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The map label for this gene is fumA

Identifier: 16764814

GI number: 16764814

Start: 1542187

End: 1543929

Strand: Direct

Name: fumA

Synonym: STM1468

Alternate gene names: 16764814

Gene position: 1542187-1543929 (Clockwise)

Preceding gene: 39546318

Following gene: 16764815

Centisome position: 31.75

GC content: 54.68

Gene sequence:

>1743_bases
ATGTCAAACAAACCCTTCTTTTATCAAGACCCTTTTCCCCTCAAAAAGGACGATACCGAGTACTACTTGTTGACCAGCGA
ACATGTTTCCGTCGCCGAATTCGAAGGACAAGAGATCCTGAAAGTCGCGCCGGAAGCGCTAACGCTGTTGGCGCGTCAGG
CCTTCCACGATGCGTCTTTTATGCTACGTCCCGCGCATCAGCAGCAGGTGGCGGATATTCTGCGCGATCCGCAGGCCAGT
GAAAACGACAAATATGTCGCGCTGCAATTCCTGCGAAACTCCGATATCGCGGCCAAGGGCGTGTTGCCCACCTGCCAGGA
TACCGGTACGGCCATTATTGTCGGCAAAAAAGGTCAACGCGTCTGGACTGGTGGAGGTGATGAAGCGGCGCTGGCTCGCG
GCGTATATAACACCTATATCGAAGATAACCTGCGCTATTCACAGAACGCGGCGCTGGATATGTACAAAGAGGTCAATACC
GGTACTAACCTGCCGGCGCAGATCGATCTCTACAGCGTCGATGGCGATGAATATAAATTCCTCTGTATCGCTAAAGGCGG
CGGATCGGCGAATAAAACCTACCTCTATCAGGAGACAAAAGCGCTTCTGACGCCGGGTAAGCTTAAAAATTATCTCGTCG
ATAAGATGCGTACGCTGGGCACCGCCGCTTGTCCGCCGTATCACATCGCGTTTGTCATTGGCGGCACCTCGGCGGAAGCG
AACCTGAAGACCGTTAAGCTGGCCTCGGCAAAATACTATGATGCGCTACCAACCGAAGGCAATGAACACGGGCAGGCCTT
CCGCGATATTGAGCTGGAAAAAGAATTGTTGCTTGAGGCGCAAAATCTTGGCTTAGGCGCGCAGTTCGGCGGCAAGTATT
TCGCTCACGATATTCGTGTCATCCGATTGCCGCGCCACGGGGCATCGTGTCCGGTAGGTATGGGCGTGTCCTGCTCCGCG
GATCGAAACATTAAAGCGAAGATCAACCGGGACGGGATCTGGATCGAGAAGCTGGAGCGCAATCCCGGTAAATATATTCC
AGAGGCGCTGCGCCAGGCGGGAGAGGGTGAGGCGGTGCGCGTTGATCTTAACCGTCCCATGAGCGAGATACTGCAACAAC
TGTCGCAGTATCCGGTATCAACGCGCCTGTCGCTGAACGGCACGATTATTGTGGGCCGCGACATCGCGCACGCTAAACTG
AAAGAACGGATGGACAGAGGCGAAGGTTTGCCGCAGTACATCAAAGATCATCCTATCTATTATGCGGGGCCGGCAAAAAC
GCCGGAAGGGTATGCCTCCGGGTCGCTTGGGCCGACTACCGCAGGACGAATGGACTCTTATGTTGATCAGCTCCAGTCGC
AGGGCGGCAGTATGATCATGCTGGCGAAAGGCAACCGCAGCCAGCAGGTGACCGATGCCTGTAAGAAACATGGCGGCTTC
TACCTGGGCAGTATCGGCGGCCCGGCAGCCGTTCTGGCGCAGGGTAGTATCAAGCGCCTGGAGTGCGTGGAATATCCTGA
ACTGGGTATGGAGGCTATCTGGAAAATTGAAGTGGAGGATTTTCCGGCCTTCATTCTGGTGGATGATAAAGGCAACGATT
TCTTCCAGCAGATTCAGTCATCGCAGTGCGGCGCTGCGTTAAGTAATGTCGCCGCCCTGCGGGGCGGTAACATGATTCGC
TATTTTGCCGGAGAACGGCGCAAGCGCCTTATCCGGTCTACACCGTTGTGTTGTTATCGTTAA

Upstream 100 bases:

>100_bases
TTTTGTTAAAAAATTGTGTAGGATAGTGTTACTCGCTTTGAGTCGGGCAATGGAACACCTGCCCAGAGAATAACCATACC
GAGCGGTAAGTGAGAGCACA

Downstream 100 bases:

>100_bases
TACTCATAAGACCGCAAACATGTGAGCAATGTCGACACATGATCTCATCAACTTGAGTAAGGAGCAGGTAATGGTAACGG
TACGCCGCGAGAAAGATTCA

Product: fumarase A

Products: NA

Alternate protein names: Fumarase

Number of amino acids: Translated: 580; Mature: 579

Protein sequence:

>580_residues
MSNKPFFYQDPFPLKKDDTEYYLLTSEHVSVAEFEGQEILKVAPEALTLLARQAFHDASFMLRPAHQQQVADILRDPQAS
ENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAALDMYKEVNT
GTNLPAQIDLYSVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVDKMRTLGTAACPPYHIAFVIGGTSAEA
NLKTVKLASAKYYDALPTEGNEHGQAFRDIELEKELLLEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSA
DRNIKAKINRDGIWIEKLERNPGKYIPEALRQAGEGEAVRVDLNRPMSEILQQLSQYPVSTRLSLNGTIIVGRDIAHAKL
KERMDRGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQSQGGSMIMLAKGNRSQQVTDACKKHGGF
YLGSIGGPAAVLAQGSIKRLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQSSQCGAALSNVAALRGGNMIR
YFAGERRKRLIRSTPLCCYR

Sequences:

>Translated_580_residues
MSNKPFFYQDPFPLKKDDTEYYLLTSEHVSVAEFEGQEILKVAPEALTLLARQAFHDASFMLRPAHQQQVADILRDPQAS
ENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAALDMYKEVNT
GTNLPAQIDLYSVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVDKMRTLGTAACPPYHIAFVIGGTSAEA
NLKTVKLASAKYYDALPTEGNEHGQAFRDIELEKELLLEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSA
DRNIKAKINRDGIWIEKLERNPGKYIPEALRQAGEGEAVRVDLNRPMSEILQQLSQYPVSTRLSLNGTIIVGRDIAHAKL
KERMDRGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQSQGGSMIMLAKGNRSQQVTDACKKHGGF
YLGSIGGPAAVLAQGSIKRLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQSSQCGAALSNVAALRGGNMIR
YFAGERRKRLIRSTPLCCYR
>Mature_579_residues
SNKPFFYQDPFPLKKDDTEYYLLTSEHVSVAEFEGQEILKVAPEALTLLARQAFHDASFMLRPAHQQQVADILRDPQASE
NDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAALDMYKEVNTG
TNLPAQIDLYSVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVDKMRTLGTAACPPYHIAFVIGGTSAEAN
LKTVKLASAKYYDALPTEGNEHGQAFRDIELEKELLLEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSAD
RNIKAKINRDGIWIEKLERNPGKYIPEALRQAGEGEAVRVDLNRPMSEILQQLSQYPVSTRLSLNGTIIVGRDIAHAKLK
ERMDRGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQSQGGSMIMLAKGNRSQQVTDACKKHGGFY
LGSIGGPAAVLAQGSIKRLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQSSQCGAALSNVAALRGGNMIRY
FAGERRKRLIRSTPLCCYR

Specific function: It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family

Homologues:

Organism=Escherichia coli, GI1787897, Length=543, Percent_Identity=95.9484346224678, Blast_Score=1085, Evalue=0.0,
Organism=Escherichia coli, GI1790564, Length=543, Percent_Identity=89.134438305709, Blast_Score=1015, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=170, Percent_Identity=28.2352941176471, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): FUMA_SALTY (P40720)

