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The map label for this gene is phsA

Identifier: 16765395

GI number: 16765395

Start: 2138675

End: 2140951

Strand: Reverse

Name: phsA

Synonym: STM2065

Alternate gene names: 16765395

Gene position: 2140951-2138675 (Counterclockwise)

Preceding gene: 16765398

Following gene: 16765394

Centisome position: 44.08

GC content: 55.47

Gene sequence:

>2277_bases
ATGAGCATTAGTCGTCGTTCTTTTTTGCAGGGGGTAGGCATCGGCTGCTCTGCCTGCGCGCTGGGCGCTTTTCCGCCCGG
GGCGCTGGCGCGCAACCCGATTGCCGGCATTAACGGTAAAACCACGCTAACGCCAAGCTTGTGCGAAATGTGTTCCTTTC
GTTGCCCTATTCAGGCGCAGGTAGTCAATAACAAGACCGTTTTTATCCAGGGCAATCCTTCTGCGCCGCAGCAGGGGACG
CGCATATGCGCCAGGGGCGGGAGCGGCGTCAGCCTGGTCAATGACCCGCAACGGATTGTAAAACCTATGAAACGCACCGG
GCCACGCGGCGACGGTGAATGGCAGGTGATTAGCTGGCAACAGGCTTACCAGGAAATCGCGGCGAAAATGAATGCCATCA
AAGCGCAGCATGGCCCCGAGAGCGTGGCTTTCTCTTCCAAATCGGGCTCGCTCTCCAGCCATCTTTTCCATCTGGCTACG
GCCTTTGGTTCGCCTAATACCTTTACGCACGCCTCAACATGCCCTGCCGGGAAAGCCATTGCGGCAAAAGTGATGATGGG
CGGCGATCTGGCGATGGATATCGCTAACACGCGCTATCTGGTTTCGTTTGGCCACAATTTGTATGAAGGGATTGAAGTTG
CCGATACCCATGAGTTAATGACCGCGCAGGAGAAGGGGGCCAAAATGGTGAGCTTCGATCCGCGTTTGTCGATATTTTCC
AGCAAGGCGGATGAGTGGCACGCTATTCGTCCCGGGGGGGATTTAGCGGTTCTGCTGGCGATGTGCCACGTCATGATTGA
TGAACAGCTCTACGATGCGTCTTTTGTTGAGCGTTATACCAGCGGATTTGAACAGTTAGCACAGGCGGTAAAAGAGACGA
CGCCGGAATGGGCCGCCGCGCAGGCCGATGTCCCAGCCGACGTTATTGTCCGGGTGACACGCGAACTGGCTGCCTGCGCG
CCTCACGCTATTGTCAGTCCTGGTCATCGCGCGACGTTCTCGCAGGAAGAGATCGATATGCGGCGTATGATTTTTACGCT
TAATGTGCTGCTCGGTAATATTGAGCGCGAAGGCGGGCTATATCAGAAAAAAAACGCGTCTGTTTACAATAAACTGGCCG
GAGAAAAGGTCGCGCCAACGCTGGCGAAACTCAACATTAAAAATATGCCGAAACCGACGGCGCAACGCATCGATTTGGTC
GCACCGCAGTTTAAATATATCGCCGCTGGCGGCGGCGTGGTGCAAAGCATTATTGACTCGGCGTTAACCCAGAAGCCTTA
CCCGATAAAGGCGTGGATTATGTCGCGGCATAATCCTTTCCAGACCGTCACCTGTCGTTCGGACCTGGTAAAAACCGTTG
AGCAACTGGATCTGGTGGTCAGCTGCGATGTCTATTTGAGCGAGAGCGCGGCATATGCCGACTATCTGCTGCCGGAATGC
ACCTATCTCGAACGGGACGAAGAGGTATCCGATATGTCGGGACTGCACCCGGCTTACGCTCTGCGCCAACAGGTCGTAGA
GCCGATTGGCGAGGCGCGTCCGAGTTGGCAAATCTGGAAAGAACTTGGCGAGCAGTTGGGATTAGGGCAGTACTATCCGT
GGCAGGATATGCAGACGCGCCAACTCTATCAGTTGAACGGCGACCATGCCTTAGCGAAGGAACTGCGACAGAAAGGGTAT
CTCGAATGGGGCGTTCCGCTGCTATTACGCGAACCAGAATCCGTTCGTCAGTTTACGGCGCGTTACCCCGGCGCTATCGC
GACGGACAGTGACAACACCTATGGCGAACAGCTTCGCTTCAAATCGCCCTCCGGCAAAATCGAACTTTATTCCGCAACCC
TGGAGGAATTGCTCCCTGGCTACGGCGTTCCGCGCGTTCGTGACTTTGCGCTGAAAAAAGAGAATGAGCTTTACTTCATT
CAGGGCAAGGTGGCCGTGCATACCAATGGCGCGACGCAGTACGTACCTTTACTCAGCGAGCTAATGTGGGATAACGCGGT
CTGGGTTCATCCGCAAACGGCGCAAGAAAAAGGCATTAAGACCGGCGATGAGATCTGGCTGGAAAATGCCACGGGTAAAG
AGAAAGGTAAGGCGCTGGTGACGCCCGGTATCCGCCCGGACACGCTTTTTGTCTATATGGGATTTGGCGCTAAAGCTGGG
GCCAAAACGGCGGCGACGACACACGGTATCCACTGCGGAAATTTACTGCCGCACGTGACGAGTCCGGTATCCGGTACGGT
AGTGCATACCGCAGGCGTGACGCTGAGCCGGGCATGA

