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The map label for this gene is sseJ

Identifier: 16764975

GI number: 16764975

Start: 1721293

End: 1722519

Strand: Direct

Name: sseJ

Synonym: STM1631

Alternate gene names: 16764975

Gene position: 1721293-1722519 (Clockwise)

Preceding gene: 16764969

Following gene: 16764977

Centisome position: 35.44

GC content: 39.2

Gene sequence:

>1227_bases
ATGCCATTGAGTGTTGGACAGGGTTATTTCACATCATCTATCAGTTCTGAAAAATTTAATGCGATAAAAGAAAGCGCACG
CCTTCCGGAATTAAGTTTATGGGAGAAAATCAAAGCATATTTCTTTACCACCCACCATGCAGAGGCGCTCGAATGTATCT
TTAATCTTTACCACCATCAGGAACTGAATCTAACACCGGTACAGGTTCGCGGAGCCTACATCAAACTTCGAGCCTTAGCG
TCTCAGGGATGTAAAGAACAGTTTATTATAGAATCACAGGAACACGCCGATAAGTTGATTATTAAAGATGATAATGGTGA
AAATATTTTGTCTATTGAGGTTGAATGTCATCCGGAAGCTTTTGGTCTTGCAAAAGAAATCAATAAATCACATCCCAAGC
CCAAAAATATTTCTTTGGGTGATATTACCAGACTGGTATTTTTTGGCGACAGCTTGTCTGACTCCTTAGGGCGTATGTTT
GAAAAAACACATCATATCTTACCCTCCTATGGTCAATACTTTGGCGGAAGGTTTACTAATGGATTTACCTGGACTGAGTT
TTTATCATCTCCACACTTCTTAGGTAAAGAGATGCTTAATTTTGCTGAAGGGGGAAGTACATCGGCAAGCTATTCCTGCT
TTAATTGCATCGGTGACTTTGTATCAAATACGGACAGACAAGTCGCATCTTACACCCCTTCTCACCAGGACCTGGCGATA
TTTTTATTGGGGGCTAATGACTATATGACACTACACAAAGATAATGTAATAATGGTCGTTGAGCAACAAATTGATGATAT
TGAAAAAATAATTTCCGGTGGAGTTAATAATGTTCTGGTCATGGGGATTCCCGATTTGTCTTTAACACCTTATGGCAAAC
ATTCTGATGAAAAAAGAAAGCTTAAGGATGAAAGCATCGCTCACAATGCCCTGTTAAAAACTAATGTTGAAGAATTAAAA
GAAAAATACCCCCAGCATAAAATATGCTATTACGAGACTGCCGATGCATTTAAGGTGATAATGGAGGCGGCCAGTAATAT
TGGTTATGATACGGAAAACCCTTATACTCACCACGGCTATGTACATGTTCCCGGGGCTAAAGACCCTCAGCTAGATATAT
GTCCGCAATACGTCTTCAACGACCTTGTCCATCCAACCCAGGAAGTCCATCATTGTTTTGCCATAATGTTAGAAAGTTTT
ATAGCTCATCATTATTCCACTGAATAA

Upstream 100 bases:

>100_bases
AGCGTAATTCCATATACACCATTTACCTGATTACTTTTCTTGCTAATATTTGCTAATTAATTATTTGCTAAAGCGTGTTT
AATAAAGTAAGGAGGACACT

Downstream 100 bases:

>100_bases
AGTTCCATCGGCTGCGGTACGCCTTGAGCAAAACACAGCTCAATATCATAGTGCCGGTGGCATGAAAATATGATGCTATC
TTTGCTGCCGATTGGTCAAT

Product: secreted effector protein

Products: NA

Alternate protein names: Salmonella-translocated effector J; STE J

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MPLSVGQGYFTSSISSEKFNAIKESARLPELSLWEKIKAYFFTTHHAEALECIFNLYHHQELNLTPVQVRGAYIKLRALA
SQGCKEQFIIESQEHADKLIIKDDNGENILSIEVECHPEAFGLAKEINKSHPKPKNISLGDITRLVFFGDSLSDSLGRMF
EKTHHILPSYGQYFGGRFTNGFTWTEFLSSPHFLGKEMLNFAEGGSTSASYSCFNCIGDFVSNTDRQVASYTPSHQDLAI
FLLGANDYMTLHKDNVIMVVEQQIDDIEKIISGGVNNVLVMGIPDLSLTPYGKHSDEKRKLKDESIAHNALLKTNVEELK
EKYPQHKICYYETADAFKVIMEAASNIGYDTENPYTHHGYVHVPGAKDPQLDICPQYVFNDLVHPTQEVHHCFAIMLESF
IAHHYSTE

Sequences:

>Translated_408_residues
MPLSVGQGYFTSSISSEKFNAIKESARLPELSLWEKIKAYFFTTHHAEALECIFNLYHHQELNLTPVQVRGAYIKLRALA
SQGCKEQFIIESQEHADKLIIKDDNGENILSIEVECHPEAFGLAKEINKSHPKPKNISLGDITRLVFFGDSLSDSLGRMF
EKTHHILPSYGQYFGGRFTNGFTWTEFLSSPHFLGKEMLNFAEGGSTSASYSCFNCIGDFVSNTDRQVASYTPSHQDLAI
FLLGANDYMTLHKDNVIMVVEQQIDDIEKIISGGVNNVLVMGIPDLSLTPYGKHSDEKRKLKDESIAHNALLKTNVEELK
EKYPQHKICYYETADAFKVIMEAASNIGYDTENPYTHHGYVHVPGAKDPQLDICPQYVFNDLVHPTQEVHHCFAIMLESF
IAHHYSTE
>Mature_407_residues
PLSVGQGYFTSSISSEKFNAIKESARLPELSLWEKIKAYFFTTHHAEALECIFNLYHHQELNLTPVQVRGAYIKLRALAS
QGCKEQFIIESQEHADKLIIKDDNGENILSIEVECHPEAFGLAKEINKSHPKPKNISLGDITRLVFFGDSLSDSLGRMFE
KTHHILPSYGQYFGGRFTNGFTWTEFLSSPHFLGKEMLNFAEGGSTSASYSCFNCIGDFVSNTDRQVASYTPSHQDLAIF
LLGANDYMTLHKDNVIMVVEQQIDDIEKIISGGVNNVLVMGIPDLSLTPYGKHSDEKRKLKDESIAHNALLKTNVEELKE
KYPQHKICYYETADAFKVIMEAASNIGYDTENPYTHHGYVHVPGAKDPQLDICPQYVFNDLVHPTQEVHHCFAIMLESFI
AHHYSTE

Specific function: Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is required for endosomal tubulation and negatively regulates the formation of Salmonella-induced filaments (Sifs) in epithelial cells. H

COG id: COG3240

COG function: function code IR; Phospholipase/lecithinase/hemolysin

Gene ontology:

Cell location: Secreted. Host cytoplasm. Note=Secreted via type III secretion system 2 (SPI-2 TTSS), and delivered into the host cytoplasm. Localizes to the Salmonella-containing vacuole (SCV) at early time points following invasion and subsequently traffics away from t

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSEJ_SALTY (Q9FD10)

Other databases:

- EMBL:   AF294582
- EMBL:   AE006468
- RefSeq:   NP_460590.1
- ProteinModelPortal:   Q9FD10
- PRIDE:   Q9FD10
- GeneID:   1253149
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM1631
- HOGENOM:   HBG671276
- OMA:   ALECIFK
- ProtClustDB:   PRK15381
- BioCyc:   STYP99287:STM1631-MONOMER
- GO:   GO:0009405
- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR010637
- Gene3D:   G3DSA:3.40.50.1110

Pfam domain/function: PF00657 Lipase_GDSL; PF06767 Sif; SSF52266 Esterase_SGNH_hydro-type

EC number: NA

Molecular weight: Translated: 46077; Mature: 45946

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS01098 LIPASE_GDSL_SER

Important sites: ACT_SITE 151-151 ACT_SITE 381-381 ACT_SITE 384-384

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSVGQGYFTSSISSEKFNAIKESARLPELSLWEKIKAYFFTTHHAEALECIFNLYHHQ
CCCCCCCCCHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHEECCHHHHHHHHHHHHHCC
ELNLTPVQVRGAYIKLRALASQGCKEQFIIESQEHADKLIIKDDNGENILSIEVECHPEA
CCCCCEEEECCCEEEEEEHHHCCCCHHHCCCCCCCCCEEEEECCCCCEEEEEEEEECCHH
FGLAKEINKSHPKPKNISLGDITRLVFFGDSLSDSLGRMFEKTHHILPSYGQYFGGRFTN
HHHHHHHHHCCCCCCCCCHHHHEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCC
GFTWTEFLSSPHFLGKEMLNFAEGGSTSASYSCFNCIGDFVSNTDRQVASYTPSHQDLAI
CCCHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCEEE
FLLGANDYMTLHKDNVIMVVEQQIDDIEKIISGGVNNVLVMGIPDLSLTPYGKHSDEKRK
EEECCCCEEEEECCCEEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHH
LKDESIAHNALLKTNVEELKEKYPQHKICYYETADAFKVIMEAASNIGYDTENPYTHHGY
HHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCEECCCE
VHVPGAKDPQLDICPQYVFNDLVHPTQEVHHCFAIMLESFIAHHYSTE
EECCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PLSVGQGYFTSSISSEKFNAIKESARLPELSLWEKIKAYFFTTHHAEALECIFNLYHHQ
CCCCCCCCHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHEECCHHHHHHHHHHHHHCC
ELNLTPVQVRGAYIKLRALASQGCKEQFIIESQEHADKLIIKDDNGENILSIEVECHPEA
CCCCCEEEECCCEEEEEEHHHCCCCHHHCCCCCCCCCEEEEECCCCCEEEEEEEEECCHH
FGLAKEINKSHPKPKNISLGDITRLVFFGDSLSDSLGRMFEKTHHILPSYGQYFGGRFTN
HHHHHHHHHCCCCCCCCCHHHHEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCC
GFTWTEFLSSPHFLGKEMLNFAEGGSTSASYSCFNCIGDFVSNTDRQVASYTPSHQDLAI
CCCHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCEEE
FLLGANDYMTLHKDNVIMVVEQQIDDIEKIISGGVNNVLVMGIPDLSLTPYGKHSDEKRK
EEECCCCEEEEECCCEEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHH
LKDESIAHNALLKTNVEELKEKYPQHKICYYETADAFKVIMEAASNIGYDTENPYTHHGY
HHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCEECCCE
VHVPGAKDPQLDICPQYVFNDLVHPTQEVHHCFAIMLESFIAHHYSTE
EECCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10861017; 11677609