Other databases:

- EMBL:   AE006468
- EMBL:   X57117
- RefSeq:   NP_460429.1
- ProteinModelPortal:   P40720
- SMR:   P40720
- PRIDE:   P40720
- GeneID:   1252986
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM1468
- NMPDR:   fig|99287.1.peg.1420
- HOGENOM:   HBG294102
- OMA:   FQQIQTS
- ProtClustDB:   PRK15390
- BioCyc:   STYP99287:STM1468-MONOMER
- BRENDA:   4.2.1.2
- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557
- Gene3D:   G3DSA:3.20.130.10
- PIRSF:   PIRSF001394
- TIGRFAMs:   TIGR00722
- TIGRFAMs:   TIGR00723

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C

EC number: =4.2.1.2

Molecular weight: Translated: 63825; Mature: 63694

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: ACT_SITE 397-397 BINDING 463-463

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKPFFYQDPFPLKKDDTEYYLLTSEHVSVAEFEGQEILKVAPEALTLLARQAFHDASF
CCCCCEEECCCCCCCCCCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCHH
MLRPAHQQQVADILRDPQASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQR
EECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCE
VWTGGGDEAALARGVYNTYIEDNLRYSQNAALDMYKEVNTGTNLPAQIDLYSVDGDEYKF
EEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCEEE
LCIAKGGGSANKTYLYQETKALLTPGKLKNYLVDKMRTLGTAACPPYHIAFVIGGTSAEA
EEEECCCCCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
NLKTVKLASAKYYDALPTEGNEHGQAFRDIELEKELLLEAQNLGLGAQFGGKYFAHDIRV
CEEEEEEECCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECEEE
IRLPRHGASCPVGMGVSCSADRNIKAKINRDGIWIEKLERNPGKYIPEALRQAGEGEAVR
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHCCCCCEEE
VDLNRPMSEILQQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDRGEGLPQYIKDHPIY
EECCCCHHHHHHHHHCCCCEEEEEECEEEEEECCHHHHHHHHHHHHCCCCHHHHHCCCEE
YAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQSQGGSMIMLAKGNRSQQVTDACKKHGGF
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQGSIKRLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQS
EEECCCCCCCEEECCCCCCEEECCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHC
SQCGAALSNVAALRGGNMIRYFAGERRKRLIRSTPLCCYR
CHHHHHHHHHHHHCCCCEEEEHHCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SNKPFFYQDPFPLKKDDTEYYLLTSEHVSVAEFEGQEILKVAPEALTLLARQAFHDASF
CCCCEEECCCCCCCCCCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCHH
MLRPAHQQQVADILRDPQASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQR
EECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCE
VWTGGGDEAALARGVYNTYIEDNLRYSQNAALDMYKEVNTGTNLPAQIDLYSVDGDEYKF
EEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCEEE
LCIAKGGGSANKTYLYQETKALLTPGKLKNYLVDKMRTLGTAACPPYHIAFVIGGTSAEA
EEEECCCCCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
NLKTVKLASAKYYDALPTEGNEHGQAFRDIELEKELLLEAQNLGLGAQFGGKYFAHDIRV
CEEEEEEECCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECEEE
IRLPRHGASCPVGMGVSCSADRNIKAKINRDGIWIEKLERNPGKYIPEALRQAGEGEAVR
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHCCCCCEEE
VDLNRPMSEILQQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDRGEGLPQYIKDHPIY
EECCCCHHHHHHHHHCCCCEEEEEECEEEEEECCHHHHHHHHHHHHCCCCHHHHHCCCEE
YAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQSQGGSMIMLAKGNRSQQVTDACKKHGGF
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQGSIKRLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQS
EEECCCCCCCEEECCCCCCEEECCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHC
SQCGAALSNVAALRGGNMIRYFAGERRKRLIRSTPLCCYR
CHHHHHHHHHHHHCCCCEEEEHHCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 1879695