTGATCCGTCAGCGTTGTAACGCATTGATTGTTCACTCAAAAGCCTTAACCGATCGCGAGCTGAGCGACT
TTATGGATCAGATCCCCGGTATGGTGCTGATTAACCGTATCGTGCCGGGTTATGCGCATCGTTGTGTTTGTCTCGACAAT
GTGAGCGGCGCCAGAATGGCGACCCGAATGTTGCTGAATAATGGACATCAACGCATCGGCTACCTGGCCTCCAGCCACCG
TATTGAAGATGACGCGATGCGCAGAGAAGGGTGGTTACACGCGCTGCAAGAGCAGGGGATTGCTGCGTCGGAGAGCTGGA
TAGGCACCGGCACGCCGGACATGCAGGGCGGCGAGTCGGCAATGGTTGAGTTGCTGGGACGCAATCTGCAACTGACGGCG
GTATTTGCCTATAACGATAACATGGCGGCGGGCGCGCTGACGGCGTTAAAAGATAACGGCATCGCCATTCCCTTGCATCT
GTCTGTCATCGGTTTCGATGATATCCCTATTGCTCGTTATACCGATCCTCAGTTGACTACCGTGCGCTATCCTATTGCTT
CTATGGCGAAAATCGCGACCGAGCTGGCGTTACAGGGGGCCGCAGGCACGCTGGATATCACGGCGACGCACTGCTTTATG
CCGACCCTGGTACGGCGCCATTCGGTGGCATGGCGACAGAATGCGGTACTGATCACTAACTGA

Upstream 100 bases:

>100_bases
TGTGATGTTTCTCACGCAGCCCTGGGCAGGGTGCTGACAAAATAGCCGCCTGCTAACGGGGCATTTGTGAGCATTTTTGA
ACTCAAACAGGGCAGTGAAT

Downstream 100 bases:

>100_bases
TTACGCACCGCATGTAACCGTTTTCAATCTGTGAGTAAATTCACAGTTTATTAACATTGTGATAGCTATGATGACAACGT
TTGTCGCACTGTAACTAACG

Product: DNA-binding transcriptional regulator GalS

Products: NA

Alternate protein names: Mgl repressor and galactose ultrainduction factor

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MITIRDVARQAGVSVATVSRVLNNSALVSPDTRDAVMQAVTLLGYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKA
VDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALTDRELSDFMDQIPGMVLINRIVPGYAHRCVCLDN
VSGARMATRMLLNNGHQRIGYLASSHRIEDDAMRREGWLHALQEQGIAASESWIGTGTPDMQGGESAMVELLGRNLQLTA
VFAYNDNMAAGALTALKDNGIAIPLHLSVIGFDDIPIARYTDPQLTTVRYPIASMAKIATELALQGAAGTLDITATHCFM
PTLVRRHSVAWRQNAVLITN

Sequences:

>Translated_340_residues
MITIRDVARQAGVSVATVSRVLNNSALVSPDTRDAVMQAVTLLGYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKA
VDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALTDRELSDFMDQIPGMVLINRIVPGYAHRCVCLDN
VSGARMATRMLLNNGHQRIGYLASSHRIEDDAMRREGWLHALQEQGIAASESWIGTGTPDMQGGESAMVELLGRNLQLTA
VFAYNDNMAAGALTALKDNGIAIPLHLSVIGFDDIPIARYTDPQLTTVRYPIASMAKIATELALQGAAGTLDITATHCFM
PTLVRRHSVAWRQNAVLITN
>Mature_340_residues
MITIRDVARQAGVSVATVSRVLNNSALVSPDTRDAVMQAVTLLGYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKA
VDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALTDRELSDFMDQIPGMVLINRIVPGYAHRCVCLDN
VSGARMATRMLLNNGHQRIGYLASSHRIEDDAMRREGWLHALQEQGIAASESWIGTGTPDMQGGESAMVELLGRNLQLTA
VFAYNDNMAAGALTALKDNGIAIPLHLSVIGFDDIPIARYTDPQLTTVRYPIASMAKIATELALQGAAGTLDITATHCFM
PTLVRRHSVAWRQNAVLITN

Specific function: Repressor of the mgl operon. Binds galactose and D- fucose as inducers. GalS binds to an operator DNA sequence within its own coding sequence

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1788474, Length=340, Percent_Identity=88.8235294117647, Blast_Score=625, Evalue=1e-180,
Organism=Escherichia coli, GI1789202, Length=330, Percent_Identity=56.969696969697, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1789068, Length=334, Percent_Identity=36.8263473053892, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1790369, Length=307, Percent_Identity=33.5504885993485, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=31.8318318318318, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1790194, Length=304, Percent_Identity=29.2763157894737, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1787580, Length=327, Percent_Identity=29.0519877675841, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI48994940, Length=311, Percent_Identity=26.0450160771704, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1786540, Length=346, Percent_Identity=29.4797687861272, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787906, Length=261, Percent_Identity=29.1187739463602, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=24.1157556270096, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789456, Length=306, Percent_Identity=26.797385620915, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1790689, Length=291, Percent_Identity=25.7731958762887, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GALS_SALTY (P41030)

Other databases:

- EMBL:   AE006468
- RefSeq:   NP_461136.1
- ProteinModelPortal:   P41030
- SMR:   P41030
- PRIDE:   P41030
- GeneID:   1253713
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2191
- HOGENOM:   HBG753640
- OMA:   QGGEAAM
- ProtClustDB:   PRK10401
- BioCyc:   STYP99287:STM2191-MONOMER
- GO:   GO:0005622
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- PRINTS:   PR00036
- SMART:   SM00354

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 36964; Mature: 36964

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2; PS00134 TRYPSIN_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIRDVARQAGVSVATVSRVLNNSALVSPDTRDAVMQAVTLLGYRPNANAQALATQVSD
CEEHHHHHHHHCCHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
TIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALI
CEEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEE
VHSKALTDRELSDFMDQIPGMVLINRIVPGYAHRCVCLDNVSGARMATRMLLNNGHQRIG
EECHHCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHH
YLASSHRIEDDAMRREGWLHALQEQGIAASESWIGTGTPDMQGGESAMVELLGRNLQLTA
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEE
VFAYNDNMAAGALTALKDNGIAIPLHLSVIGFDDIPIARYTDPQLTTVRYPIASMAKIAT
EEEECCCCCHHHHEEECCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHH
ELALQGAAGTLDITATHCFMPTLVRRHSVAWRQNAVLITN
HHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCEEEEEC
>Mature Secondary Structure
MITIRDVARQAGVSVATVSRVLNNSALVSPDTRDAVMQAVTLLGYRPNANAQALATQVSD
CEEHHHHHHHHCCHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
TIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALI
CEEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEE
VHSKALTDRELSDFMDQIPGMVLINRIVPGYAHRCVCLDNVSGARMATRMLLNNGHQRIG
EECHHCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHH
YLASSHRIEDDAMRREGWLHALQEQGIAASESWIGTGTPDMQGGESAMVELLGRNLQLTA
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEE
VFAYNDNMAAGALTALKDNGIAIPLHLSVIGFDDIPIARYTDPQLTTVRYPIASMAKIAT
EEEECCCCCHHHHEEECCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHH
ELALQGAAGTLDITATHCFMPTLVRRHSVAWRQNAVLITN
HHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3146019