Upstream 100 bases:

>100_bases
CATGCTCATTTTAACAACTATTTCAAATTGTGATTGAAGCCGACATTTCATAATACAAATTAAATATAATCTTCAGCTAT
ATCTAATAACAGGAGGTTAT

Downstream 100 bases:

>100_bases
GGGAGGAGGGAACCATGAATCATTTAACGAATCAGTACGTCATGCTGCATGATGAAAAACGTTGTATCGGCTGCCAGGCG
TGTACCGTTGCCTGCAAAGT

Product: thiosulfate reductase precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 758; Mature: 757

Protein sequence:

>758_residues
MSISRRSFLQGVGIGCSACALGAFPPGALARNPIAGINGKTTLTPSLCEMCSFRCPIQAQVVNNKTVFIQGNPSAPQQGT
RICARGGSGVSLVNDPQRIVKPMKRTGPRGDGEWQVISWQQAYQEIAAKMNAIKAQHGPESVAFSSKSGSLSSHLFHLAT
AFGSPNTFTHASTCPAGKAIAAKVMMGGDLAMDIANTRYLVSFGHNLYEGIEVADTHELMTAQEKGAKMVSFDPRLSIFS
SKADEWHAIRPGGDLAVLLAMCHVMIDEQLYDASFVERYTSGFEQLAQAVKETTPEWAAAQADVPADVIVRVTRELAACA
PHAIVSPGHRATFSQEEIDMRRMIFTLNVLLGNIEREGGLYQKKNASVYNKLAGEKVAPTLAKLNIKNMPKPTAQRIDLV
APQFKYIAAGGGVVQSIIDSALTQKPYPIKAWIMSRHNPFQTVTCRSDLVKTVEQLDLVVSCDVYLSESAAYADYLLPEC
TYLERDEEVSDMSGLHPAYALRQQVVEPIGEARPSWQIWKELGEQLGLGQYYPWQDMQTRQLYQLNGDHALAKELRQKGY
LEWGVPLLLREPESVRQFTARYPGAIATDSDNTYGEQLRFKSPSGKIELYSATLEELLPGYGVPRVRDFALKKENELYFI
QGKVAVHTNGATQYVPLLSELMWDNAVWVHPQTAQEKGIKTGDEIWLENATGKEKGKALVTPGIRPDTLFVYMGFGAKAG
AKTAATTHGIHCGNLLPHVTSPVSGTVVHTAGVTLSRA

Sequences:

>Translated_758_residues
MSISRRSFLQGVGIGCSACALGAFPPGALARNPIAGINGKTTLTPSLCEMCSFRCPIQAQVVNNKTVFIQGNPSAPQQGT
RICARGGSGVSLVNDPQRIVKPMKRTGPRGDGEWQVISWQQAYQEIAAKMNAIKAQHGPESVAFSSKSGSLSSHLFHLAT
AFGSPNTFTHASTCPAGKAIAAKVMMGGDLAMDIANTRYLVSFGHNLYEGIEVADTHELMTAQEKGAKMVSFDPRLSIFS
SKADEWHAIRPGGDLAVLLAMCHVMIDEQLYDASFVERYTSGFEQLAQAVKETTPEWAAAQADVPADVIVRVTRELAACA
PHAIVSPGHRATFSQEEIDMRRMIFTLNVLLGNIEREGGLYQKKNASVYNKLAGEKVAPTLAKLNIKNMPKPTAQRIDLV
APQFKYIAAGGGVVQSIIDSALTQKPYPIKAWIMSRHNPFQTVTCRSDLVKTVEQLDLVVSCDVYLSESAAYADYLLPEC
TYLERDEEVSDMSGLHPAYALRQQVVEPIGEARPSWQIWKELGEQLGLGQYYPWQDMQTRQLYQLNGDHALAKELRQKGY
LEWGVPLLLREPESVRQFTARYPGAIATDSDNTYGEQLRFKSPSGKIELYSATLEELLPGYGVPRVRDFALKKENELYFI
QGKVAVHTNGATQYVPLLSELMWDNAVWVHPQTAQEKGIKTGDEIWLENATGKEKGKALVTPGIRPDTLFVYMGFGAKAG
AKTAATTHGIHCGNLLPHVTSPVSGTVVHTAGVTLSRA
>Mature_757_residues
SISRRSFLQGVGIGCSACALGAFPPGALARNPIAGINGKTTLTPSLCEMCSFRCPIQAQVVNNKTVFIQGNPSAPQQGTR
ICARGGSGVSLVNDPQRIVKPMKRTGPRGDGEWQVISWQQAYQEIAAKMNAIKAQHGPESVAFSSKSGSLSSHLFHLATA
FGSPNTFTHASTCPAGKAIAAKVMMGGDLAMDIANTRYLVSFGHNLYEGIEVADTHELMTAQEKGAKMVSFDPRLSIFSS
KADEWHAIRPGGDLAVLLAMCHVMIDEQLYDASFVERYTSGFEQLAQAVKETTPEWAAAQADVPADVIVRVTRELAACAP
HAIVSPGHRATFSQEEIDMRRMIFTLNVLLGNIEREGGLYQKKNASVYNKLAGEKVAPTLAKLNIKNMPKPTAQRIDLVA
PQFKYIAAGGGVVQSIIDSALTQKPYPIKAWIMSRHNPFQTVTCRSDLVKTVEQLDLVVSCDVYLSESAAYADYLLPECT
YLERDEEVSDMSGLHPAYALRQQVVEPIGEARPSWQIWKELGEQLGLGQYYPWQDMQTRQLYQLNGDHALAKELRQKGYL
EWGVPLLLREPESVRQFTARYPGAIATDSDNTYGEQLRFKSPSGKIELYSATLEELLPGYGVPRVRDFALKKENELYFIQ
GKVAVHTNGATQYVPLLSELMWDNAVWVHPQTAQEKGIKTGDEIWLENATGKEKGKALVTPGIRPDTLFVYMGFGAKAGA
KTAATTHGIHCGNLLPHVTSPVSGTVVHTAGVTLSRA

Specific function: Oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential)

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family

Homologues:

Organism=Escherichia coli, GI1787870, Length=835, Percent_Identity=26.4670658682635, Blast_Score=198, Evalue=1e-51,
Organism=Escherichia coli, GI87081797, Length=791, Percent_Identity=25.5372945638432, Blast_Score=185, Evalue=1e-47,
Organism=Escherichia coli, GI171474008, Length=828, Percent_Identity=25.1207729468599, Blast_Score=176, Evalue=6e-45,
Organism=Escherichia coli, GI3868721, Length=730, Percent_Identity=24.3835616438356, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI1787231, Length=717, Percent_Identity=22.1757322175732, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI87081994, Length=795, Percent_Identity=22.5157232704403, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1788534, Length=822, Percent_Identity=20.8029197080292, Blast_Score=73, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHSA_SALTY (P37600)

Other databases:

- EMBL:   L32188
- EMBL:   AE006468
- EMBL:   L31538
- PIR:   A57143
- RefSeq:   NP_461010.1
- ProteinModelPortal:   P37600
- PRIDE:   P37600
- GeneID:   1253586
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2065
- HOGENOM:   HBG575272
- OMA:   GPETVAF
- ProtClustDB:   PRK15488
- BioCyc:   MetaCyc:MONOMER-12529
- BioCyc:   STYP99287:STM2065-MONOMER
- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311
- Gene3D:   G3DSA:2.40.40.20
- SMART:   SM00926
- TIGRFAMs:   TIGR01409

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding; SSF50692 Asp_decarb_fold

EC number: 1.8.99.- [C]

Molecular weight: Translated: 82800; Mature: 82669

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1; PS00490 MOLYBDOPTERIN_PROK_2; PS00932 MOLYBDOPTERIN_PROK_3; PS51318 TAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISRRSFLQGVGIGCSACALGAFPPGALARNPIAGINGKTTLTPSLCEMCSFRCPIQAQ
CCCCHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHCCCCCEEEE
VVNNKTVFIQGNPSAPQQGTRICARGGSGVSLVNDPQRIVKPMKRTGPRGDGEWQVISWQ
EECCEEEEEECCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEHH
QAYQEIAAKMNAIKAQHGPESVAFSSKSGSLSSHLFHLATAFGSPNTFTHASTCPAGKAI
HHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
AAKVMMGGDLAMDIANTRYLVSFGHNLYEGIEVADTHELMTAQEKGAKMVSFDPRLSIFS
HHHHEECCCEEEECCCCEEEEECCCHHHHCCCCCCHHHHHHHHHHCCEEEECCCCHHHHC
SKADEWHAIRPGGDLAVLLAMCHVMIDEQLYDASFVERYTSGFEQLAQAVKETTPEWAAA
CCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHC
QADVPADVIVRVTRELAACAPHAIVSPGHRATFSQEEIDMRRMIFTLNVLLGNIEREGGL
CCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
YQKKNASVYNKLAGEKVAPTLAKLNIKNMPKPTAQRIDLVAPQFKYIAAGGGVVQSIIDS
CCCCCCHHHHHHCCCHHCCHHHHCCCCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHH
ALTQKPYPIKAWIMSRHNPFQTVTCRSDLVKTVEQLDLVVSCDVYLSESAAYADYLLPEC
HHHCCCCCEEEEEECCCCCCEEEECHHHHHHHHHHHCEEEEECEEECCCHHHHHHHCCCC
TYLERDEEVSDMSGLHPAYALRQQVVEPIGEARPSWQIWKELGEQLGLGQYYPWQDMQTR
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCH
QLYQLNGDHALAKELRQKGYLEWGVPLLLREPESVRQFTARYPGAIATDSDNTYGEQLRF
HHHHCCCCHHHHHHHHHCCCEECCCCEEEECCHHHHHHHHHCCCCEECCCCCCCCCCEEE
KSPSGKIELYSATLEELLPGYGVPRVRDFALKKENELYFIQGKVAVHTNGATQYVPLLSE
ECCCCCEEEHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEEEEEEEECCCHHHHHHHHH
LMWDNAVWVHPQTAQEKGIKTGDEIWLENATGKEKGKALVTPGIRPDTLFVYMGFGAKAG
HHHCCEEEECCCHHHHCCCCCCCCEEEECCCCCCCCCEEECCCCCCCEEEEEECCCCCCC
AKTAATTHGIHCGNLLPHVTSPVSGTVVHTAGVTLSRA
CCCCHHCCCCCCCCCCCCCCCCCCCEEEEECCEEEECC
>Mature Secondary Structure 
SISRRSFLQGVGIGCSACALGAFPPGALARNPIAGINGKTTLTPSLCEMCSFRCPIQAQ
CCCHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHCCCCCEEEE
VVNNKTVFIQGNPSAPQQGTRICARGGSGVSLVNDPQRIVKPMKRTGPRGDGEWQVISWQ
EECCEEEEEECCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEHH
QAYQEIAAKMNAIKAQHGPESVAFSSKSGSLSSHLFHLATAFGSPNTFTHASTCPAGKAI
HHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
AAKVMMGGDLAMDIANTRYLVSFGHNLYEGIEVADTHELMTAQEKGAKMVSFDPRLSIFS
HHHHEECCCEEEECCCCEEEEECCCHHHHCCCCCCHHHHHHHHHHCCEEEECCCCHHHHC
SKADEWHAIRPGGDLAVLLAMCHVMIDEQLYDASFVERYTSGFEQLAQAVKETTPEWAAA
CCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHC
QADVPADVIVRVTRELAACAPHAIVSPGHRATFSQEEIDMRRMIFTLNVLLGNIEREGGL
CCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
YQKKNASVYNKLAGEKVAPTLAKLNIKNMPKPTAQRIDLVAPQFKYIAAGGGVVQSIIDS
CCCCCCHHHHHHCCCHHCCHHHHCCCCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHH
ALTQKPYPIKAWIMSRHNPFQTVTCRSDLVKTVEQLDLVVSCDVYLSESAAYADYLLPEC
HHHCCCCCEEEEEECCCCCCEEEECHHHHHHHHHHHCEEEEECEEECCCHHHHHHHCCCC
TYLERDEEVSDMSGLHPAYALRQQVVEPIGEARPSWQIWKELGEQLGLGQYYPWQDMQTR
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCH
QLYQLNGDHALAKELRQKGYLEWGVPLLLREPESVRQFTARYPGAIATDSDNTYGEQLRF
HHHHCCCCHHHHHHHHHCCCEECCCCEEEECCHHHHHHHHHCCCCEECCCCCCCCCCEEE
KSPSGKIELYSATLEELLPGYGVPRVRDFALKKENELYFIQGKVAVHTNGATQYVPLLSE
ECCCCCEEEHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEEEEEEEECCCHHHHHHHHH
LMWDNAVWVHPQTAQEKGIKTGDEIWLENATGKEKGKALVTPGIRPDTLFVYMGFGAKAG
HHHCCEEEECCCHHHHCCCCCCCCEEEECCCCCCCCCEEECCCCCCCEEEEEECCCCCCC
AKTAATTHGIHCGNLLPHVTSPVSGTVVHTAGVTLSRA
CCCCHHCCCCCCCCCCCCCCCCCCCEEEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7751291; 11677609; 7737516