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The map label for this gene is amyA

Identifier: 16765301

GI number: 16765301

Start: 2051667

End: 2053151

Strand: Direct

Name: amyA

Synonym: STM1963

Alternate gene names: 16765301

Gene position: 2051667-2053151 (Clockwise)

Preceding gene: 16765300

Following gene: 308065962

Centisome position: 42.24

GC content: 51.38

Gene sequence:

>1485_bases
ATGAAAAACCCCACGTTATTGCAGTACTTCCACTGGTATTATCCCGACGGCGGTAAACTCTGGTCTGAGCTGGCGGAACG
TGCTGATGGGCTGAATGATATCGGTATCAATATGGTCTGGCTACCGCCCGCCTGTAAAGGCGCCTCCGGCGGCTATTCCG
TAGGCTATGATACCTACGACCTGTTTGACCTCGGCGAATTTGACCAAAAAGGAACTATCGCGACAAAGTACGGCGATAAA
CGCCAGTTACTGACGGCGATAGACGCGCTCAAAAAAAATAATATTGCGGTGCTGCTCGACGTCGTCGTGAACCACAAAAT
GGGCGCAGACGAAAAAGAACGTATTCGCGTTCAGCGCGTGAATCAGGATGACCGCACGCAAATCGATGACAACATCATTG
AATGCGAAGGCTGGACGCGCTACACCTTCCCTGCCCGCGCGGGCCAGTATTCCAACTTTATTTGGGACTATCACTGTTTC
AGCGGCATTGATCACATCGAGAATCCCGACGAAGACGGCATTTTTAAGATCGTCAATGACTATACCGGCGATGGCTGGAA
CGATCAGGTTGATGATGAGATGGGTAATTTCGACTATCTGATGGGTGAAAATATCGATTTTCGCAATCATGCGGTTACGG
AAGAGATTAAATATTGGGCTCGTTGGGTCATGGAACAAACCCACTGTGACGGCTTTCGCCTGGACGCGGTAAAACATATA
CCCGCCTGGTTTTATAAAGAATGGATTGAGCATGTACAGGCGGTTGCGCCAAAACCGCTGTTTATTGTCGCAGAATACTG
GTCGCATGAAGTGGATAAACTGCAAACGTACATCGATCAGGTCGACGGGAAAACCATGCTGTTCGACGCGCCGTTGCAGA
TGAAATTTCACGAGGCCTCGCGCCAGGGCGCGGAGTATGACATGCGCCACATTTTCACCGGCACGCTGGTAGAAGCCGAC
CCTTTTCATGCGGTGACGCTGGTCGCTAACCACGATACACAACCGTTACAGGCGCTGGAAGCGCCGGTAGAACCCTGGTT
CAAACCATTGGCCTATGCGCTGATCCTGCTTCGTGAAAACGGCGTGCCGTCAGTGTTTTATCCCGATTTATACGGCGCCA
GCTATGAGGATAGCGGCGAAAATGGCGAGACCTGTCGGGTCGACATGCCGGTGATTAACCAACTGGATCGGCTGATCCTC
GCTCGTCAGCGTTTTGCGCACGGTATACAAACACTCTTTTTCGATCATCCTAACTGTATCGCCTTTAGTCGCAGCGGTAC
TGAAGAGAATCCAGGCTGTGTGGTCGTACTTTCCAATGGCGACGACGGTGAAAAAACCCTCCTGCTCGGCGACAATTACG
CTAACAAGACCTGGCGTGATTTTCTGGGAAACCGGGATGAGTATGTTGTAACTAATGATCAAGGCGAAGCGACGTTCTTC
TGCAACGCAGGCAGCGTCAGCGTGTGGGTCATTGAGGACGTGTGA

Upstream 100 bases:

>100_bases
TCGTGCCAGATGCGCCTGGCGCCTCATAATATTTTCCCGTCTCGTATGAAAATTCTTCCATACTCCAGAGGTCGGCTAAA
CGACTTCTGGAGCACGGAAG

Downstream 100 bases:

