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The map label for this gene is mrdA

Identifier: 16765252

GI number: 16765252

Start: 2004519

End: 2006390

Strand: Direct

Name: mrdA

Synonym: STM1910

Alternate gene names: 16765252

Gene position: 2004519-2006390 (Clockwise)

Preceding gene: 16765251

Following gene: 16765269

Centisome position: 41.27

GC content: 51.92

Gene sequence:

>1872_bases
ATGACTTTTAAAGACTTTGATGCGGAAGAGAAACTCTTCCTGAGACGTGTGATTGTGGCGTTTGGTGTAGTGGTTGTCTG
CTTTGGTATCCTGATCTTCAACCTCTATAACTTGCAAATCCGCCAGCACCACTACTACACCACCCGTTCGAATGAGAACG
ATATCAAGATGCTACCCGTCGCGCCCACGCGCGGCATCATTTACGATCGCAACGGCATTCCGCTGGTGCGTAACGTAACC
TGGTATGATATCGCGGTTACGCCCTATAAAATTGCCGATATGGATGCGCTGCTAAAACAGCTCACCCCTATTGTTGATCT
CTCCCCCGACGATATCTCCGACTTTCGCCGTGCGCTAAAATCCAGTAGCCGCTATCGTCCGGTGGTGCTCAAAAACGCGC
TAACGGATGTTGAAATCGCACGCTTTGCGGTCAATCAGTTTCATTTCAACGGCGTCACGATCAATAGCTATCAGGATCGG
CAGTATCCTTACGGCGCGGAGCTGGCGCATGTTCTCGGTTACGTGTCAAAGATAAACGATAACGACCTTAAAGCGCTGGA
TAAAAAAGGGCTGGCAGAGAATTACGCGGCGGACCATAACATCGGTAAACAGGGGATTGAGCGTTACTACGAAAACGATC
TTCATGGTAAAACCGGTTATCAGGAGGTCGAGGTGGATAATCACGGGCGTATTGTCCGCCTGCTGAAAGACGTTCCTCCC
ATCGCGGGTAAGAACATTCACCTGACGCTGGATCTCCATTTACAAGAGTACATTGAAAGTTTGCTGGCCGGGCAGCGTGC
TGCCGTACTGGTTGAAGATCCGCACGATGGTTCAGTGCTGGCGATGGTATCTATGCCCAGCTACGACCCGAACCCCTTTG
TAAAAGGCATTAGCTATCAGGATTACGGCAAACTGCTGCACGATAAAAATCTGCCGCTGATCAACCGCGTGACGCAAGGG
CTTTATCCTCCGGCGTCAACGGTTAAACCCTATATGGCAATGTCCGCGCTGTTATGCGGGATTATTACGCCGCAAACCAC
CTTCTTTGGCGCGCCAACCTGGACGCTTCCCGGCACGCAGCGGCATTACCGGGACTGGAAAAAAACCGGACACGGTATGC
TGGATGTCACTAAAGCGATTGAGGAATCTGCGGATACCTTTTTCTATCAGGTCGCTTATATGATGGGTATTGACCGTATC
GACACGATGTTGTCTCAGTTCGGCTATGGCAAACCGACGGGCATCGACCTTAATGAAGAATATGACGGGCTGCTTCCCAG
CCGCGCATGGAAACAGCGGGTCCATAAAAAAGCCTGGTATCAGGGCGATACCATTTCTGTCGGCATCGGCCAGGGTTACT
GGATTGCCACCCCCATCCAGATGGTAAAAGCGATGGTGGCGCTCATCAACAACGGTAAGGTGATTGCCCCTCACCTGTTA
CTCAATGAAGAGAGCGGCAAAACGGTGGTTCCGTATCGTCCTTCAGGAACACCGGCACAGATAGCCGATCCGGCGTCGCC
ATACTGGGGACTGGTGCGTCAGGCGATGTATGGCATGGCGAATGCACCCAACGGAACGGGCTATAAGTTCTTTCACACCG
CGCCCTACGGCATTGCGGCCAAAAGCGGCACGTCGCAGGTATTTAGCCTGAAAGAAAATCAGACCTACAACGCGAAAATG
ATCCCCATTCGCCTGCGCGATCATGTGTTTTATACCGCTTTTGCACCGTATAAAAACCCTAAGGTCGCTATTGCCTTGAT
TCTGGAAAACGGCGGAAGCGACGGCGTTACCGCCGCACCCATCATGCGAAAAATCCTTGACCACCTGTTTGATCCACAGG
CTGATACCACACAGCCGGATCAGGCGCCATAG

Upstream 100 bases:

>100_bases
CAAGGAATGACACATTTCTATAGAGAGATGGAACATCTTTTACCTGTGCTGGCGCTGACCGCCCCGTATCATTAGGCGGC
GGATTGTTCAGGTACAGGTA

Downstream 100 bases:

>100_bases
TCAAATAAGCAAAGCGCCATCAGACAAGGATACGGCCGGAATATCTGCATACATCAGAAATAGTTACGCAGATATTCCGT
CAAACACAGCATTGCCATCG

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2

Number of amino acids: Translated: 623; Mature: 622

Protein sequence:

>623_residues
MTFKDFDAEEKLFLRRVIVAFGVVVVCFGILIFNLYNLQIRQHHYYTTRSNENDIKMLPVAPTRGIIYDRNGIPLVRNVT
WYDIAVTPYKIADMDALLKQLTPIVDLSPDDISDFRRALKSSSRYRPVVLKNALTDVEIARFAVNQFHFNGVTINSYQDR
QYPYGAELAHVLGYVSKINDNDLKALDKKGLAENYAADHNIGKQGIERYYENDLHGKTGYQEVEVDNHGRIVRLLKDVPP
IAGKNIHLTLDLHLQEYIESLLAGQRAAVLVEDPHDGSVLAMVSMPSYDPNPFVKGISYQDYGKLLHDKNLPLINRVTQG
LYPPASTVKPYMAMSALLCGIITPQTTFFGAPTWTLPGTQRHYRDWKKTGHGMLDVTKAIEESADTFFYQVAYMMGIDRI
DTMLSQFGYGKPTGIDLNEEYDGLLPSRAWKQRVHKKAWYQGDTISVGIGQGYWIATPIQMVKAMVALINNGKVIAPHLL
LNEESGKTVVPYRPSGTPAQIADPASPYWGLVRQAMYGMANAPNGTGYKFFHTAPYGIAAKSGTSQVFSLKENQTYNAKM
IPIRLRDHVFYTAFAPYKNPKVAIALILENGGSDGVTAAPIMRKILDHLFDPQADTTQPDQAP

Sequences:

>Translated_623_residues
MTFKDFDAEEKLFLRRVIVAFGVVVVCFGILIFNLYNLQIRQHHYYTTRSNENDIKMLPVAPTRGIIYDRNGIPLVRNVT
WYDIAVTPYKIADMDALLKQLTPIVDLSPDDISDFRRALKSSSRYRPVVLKNALTDVEIARFAVNQFHFNGVTINSYQDR
QYPYGAELAHVLGYVSKINDNDLKALDKKGLAENYAADHNIGKQGIERYYENDLHGKTGYQEVEVDNHGRIVRLLKDVPP
IAGKNIHLTLDLHLQEYIESLLAGQRAAVLVEDPHDGSVLAMVSMPSYDPNPFVKGISYQDYGKLLHDKNLPLINRVTQG
LYPPASTVKPYMAMSALLCGIITPQTTFFGAPTWTLPGTQRHYRDWKKTGHGMLDVTKAIEESADTFFYQVAYMMGIDRI
DTMLSQFGYGKPTGIDLNEEYDGLLPSRAWKQRVHKKAWYQGDTISVGIGQGYWIATPIQMVKAMVALINNGKVIAPHLL
LNEESGKTVVPYRPSGTPAQIADPASPYWGLVRQAMYGMANAPNGTGYKFFHTAPYGIAAKSGTSQVFSLKENQTYNAKM
IPIRLRDHVFYTAFAPYKNPKVAIALILENGGSDGVTAAPIMRKILDHLFDPQADTTQPDQAP
>Mature_622_residues
TFKDFDAEEKLFLRRVIVAFGVVVVCFGILIFNLYNLQIRQHHYYTTRSNENDIKMLPVAPTRGIIYDRNGIPLVRNVTW
YDIAVTPYKIADMDALLKQLTPIVDLSPDDISDFRRALKSSSRYRPVVLKNALTDVEIARFAVNQFHFNGVTINSYQDRQ
YPYGAELAHVLGYVSKINDNDLKALDKKGLAENYAADHNIGKQGIERYYENDLHGKTGYQEVEVDNHGRIVRLLKDVPPI
AGKNIHLTLDLHLQEYIESLLAGQRAAVLVEDPHDGSVLAMVSMPSYDPNPFVKGISYQDYGKLLHDKNLPLINRVTQGL
YPPASTVKPYMAMSALLCGIITPQTTFFGAPTWTLPGTQRHYRDWKKTGHGMLDVTKAIEESADTFFYQVAYMMGIDRID
TMLSQFGYGKPTGIDLNEEYDGLLPSRAWKQRVHKKAWYQGDTISVGIGQGYWIATPIQMVKAMVALINNGKVIAPHLLL
NEESGKTVVPYRPSGTPAQIADPASPYWGLVRQAMYGMANAPNGTGYKFFHTAPYGIAAKSGTSQVFSLKENQTYNAKMI
PIRLRDHVFYTAFAPYKNPKVAIALILENGGSDGVTAAPIMRKILDHLFDPQADTTQPDQAP

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family

Homologues:

Organism=Escherichia coli, GI1786854, Length=623, Percent_Identity=58.5874799357945, Blast_Score=739, Evalue=0.0,
Organism=Escherichia coli, GI1786272, Length=635, Percent_Identity=22.5196850393701, Blast_Score=113, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PBP2_SALTY (P74872)

Other databases:

- EMBL:   AE006468
- EMBL:   U62714
- RefSeq:   NP_460867.1
- ProteinModelPortal:   P74872
- SMR:   P74872
- GeneID:   1253431
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM1910
- NMPDR:   fig|99287.1.peg.1850
- HOGENOM:   HBG712588
- OMA:   MYLNIDA
- ProtClustDB:   CLSK894745
- BioCyc:   STYP99287:STM1910-MONOMER
- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790
- TIGRFAMs:   TIGR03423

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase; SSF56519 PBP_dimer; SSF56601 PBP_transp_fold

EC number: NA

Molecular weight: Translated: 69834; Mature: 69703

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: NA

Important sites: ACT_SITE 326-326

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFKDFDAEEKLFLRRVIVAFGVVVVCFGILIFNLYNLQIRQHHYYTTRSNENDIKMLPV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCEEEEEC
APTRGIIYDRNGIPLVRNVTWYDIAVTPYKIADMDALLKQLTPIVDLSPDDISDFRRALK
CCCCCEEECCCCCCEEECCEEEEEEECCEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
SSSRYRPVVLKNALTDVEIARFAVNQFHFNGVTINSYQDRQYPYGAELAHVLGYVSKIND
CCCCCCCCHHHHHHHHHHHHHHHHHHEEECCEEECCCCCCCCCCHHHHHHHHHHHHHCCC
NDLKALDKKGLAENYAADHNIGKQGIERYYENDLHGKTGYQEVEVDNHGRIVRLLKDVPP
CHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHCCC
IAGKNIHLTLDLHLQEYIESLLAGQRAAVLVEDPHDGSVLAMVSMPSYDPNPFVKGISYQ
CCCCEEEEEEEHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHCCCCHH
DYGKLLHDKNLPLINRVTQGLYPPASTVKPYMAMSALLCGIITPQTTFFGAPTWTLPGTQ
HHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCH
RHYRDWKKTGHGMLDVTKAIEESADTFFYQVAYMMGIDRIDTMLSQFGYGKPTGIDLNEE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
YDGLLPSRAWKQRVHKKAWYQGDTISVGIGQGYWIATPIQMVKAMVALINNGKVIAPHLL
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEECHHHHHHHHHHHHCCCCEEEEEEE
LNEESGKTVVPYRPSGTPAQIADPASPYWGLVRQAMYGMANAPNGTGYKFFHTAPYGIAA
EECCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEE
KSGTSQVFSLKENQTYNAKMIPIRLRDHVFYTAFAPYKNPKVAIALILENGGSDGVTAAP
CCCCCCEEEECCCCCCCEEEEEEEEECCEEEEEECCCCCCCEEEEEEEECCCCCCCCHHH
IMRKILDHLFDPQADTTQPDQAP
HHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TFKDFDAEEKLFLRRVIVAFGVVVVCFGILIFNLYNLQIRQHHYYTTRSNENDIKMLPV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCEEEEEC
APTRGIIYDRNGIPLVRNVTWYDIAVTPYKIADMDALLKQLTPIVDLSPDDISDFRRALK
CCCCCEEECCCCCCEEECCEEEEEEECCEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
SSSRYRPVVLKNALTDVEIARFAVNQFHFNGVTINSYQDRQYPYGAELAHVLGYVSKIND
CCCCCCCCHHHHHHHHHHHHHHHHHHEEECCEEECCCCCCCCCCHHHHHHHHHHHHHCCC
NDLKALDKKGLAENYAADHNIGKQGIERYYENDLHGKTGYQEVEVDNHGRIVRLLKDVPP
CHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHCCC
IAGKNIHLTLDLHLQEYIESLLAGQRAAVLVEDPHDGSVLAMVSMPSYDPNPFVKGISYQ
CCCCEEEEEEEHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHCCCCHH
DYGKLLHDKNLPLINRVTQGLYPPASTVKPYMAMSALLCGIITPQTTFFGAPTWTLPGTQ
HHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCH
RHYRDWKKTGHGMLDVTKAIEESADTFFYQVAYMMGIDRIDTMLSQFGYGKPTGIDLNEE
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
YDGLLPSRAWKQRVHKKAWYQGDTISVGIGQGYWIATPIQMVKAMVALINNGKVIAPHLL
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEECHHHHHHHHHHHHCCCCEEEEEEE
LNEESGKTVVPYRPSGTPAQIADPASPYWGLVRQAMYGMANAPNGTGYKFFHTAPYGIAA
EECCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEE
KSGTSQVFSLKENQTYNAKMIPIRLRDHVFYTAFAPYKNPKVAIALILENGGSDGVTAAP
CCCCCCEEEECCCCCCCEEEEEEEEECCEEEEEECCCCCCCEEEEEEEECCCCCCCCHHH
IMRKILDHLFDPQADTTQPDQAP
HHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677609; 8930920