Click here to switch to the map view.

The map label for this gene is cobS [H]

Identifier: 16765347

GI number: 16765347

Start: 2098400

End: 2099143

Strand: Reverse

Name: cobS [H]

Synonym: STM2017

Alternate gene names: 16765347

Gene position: 2099143-2098400 (Counterclockwise)

Preceding gene: 16765348

Following gene: 16765346

Centisome position: 43.22

GC content: 56.45

Gene sequence:

>744_bases
ATGAGTAAGCTGTTTTGGGCCATGCTCGCTTTTATTAGCCGCTTGCCCGTGCCGTCACGCTGGTCGCAGGGACTGGATTT
CGAGCAGTATTCGCGTGGGATCGTGATGTTTCCCTTTATCGGGTTGATTCTGGGGGGCGTAAGCGGCCTGATTTTCATCC
TCCTGCAACCCTGGTGTGGTATTCCGCTAGCGGCGTTGTTCTGTATTCTGGCGCTGGCGCTGCTGACCGGTGGTTTTCAC
CTGGATGGGCTGGCCGATACCTGCGATGGCATTTTTTCCGCGCGCCGCCGTGAGCGAATGCTGGAGATTATGCGTGATAG
TCGTCTGGGAACCCATGGCGGGCTGGCGCTTATTTTTGTGCTACTGGCAAAAATTCTGGTGGTCAGCGAACTGGCGTTAC
GTGGAACGCCAATGTTGGCGGCGTTGGCGGCGGCCTGTGCGGCCGGACGCGGCAGCGCCGTTTTATTGATGTACCGTCAT
CGCTACGCCCGTGAAGAGGGGCTTGGCAATGTATTTATCGGTAAAGTCAGCGGACGGCAAACCTGCATTACTCTGGGATT
GGCGGTAATCGTCGCCACGGTATTACTGCCTGGTATGCAAGGGTTGGCCGCAATGGTGGTCACATGCGCGGCGATTTTCA
TCCTTGGCCAGTTGCTTAAACGTACGCTTGGCGGTCAAACCGGCGATACGCTGGGCGCGGCGATCGAACTTGGTGAATTG
ATCTTTCTGCTGGCTCTGTTATGA

Upstream 100 bases:

>100_bases
CGCGCCATTTTCGTGATATTGCCGGTCGGGTCAACCAACGACTGGCGGCAGCGGCGGATGAGGTCTGGCTGGTAGTCTCA
GGTATTGGAGTCAAAATTAA

Downstream 100 bases:

>100_bases
GCCGATTATTACGGACGAGACATCTTATGCAGACACTACACGCTTTACTCCGTGACATTCCTGCGCCGGACGCCGAGGCG
ATGGCGCGTGCGCAGCAACA

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSKLFWAMLAFISRLPVPSRWSQGLDFEQYSRGIVMFPFIGLILGGVSGLIFILLQPWCGIPLAALFCILALALLTGGFH
LDGLADTCDGIFSARRRERMLEIMRDSRLGTHGGLALIFVLLAKILVVSELALRGTPMLAALAAACAAGRGSAVLLMYRH
RYAREEGLGNVFIGKVSGRQTCITLGLAVIVATVLLPGMQGLAAMVVTCAAIFILGQLLKRTLGGQTGDTLGAAIELGEL
IFLLALL

Sequences:

>Translated_247_residues
MSKLFWAMLAFISRLPVPSRWSQGLDFEQYSRGIVMFPFIGLILGGVSGLIFILLQPWCGIPLAALFCILALALLTGGFH
LDGLADTCDGIFSARRRERMLEIMRDSRLGTHGGLALIFVLLAKILVVSELALRGTPMLAALAAACAAGRGSAVLLMYRH
RYAREEGLGNVFIGKVSGRQTCITLGLAVIVATVLLPGMQGLAAMVVTCAAIFILGQLLKRTLGGQTGDTLGAAIELGEL
IFLLALL
>Mature_246_residues
SKLFWAMLAFISRLPVPSRWSQGLDFEQYSRGIVMFPFIGLILGGVSGLIFILLQPWCGIPLAALFCILALALLTGGFHL
DGLADTCDGIFSARRRERMLEIMRDSRLGTHGGLALIFVLLAKILVVSELALRGTPMLAALAAACAAGRGSAVLLMYRHR
YAREEGLGNVFIGKVSGRQTCITLGLAVIVATVLLPGMQGLAAMVVTCAAIFILGQLLKRTLGGQTGDTLGAAIELGELI
FLLALL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=247, Percent_Identity=82.1862348178138, Blast_Score=412, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 26316; Mature: 26185

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLFWAMLAFISRLPVPSRWSQGLDFEQYSRGIVMFPFIGLILGGVSGLIFILLQPWCG
CHHHHHHHHHHHHHCCCCCHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
IPLAALFCILALALLTGGFHLDGLADTCDGIFSARRRERMLEIMRDSRLGTHGGLALIFV
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
LLAKILVVSELALRGTPMLAALAAACAAGRGSAVLLMYRHRYAREEGLGNVFIGKVSGRQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCEEEECCCCCH
TCITLGLAVIVATVLLPGMQGLAAMVVTCAAIFILGQLLKRTLGGQTGDTLGAAIELGEL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
IFLLALL
HHHHHHC
>Mature Secondary Structure 
SKLFWAMLAFISRLPVPSRWSQGLDFEQYSRGIVMFPFIGLILGGVSGLIFILLQPWCG
HHHHHHHHHHHHHCCCCCHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
IPLAALFCILALALLTGGFHLDGLADTCDGIFSARRRERMLEIMRDSRLGTHGGLALIFV
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
LLAKILVVSELALRGTPMLAALAAACAAGRGSAVLLMYRHRYAREEGLGNVFIGKVSGRQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCEEEECCCCCH
TCITLGLAVIVATVLLPGMQGLAAMVVTCAAIFILGQLLKRTLGGQTGDTLGAAIELGEL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
IFLLALL
HHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA