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The map label for this gene is wcaH [H]

Identifier: 16765437

GI number: 16765437

Start: 2191160

End: 2191663

Strand: Reverse

Name: wcaH [H]

Synonym: STM2107

Alternate gene names: 16765437

Gene position: 2191663-2191160 (Counterclockwise)

Preceding gene: 16765438

Following gene: 16765436

Centisome position: 45.12

GC content: 59.92

Gene sequence:

>504_bases
TTGAGAATCAGCAACGGTTCCGGGGGTGACAATGTTTTTACGTCAGGAAGATTTCGCCGCCGTGGTGCGGCCACGCCCCT
CATCTCCCTCGATTTCATCGTGGAAAACGGCCAGGGGGAAATTTTACTGGGCCAGCGTCTCAACCGTCCGGCGCAGGGCT
ACTGGTTTGTGCCGGGGGGGCGGGTGTGCAAAGACGAAACGCTGGAGGCCGCCTTTGCACGCCTGACGCAGGCGGAACTG
GGCGTGCGTCTGCCGCTGGCGGCAGGGACGTTTTATGGCGTCTGGCAGCACTTCTATGACGACAACTTTTCCGGTGAGGA
TTTTTCAACTCACTACATCGTGCTCGGCTTTCGTCTGCGCGTGGCGGAGAGCGATTTACGCCTGCCTGATGCCCAGCATG
GCAGTTACCGCTGGCTGACGCCGGAACAGCTTCTGGCAAGCGACAATGTTCATGAGAACAGTCGGGCCTACTTCTCTCCT
GACGCGCCGGCGGTGGGCCTATGA

Upstream 100 bases:

>100_bases
ACGCCGCGTAAATTGCTCGACGTCACGCGCCTGCATCAGCTTGGCTGGTATCACGAAATTTCACTGGAGGCAGGGCTTGC
CGGTACTTACCAGTGGTTCC

Downstream 100 bases:

>100_bases
AGATCCTGGTCTATGGCATTAACTATTCGCCGGAACTGACCGGTATCGGCAAATATACCGGCGAAATGGTGGCGTGGATG
GCGCAGGAAGGCCATGAGGT

Product: GDP-mannose mannosyl hydrolase

Products: GDP; mannose [C]

Alternate protein names: GDPMH; Colanic acid biosynthesis protein wcaH [H]

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MRISNGSGGDNVFTSGRFRRRGAATPLISLDFIVENGQGEILLGQRLNRPAQGYWFVPGGRVCKDETLEAAFARLTQAEL
GVRLPLAAGTFYGVWQHFYDDNFSGEDFSTHYIVLGFRLRVAESDLRLPDAQHGSYRWLTPEQLLASDNVHENSRAYFSP
DAPAVGL

Sequences:

>Translated_167_residues
MRISNGSGGDNVFTSGRFRRRGAATPLISLDFIVENGQGEILLGQRLNRPAQGYWFVPGGRVCKDETLEAAFARLTQAEL
GVRLPLAAGTFYGVWQHFYDDNFSGEDFSTHYIVLGFRLRVAESDLRLPDAQHGSYRWLTPEQLLASDNVHENSRAYFSP
DAPAVGL
>Mature_167_residues
MRISNGSGGDNVFTSGRFRRRGAATPLISLDFIVENGQGEILLGQRLNRPAQGYWFVPGGRVCKDETLEAAFARLTQAEL
GVRLPLAAGTFYGVWQHFYDDNFSGEDFSTHYIVLGFRLRVAESDLRLPDAQHGSYRWLTPEQLLASDNVHENSRAYFSP
DAPAVGL

Specific function: It could participate in the regulation of cell wall biosynthesis by influencing the concentration of GDP-mannose or GDP-glucose in the cell. May be involved in the degradation of GDP-mannose and GDP-glucose, diverting the GDP to the synthesis of GDP-fucos

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI87082031, Length=135, Percent_Identity=78.5185185185185, Blast_Score=229, Evalue=7e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021161
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: NA

Molecular weight: Translated: 18567; Mature: 18567

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRISNGSGGDNVFTSGRFRRRGAATPLISLDFIVENGQGEILLGQRLNRPAQGYWFVPGG
CCCCCCCCCCCCEECCCCCCCCCCCCEEEEEEEEECCCCCEEECCCCCCCCCCEEECCCC
RVCKDETLEAAFARLTQAELGVRLPLAAGTFYGVWQHFYDDNFSGEDFSTHYIVLGFRLR
CCCCHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEE
VAESDLRLPDAQHGSYRWLTPEQLLASDNVHENSRAYFSPDAPAVGL
EECCCCCCCCCCCCCEEEECHHHHHHCCCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure
MRISNGSGGDNVFTSGRFRRRGAATPLISLDFIVENGQGEILLGQRLNRPAQGYWFVPGG
CCCCCCCCCCCCEECCCCCCCCCCCCEEEEEEEEECCCCCEEECCCCCCCCCCEEECCCC
RVCKDETLEAAFARLTQAELGVRLPLAAGTFYGVWQHFYDDNFSGEDFSTHYIVLGFRLR
CCCCHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEE
VAESDLRLPDAQHGSYRWLTPEQLLASDNVHENSRAYFSPDAPAVGL
EECCCCCCCCCCCCCEEEECHHHHHHCCCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-mannose; H2O [C]

Specific reaction: GDP-mannose + H2O = GDP + mannose [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]