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The map label for this gene is cbiL

Identifier: 16765354

GI number: 16765354

Start: 2103704

End: 2104417

Strand: Reverse

Name: cbiL

Synonym: STM2024

Alternate gene names: 16765354

Gene position: 2104417-2103704 (Counterclockwise)

Preceding gene: 16765355

Following gene: 161353594

Centisome position: 43.32

GC content: 57.84

Gene sequence:

>714_bases
ATGAACGGTAAATTATACGCATTAAGTACCGGGCCAGGCGCGCCCGACCTGATAACCGTCCGCGCAGCGCGGATTCTTGG
CTCGCTGGACATTCTGTATGCGCCCGCCGGTCGTAAGGGGGGCGATAGTCTGGCGCTGTCGATTGTGCGCGACTATCTCG
GCGAGCAGACGGAAGTACGTTGTTGCCATTTCCCTATGAGCGCTGACGGCGCCGAGAAAGAAGCGGTCTGGAACGAGGTT
GCCGCCGCGCTTACCGCAGAGGTGGAAGCGGGTAAACAGGTCGGTTTTATTACCCTGGGCGATGCGATGCTATTCAGTAC
CTGGATTTTTTTACTCCAGCGAATCGGCTGCCCGGAGTGGCTGGAGATTGTCCCCGGCGTCACGTCCTTCGCCGCGATAG
CCGCCCGCGCGAAAATGCCGCTCGCCATAGAACGGCAATCGCTGGCGGTTATTTCTTGTACCGCGCCGGAAGCGGAAATA
GCCCAGGCGCTACAACAGCATGATAGCCTGGTGTTGATGAAAGTGTATGGCCGGTTCGCCCGTATTAAAGCTTTGCTGGC
GCAGGCTGGCCTGCTGGAATGCGCCTTAATGATGTCCGAGGCCACACTGCCCGGCGAGCAGTGCTGGCGCCATCTCCACG
AGGTCAACGATGATCGACCGTTGCCCTATTTTTCGACCATTCTGGTCAATAAACAGTGGGAGTATGCAGAATGA

Upstream 100 bases:

>100_bases
CGACGCCGTGGCTGAGCGGGCTGGGAGAAAACCCGGCTATCCGGGCGATGTTTGTCGCGCATTTGCACCAGGCGCTGAAC
ATGGCGGTAGAGGAGGCGGC

Downstream 100 bases:

>100_bases
AACTTGAACAGCAGCTTCGACAGTTGTCTTTCAGTGGACTGGCTGCAGCGCTATTGCTGATGGTTGTCCCGCAACAGGCG
TTTGCGATGCATATCATGGA

Product: cobalt-precorrin-2 C(20)-methyltransferase

Products: NA

Alternate protein names: S-adenosyl-L-methionine--cobalt-precorrin-2 methyltransferase

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MNGKLYALSTGPGAPDLITVRAARILGSLDILYAPAGRKGGDSLALSIVRDYLGEQTEVRCCHFPMSADGAEKEAVWNEV
AAALTAEVEAGKQVGFITLGDAMLFSTWIFLLQRIGCPEWLEIVPGVTSFAAIAARAKMPLAIERQSLAVISCTAPEAEI
AQALQQHDSLVLMKVYGRFARIKALLAQAGLLECALMMSEATLPGEQCWRHLHEVNDDRPLPYFSTILVNKQWEYAE

Sequences:

>Translated_237_residues
MNGKLYALSTGPGAPDLITVRAARILGSLDILYAPAGRKGGDSLALSIVRDYLGEQTEVRCCHFPMSADGAEKEAVWNEV
AAALTAEVEAGKQVGFITLGDAMLFSTWIFLLQRIGCPEWLEIVPGVTSFAAIAARAKMPLAIERQSLAVISCTAPEAEI
AQALQQHDSLVLMKVYGRFARIKALLAQAGLLECALMMSEATLPGEQCWRHLHEVNDDRPLPYFSTILVNKQWEYAE
>Mature_237_residues
MNGKLYALSTGPGAPDLITVRAARILGSLDILYAPAGRKGGDSLALSIVRDYLGEQTEVRCCHFPMSADGAEKEAVWNEV
AAALTAEVEAGKQVGFITLGDAMLFSTWIFLLQRIGCPEWLEIVPGVTSFAAIAARAKMPLAIERQSLAVISCTAPEAEI
AQALQQHDSLVLMKVYGRFARIKALLAQAGLLECALMMSEATLPGEQCWRHLHEVNDDRPLPYFSTILVNKQWEYAE

Specific function: Methylates cobalt-precorrin-2 at the C-20 position to produce cobalt-precorrin-3A in the anaerobic cobalamin biosynthesis pathway

COG id: COG2243

COG function: function code H; Precorrin-2 methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CBIL_SALTY (Q05593)

Other databases:

- EMBL:   L12006
- EMBL:   AE006468
- RefSeq:   NP_460969.1
- ProteinModelPortal:   Q05593
- SMR:   Q05593
- PRIDE:   Q05593
- GeneID:   1253545
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM2024
- NMPDR:   fig|99287.1.peg.1949
- HOGENOM:   HBG724388
- OMA:   VIMKVGR
- ProtClustDB:   PRK05576
- BioCyc:   MetaCyc:MONOMER-13216
- BioCyc:   STYP99287:STM2024-MONOMER
- BRENDA:   2.1.1.151
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR012382
- InterPro:   IPR006364
- InterPro:   IPR003043
- Gene3D:   G3DSA:3.40.1010.10
- PIRSF:   PIRSF036427
- TIGRFAMs:   TIGR01467

Pfam domain/function: PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: =2.1.1.151

Molecular weight: Translated: 25806; Mature: 25806

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00839 SUMT_1; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGKLYALSTGPGAPDLITVRAARILGSLDILYAPAGRKGGDSLALSIVRDYLGEQTEVR
CCCEEEEEECCCCCCCHHHHHHHHHHCCHHEEECCCCCCCCHHHHHHHHHHHHCCCCCEE
CCHFPMSADGAEKEAVWNEVAAALTAEVEAGKQVGFITLGDAMLFSTWIFLLQRIGCPEW
EEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHCCCHHH
LEIVPGVTSFAAIAARAKMPLAIERQSLAVISCTAPEAEIAQALQQHDSLVLMKVYGRFA
HHHHCCHHHHHHHHHHCCCCEEEECCCEEEEEECCCHHHHHHHHHHCCCEEHHHHHHHHH
RIKALLAQAGLLECALMMSEATLPGEQCWRHLHEVNDDRPLPYFSTILVNKQWEYAE
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHEEEECCCCCCC
>Mature Secondary Structure
MNGKLYALSTGPGAPDLITVRAARILGSLDILYAPAGRKGGDSLALSIVRDYLGEQTEVR
CCCEEEEEECCCCCCCHHHHHHHHHHCCHHEEECCCCCCCCHHHHHHHHHHHHCCCCCEE
CCHFPMSADGAEKEAVWNEVAAALTAEVEAGKQVGFITLGDAMLFSTWIFLLQRIGCPEW
EEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHCCCHHH
LEIVPGVTSFAAIAARAKMPLAIERQSLAVISCTAPEAEIAQALQQHDSLVLMKVYGRFA
HHHHCCHHHHHHHHHHCCCCEEEECCCEEEEEECCCHHHHHHHHHHCCCEEHHHHHHHHH
RIKALLAQAGLLECALMMSEATLPGEQCWRHLHEVNDDRPLPYFSTILVNKQWEYAE
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609