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The map label for this gene is ssaB

Identifier: 16764743

GI number: 16764743

Start: 1479999

End: 1480400

Strand: Direct

Name: ssaB

Synonym: STM1393

Alternate gene names: 16764743

Gene position: 1479999-1480400 (Clockwise)

Preceding gene: 16764738

Following gene: 16764744

Centisome position: 30.47

GC content: 41.29

Gene sequence:

>402_bases
ATGTCTGAGGAGGGATTCATGCTGGCAGTTTTAAAAGGCATTCCATTAATTCAGGATATCAGGGCCGAAGGTAATAGCCG
ATCCTGGATAATGACTATTGATGGGCATCCTGCCAGAGGAGAAATTTTCTCAGAAGCATTTTCTATTTCTTTGTTCTTAA
ATGACCTGGAAAGCTTACCTAAGCCTTGTCTTGCCTATGTGACACTACTGCTTGCAGCACACCCGGACGTCCATGATTAT
GCTATACAGCTCACAGCGGATGGGGGATGGTTAAACGGTTATTATACCACAAGTAGTAGCTCTGAGCTTATTGCTATTGA
GATAGAAAAACACCTGGCTTTAACTTGCATTTTAAAAAATGTAATACGCAATCACCATAAACTTTATTCGGGTGGGGTAT
AA

Upstream 100 bases:

>100_bases
CAGCATCCTGATATTGTACAAGAAGAGTATAGTCGAAATAAATGTGAATCAGGCTTTTTACGGATGTGGTTGTGAGCGAA
TTTGATAGAAACTCCCATTT

Downstream 100 bases:

>100_bases
AATGGTAGTAAATAAACGTTTAATCTTAATTTTACTATTTATACTCAATACAGCAAAGAGTGATGAGTTATCATGGAAAG
GTAATGACTTCACCCTTTAT

Product: secreted effector protein

Products: NA

Alternate protein names: Secretion system apparatus protein B

Number of amino acids: Translated: 133; Mature: 132

Protein sequence:

>133_residues
MSEEGFMLAVLKGIPLIQDIRAEGNSRSWIMTIDGHPARGEIFSEAFSISLFLNDLESLPKPCLAYVTLLLAAHPDVHDY
AIQLTADGGWLNGYYTTSSSSELIAIEIEKHLALTCILKNVIRNHHKLYSGGV

Sequences:

>Translated_133_residues
MSEEGFMLAVLKGIPLIQDIRAEGNSRSWIMTIDGHPARGEIFSEAFSISLFLNDLESLPKPCLAYVTLLLAAHPDVHDY
AIQLTADGGWLNGYYTTSSSSELIAIEIEKHLALTCILKNVIRNHHKLYSGGV
>Mature_132_residues
SEEGFMLAVLKGIPLIQDIRAEGNSRSWIMTIDGHPARGEIFSEAFSISLFLNDLESLPKPCLAYVTLLLAAHPDVHDYA
IQLTADGGWLNGYYTTSSSSELIAIEIEKHLALTCILKNVIRNHHKLYSGGV

Specific function: Virulence protein that plays a central role in mammalian macrophage infection, by inhibiting phagosome-lysosome fusion and cellular trafficking. May act by disrupting the function of the mammalian HOOK3 protein, a protein involved in the cellular traffic

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Cytoplasm. Note=Translocated into the cytosol of macrophages via an unique type III secretion system that functions intracellularly to translocate proteins across the phagosomal membrane. Cytoplasmic in infected macrophage

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPIC_SALTY (P74863)

Other databases:

- EMBL:   U51927
- EMBL:   AE006468
- RefSeq:   NP_460358.1
- GeneID:   1252911
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM1393
- HOGENOM:   HBG673238
- OMA:   IQDIRAE
- ProtClustDB:   PRK15368
- BioCyc:   STYP99287:STM1393-MONOMER
- GO:   GO:0005737
- GO:   GO:0009405

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 14656; Mature: 14525

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEGFMLAVLKGIPLIQDIRAEGNSRSWIMTIDGHPARGEIFSEAFSISLFLNDLESLP
CCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
KPCLAYVTLLLAAHPDVHDYAIQLTADGGWLNGYYTTSSSSELIAIEIEKHLALTCILKN
HHHHHHHHHHHHCCCCCCCEEEEEEECCCEECCEEECCCCCCEEEEEECHHHHHHHHHHH
VIRNHHKLYSGGV
HHHHHHHHHCCCC
>Mature Secondary Structure 
SEEGFMLAVLKGIPLIQDIRAEGNSRSWIMTIDGHPARGEIFSEAFSISLFLNDLESLP
CCCCEEEEEECCCCHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
KPCLAYVTLLLAAHPDVHDYAIQLTADGGWLNGYYTTSSSSELIAIEIEKHLALTCILKN
HHHHHHHHHHHHCCCCCCCEEEEEEECCCEECCEEECCCCCCEEEEEECHHHHHHHHHHH
VIRNHHKLYSGGV
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8755556; 11677609