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The map label for this gene is sopE2 [H]

Identifier: 16765196

GI number: 16765196

Start: 1952012

End: 1952734

Strand: Reverse

Name: sopE2 [H]

Synonym: STM1855

Alternate gene names: 16765196

Gene position: 1952734-1952012 (Counterclockwise)

Preceding gene: 16765198

Following gene: 16765195

Centisome position: 40.2

GC content: 41.77

Gene sequence:

>723_bases
GTGACTAACATAACACTATCCACCCAGCACTACAGAATCCATAGAAGTGACGTTGAACCAGTAAAAGAAAAAACAACGGA
GAAGGACATTTTTGCAAAAAGTATTACTGCCGTTAGAAATAGCTTTATCAGCCTGTCGACGAGTCTGTCAGATCGTTTTA
GCCTGCATCAACAAACAGACATACCGACTACCCATTTTCATCGTGGGAACGCTTCTGAGGGTAGGGCGGTATTAACCAGT
AAAACTGTTAAAGATTTTATGCTGCAAAAGCTCAATAGTCTGGATATCAAAGGTAATGCGAGTAAAGATCCGGCCTATGC
TCGTCAGACATGCGAAGCCATATTATCAGCCGTGTACAGTAATAATAAAGATCAATGTTGTAAATTACTCATCAGTAAAG
GGGTCAGTATTACCCCCTTTTTGAAAGAGATAGGAGAGGCTGCGCAGAATGCGGGGCTACCTGGGGAGATAAAAAATGGC
GTATTTACTCCAGGTGGGGCAGGGGCGAATCCTTTTGTCGTCCCCCTCATTGCTTCCGCGAGTATTAAATATCCGCATAT
GTTTATAAATCATAATCAGCAGGTATCTTTTAAAGCGTATGCTGAGAAAATCGTTATGAAAGAGGTTACGCCGCTGTTTA
ATAAGGGGACGATGCCAACGCCACAACAATTTCAGTTAACTATAGAAAATATTGCGAATAAGTATCTTCAGAATGCCTCC
TGA

Upstream 100 bases:

>100_bases
TCAATGAGTAAAAATGGTTGTGGAGAAGGTGGCTATTTTTTGAAAGCAAGAAATATAAACAAAGTGTAGCTATGCATAGT
TATCTAAAAGGAGAACTACC

Downstream 100 bases:

>100_bases
TGGTAGTAAATACAATACTGCTATTAACCATATGAATTAATAGCAGATTTTCTGAAACTATATATTCGTTTTTATGGCGA
CGCTTTTAACAAAACGTCGT

Product: type III-secreted effector protein

Products: NA

Alternate protein names: Effector protein sopE2; Toxin sopE2 [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MTNITLSTQHYRIHRSDVEPVKEKTTEKDIFAKSITAVRNSFISLSTSLSDRFSLHQQTDIPTTHFHRGNASEGRAVLTS
KTVKDFMLQKLNSLDIKGNASKDPAYARQTCEAILSAVYSNNKDQCCKLLISKGVSITPFLKEIGEAAQNAGLPGEIKNG
VFTPGGAGANPFVVPLIASASIKYPHMFINHNQQVSFKAYAEKIVMKEVTPLFNKGTMPTPQQFQLTIENIANKYLQNAS

Sequences:

>Translated_240_residues
MTNITLSTQHYRIHRSDVEPVKEKTTEKDIFAKSITAVRNSFISLSTSLSDRFSLHQQTDIPTTHFHRGNASEGRAVLTS
KTVKDFMLQKLNSLDIKGNASKDPAYARQTCEAILSAVYSNNKDQCCKLLISKGVSITPFLKEIGEAAQNAGLPGEIKNG
VFTPGGAGANPFVVPLIASASIKYPHMFINHNQQVSFKAYAEKIVMKEVTPLFNKGTMPTPQQFQLTIENIANKYLQNAS
>Mature_239_residues
TNITLSTQHYRIHRSDVEPVKEKTTEKDIFAKSITAVRNSFISLSTSLSDRFSLHQQTDIPTTHFHRGNASEGRAVLTSK
TVKDFMLQKLNSLDIKGNASKDPAYARQTCEAILSAVYSNNKDQCCKLLISKGVSITPFLKEIGEAAQNAGLPGEIKNGV
FTPGGAGANPFVVPLIASASIKYPHMFINHNQQVSFKAYAEKIVMKEVTPLFNKGTMPTPQQFQLTIENIANKYLQNAS

Specific function: Activator for CDC42 by directly engaging this Rho GTPase and acting as potent guanine nucleotide exchange factor (GEF). This activation results in actin cytoskeleton rearrangements and stimulates membrane ruffling, promoting bacterial entry into non- phag

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Note=Secreted via the type III secretion system 1 (SPI-1 TTSS) (By similarity) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GEF (guanine exchange factor) sopE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005414
- InterPro:   IPR016019
- InterPro:   IPR016018 [H]

Pfam domain/function: PF05364 SecIII_SopE_N; PF07487 SopE_GEF [H]

EC number: NA

Molecular weight: Translated: 26448; Mature: 26317

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNITLSTQHYRIHRSDVEPVKEKTTEKDIFAKSITAVRNSFISLSTSLSDRFSLHQQTD
CCCEEECCHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IPTTHFHRGNASEGRAVLTSKTVKDFMLQKLNSLDIKGNASKDPAYARQTCEAILSAVYS
CCCCEECCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHC
NNKDQCCKLLISKGVSITPFLKEIGEAAQNAGLPGEIKNGVFTPGGAGANPFVVPLIASA
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHCCCEECCCCCCCCCEEEEEECCC
SIKYPHMFINHNQQVSFKAYAEKIVMKEVTPLFNKGTMPTPQQFQLTIENIANKYLQNAS
CCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TNITLSTQHYRIHRSDVEPVKEKTTEKDIFAKSITAVRNSFISLSTSLSDRFSLHQQTD
CCEEECCHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IPTTHFHRGNASEGRAVLTSKTVKDFMLQKLNSLDIKGNASKDPAYARQTCEAILSAVYS
CCCCEECCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHC
NNKDQCCKLLISKGVSITPFLKEIGEAAQNAGLPGEIKNGVFTPGGAGANPFVVPLIASA
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHCCCEECCCCCCCCCEEEEEECCC
SIKYPHMFINHNQQVSFKAYAEKIVMKEVTPLFNKGTMPTPQQFQLTIENIANKYLQNAS
CCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA