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The map label for this gene is rfbI

Identifier: 16765423

GI number: 16765423

Start: 2174120

End: 2175112

Strand: Reverse

Name: rfbI

Synonym: STM2093

Alternate gene names: 16765423

Gene position: 2175112-2174120 (Counterclockwise)

Preceding gene: 16765424

Following gene: 16765422

Centisome position: 44.78

GC content: 40.48

Gene sequence:

>993_bases
GTGTCTCATATTATTAAGATTTTTCCATCAAATATTGAATTTTCCGGTAGAGAGGATGAATCAATCCTCGATGCTGCGCT
ATCGGCTGGTATCCATCTTGAACATAGCTGCAAAGCGGGTGATTGTGGTATCTGTGAGTCCGATTTGTTGGCGGGAGAAG
TTGTTGACTCCAAAGGTAATATTTTTGGACAGGGTGATAAAATACTAACCTGCTGCTGTAAACCTAAAACCGCCCTTGAG
CTAAATGCGCATTTTTTTCCTGAACTAGCTGGACAGACAAAAAAAATTGTCCCATGCAAGGTAAATAGTGCTGTACTGGT
TTCAGGCGATGTTATGACTTTGAAGTTACGCACACCACCAACAGCAAAAATTGGCTTCCTTCCAGGGCAGTATATCAATT
TACATTATAAAGGTGTAACTCGCAGTTATTCTATCGCTAATAGTGATGAGTCGAATGGTATTGAGTTGCATGTAAGGAAT
GTTCCCAATGGTCAGATGAGTTCGCTCATTTTTGGGGAGTTACAAGAAAATACTCTTATGCGCATTGAAGGGCCTTGCGG
AACATTTTTTATTCGTGAAAGTGACAGACCTATAATCTTCCTTGCAGGCGGTACTGGATTCGCTCCAGTTAAATCAATGG
TTGAGCATCTCATTCAGGGAAAATGTCGTCGTGAGATCTACATTTACTGGGGAATGCAATATAGTAAAGATTTTTACTCT
GCATTACCGCAGCAGTGGAGTGAACAGCACGACAACGTTCATTATATCCCTGTTGTTTCTGGTGATGACGCCGAATGGGG
GGGAAGAAAGGGATTTGTCCATCATGCCGTGATGGATGATTTTGATTCTCTAGAGTTCTTCGATATATATGCATGTGGTT
CACCTGTGATGATCGATGCCAGTAAAAAGGACTTTATGATGAAAAATCTCTCTGTAGAACATTTCTATTCTGATGCATTT
ACCGCATCTAATAATATTGAGGATAATTTATGA

Upstream 100 bases:

>100_bases
CAATAGGTATTGAATGGCCTTTTTCTCAGCTGCCTGAGCTTTCAGCAAAAGATGCTGCAGCACCTTTACTGGATCAAGCC
TTGTTAACAGAGTAAGCATC

Downstream 100 bases:

>100_bases
AAGCGGTCATCCTGGCTGGTGGACTTGGTACCAGACTAAGTGAAGAAACAATTGTAAAACCAAAACCGATGGTAGAAATT
GGTGGCAAGCCTATTCTTTG

Product: CDP-6-deoxy-delta-3,4-glucoseen reductase

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSHIIKIFPSNIEFSGREDESILDAALSAGIHLEHSCKAGDCGICESDLLAGEVVDSKGNIFGQGDKILTCCCKPKTALE
LNAHFFPELAGQTKKIVPCKVNSAVLVSGDVMTLKLRTPPTAKIGFLPGQYINLHYKGVTRSYSIANSDESNGIELHVRN
VPNGQMSSLIFGELQENTLMRIEGPCGTFFIRESDRPIIFLAGGTGFAPVKSMVEHLIQGKCRREIYIYWGMQYSKDFYS
ALPQQWSEQHDNVHYIPVVSGDDAEWGGRKGFVHHAVMDDFDSLEFFDIYACGSPVMIDASKKDFMMKNLSVEHFYSDAF
TASNNIEDNL

Sequences:

>Translated_330_residues
MSHIIKIFPSNIEFSGREDESILDAALSAGIHLEHSCKAGDCGICESDLLAGEVVDSKGNIFGQGDKILTCCCKPKTALE
LNAHFFPELAGQTKKIVPCKVNSAVLVSGDVMTLKLRTPPTAKIGFLPGQYINLHYKGVTRSYSIANSDESNGIELHVRN
VPNGQMSSLIFGELQENTLMRIEGPCGTFFIRESDRPIIFLAGGTGFAPVKSMVEHLIQGKCRREIYIYWGMQYSKDFYS
ALPQQWSEQHDNVHYIPVVSGDDAEWGGRKGFVHHAVMDDFDSLEFFDIYACGSPVMIDASKKDFMMKNLSVEHFYSDAF
TASNNIEDNL
>Mature_329_residues
SHIIKIFPSNIEFSGREDESILDAALSAGIHLEHSCKAGDCGICESDLLAGEVVDSKGNIFGQGDKILTCCCKPKTALEL
NAHFFPELAGQTKKIVPCKVNSAVLVSGDVMTLKLRTPPTAKIGFLPGQYINLHYKGVTRSYSIANSDESNGIELHVRNV
PNGQMSSLIFGELQENTLMRIEGPCGTFFIRESDRPIIFLAGGTGFAPVKSMVEHLIQGKCRREIYIYWGMQYSKDFYSA
LPQQWSEQHDNVHYIPVVSGDDAEWGGRKGFVHHAVMDDFDSLEFFDIYACGSPVMIDASKKDFMMKNLSVEHFYSDAFT
ASNNIEDNL

Specific function: Catalyzes The Reduction Of Soluble Flavins By Reduced Pyridine Nucleotides. Seems To Reduces The Complexed Fe(3+) Iron Of Siderophores To Fe(2+), Thus Releasing It From The Chelator. [C]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain

Homologues:

Organism=Escherichia coli, GI2367314, Length=229, Percent_Identity=28.82096069869, Blast_Score=94, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFBI_SALTY (P26395)

Other databases:

- EMBL:   X56793
- EMBL:   AE006468
- PIR:   S15303
- RefSeq:   NP_461038.1
- ProteinModelPortal:   P26395
- SMR:   P26395
- PRIDE:   P26395
- GeneID:   1253614
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2093
- NMPDR:   fig|99287.1.peg.2018
- HOGENOM:   HBG680293
- OMA:   FCCANAS
- ProtClustDB:   PRK07609
- BioCyc:   STYP99287:STM2093-MONOMER
- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR00371
- PRINTS:   PR00410

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1; SSF54292 Ferredoxin; SSF63380 Riboflavin_synthase_like_b-brl

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 36583; Mature: 36452

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00197 2FE2S_FER_1; PS51085 2FE2S_FER_2; PS51384 FAD_FR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHIIKIFPSNIEFSGREDESILDAALSAGIHLEHSCKAGDCGICESDLLAGEVVDSKGN
CCCEEEEECCCEEECCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCEEECCCCC
IFGQGDKILTCCCKPKTALELNAHFFPELAGQTKKIVPCKVNSAVLVSGDVMTLKLRTPP
EEECCCEEEEEECCCCCEEEECCEECHHHCCCCCEEEEEECCCEEEEECCEEEEEECCCC
TAKIGFLPGQYINLHYKGVTRSYSIANSDESNGIELHVRNVPNGQMSSLIFGELQENTLM
CCEEECCCCCEEEEEEECCEEEEEECCCCCCCCEEEEEEECCCCHHHHHEEEECCCCEEE
RIEGPCGTFFIRESDRPIIFLAGGTGFAPVKSMVEHLIQGKCRREIYIYWGMQYSKDFYS
EEECCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHH
ALPQQWSEQHDNVHYIPVVSGDDAEWGGRKGFVHHAVMDDFDSLEFFDIYACGSPVMIDA
HHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEHHHHCCCCCCEEEEEEECCCCEEEEC
SKKDFMMKNLSVEHFYSDAFTASNNIEDNL
CCCHHHHHCCCHHHEECCCEECCCCCCCCC
>Mature Secondary Structure 
SHIIKIFPSNIEFSGREDESILDAALSAGIHLEHSCKAGDCGICESDLLAGEVVDSKGN
CCEEEEECCCEEECCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCEEECCCCC
IFGQGDKILTCCCKPKTALELNAHFFPELAGQTKKIVPCKVNSAVLVSGDVMTLKLRTPP
EEECCCEEEEEECCCCCEEEECCEECHHHCCCCCEEEEEECCCEEEEECCEEEEEECCCC
TAKIGFLPGQYINLHYKGVTRSYSIANSDESNGIELHVRNVPNGQMSSLIFGELQENTLM
CCEEECCCCCEEEEEEECCEEEEEECCCCCCCCEEEEEEECCCCHHHHHEEEECCCCEEE
RIEGPCGTFFIRESDRPIIFLAGGTGFAPVKSMVEHLIQGKCRREIYIYWGMQYSKDFYS
EEECCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHH
ALPQQWSEQHDNVHYIPVVSGDDAEWGGRKGFVHHAVMDDFDSLEFFDIYACGSPVMIDA
HHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEHHHHCCCCCCEEEEEEECCCCEEEEC
SKKDFMMKNLSVEHFYSDAFTASNNIEDNL
CCCHHHHHCCCHHHEECCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609