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The map label for this gene is fliF

Identifier: 16765307

GI number: 16765307

Start: 2056539

End: 2058221

Strand: Direct

Name: fliF

Synonym: STM1969

Alternate gene names: 16765307

Gene position: 2056539-2058221 (Clockwise)

Preceding gene: 16765304

Following gene: 16765308

Centisome position: 42.34

GC content: 57.16

Gene sequence:

>1683_bases
ATGAGTGCGACTGCATCGACTGCAACCCAACCTAAACCTCTCGAGTGGCTTAATCGCCTACGCGCGAATCCCCGAATTCC
GTTAATTGTGGCGGGTTCCGCTGCCGTGGCGATCGTCGTTGCGATGGTGCTGTGGGCCAAAACGCCCGATTACCGCACGC
TATTTAGCAACCTTTCCGATCAGGACGGCGGCGCGATCGTCGCGCAGTTGACCCAGATGAATATCCCCTATCGCTTTGCC
AATGGCAGCGGCGCGATTGAAGTGCCCGCCGACAAAGTGCATGAACTGCGTCTGCGTCTGGCGCAGCAGGGGCTGCCTAA
AGGCGGCGCGGTAGGCTTTGAACTGCTTGATCAGGAAAAATTCGGTATCAGCCAGTTCAGCGAGCAGGTGAATTATCAGC
GCGCGCTGGAAGGTGAACTGGCCCGGACGATCGAGACGCTGGGGCCGGTAAAAAGCGCCCGCGTTCACCTGGCGATGCCA
AAGCCGTCGCTATTTGTGCGGGAACAAAAATCCCCTTCCGCCTCCGTCACCGTCACACTGGAACCGGGACGGGCGCTGGA
TGAAGGACAAATTAGCGCCGTGGTACATCTGGTTTCCAGCGCTGTCGCCGGTTTGCCGCCAGGAAACGTCACTCTGGTCG
ATCAATCCGGTCATCTGCTCACGCAATCCAATACCAGCGGTCGCGACCTTAATGACGCGCAATTAAAATTTGCTAATGAT
GTGGAAAGCCGTATCCAACGCCGTATCGAAGCCATTCTGTCGCCTATCGTCGGGAACGGTAATGTTCACGCTCAGGTAAC
CGCCCAGTTGGATTTTGCCAATAAAGAGCAGACGGAAGAGCACTACAGCCCGAACGGCGATGCGTCGAAGGCCACTCTGC
GTTCACGTCAGTTGAATATTAGCGAACAGGTAGGCGCGGGCTATCCCGGCGGCGTGCCTGGCGCGCTGTCGAACCAACCA
GCGCCGCCTAACGAGGCGCCGATCGCCACGCCGCCGACCAATCAACAAAACGCGCAGAATACGCCACAAACCAGCACCAG
TACCAACAGTAACAGTGCTGGCCCGCGTAGTACACAGCGTAATGAAACCAGTAACTACGAAGTGGACCGCACCATTCGTC
ACACCAAAATGAATGTGGGCGATATTGAGCGTCTCTCCGTGGCCGTCGTGGTGAATTACAAAACGCTGGCGGATGGCAAA
CCGCTGCCATTGACCGCCGATCAGATGAAACAGATCGAGGATTTAACCCGTGAAGCGATGGGTTTTTCTGATAAACGCGG
CGATACGCTAAATGTGGTGAATTCACCGTTTAGCGCAGTGGATAACACCGGCGGCGAACTGCCCTTCTGGCAACAGCAGT
CCTTTATCGATCAGTTGCTCGCGGCAGGTCGCTGGTTGCTGGTGCTGGTGGTGGCCTGGATTCTGTGGCGCAAAGCGGTA
CGTCCGCAGTTGACGCGCCGCGTTGAAGAAGCGAAAGCGGCGCAGGAACAGGCGCAGGTTCGCCAGGAAACCGAAGAAGC
CGTGGAAGTACGCCTTAGCAAAGACGAGCAGCTCCAGCAACGCCGGGCTAACCAGCGTCTGGGCGCTGAGGTGATGAGCC
AGCGTATTCGCGAAATGTCAGACAACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATGAGTAACGATCATGAG
TAA

Upstream 100 bases:

>100_bases
CAAATAATGAGACCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTGTTCCACTTTGCAAAAAACGCCGTCCAC
GATCAGTCACGAGGTGCGAG

Downstream 100 bases:

>100_bases
TCTTAGCGGTACCGATAAAAGCGTCATTTTGTTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTTAAACACCTCT
CCACCCGCGAAGTACAGGCG

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MSATASTATQPKPLEWLNRLRANPRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSDQDGGAIVAQLTQMNIPYRFA
NGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELARTIETLGPVKSARVHLAMP
KPSLFVREQKSPSASVTVTLEPGRALDEGQISAVVHLVSSAVAGLPPGNVTLVDQSGHLLTQSNTSGRDLNDAQLKFAND
VESRIQRRIEAILSPIVGNGNVHAQVTAQLDFANKEQTEEHYSPNGDASKATLRSRQLNISEQVGAGYPGGVPGALSNQP
APPNEAPIATPPTNQQNAQNTPQTSTSTNSNSAGPRSTQRNETSNYEVDRTIRHTKMNVGDIERLSVAVVVNYKTLADGK
PLPLTADQMKQIEDLTREAMGFSDKRGDTLNVVNSPFSAVDNTGGELPFWQQQSFIDQLLAAGRWLLVLVVAWILWRKAV
RPQLTRRVEEAKAAQEQAQVRQETEEAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWMSNDHE

Sequences:

>Translated_560_residues
MSATASTATQPKPLEWLNRLRANPRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSDQDGGAIVAQLTQMNIPYRFA
NGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELARTIETLGPVKSARVHLAMP
KPSLFVREQKSPSASVTVTLEPGRALDEGQISAVVHLVSSAVAGLPPGNVTLVDQSGHLLTQSNTSGRDLNDAQLKFAND
VESRIQRRIEAILSPIVGNGNVHAQVTAQLDFANKEQTEEHYSPNGDASKATLRSRQLNISEQVGAGYPGGVPGALSNQP
APPNEAPIATPPTNQQNAQNTPQTSTSTNSNSAGPRSTQRNETSNYEVDRTIRHTKMNVGDIERLSVAVVVNYKTLADGK
PLPLTADQMKQIEDLTREAMGFSDKRGDTLNVVNSPFSAVDNTGGELPFWQQQSFIDQLLAAGRWLLVLVVAWILWRKAV
RPQLTRRVEEAKAAQEQAQVRQETEEAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWMSNDHE
>Mature_559_residues
SATASTATQPKPLEWLNRLRANPRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSDQDGGAIVAQLTQMNIPYRFAN
GSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELARTIETLGPVKSARVHLAMPK
PSLFVREQKSPSASVTVTLEPGRALDEGQISAVVHLVSSAVAGLPPGNVTLVDQSGHLLTQSNTSGRDLNDAQLKFANDV
ESRIQRRIEAILSPIVGNGNVHAQVTAQLDFANKEQTEEHYSPNGDASKATLRSRQLNISEQVGAGYPGGVPGALSNQPA
PPNEAPIATPPTNQQNAQNTPQTSTSTNSNSAGPRSTQRNETSNYEVDRTIRHTKMNVGDIERLSVAVVVNYKTLADGKP
LPLTADQMKQIEDLTREAMGFSDKRGDTLNVVNSPFSAVDNTGGELPFWQQQSFIDQLLAAGRWLLVLVVAWILWRKAVR
PQLTRRVEEAKAAQEQAQVRQETEEAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWMSNDHE

Specific function: The M ring may be actively involved in energy transduction

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family

Homologues:

Organism=Escherichia coli, GI1788248, Length=555, Percent_Identity=86.3063063063063, Blast_Score=957, Evalue=0.0,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): FLIF_SALTY (P15928)

Other databases:

- EMBL:   M24462
- EMBL:   AE006468
- EMBL:   M84993
- PIR:   C32887
- RefSeq:   NP_460922.1
- ProteinModelPortal:   P15928
- IntAct:   P15928
- PRIDE:   P15928
- GeneID:   1253490
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM1969
- NMPDR:   fig|99287.1.peg.1902
- HOGENOM:   HBG661951
- OMA:   FMEATRY
- ProtClustDB:   PRK06007
- BioCyc:   STYP99287:STM1969-MONOMER
- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182
- PRINTS:   PR01009
- TIGRFAMs:   TIGR00206

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C

EC number: NA

Molecular weight: Translated: 61231; Mature: 61099

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xefe5c0c)-; HASH(0xd116ce4)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATASTATQPKPLEWLNRLRANPRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSD
CCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHCCCCCHHHHHHCCCC
QDGGAIVAQLTQMNIPYRFANGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEK
CCCCCEEHHHHHCCCCEEECCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHH
FGISQFSEQVNYQRALEGELARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCCCEEEEEE
EPGRALDEGQISAVVHLVSSAVAGLPPGNVTLVDQSGHLLTQSNTSGRDLNDAQLKFAND
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHH
VESRIQRRIEAILSPIVGNGNVHAQVTAQLDFANKEQTEEHYSPNGDASKATLRSRQLNI
HHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHCCC
SEQVGAGYPGGVPGALSNQPAPPNEAPIATPPTNQQNAQNTPQTSTSTNSNSAGPRSTQR
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
NETSNYEVDRTIRHTKMNVGDIERLSVAVVVNYKTLADGKPLPLTADQMKQIEDLTREAM
CCCCCCCHHHHHHHHCCCCCCCCEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHC
GFSDKRGDTLNVVNSPFSAVDNTGGELPFWQQQSFIDQLLAAGRWLLVLVVAWILWRKAV
CCCCCCCCEEEECCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
RPQLTRRVEEAKAAQEQAQVRQETEEAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
DNDPRVVALVIRQWMSNDHE
CCCCHHHHHHHHHHHCCCCH
>Mature Secondary Structure 
SATASTATQPKPLEWLNRLRANPRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSD
CCCCCCCCCCCHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHCCCCCHHHHHHCCCC
QDGGAIVAQLTQMNIPYRFANGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEK
CCCCCEEHHHHHCCCCEEECCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHH
FGISQFSEQVNYQRALEGELARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCCCEEEEEE
EPGRALDEGQISAVVHLVSSAVAGLPPGNVTLVDQSGHLLTQSNTSGRDLNDAQLKFAND
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHH
VESRIQRRIEAILSPIVGNGNVHAQVTAQLDFANKEQTEEHYSPNGDASKATLRSRQLNI
HHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHCCC
SEQVGAGYPGGVPGALSNQPAPPNEAPIATPPTNQQNAQNTPQTSTSTNSNSAGPRSTQR
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
NETSNYEVDRTIRHTKMNVGDIERLSVAVVVNYKTLADGKPLPLTADQMKQIEDLTREAM
CCCCCCCHHHHHHHHCCCCCCCCEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHC
GFSDKRGDTLNVVNSPFSAVDNTGGELPFWQQQSFIDQLLAAGRWLLVLVVAWILWRKAV
CCCCCCCCEEEECCCCHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
RPQLTRRVEEAKAAQEQAQVRQETEEAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
DNDPRVVALVIRQWMSNDHE
CCCCHHHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2544561; 11677609; 1551848