>100_bases
TGAGCGTTCCCGGCGGCAGAGCGCTGCCGGGAAGCGAAATTACGGCAGCGGCGTCGGCAGCGGCGTCGGCAGCGGCGTTT
TTTCTAACGCGGCGGCGCAC

Product: cytoplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MKNPTLLQYFHWYYPDGGKLWSELAERADGLNDIGINMVWLPPACKGASGGYSVGYDTYDLFDLGEFDQKGTIATKYGDK
RQLLTAIDALKKNNIAVLLDVVVNHKMGADEKERIRVQRVNQDDRTQIDDNIIECEGWTRYTFPARAGQYSNFIWDYHCF
SGIDHIENPDEDGIFKIVNDYTGDGWNDQVDDEMGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTHCDGFRLDAVKHI
PAWFYKEWIEHVQAVAPKPLFIVAEYWSHEVDKLQTYIDQVDGKTMLFDAPLQMKFHEASRQGAEYDMRHIFTGTLVEAD
PFHAVTLVANHDTQPLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGASYEDSGENGETCRVDMPVINQLDRLIL
ARQRFAHGIQTLFFDHPNCIAFSRSGTEENPGCVVVLSNGDDGEKTLLLGDNYANKTWRDFLGNRDEYVVTNDQGEATFF
CNAGSVSVWVIEDV

Sequences:

>Translated_494_residues
MKNPTLLQYFHWYYPDGGKLWSELAERADGLNDIGINMVWLPPACKGASGGYSVGYDTYDLFDLGEFDQKGTIATKYGDK
RQLLTAIDALKKNNIAVLLDVVVNHKMGADEKERIRVQRVNQDDRTQIDDNIIECEGWTRYTFPARAGQYSNFIWDYHCF
SGIDHIENPDEDGIFKIVNDYTGDGWNDQVDDEMGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTHCDGFRLDAVKHI
PAWFYKEWIEHVQAVAPKPLFIVAEYWSHEVDKLQTYIDQVDGKTMLFDAPLQMKFHEASRQGAEYDMRHIFTGTLVEAD
PFHAVTLVANHDTQPLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGASYEDSGENGETCRVDMPVINQLDRLIL
ARQRFAHGIQTLFFDHPNCIAFSRSGTEENPGCVVVLSNGDDGEKTLLLGDNYANKTWRDFLGNRDEYVVTNDQGEATFF
CNAGSVSVWVIEDV
>Mature_494_residues
MKNPTLLQYFHWYYPDGGKLWSELAERADGLNDIGINMVWLPPACKGASGGYSVGYDTYDLFDLGEFDQKGTIATKYGDK
RQLLTAIDALKKNNIAVLLDVVVNHKMGADEKERIRVQRVNQDDRTQIDDNIIECEGWTRYTFPARAGQYSNFIWDYHCF
SGIDHIENPDEDGIFKIVNDYTGDGWNDQVDDEMGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTHCDGFRLDAVKHI
PAWFYKEWIEHVQAVAPKPLFIVAEYWSHEVDKLQTYIDQVDGKTMLFDAPLQMKFHEASRQGAEYDMRHIFTGTLVEAD
PFHAVTLVANHDTQPLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGASYEDSGENGETCRVDMPVINQLDRLIL
ARQRFAHGIQTLFFDHPNCIAFSRSGTEENPGCVVVLSNGDDGEKTLLLGDNYANKTWRDFLGNRDEYVVTNDQGEATFF
CNAGSVSVWVIEDV

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Escherichia coli, GI1788236, Length=494, Percent_Identity=88.663967611336, Blast_Score=921, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMY2_SALTY (P26613)

Other databases:

- EMBL:   L01643
- EMBL:   AE006468
- EMBL:   M85241
- EMBL:   L13280
- PIR:   B45738
- RefSeq:   NP_460916.1
- ProteinModelPortal:   P26613
- GeneID:   1253484
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM1963
- NMPDR:   fig|99287.1.peg.1897
- HOGENOM:   HBG284842
- OMA:   VWIPPAY
- ProtClustDB:   PRK09441
- BioCyc:   STYP99287:STM1963-MONOMER
- BRENDA:   3.2.1.1
- GO:   GO:0005737
- InterPro:   IPR013776
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- PIRSF:   PIRSF001021
- SMART:   SM00642

