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The map label for this gene is galS [H]

Identifier: 16764899

GI number: 16764899

Start: 1632130

End: 1633137

Strand: Direct

Name: galS [H]

Synonym: STM1555

Alternate gene names: 16764899

Gene position: 1632130-1633137 (Clockwise)

Preceding gene: 16764897

Following gene: 16764900

Centisome position: 33.6

GC content: 53.97

Gene sequence:

>1008_bases
ATGGCGACAATAAAGGATGTTGCATCCATGGCCGGTGTATCAACCGCGACTGTATCACGCGTCATTAACCAGACCGCGTG
GGTTGAACCCGTCACGCGCGAACGTGTAGAGAAAGCCATGCGCGATCTCAATTATCGCCGGAATGCCGCCGCTATCGCGC
TGGCGAAACGCAGCGGCGATATGCTCGGATTGTTAACCGGCAATCTGGCTGATCCTTTTTTTGCCCGCCTTGCTCGCGGC
GTAGAAGATGTTTCTCGTAAGCAGCAGTATCGCTTGATGGTATGTAGCGGCGGCCACGACGAAGAAATGGAAAAAGCGGG
ACTGGATTTTCTGGTTAATCAAGGTTGTGAGGCTATTGTTGTACACGCCAGCCGATTACCGGATAAAGAGCTGCTGCGCT
ACGCAGCCCACTTTCCAGCGCTGGTTGTTGTGAATCGTTATATAGCCGGTATGGCCAACCGGTGTATCTGGCTGGAAAAT
CGCAGCGCCGCCAGGGAAGCGACCCGATATCTCCTGGCGAACGGGCATCGGCGCATTGCCTGTGTGACATCTGACTTGCC
AATTATTGATCGTCAGGAACGCCTTGATGGTTACCGTGAGGCGCTGGAAGAATATGGTATTTCTCCAGACCCTCGCTGGG
TGATTAGCGTTCCGTTTAACGAAGAAGGCGGCGAGCGGGCCGCGCATCAACTTATTAATAGCGGGCTTCCCCTTACGGCA
GCGGTCACGTTTAACGATGTGATGGCCGCTGGCATTATGCGTATTCTTCATCAACGCGGCGTTCAGCTTCCGCAACAGCT
CTCTATTGTCGGCTTTGATGATGTGGTGCTGGCCCGGTATCTTTATCCGGCGTTAACAACAATGCATTATCCGGTCGAAC
AAATGGCGCGTTGTGCCGCGCAACTGGCGATACAGTTATACCAGGGCATTACGCCGCCGCCCAGCAGCAATCATTTCAAT
GCCGAACTGGTGATCCGCGATTCTGTCGCGCCCTATTTTTCACGGTGA

Upstream 100 bases:

>100_bases
TTTATAAATTCAGGCTGCGCCGCAGTGATTGCACTGATTTCCACGTAACATGATAATCAATGGCTTTGATACCCTTTAAA
CGAACAAATATGAGCTTAAC

Downstream 100 bases:

>100_bases
ATGCGTCGTCAACAGTAAAAACTGTGATGGCTATCAAATTAACCCTTCTCATGTAATCGGTTACATGGACGAAAGTAATG
GATCACAGTAGTGGGAAGCG

Product: transcriptional regulator

Products: NA

Alternate protein names: Mgl repressor and galactose ultrainduction factor [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MATIKDVASMAGVSTATVSRVINQTAWVEPVTRERVEKAMRDLNYRRNAAAIALAKRSGDMLGLLTGNLADPFFARLARG
VEDVSRKQQYRLMVCSGGHDEEMEKAGLDFLVNQGCEAIVVHASRLPDKELLRYAAHFPALVVVNRYIAGMANRCIWLEN
RSAAREATRYLLANGHRRIACVTSDLPIIDRQERLDGYREALEEYGISPDPRWVISVPFNEEGGERAAHQLINSGLPLTA
AVTFNDVMAAGIMRILHQRGVQLPQQLSIVGFDDVVLARYLYPALTTMHYPVEQMARCAAQLAIQLYQGITPPPSSNHFN
AELVIRDSVAPYFSR

Sequences:

>Translated_335_residues
MATIKDVASMAGVSTATVSRVINQTAWVEPVTRERVEKAMRDLNYRRNAAAIALAKRSGDMLGLLTGNLADPFFARLARG
VEDVSRKQQYRLMVCSGGHDEEMEKAGLDFLVNQGCEAIVVHASRLPDKELLRYAAHFPALVVVNRYIAGMANRCIWLEN
RSAAREATRYLLANGHRRIACVTSDLPIIDRQERLDGYREALEEYGISPDPRWVISVPFNEEGGERAAHQLINSGLPLTA
AVTFNDVMAAGIMRILHQRGVQLPQQLSIVGFDDVVLARYLYPALTTMHYPVEQMARCAAQLAIQLYQGITPPPSSNHFN
AELVIRDSVAPYFSR
>Mature_334_residues
ATIKDVASMAGVSTATVSRVINQTAWVEPVTRERVEKAMRDLNYRRNAAAIALAKRSGDMLGLLTGNLADPFFARLARGV
EDVSRKQQYRLMVCSGGHDEEMEKAGLDFLVNQGCEAIVVHASRLPDKELLRYAAHFPALVVVNRYIAGMANRCIWLENR
SAAREATRYLLANGHRRIACVTSDLPIIDRQERLDGYREALEEYGISPDPRWVISVPFNEEGGERAAHQLINSGLPLTAA
VTFNDVMAAGIMRILHQRGVQLPQQLSIVGFDDVVLARYLYPALTTMHYPVEQMARCAAQLAIQLYQGITPPPSSNHFNA
ELVIRDSVAPYFSR

Specific function: Repressor of the mgl operon. Binds galactose and D- fucose as inducers. GalS binds to an operator DNA sequence within its own coding sequence (corresponding to residues 15 to 20) [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788474, Length=330, Percent_Identity=42.1212121212121, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1789202, Length=330, Percent_Identity=42.1212121212121, Blast_Score=254, Evalue=5e-69,
Organism=Escherichia coli, GI1790369, Length=330, Percent_Identity=31.8181818181818, Blast_Score=184, Evalue=6e-48,
Organism=Escherichia coli, GI1789068, Length=336, Percent_Identity=31.8452380952381, Blast_Score=177, Evalue=8e-46,
Organism=Escherichia coli, GI1790194, Length=309, Percent_Identity=32.6860841423948, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1787948, Length=332, Percent_Identity=31.0240963855422, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1787906, Length=289, Percent_Identity=29.0657439446367, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=25.3125, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786540, Length=317, Percent_Identity=29.0220820189274, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=26.0450160771704, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI48994940, Length=318, Percent_Identity=24.5283018867925, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789456, Length=320, Percent_Identity=24.6875, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786268, Length=293, Percent_Identity=27.3037542662116, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37250; Mature: 37119

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVASMAGVSTATVSRVINQTAWVEPVTRERVEKAMRDLNYRRNAAAIALAKRSGD
CCCHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHEEEECCCCC
MLGLLTGNLADPFFARLARGVEDVSRKQQYRLMVCSGGHDEEMEKAGLDFLVNQGCEAIV
EEEEEECCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHCCCCEEE
VHASRLPDKELLRYAAHFPALVVVNRYIAGMANRCIWLENRSAAREATRYLLANGHRRIA
EEHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHCEEEECCCHHHHHHHHHHHHCCCEEEE
CVTSDLPIIDRQERLDGYREALEEYGISPDPRWVISVPFNEEGGERAAHQLINSGLPLTA
EEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEE
AVTFNDVMAAGIMRILHQRGVQLPQQLSIVGFDDVVLARYLYPALTTMHYPVEQMARCAA
EEHHHHHHHHHHHHHHHHHCCCCCHHHEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QLAIQLYQGITPPPSSNHFNAELVIRDSVAPYFSR
HHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
ATIKDVASMAGVSTATVSRVINQTAWVEPVTRERVEKAMRDLNYRRNAAAIALAKRSGD
CCHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHEEEECCCCC
MLGLLTGNLADPFFARLARGVEDVSRKQQYRLMVCSGGHDEEMEKAGLDFLVNQGCEAIV
EEEEEECCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHCCCCEEE
VHASRLPDKELLRYAAHFPALVVVNRYIAGMANRCIWLENRSAAREATRYLLANGHRRIA
EEHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHCEEEECCCHHHHHHHHHHHHCCCEEEE
CVTSDLPIIDRQERLDGYREALEEYGISPDPRWVISVPFNEEGGERAAHQLINSGLPLTA
EEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEE
AVTFNDVMAAGIMRILHQRGVQLPQQLSIVGFDDVVLARYLYPALTTMHYPVEQMARCAA
EEHHHHHHHHHHHHHHHHHCCCCCHHHEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QLAIQLYQGITPPPSSNHFNAELVIRDSVAPYFSR
HHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1619663; 9278503; 8208287; 8982002 [H]