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The map label for this gene is smvA

Identifier: 16764918

GI number: 16764918

Start: 1656810

End: 1658297

Strand: Reverse

Name: smvA

Synonym: STM1574

Alternate gene names: 16764918

Gene position: 1658297-1656810 (Counterclockwise)

Preceding gene: 16764926

Following gene: 16764914

Centisome position: 34.14

GC content: 57.8

Gene sequence:

>1488_bases
ATGTTTCGTCAGTGGTTAACGTTAGTCATTATTGTGCTGGTCTATATTCCTGTCGCCATTGATGCCACGGTCTTGCATGT
CGCCGCGCCGACACTGAGTATGACACTGGGGGCCAGCGGCAACGAGCTGCTGTGGATCATTGATATTTATTCTTTGGTCA
TGGCTGGCATGGTGTTGCCGATGGGCGCACTTGGCGATCGTATCGGTTTTAAACGCCTGCTGATGCTGGGCGGGACGCTC
TTTGGCCTGGCGTCATTGGCGGCAGCGTTTTCGCATACCGCCAGTTGGCTTATCGCCACCAGGGTATTACTGGCTATTGG
CGCGGCGATGATTGTACCGGCGACGCTGGCCGGAATACGCGCTACCTTTTGTGAAGAGAAGCATCGCAACATGGCGCTGG
GCGTCTGGGCAGCGGTAGGTTCGGGCGGAGCGGCGTTTGGGCCGCTCATCGGCGGCATATTATTAGAGCATTTTTACTGG
GGATCGGTTTTCCTGATCAACGTGCCGATTGTGCTGGTCGTCATGGGCTTAACCGCCCGCTATGTTCCTCGCCAGGCGGG
CCGTCGCGATCAACCGCTCAATCTTGGCCATGCGGTGATGCTGATTATTGCCATTTTGCTGTTGGTCTATAGCGCTAAAA
CCGCGCTGAAAGGGCATCTGTCGCTGTGGGTTATCTCGTTTACCCTGCTTACCGGCGCGCTGCTCCTGGGACTCTTTATC
CGCACACAGCTTGCGACATCGCGTCCGATGATTGATATGCGACTATTTACCCATCGCATTATCCTGAGCGGCGTCGTGAT
GGCGATGACCGCGATGATCACTCTGGTGGGTTTTGAGCTGCTGATGGCGCAAGAGCTGCAGTTTGTTCACGGGCTATCGC
CTTATGAGGCCGGGGTATTTATGCTGCCGGTAATGGTCGCCAGTGGATTCAGCGGGCCGATTGCGGGCGTGCTGGTCTCG
CGTCTGGGACTACGGCTGGTCGCGACGGGCGGCATGGCGTTAAGCGCGCTGAGTTTTTATGGCCTGGCGATGACGGATTT
CAGCACCCAACAATGGCAGGCCTGGGGGCTGATGGCGCTGCTGGGATTTAGCGCCGCCAGCGCATTGCTGGCTTCCACGT
CGGCAATTATGGCCGCTGCGCCGGCAGAAAAAGCGGCGGCGGCCGGCGCGATAGAAACGATGGCTTATGAACTGGGCGCG
GGACTGGGCATTGCCATTTTCGGTCTGTTGTTAAGCCGTAGCTTCTCCGCGTCTATCCGTCTGCCTGCCGGGCTTGAGGC
GCAAGAGATTGCCAGAGCGTCATCTTCAATGGGAGAAGCCGTGCAGTTGGCGAATAGCCTACCGCCCACGCAGGGGCAGG
CAATACTGGACGCCGCCAGACATGCCTTTATCTGGTCGCATAGCGTGGCGTTAAGCAGCGCCGGGAGTATGCTTCTTTTG
CTGGCGGTAGGGATGTGGTTCAGCCTGGCAAAAGCCCAACGCCGATAA

Upstream 100 bases:

>100_bases
CAATGCGCGCAGTGTAACAAAAAGCTGGACATTCGTTCAACTTACCGTAGGATCCTGAAGCTGGACAAGCGTCCAAATTT
GAGTTTTTGAAGGGAGAGTT

Downstream 100 bases:

>100_bases
CATAACGCCTGGTGAGATAAGCGCTTAATGCGCTAGCTGGCAGGGGGAGTCCATTTCCTCAACCTCTTTACCGCTGAGGC
GTGCGTATTGCCCCGTCAGT

Product: methyl viologen resistance

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MFRQWLTLVIIVLVYIPVAIDATVLHVAAPTLSMTLGASGNELLWIIDIYSLVMAGMVLPMGALGDRIGFKRLLMLGGTL
FGLASLAAAFSHTASWLIATRVLLAIGAAMIVPATLAGIRATFCEEKHRNMALGVWAAVGSGGAAFGPLIGGILLEHFYW
GSVFLINVPIVLVVMGLTARYVPRQAGRRDQPLNLGHAVMLIIAILLLVYSAKTALKGHLSLWVISFTLLTGALLLGLFI
RTQLATSRPMIDMRLFTHRIILSGVVMAMTAMITLVGFELLMAQELQFVHGLSPYEAGVFMLPVMVASGFSGPIAGVLVS
RLGLRLVATGGMALSALSFYGLAMTDFSTQQWQAWGLMALLGFSAASALLASTSAIMAAAPAEKAAAAGAIETMAYELGA
GLGIAIFGLLLSRSFSASIRLPAGLEAQEIARASSSMGEAVQLANSLPPTQGQAILDAARHAFIWSHSVALSSAGSMLLL
LAVGMWFSLAKAQRR

Sequences:

>Translated_495_residues
MFRQWLTLVIIVLVYIPVAIDATVLHVAAPTLSMTLGASGNELLWIIDIYSLVMAGMVLPMGALGDRIGFKRLLMLGGTL
FGLASLAAAFSHTASWLIATRVLLAIGAAMIVPATLAGIRATFCEEKHRNMALGVWAAVGSGGAAFGPLIGGILLEHFYW
GSVFLINVPIVLVVMGLTARYVPRQAGRRDQPLNLGHAVMLIIAILLLVYSAKTALKGHLSLWVISFTLLTGALLLGLFI
RTQLATSRPMIDMRLFTHRIILSGVVMAMTAMITLVGFELLMAQELQFVHGLSPYEAGVFMLPVMVASGFSGPIAGVLVS
RLGLRLVATGGMALSALSFYGLAMTDFSTQQWQAWGLMALLGFSAASALLASTSAIMAAAPAEKAAAAGAIETMAYELGA
GLGIAIFGLLLSRSFSASIRLPAGLEAQEIARASSSMGEAVQLANSLPPTQGQAILDAARHAFIWSHSVALSSAGSMLLL
LAVGMWFSLAKAQRR
>Mature_495_residues
MFRQWLTLVIIVLVYIPVAIDATVLHVAAPTLSMTLGASGNELLWIIDIYSLVMAGMVLPMGALGDRIGFKRLLMLGGTL
FGLASLAAAFSHTASWLIATRVLLAIGAAMIVPATLAGIRATFCEEKHRNMALGVWAAVGSGGAAFGPLIGGILLEHFYW
GSVFLINVPIVLVVMGLTARYVPRQAGRRDQPLNLGHAVMLIIAILLLVYSAKTALKGHLSLWVISFTLLTGALLLGLFI
RTQLATSRPMIDMRLFTHRIILSGVVMAMTAMITLVGFELLMAQELQFVHGLSPYEAGVFMLPVMVASGFSGPIAGVLVS
RLGLRLVATGGMALSALSFYGLAMTDFSTQQWQAWGLMALLGFSAASALLASTSAIMAAAPAEKAAAAGAIETMAYELGA
GLGIAIFGLLLSRSFSASIRLPAGLEAQEIARASSSMGEAVQLANSLPPTQGQAILDAARHAFIWSHSVALSSAGSMLLL
LAVGMWFSLAKAQRR

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family

Homologues:

