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The map label for this gene is narU

Identifier: 16764920

GI number: 16764920

Start: 1659208

End: 1660596

Strand: Direct

Name: narU

Synonym: STM1576

Alternate gene names: 16764920

Gene position: 1659208-1660596 (Clockwise)

Preceding gene: 16764919

Following gene: 16764921

Centisome position: 34.16

GC content: 52.63

Gene sequence:

>1389_bases
ATGACACGACAAAACGAGAATTATAACCGTTATCTCTTGAGTGACTGGCGACCCGAAAATCCAGCCTTCTGGGAGAATAA
AGGGAAAGGCATCGCCAGAAGAAACTTATGGATTTCAGTAAGTTGTTTGCTCCTGGCATTTTGCGTCTGGATGTTGTTTA
GCGCTGTTGCCGTCAACCTCAATAAAATTGGATTTAATTTTACCACCGACCAACTCTTTTTATTAACCGCATTACCTTCT
CTTTCCGGCGCAATATTACGCGTTCCCTACTCCTTTATGGTGCCACTGTTTGGTGGTCGTAAATGGACCGTATTAAGCAC
CGTAATATTGATTATTCCCTGCGCCTGGCTGGGATTCGCCGTACAAAATCCCGCCACCCCTTTCGGGGTATTTATGCTCA
TCGCTCTGTTGTGCGGTTTTGCGGGGGCAAACTTCGCGTCCAGTATGGGCAACATCAGTTTTTTCTTTCCAAAAGCCAGA
CAAGGTAGCGCGTTGGGTATTAACGGCGGACTGGGAAATCTTGGCGTCAGTGTGATGCAGTTGATTGCGCCGCTGGTTAT
TTTTCTGCCGATATTTACTTTTTTAGGCGTCCAGGGCGTGCCGCAACCTGATGGCTCCCTGCTCGCCCTCACTAACGCCG
CCTGGATCTGGGTGCCGTTACTGGCTGTCGCGACGCTTGCCGCCTGGTTTGGTATGAACGATATCGGCAGTTCAAAAGCG
TCTGTCGCCTCGCAATTGCCGGTGCTAAAACGTCTGCACCTGTGGCTGCTTAGCCTGCTGTACCTGGCGACATTCGGGTC
ATTCATCGGCTTTTCCGCAGGTTTCGCCATGCTCGCCAAAACGCAATTTCCCGACGTAAACATTCTGCAACTGGCGTTCT
TTGGCCCCTTTATCGGCGCGCTGGCGCGATCGGCAGGCGGCGTGATCTCCGATAAATTCGGCGGCGTTCGCGTAACGCTC
ATTAATTTTATCTTTATGGCGCTGTTTACCGCCTTGCTATTCCTCACGCTGCCAGGCTCCGGCGCAGGCAGCTTTAGCGC
GTTCTATCTTGTTTTTATGGGCCTGTTTTTGACAGCGGGTCTCGGCAGCGGTTCTACCTTCCAGATGATTGCGGTGATCT
TCCGGCAGATCACGCTCTACAACGTCAAACTGCGCGGCGGGAGCGATGAGCAGGCGCAGCGCGAAGCGGTGACGGATACC
GCCGCCGCGCTCGGTTTTATCTCCGCTATTGGCGCTGTGGGCGGCTTCTTTATTCCAAAAGCGTTTGGCACCTCTTTGGC
CTTGACCGGCTCGCCGGTGGGCGCCATGAAAATCTTTTTGCTGTTTTACCTCGCTTGCGTCCTGCTGACCTGGCTGGTCT
ACGGGCGTCGTAAGCCTAAACAACAATAA

Upstream 100 bases:

>100_bases
AATATTCTAAAAAAACTTCAATAAGTTCTCAAAAGAGCGCGTGACGCTCTGTCTCTTTTTTGTGTTTTTCTTCAAATGAT
ATGCGCATGTGAGGGGTAAA

Downstream 100 bases:

>100_bases
CAACATACGCACCTGTAGGACGGCCATCCGGTAATGGTTTAACGCCAAATCGACAGGATGGCGGAAAATTTATCGAAGCA
GGAGAAATGTCATGAGTAAA

Product: nitrate extrusion protein

Products: Proton [Cytoplasm]; nitrite [Periplasm] [C]

Alternate protein names: Nitrite facilitator 2

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MTRQNENYNRYLLSDWRPENPAFWENKGKGIARRNLWISVSCLLLAFCVWMLFSAVAVNLNKIGFNFTTDQLFLLTALPS
LSGAILRVPYSFMVPLFGGRKWTVLSTVILIIPCAWLGFAVQNPATPFGVFMLIALLCGFAGANFASSMGNISFFFPKAR
QGSALGINGGLGNLGVSVMQLIAPLVIFLPIFTFLGVQGVPQPDGSLLALTNAAWIWVPLLAVATLAAWFGMNDIGSSKA
SVASQLPVLKRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILQLAFFGPFIGALARSAGGVISDKFGGVRVTL
INFIFMALFTALLFLTLPGSGAGSFSAFYLVFMGLFLTAGLGSGSTFQMIAVIFRQITLYNVKLRGGSDEQAQREAVTDT
AAALGFISAIGAVGGFFIPKAFGTSLALTGSPVGAMKIFLLFYLACVLLTWLVYGRRKPKQQ

Sequences:

>Translated_462_residues
MTRQNENYNRYLLSDWRPENPAFWENKGKGIARRNLWISVSCLLLAFCVWMLFSAVAVNLNKIGFNFTTDQLFLLTALPS
LSGAILRVPYSFMVPLFGGRKWTVLSTVILIIPCAWLGFAVQNPATPFGVFMLIALLCGFAGANFASSMGNISFFFPKAR
QGSALGINGGLGNLGVSVMQLIAPLVIFLPIFTFLGVQGVPQPDGSLLALTNAAWIWVPLLAVATLAAWFGMNDIGSSKA
SVASQLPVLKRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILQLAFFGPFIGALARSAGGVISDKFGGVRVTL
INFIFMALFTALLFLTLPGSGAGSFSAFYLVFMGLFLTAGLGSGSTFQMIAVIFRQITLYNVKLRGGSDEQAQREAVTDT
AAALGFISAIGAVGGFFIPKAFGTSLALTGSPVGAMKIFLLFYLACVLLTWLVYGRRKPKQQ
>Mature_461_residues
TRQNENYNRYLLSDWRPENPAFWENKGKGIARRNLWISVSCLLLAFCVWMLFSAVAVNLNKIGFNFTTDQLFLLTALPSL
SGAILRVPYSFMVPLFGGRKWTVLSTVILIIPCAWLGFAVQNPATPFGVFMLIALLCGFAGANFASSMGNISFFFPKARQ
GSALGINGGLGNLGVSVMQLIAPLVIFLPIFTFLGVQGVPQPDGSLLALTNAAWIWVPLLAVATLAAWFGMNDIGSSKAS
VASQLPVLKRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILQLAFFGPFIGALARSAGGVISDKFGGVRVTLI
NFIFMALFTALLFLTLPGSGAGSFSAFYLVFMGLFLTAGLGSGSTFQMIAVIFRQITLYNVKLRGGSDEQAQREAVTDTA
AALGFISAIGAVGGFFIPKAFGTSLALTGSPVGAMKIFLLFYLACVLLTWLVYGRRKPKQQ

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family

Homologues:

Organism=Escherichia coli, GI1787743, Length=462, Percent_Identity=85.0649350649351, Blast_Score=751, Evalue=0.0,
Organism=Escherichia coli, GI1787475, Length=456, Percent_Identity=75.4385964912281, Blast_Score=678, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NARU_SALTY (P37593)

Other databases:

- EMBL:   AE006468
- EMBL:   D26057
- RefSeq:   NP_460535.1
- ProteinModelPortal:   P37593
- GeneID:   1253094
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM1576
- NMPDR:   fig|99287.1.peg.1525
- HOGENOM:   HBG307423
- OMA:   VMQMVAP
- ProtClustDB:   PRK15034
- BioCyc:   STYP99287:STM1576-MONOMER
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737
- TIGRFAMs:   TIGR00886

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 49752; Mature: 49620

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe9306e4)-; HASH(0xd84d0a8)-; HASH(0xeeb2d98)-; HASH(0xeeb7b7c)-; HASH(0xea68354)-; HASH(0xee97cf4)-; HASH(0xec795a0)-; HASH(0xea1cc6c)-; HASH(0xee9dc40)-; HASH(0xeacdc90)-; HASH(0xe72d874)-; HASH(0xd84d00c)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRQNENYNRYLLSDWRPENPAFWENKGKGIARRNLWISVSCLLLAFCVWMLFSAVAVNL
CCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKIGFNFTTDQLFLLTALPSLSGAILRVPYSFMVPLFGGRKWTVLSTVILIIPCAWLGFA
HHCCCCCCCHHHHHHHHHCCCCCHHEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VQNPATPFGVFMLIALLCGFAGANFASSMGNISFFFPKARQGSALGINGGLGNLGVSVMQ
HCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCCEEEECCCCHHHHHHHHH
LIAPLVIFLPIFTFLGVQGVPQPDGSLLALTNAAWIWVPLLAVATLAAWFGMNDIGSSKA
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
SVASQLPVLKRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILQLAFFGPFIGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LARSAGGVISDKFGGVRVTLINFIFMALFTALLFLTLPGSGAGSFSAFYLVFMGLFLTAG
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC
LGSGSTFQMIAVIFRQITLYNVKLRGGSDEQAQREAVTDTAAALGFISAIGAVGGFFIPK
CCCCCHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGTSLALTGSPVGAMKIFLLFYLACVLLTWLVYGRRKPKQQ
HHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
>Mature Secondary Structure 
TRQNENYNRYLLSDWRPENPAFWENKGKGIARRNLWISVSCLLLAFCVWMLFSAVAVNL
CCCCCCCCCEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKIGFNFTTDQLFLLTALPSLSGAILRVPYSFMVPLFGGRKWTVLSTVILIIPCAWLGFA
HHCCCCCCCHHHHHHHHHCCCCCHHEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VQNPATPFGVFMLIALLCGFAGANFASSMGNISFFFPKARQGSALGINGGLGNLGVSVMQ
HCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCCEEEECCCCHHHHHHHHH
LIAPLVIFLPIFTFLGVQGVPQPDGSLLALTNAAWIWVPLLAVATLAAWFGMNDIGSSKA
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
SVASQLPVLKRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILQLAFFGPFIGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LARSAGGVISDKFGGVRVTLINFIFMALFTALLFLTLPGSGAGSFSAFYLVFMGLFLTAG
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC
LGSGSTFQMIAVIFRQITLYNVKLRGGSDEQAQREAVTDTAAALGFISAIGAVGGFFIPK
CCCCCHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGTSLALTGSPVGAMKIFLLFYLACVLLTWLVYGRRKPKQQ
HHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: nitrite [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + nitrite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677609; 7926834