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The map label for this gene is rfbJ

Identifier: 16765419

GI number: 16765419

Start: 2169999

End: 2170898

Strand: Reverse

Name: rfbJ

Synonym: STM2089

Alternate gene names: 16765419

Gene position: 2170898-2169999 (Counterclockwise)

Preceding gene: 16765420

Following gene: 16765418

Centisome position: 44.69

GC content: 32.0

Gene sequence:

>900_bases
ATGACCTTTTTGAAAGAATATGTAATTGTCAGTGGGGCTTCCGGCTTTATTGGTAAGCATTTACTCGAAGCGCTAAAAAA
ATCGGGGATTTCAGTTGTCGCAATCACTCGAGATGTAATAAAAAATAATAGTAATGCATTAGCTAATGTTAGATGGTGCA
GTTGGGATAATATCGAATTATTAGTCGAGGAGTTATCAATTGATTCTGCATTAATTGGTATCATTCATTTGGCAACAGAA
TATGGGCATAAAACATCATCTCTCATAAATATTGAAGATGCAAATGTTATAAAACCATTAAAGCTTCTTGATTTGGCAAT
AAAATATCGGGCGGATATCTTTTTAAATACAGATAGTTTTTTTGCCAAGAAAGATTTTAATTATCAACATATGCGGCCTT
ATATAATTACTAAAAGACACTTTGATGAAATTGGGCATTATTATGCTAATATGCATGACATTTCATTTGTAAACATGCGA
TTAGAGCATGTATATGGGCCTGGGGATGGTGAAAATAAATTTATTCCATACATTATCGACTGCTTAAATAAAAAACAGAG
TTGCGTGAAATGTACAACAGGCGAACAGATAAGAGACTTTATTTTTGTAGATGATGTGGTAAATGCTTATTTAACTATAT
TAGAAAATAGAAAAGAAGTACCTTCATATACTGAGTATCAAGTTGGAACTGGTGCTGGGGTAAGTTTGAAAGATTTTCTG
GTTTATTTGCAAAATACTATGATGCCAGGTTCATCGAGTATATTTGAATTTGGTGCGATAGAGCAAAGAGATAATGAAAT
AATGTTCTCTGTAGCAAATAATAAAAATTTAAAAGCAATGGGCTGGAAACCAAATTTCGATTATAAAAAAGGAATTGAAG
AACTACTGAAACGGTTATGA

Upstream 100 bases:

>100_bases
TCCAGGCTTGACTACAGAGCATTTAGATTATGTAGTTAGCAAGTTTGAAGAGTTCTTTGGTTTGAATTTCTAATTCAATT
TATTCTATCTGGTGATTGCG

Downstream 100 bases:

>100_bases
GATTTTCATGATCTTTTAATAAATAAATCGTTAACAAATTAGTCGCGTTATGTTGTAAAAACTAAGTCGTTTAATTGCAT
AGTGAAAGTTCAATTGTTAA

Product: CDP-abequose synthase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: O4 antigen

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTFLKEYVIVSGASGFIGKHLLEALKKSGISVVAITRDVIKNNSNALANVRWCSWDNIELLVEELSIDSALIGIIHLATE
YGHKTSSLINIEDANVIKPLKLLDLAIKYRADIFLNTDSFFAKKDFNYQHMRPYIITKRHFDEIGHYYANMHDISFVNMR
LEHVYGPGDGENKFIPYIIDCLNKKQSCVKCTTGEQIRDFIFVDDVVNAYLTILENRKEVPSYTEYQVGTGAGVSLKDFL
VYLQNTMMPGSSSIFEFGAIEQRDNEIMFSVANNKNLKAMGWKPNFDYKKGIEELLKRL

Sequences:

>Translated_299_residues
MTFLKEYVIVSGASGFIGKHLLEALKKSGISVVAITRDVIKNNSNALANVRWCSWDNIELLVEELSIDSALIGIIHLATE
YGHKTSSLINIEDANVIKPLKLLDLAIKYRADIFLNTDSFFAKKDFNYQHMRPYIITKRHFDEIGHYYANMHDISFVNMR
LEHVYGPGDGENKFIPYIIDCLNKKQSCVKCTTGEQIRDFIFVDDVVNAYLTILENRKEVPSYTEYQVGTGAGVSLKDFL
VYLQNTMMPGSSSIFEFGAIEQRDNEIMFSVANNKNLKAMGWKPNFDYKKGIEELLKRL
>Mature_298_residues
TFLKEYVIVSGASGFIGKHLLEALKKSGISVVAITRDVIKNNSNALANVRWCSWDNIELLVEELSIDSALIGIIHLATEY
GHKTSSLINIEDANVIKPLKLLDLAIKYRADIFLNTDSFFAKKDFNYQHMRPYIITKRHFDEIGHYYANMHDISFVNMRL
EHVYGPGDGENKFIPYIIDCLNKKQSCVKCTTGEQIRDFIFVDDVVNAYLTILENRKEVPSYTEYQVGTGAGVSLKDFLV
YLQNTMMPGSSSIFEFGAIEQRDNEIMFSVANNKNLKAMGWKPNFDYKKGIEELLKRL

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI7657641, Length=321, Percent_Identity=24.9221183800623, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFBJ_SALTY (P0A1P4)

Other databases:

- EMBL:   X56793
- EMBL:   AE006468
- PIR:   S15307
- RefSeq:   NP_461034.1
- ProteinModelPortal:   P0A1P4
- PRIDE:   P0A1P4
- GeneID:   1253610
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2089
- NMPDR:   fig|99287.1.peg.2014
- HOGENOM:   HBG362914
- OMA:   IHTATCY
- ProtClustDB:   CLSK894750
- BioCyc:   MetaCyc:MONOMER-13793
- BioCyc:   STYP99287:STM2089-MONOMER
- InterPro:   IPR001509
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF01370 Epimerase

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 34107; Mature: 33975

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: ACT_SITE 134-134 BINDING 117-117

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFLKEYVIVSGASGFIGKHLLEALKKSGISVVAITRDVIKNNSNALANVRWCSWDNIEL
CCHHHCCCEEECCCHHHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCEEEEEEECCCCHHH
LVEELSIDSALIGIIHLATEYGHKTSSLINIEDANVIKPLKLLDLAIKYRADIFLNTDSF
HHHHHCCCHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHEEEEEEEECCCC
FAKKDFNYQHMRPYIITKRHFDEIGHYYANMHDISFVNMRLEHVYGPGDGENKFIPYIID
EEECCCCHHHCCCEEEEHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCCCCHHHHHH
CLNKKQSCVKCTTGEQIRDFIFVDDVVNAYLTILENRKEVPSYTEYQVGTGAGVSLKDFL
HHCCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCHHHHH
VYLQNTMMPGSSSIFEFGAIEQRDNEIMFSVANNKNLKAMGWKPNFDYKKGIEELLKRL
HHHHHHCCCCCCCCEECCCCCCCCCEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHC
>Mature Secondary Structure 
TFLKEYVIVSGASGFIGKHLLEALKKSGISVVAITRDVIKNNSNALANVRWCSWDNIEL
CHHHCCCEEECCCHHHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCEEEEEEECCCCHHH
LVEELSIDSALIGIIHLATEYGHKTSSLINIEDANVIKPLKLLDLAIKYRADIFLNTDSF
HHHHHCCCHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHEEEEEEEECCCC
FAKKDFNYQHMRPYIITKRHFDEIGHYYANMHDISFVNMRLEHVYGPGDGENKFIPYIID
EEECCCCHHHCCCEEEEHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCCCCHHHHHH
CLNKKQSCVKCTTGEQIRDFIFVDDVVNAYLTILENRKEVPSYTEYQVGTGAGVSLKDFL
HHCCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCHHHHH
VYLQNTMMPGSSSIFEFGAIEQRDNEIMFSVANNKNLKAMGWKPNFDYKKGIEELLKRL
HHHHHHCCCCCCCCEECCCCCCCCCEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2793832; 1710759; 11677609