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The map label for this gene is yohI [H]

Identifier: 16765504

GI number: 16765504

Start: 2270724

End: 2271662

Strand: Reverse

Name: yohI [H]

Synonym: STM2174

Alternate gene names: 16765504

Gene position: 2271662-2270724 (Counterclockwise)

Preceding gene: 16765505

Following gene: 16765502

Centisome position: 46.77

GC content: 57.29

Gene sequence:

>939_bases
ATGCGTGTTTTACTGGCGCCGATGGAAGGCGTGCTCGACGCGTTAGTGCGCGAGCTGCTGACCGAAGTGAATGATTACGA
TCTCTGCATCACCGAATTTGTGCGCGTGGTGGATCAGCTGCTGCCGGTAAAAGTGTTTCATCGCATCTGCCCGGAGTTGC
ATCACGCCAGCCGCACGCCGTCCGGCACGCCGGTGCGTATTCAGCTTCTGGGCCAGCATCCGCAGTGGCTGGCGGAAAAC
GCCGCGCGGGCGACGGCGTTGGGATCGTATGGCGTGGACCTGAACTGCGGCTGTCCGTCAAAAGTGGTGAACGGCAGCGG
CGGCGGCGCGACATTGCTCAAAGATCCCGAACTCATCTATCAGGGCGCGAAAGCGATGCGGGCCGCGGTACCGTCGCATC
TTCCGGTGACGGTAAAAGTGCGTCTCGGCTGGGATAGCGGCGATAGAAAATTTGAAATCGCCGATGCGGTGCAGCAGGCT
GGCGCCAGTGAACTGGTGGTGCATGGTCGTACCAAAGCGCAGGGCTACCGCGCCGAGCATATCGACTGGCAGGCGATCGG
CGAAATACGCCAGCGTCTGACTATTCCGGTTATCGCGAATGGCGAAATCTTGGACTGGCAGAGCGCGCAGGCATGTATGG
CGACCAGCGGCTGCGATGCGGTGATGATTGGCCGTGGGGCGTTAAATATTCCTAACCTGAGCCGGGTGGTGAAGTATAAC
GAGCCGCGTATGCCGTGGCCGGAAGTGGTGACGTTATTACAAAAATATACCCGACTGGAAAAGCAGGGCGATACCGGTTT
ATATCATGTCGCGCGTATTAAACAGTGGCTGGGATATTTACGTAAGGAATATATTGAGGCGACGGAACTCTTTCAGTCGA
TTCGGGCGTTAAACCGTTCGTCCGAGATTGCGCGGGCGATTCAGGCTATTAAAATCTAA

Upstream 100 bases:

>100_bases
CGTGACGTAGCGTGGCGTTTGCGCCGCCACCCGGCAAAAAGTTTTGCTCATCGCAGAGAGGCGTTATGATAGCGCCTCTT
TTTTTGCTGTGGATACCGAT

Downstream 100 bases:

>100_bases
TCTCTACAGCGTCCAGAACGTGTGCCGGGCAGATACTATCGCTGCCCGGCGCAGGAATCAGAAAATCATCTTAAACACGC
CCGTTACCACCAACAGCCCA

Product: tRNA-dihydrouridine synthase C

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MRVLLAPMEGVLDALVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELHHASRTPSGTPVRIQLLGQHPQWLAEN
AARATALGSYGVDLNCGCPSKVVNGSGGGATLLKDPELIYQGAKAMRAAVPSHLPVTVKVRLGWDSGDRKFEIADAVQQA
GASELVVHGRTKAQGYRAEHIDWQAIGEIRQRLTIPVIANGEILDWQSAQACMATSGCDAVMIGRGALNIPNLSRVVKYN
EPRMPWPEVVTLLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYIEATELFQSIRALNRSSEIARAIQAIKI

Sequences:

>Translated_312_residues
MRVLLAPMEGVLDALVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELHHASRTPSGTPVRIQLLGQHPQWLAEN
AARATALGSYGVDLNCGCPSKVVNGSGGGATLLKDPELIYQGAKAMRAAVPSHLPVTVKVRLGWDSGDRKFEIADAVQQA
GASELVVHGRTKAQGYRAEHIDWQAIGEIRQRLTIPVIANGEILDWQSAQACMATSGCDAVMIGRGALNIPNLSRVVKYN
EPRMPWPEVVTLLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYIEATELFQSIRALNRSSEIARAIQAIKI
>Mature_312_residues
MRVLLAPMEGVLDALVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELHHASRTPSGTPVRIQLLGQHPQWLAEN
AARATALGSYGVDLNCGCPSKVVNGSGGGATLLKDPELIYQGAKAMRAAVPSHLPVTVKVRLGWDSGDRKFEIADAVQQA
GASELVVHGRTKAQGYRAEHIDWQAIGEIRQRLTIPVIANGEILDWQSAQACMATSGCDAVMIGRGALNIPNLSRVVKYN
EPRMPWPEVVTLLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYIEATELFQSIRALNRSSEIARAIQAIKI

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=231, Percent_Identity=29.004329004329, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI40807366, Length=239, Percent_Identity=28.8702928870293, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI8923374, Length=287, Percent_Identity=27.1777003484321, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI239788483, Length=177, Percent_Identity=31.638418079096, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI239788462, Length=177, Percent_Identity=31.638418079096, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1788462, Length=312, Percent_Identity=89.7435897435898, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI1789660, Length=322, Percent_Identity=29.5031055900621, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI145693211, Length=249, Percent_Identity=27.710843373494, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25144369, Length=215, Percent_Identity=32.5581395348837, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17543114, Length=240, Percent_Identity=26.25, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17507177, Length=144, Percent_Identity=34.0277777777778, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=202, Percent_Identity=29.2079207920792, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI19921524, Length=145, Percent_Identity=37.2413793103448, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24585320, Length=209, Percent_Identity=32.5358851674641, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24580595, Length=179, Percent_Identity=30.7262569832402, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19920448, Length=179, Percent_Identity=30.7262569832402, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34518; Mature: 34518

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLLAPMEGVLDALVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELHHASRTP
CEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SGTPVRIQLLGQHPQWLAENAARATALGSYGVDLNCGCPSKVVNGSGGGATLLKDPELIY
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEECCCCCHHHCCCCCCCCEEECCHHHHH
QGAKAMRAAVPSHLPVTVKVRLGWDSGDRKFEIADAVQQAGASELVVHGRTKAQGYRAEH
HHHHHHHHHCCCCCCEEEEEEECCCCCCCEEHHHHHHHHCCCCCEEEECCCCCCCCCCCC
IDWQAIGEIRQRLTIPVIANGEILDWQSAQACMATSGCDAVMIGRGALNIPNLSRVVKYN
CCHHHHHHHHHHEECEEEECCCEECCCCHHHHHHCCCCCEEEECCCCCCCCCHHHHHCCC
EPRMPWPEVVTLLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYIEATELFQSIRALNRS
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
SEIARAIQAIKI
HHHHHHHHHHCC
>Mature Secondary Structure
MRVLLAPMEGVLDALVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELHHASRTP
CEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SGTPVRIQLLGQHPQWLAENAARATALGSYGVDLNCGCPSKVVNGSGGGATLLKDPELIY
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEECCCCCHHHCCCCCCCCEEECCHHHHH
QGAKAMRAAVPSHLPVTVKVRLGWDSGDRKFEIADAVQQAGASELVVHGRTKAQGYRAEH
HHHHHHHHHCCCCCCEEEEEEECCCCCCCEEHHHHHHHHCCCCCEEEECCCCCCCCCCCC
IDWQAIGEIRQRLTIPVIANGEILDWQSAQACMATSGCDAVMIGRGALNIPNLSRVVKYN
CCHHHHHHHHHHEECEEEECCCEECCCCHHHHHHCCCCCEEEECCCCCCCCCHHHHHCCC
EPRMPWPEVVTLLQKYTRLEKQGDTGLYHVARIKQWLGYLRKEYIEATELFQSIRALNRS
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
SEIARAIQAIKI
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]