Organism=Escherichia coli, GI1789042, Length=395, Percent_Identity=26.5822784810127, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1788710, Length=391, Percent_Identity=27.3657289002558, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1790195, Length=399, Percent_Identity=28.0701754385965, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI87081983, Length=399, Percent_Identity=26.0651629072682, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788392, Length=409, Percent_Identity=25.1833740831296, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322958, Length=515, Percent_Identity=23.3009708737864, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SMVA_SALTY (P37594)

Other databases:

- EMBL:   D26057
- EMBL:   AE006468
- RefSeq:   NP_460533.1
- ProteinModelPortal:   P37594
- GeneID:   1253092
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM1574
- NMPDR:   fig|99287.1.peg.1523
- HOGENOM:   HBG679341
- OMA:   WVISFTL
- ProtClustDB:   PRK14995
- BioCyc:   STYP99287:STM1574-MONOMER
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411
- PRINTS:   PR01036

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 52135; Mature: 52135

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xebaee14)-; HASH(0xee7d390)-; HASH(0xc2cb61c)-; HASH(0xee46ca0)-; HASH(0xe3376b0)-; HASH(0xea79758)-; HASH(0xee8f008)-; HASH(0xd4ca2e8)-; HASH(0xdf188f0)-; HASH(0xceb7b20)-; HASH(0xee7b7d0)-; HASH(0xee7b818)-; HASH(0xee917bc)-; HASH(0xee91858)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRQWLTLVIIVLVYIPVAIDATVLHVAAPTLSMTLGASGNELLWIIDIYSLVMAGMVLP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCEEEHHHHHHHHHHHHHHC
MGALGDRIGFKRLLMLGGTLFGLASLAAAFSHTASWLIATRVLLAIGAAMIVPATLAGIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATFCEEKHRNMALGVWAAVGSGGAAFGPLIGGILLEHFYWGSVFLINVPIVLVVMGLTAR
HHHHHHHHCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
YVPRQAGRRDQPLNLGHAVMLIIAILLLVYSAKTALKGHLSLWVISFTLLTGALLLGLFI
HCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTQLATSRPMIDMRLFTHRIILSGVVMAMTAMITLVGFELLMAQELQFVHGLSPYEAGVF
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCHHH
MLPVMVASGFSGPIAGVLVSRLGLRLVATGGMALSALSFYGLAMTDFSTQQWQAWGLMAL
HHHHHHHCCCCCHHHHHHHHHHCHHEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LGFSAASALLASTSAIMAAAPAEKAAAAGAIETMAYELGAGLGIAIFGLLLSRSFSASIR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEE
LPAGLEAQEIARASSSMGEAVQLANSLPPTQGQAILDAARHAFIWSHSVALSSAGSMLLL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LAVGMWFSLAKAQRR
HHHHHHHHHHHHHCH
>Mature Secondary Structure
MFRQWLTLVIIVLVYIPVAIDATVLHVAAPTLSMTLGASGNELLWIIDIYSLVMAGMVLP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCEEEHHHHHHHHHHHHHHC
MGALGDRIGFKRLLMLGGTLFGLASLAAAFSHTASWLIATRVLLAIGAAMIVPATLAGIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATFCEEKHRNMALGVWAAVGSGGAAFGPLIGGILLEHFYWGSVFLINVPIVLVVMGLTAR
HHHHHHHHCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
YVPRQAGRRDQPLNLGHAVMLIIAILLLVYSAKTALKGHLSLWVISFTLLTGALLLGLFI
HCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTQLATSRPMIDMRLFTHRIILSGVVMAMTAMITLVGFELLMAQELQFVHGLSPYEAGVF
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCHHH
MLPVMVASGFSGPIAGVLVSRLGLRLVATGGMALSALSFYGLAMTDFSTQQWQAWGLMAL
HHHHHHHCCCCCHHHHHHHHHHCHHEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LGFSAASALLASTSAIMAAAPAEKAAAAGAIETMAYELGAGLGIAIFGLLLSRSFSASIR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEE
LPAGLEAQEIARASSSMGEAVQLANSLPPTQGQAILDAARHAFIWSHSVALSSAGSMLLL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LAVGMWFSLAKAQRR
HHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7926834; 11677609