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The map label for this gene is celF [H]

Identifier: 16764667

GI number: 16764667

Start: 1394944

End: 1396299

Strand: Direct

Name: celF [H]

Synonym: STM1316

Alternate gene names: 16764667

Gene position: 1394944-1396299 (Clockwise)

Preceding gene: 16764666

Following gene: 16764668

Centisome position: 28.72

GC content: 48.08

Gene sequence:

>1356_bases
ATGAGCCAGAAATTAAAAGTCGTCACTATTGGTGGCGGGAGCAGCTACACCCCTGAATTACTTGAAGGCTTTATTAAGCG
CTACCATGAATTACCTGTCACCGAATTATGGCTGGTTGACGTTGAAGACGGGAAAGAGAAGCTGGGCATTATTTATGATC
TCTGCCAGCGAATGATTGATAAAGCAGGCGTTCCGCTAAAATTGTATAAAACGCTGGATCGCCGGGAAGCGCTGAAAGGC
GCTAATTTTGTCACTACCCAGCTACGCGTTGGTCAACTCAAAGCCCGTGAGCTGGACGAGCGTATCCCGCTTAGCCACGG
CTATCTGGGGCAAGAAACCAACGGCGCTGGCGGTTTATTTAAAGGGTTGCGTACCATTCCGGTTATTTTTGACATCATTA
AGGATGTTGAAGAATTATGTCCGAATGCGTGGGTCATTAACTTTACTAACCCGGCGGGGATGGTGACGGAAGCGGTTTAT
CGTCATACCAACTTTAAAAAATTCATCGGCGTATGTAATATTCCTGTCGGCATGAAAATGTTTATTCATGACGTGCTGGC
GCTGAATGAGAATGACGATCTTTCCATTGACCTGTTTGGTCTAAACCATATGGTCTTTATTAAAGATGTGCTGGTGAATG
GCACCTCACGGTTCGCAGAATTACTGGATGGCGTGGCGTCCGGTCAGTTGAAAGCGTCAACCGTAAAAAATATCTTTGAT
CTGCCGTTTAGTGAAGGATTGATTCGCTCGCTGAACATGCTGCCGTGCTCTTATTTGTTGTATTACTTCAAGCAAAAAGA
GATGTTGGCGATTGAAATGGGCGAATATTACAAAGGCGGCGCGCGCGCTCAGGTCGTACAGAAAGTGGAGAAACAACTCT
TCGACTTGTACAAAAATCCTGAGCTAAACGTGAAGCCGAAAGAGCTTGAGCAACGCGGCGGCGCTTATTATTCCGATGCC
GCTTGTGAAGTCATTAACGCTATTTATAATGACAAGCAGACTGAGCATTACGTTAATATTCCACATCATGGGCATGTCGA
GAATATCCCGGCGGACTGGGCGGTGGAAATGACCTGCATTCTGGGACGCAATGGCGCGACGCCGCACCCGCGTATCACCC
GTTTTGACGAAAAAGTGCTGGGGCTTATCCACACTATTAAAGGATTTGAGGTCGCGGCCAGCAATGCGGCGCTGAGCGGA
AACTTTAATGATGTTCTGCTGGCGCTTAACCTGAGTCCGCTGGTGCATTCCGACCGCGACGCAGAAGTCCTGGCGCGTGA
ACTCATTCTGGCGCATGAAAAATGGCTGCCTAATTTTGCCGCTTGCATCGAAGCGCTTAAAGGTAAGCACCACTGA

Upstream 100 bases:

>100_bases
CATTTCACGCTTGCCCCAATAGCGCGGGGAACGTCACGCTATTTCTTACGAGCGGGCATAGCGTAATACATATCAGTGTT
ATACACTGAAGGGAGATATT

Downstream 100 bases:

>100_bases
CAGAGGTAGGCTATGGAACGCGTGTTGATTGTGAATGCCGATGATTTTGGCCTGAGTAAAGGGCAAAATTACGGCATTGT
TGAAGCATATCGCAACGGCG

Product: phospho-beta-glucosidase/cellobiose-6-phosphate hydrolase

Products: NA

Alternate protein names: Cellobiose-6-phosphate hydrolase; Phospho-chitobiase [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVTELWLVDVEDGKEKLGIIYDLCQRMIDKAGVPLKLYKTLDRREALKG
ANFVTTQLRVGQLKARELDERIPLSHGYLGQETNGAGGLFKGLRTIPVIFDIIKDVEELCPNAWVINFTNPAGMVTEAVY
RHTNFKKFIGVCNIPVGMKMFIHDVLALNENDDLSIDLFGLNHMVFIKDVLVNGTSRFAELLDGVASGQLKASTVKNIFD
LPFSEGLIRSLNMLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFDLYKNPELNVKPKELEQRGGAYYSDA
ACEVINAIYNDKQTEHYVNIPHHGHVENIPADWAVEMTCILGRNGATPHPRITRFDEKVLGLIHTIKGFEVAASNAALSG
NFNDVLLALNLSPLVHSDRDAEVLARELILAHEKWLPNFAACIEALKGKHH

Sequences:

>Translated_451_residues
MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVTELWLVDVEDGKEKLGIIYDLCQRMIDKAGVPLKLYKTLDRREALKG
ANFVTTQLRVGQLKARELDERIPLSHGYLGQETNGAGGLFKGLRTIPVIFDIIKDVEELCPNAWVINFTNPAGMVTEAVY
RHTNFKKFIGVCNIPVGMKMFIHDVLALNENDDLSIDLFGLNHMVFIKDVLVNGTSRFAELLDGVASGQLKASTVKNIFD
LPFSEGLIRSLNMLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFDLYKNPELNVKPKELEQRGGAYYSDA
ACEVINAIYNDKQTEHYVNIPHHGHVENIPADWAVEMTCILGRNGATPHPRITRFDEKVLGLIHTIKGFEVAASNAALSG
NFNDVLLALNLSPLVHSDRDAEVLARELILAHEKWLPNFAACIEALKGKHH
>Mature_450_residues
SQKLKVVTIGGGSSYTPELLEGFIKRYHELPVTELWLVDVEDGKEKLGIIYDLCQRMIDKAGVPLKLYKTLDRREALKGA
NFVTTQLRVGQLKARELDERIPLSHGYLGQETNGAGGLFKGLRTIPVIFDIIKDVEELCPNAWVINFTNPAGMVTEAVYR
HTNFKKFIGVCNIPVGMKMFIHDVLALNENDDLSIDLFGLNHMVFIKDVLVNGTSRFAELLDGVASGQLKASTVKNIFDL
PFSEGLIRSLNMLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFDLYKNPELNVKPKELEQRGGAYYSDAA
CEVINAIYNDKQTEHYVNIPHHGHVENIPADWAVEMTCILGRNGATPHPRITRFDEKVLGLIHTIKGFEVAASNAALSGN
FNDVLLALNLSPLVHSDRDAEVLARELILAHEKWLPNFAACIEALKGKHH

Specific function: Hydrolyzes a wide variety of P-beta-glucosides including cellobiose-6P, salicin-6P, arbutin-6P, gentiobiose-6P, methyl- beta-glucoside-6P and p-nitrophenyl-beta-D-glucopyranoside-6P. Is also able to hydrolyze phospho-N,N'-diacetylchitobiose [H]

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=450, Percent_Identity=90.4444444444444, Blast_Score=854, Evalue=0.0,
Organism=Escherichia coli, GI1790560, Length=462, Percent_Identity=25.5411255411255, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 50539; Mature: 50408

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVTELWLVDVEDGKEKLGIIYDLCQRMID
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
KAGVPLKLYKTLDRREALKGANFVTTQLRVGQLKARELDERIPLSHGYLGQETNGAGGLF
HCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
KGLRTIPVIFDIIKDVEELCPNAWVINFTNPAGMVTEAVYRHTNFKKFIGVCNIPVGMKM
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHCCCCCHHHH
FIHDVLALNENDDLSIDLFGLNHMVFIKDVLVNGTSRFAELLDGVASGQLKASTVKNIFD
HHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHC
LPFSEGLIRSLNMLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFDLYKNP
CCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHCCC
ELNVKPKELEQRGGAYYSDAACEVINAIYNDKQTEHYVNIPHHGHVENIPADWAVEMTCI
CCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHEEEEEE
LGRNGATPHPRITRFDEKVLGLIHTIKGFEVAASNAALSGNFNDVLLALNLSPLVHSDRD
ECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCEECCCCCEEEEEEECCHHHCCCCC
AEVLARELILAHEKWLPNFAACIEALKGKHH
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
SQKLKVVTIGGGSSYTPELLEGFIKRYHELPVTELWLVDVEDGKEKLGIIYDLCQRMID
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
KAGVPLKLYKTLDRREALKGANFVTTQLRVGQLKARELDERIPLSHGYLGQETNGAGGLF
HCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
KGLRTIPVIFDIIKDVEELCPNAWVINFTNPAGMVTEAVYRHTNFKKFIGVCNIPVGMKM
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHCCCCCHHHH
FIHDVLALNENDDLSIDLFGLNHMVFIKDVLVNGTSRFAELLDGVASGQLKASTVKNIFD
HHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHC
LPFSEGLIRSLNMLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFDLYKNP
CCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHCCC
ELNVKPKELEQRGGAYYSDAACEVINAIYNDKQTEHYVNIPHHGHVENIPADWAVEMTCI
CCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHEEEEEE
LGRNGATPHPRITRFDEKVLGLIHTIKGFEVAASNAALSGNFNDVLLALNLSPLVHSDRD
ECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCEECCCCCEEEEEEECCHHHCCCCC
AEVLARELILAHEKWLPNFAACIEALKGKHH
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2179047; 9097039; 9278503; 8121401; 10572139; 9405618; 10913117 [H]