Pfam domain/function: PF00128 Alpha-amylase; SSF51445 Glyco_hydro_cat

EC number: =3.2.1.1

Molecular weight: Translated: 56523; Mature: 56523

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: NA

Important sites: ACT_SITE 235-235 ACT_SITE 265-265

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNPTLLQYFHWYYPDGGKLWSELAERADGLNDIGINMVWLPPACKGASGGYSVGYDTYD
CCCCCEEEEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEE
LFDLGEFDQKGTIATKYGDKRQLLTAIDALKKNNIAVLLDVVVNHKMGADEKERIRVQRV
ECCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEEEEHHCCCCCCCHHHCEEEEEC
NQDDRTQIDDNIIECEGWTRYTFPARAGQYSNFIWDYHCFSGIDHIENPDEDGIFKIVND
CCCCCCCCCCCEEEECCCEEEECCCCCCCCCCEEEEEHHHCCCCCCCCCCCCCEEEEEEC
YTGDGWNDQVDDEMGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTHCDGFRLDAVKHI
CCCCCCCCCCHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
PAWFYKEWIEHVQAVAPKPLFIVAEYWSHEVDKLQTYIDQVDGKTMLFDAPLQMKFHEAS
HHHHHHHHHHHHHHHCCCCEEEEEHHHCCHHHHHHHHHHHCCCCEEEEECCCCEEEHHHH
RQGAEYDMRHIFTGTLVEADPFHAVTLVANHDTQPLQALEAPVEPWFKPLAYALILLREN
CCCCCCCHHHHEECEEEECCCCEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHEEEEECC
GVPSVFYPDLYGASYEDSGENGETCRVDMPVINQLDRLILARQRFAHGIQTLFFDHPNCI
CCCCEECCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHEECCCCEE
AFSRSGTEENPGCVVVLSNGDDGEKTLLLGDNYANKTWRDFLGNRDEYVVTNDQGEATFF
EEECCCCCCCCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHCCCCCEEEECCCCCEEEE
CNAGSVSVWVIEDV
EECCCEEEEEEECC
>Mature Secondary Structure
MKNPTLLQYFHWYYPDGGKLWSELAERADGLNDIGINMVWLPPACKGASGGYSVGYDTYD
CCCCCEEEEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEE
LFDLGEFDQKGTIATKYGDKRQLLTAIDALKKNNIAVLLDVVVNHKMGADEKERIRVQRV
ECCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEEEEHHCCCCCCCHHHCEEEEEC
NQDDRTQIDDNIIECEGWTRYTFPARAGQYSNFIWDYHCFSGIDHIENPDEDGIFKIVND
CCCCCCCCCCCEEEECCCEEEECCCCCCCCCCEEEEEHHHCCCCCCCCCCCCCEEEEEEC
YTGDGWNDQVDDEMGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTHCDGFRLDAVKHI
CCCCCCCCCCHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
PAWFYKEWIEHVQAVAPKPLFIVAEYWSHEVDKLQTYIDQVDGKTMLFDAPLQMKFHEAS
HHHHHHHHHHHHHHHCCCCEEEEEHHHCCHHHHHHHHHHHCCCCEEEEECCCCEEEHHHH
RQGAEYDMRHIFTGTLVEADPFHAVTLVANHDTQPLQALEAPVEPWFKPLAYALILLREN
CCCCCCCHHHHEECEEEECCCCEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHEEEEECC
GVPSVFYPDLYGASYEDSGENGETCRVDMPVINQLDRLILARQRFAHGIQTLFFDHPNCI
CCCCEECCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHEECCCCEE
AFSRSGTEENPGCVVVLSNGDDGEKTLLLGDNYANKTWRDFLGNRDEYVVTNDQGEATFF
EEECCCCCCCCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHCCCCCEEEECCCCCEEEE
CNAGSVSVWVIEDV
EECCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400215; 11677609; 1527488; 8371104