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The map label for this gene is sifA

Identifier: 16764579

GI number: 16764579

Start: 1309844

End: 1310854

Strand: Reverse

Name: sifA

Synonym: STM1224

Alternate gene names: 16764579

Gene position: 1310854-1309844 (Counterclockwise)

Preceding gene: 16764580

Following gene: 16764578

Centisome position: 26.99

GC content: 41.15

Gene sequence:

>1011_bases
ATGCCGATTACTATAGGGAATGGTTTTTTAAAAAGTGAAATCCTTACCAACTCCCCAAGGAATACGAAAGAAGCATGGTG
GAAAGTTTTATGGGAAAAAATTAAAGACTTCTTTTTTTCTACTGGCAAAGCAAAAGCGGACCGTTGTCTACATGAGATGT
TGTTTGCCGAACGCGCCCCCACACGAGAGCGGCTTACAGAGATTTTTTTTGAGTTGAAAGAGTTAGCCTGCGCATCGCAA
AGAGATAGATTTCAGGTTCATAATCCTCATGAAAATGATGCCACCATTATTCTTCGCATCATGGATCAAAACGAAGAGAA
CGAATTGTTACGTATCACTCAAAATACCGATACCTTTAGCTGTGAAGTCATGGGGAATCTTTATTTTTTAATGAAAGATC
GCCCGGATATTTTAAAATCGCATCCACAAATGACGGCCATGATTAAGAGAAGATATAGCGAAATCGTAGACTACCCCCTC
CCTTCGACATTATGTCTCAATCCTGCTGGCGCGCCGATATTATCGGTTCCATTAGACAACATAGAGGGGTATTTATATAC
TGAATTGAGAAAAGGACATTTAGATGGGTGGAAAGCGCAAGAAAAGGCAACCTACCTGGCAGCGAAAATTCAGTCTGGGA
TTGAAAAGACAACGCGCATTTTACACCATGCGAATATATCCGAAAGTACTCAGCAAAACGCATTTTTAGAAACAATGGCG
ATGTGTGGATTAAAACAGCTTGAAATACCACCACCGCATACCCACATACCTATTGAAAAAATGGTAAAAGAGGTTTTACT
AGCGGATAAGACGTTTCAGGCGTTCCTCGTAACGGATCCCAGCACCAGCCAAAGTATGTTAGCTGAGATAGTCGAAGCCA
TCTCTGATCAGGTTTTTCACGCCATTTTTAGAATAGACCCCCAGGCTATACAAAAAATGGCGGAAGAACAGTTAACCACG
CTACACGTTCGCTCAGAACAACAAAGCGGCTGTTTATGTTGTTTTTTATAA

Upstream 100 bases:

>100_bases
TTAAAAATTATATTACATCCGATGCGCCCGCAGTTGAGATAAAAAGGGTCGATTTAATCAATTATGTAGTCATTTTTACT
CCAGTATAAGTGAGATTAAT

Downstream 100 bases:

>100_bases
AATCAGACGACGCTTTCTCAGACGTCACGTTCAGGGTTTACTCACGTACTTCCTGTTTGCCTACTGAGTAACCTCTTGCC
AGGCACAATAATGCCGCATA

Product: secreted effector protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MPITIGNGFLKSEILTNSPRNTKEAWWKVLWEKIKDFFFSTGKAKADRCLHEMLFAERAPTRERLTEIFFELKELACASQ
RDRFQVHNPHENDATIILRIMDQNEENELLRITQNTDTFSCEVMGNLYFLMKDRPDILKSHPQMTAMIKRRYSEIVDYPL
PSTLCLNPAGAPILSVPLDNIEGYLYTELRKGHLDGWKAQEKATYLAAKIQSGIEKTTRILHHANISESTQQNAFLETMA
MCGLKQLEIPPPHTHIPIEKMVKEVLLADKTFQAFLVTDPSTSQSMLAEIVEAISDQVFHAIFRIDPQAIQKMAEEQLTT
LHVRSEQQSGCLCCFL

Sequences:

>Translated_336_residues
MPITIGNGFLKSEILTNSPRNTKEAWWKVLWEKIKDFFFSTGKAKADRCLHEMLFAERAPTRERLTEIFFELKELACASQ
RDRFQVHNPHENDATIILRIMDQNEENELLRITQNTDTFSCEVMGNLYFLMKDRPDILKSHPQMTAMIKRRYSEIVDYPL
PSTLCLNPAGAPILSVPLDNIEGYLYTELRKGHLDGWKAQEKATYLAAKIQSGIEKTTRILHHANISESTQQNAFLETMA
MCGLKQLEIPPPHTHIPIEKMVKEVLLADKTFQAFLVTDPSTSQSMLAEIVEAISDQVFHAIFRIDPQAIQKMAEEQLTT
LHVRSEQQSGCLCCFL
>Mature_335_residues
PITIGNGFLKSEILTNSPRNTKEAWWKVLWEKIKDFFFSTGKAKADRCLHEMLFAERAPTRERLTEIFFELKELACASQR
DRFQVHNPHENDATIILRIMDQNEENELLRITQNTDTFSCEVMGNLYFLMKDRPDILKSHPQMTAMIKRRYSEIVDYPLP
STLCLNPAGAPILSVPLDNIEGYLYTELRKGHLDGWKAQEKATYLAAKIQSGIEKTTRILHHANISESTQQNAFLETMAM
CGLKQLEIPPPHTHIPIEKMVKEVLLADKTFQAFLVTDPSTSQSMLAEIVEAISDQVFHAIFRIDPQAIQKMAEEQLTTL
HVRSEQQSGCLCCFL

Specific function: Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is required for endosomal tubulation and formation of Salmonella- induced filaments (Sifs), which are filamentous structures containing l

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Host cytoplasm. Host cell membrane. Note=Secreted via type III secretion system 2 (SPI-2 TTSS), and delivered into the host cytoplasm. It associates with membranes and colocalizes with microtubules along the length of Sifs in infected cells

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Sif family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SIFA_SALTY (Q56061)

Other databases:

- EMBL:   U51867
- EMBL:   AE006468
- PIR:   S71033
- RefSeq:   NP_460194.1
- PDB:   3CXB
- PDB:   3HW2
- PDBsum:   3CXB
- PDBsum:   3HW2
- DIP:   DIP-46411N
- PRIDE:   Q56061
- GeneID:   1252742
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM1224
- NMPDR:   fig|99287.1.peg.1185
- HOGENOM:   HBG671339
- OMA:   AFLETMT
- ProtClustDB:   PRK09498
- BioCyc:   STYP99287:STM1224-MONOMER
- GO:   GO:0016020
- GO:   GO:0009405
- InterPro:   IPR010637

Pfam domain/function: PF06767 Sif

EC number: NA

Molecular weight: Translated: 38499; Mature: 38368

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPITIGNGFLKSEILTNSPRNTKEAWWKVLWEKIKDFFFSTGKAKADRCLHEMLFAERAP
CCEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
TRERLTEIFFELKELACASQRDRFQVHNPHENDATIILRIMDQNEENELLRITQNTDTFS
HHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEEEEECCCCCCCEEEEECCCCCEE
CEVMGNLYFLMKDRPDILKSHPQMTAMIKRRYSEIVDYPLPSTLCLNPAGAPILSVPLDN
EEEECCEEEEECCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEECCHHC
IEGYLYTELRKGHLDGWKAQEKATYLAAKIQSGIEKTTRILHHANISESTQQNAFLETMA
CCCCEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
MCGLKQLEIPPPHTHIPIEKMVKEVLLADKTFQAFLVTDPSTSQSMLAEIVEAISDQVFH
HCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHH
AIFRIDPQAIQKMAEEQLTTLHVRSEQQSGCLCCFL
HHHHCCHHHHHHHHHHHHHEEEEECCCCCCCEEEEC
>Mature Secondary Structure 
PITIGNGFLKSEILTNSPRNTKEAWWKVLWEKIKDFFFSTGKAKADRCLHEMLFAERAP
CEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
TRERLTEIFFELKELACASQRDRFQVHNPHENDATIILRIMDQNEENELLRITQNTDTFS
HHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEEEEECCCCCCCEEEEECCCCCEE
CEVMGNLYFLMKDRPDILKSHPQMTAMIKRRYSEIVDYPLPSTLCLNPAGAPILSVPLDN
EEEECCEEEEECCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEECCHHC
IEGYLYTELRKGHLDGWKAQEKATYLAAKIQSGIEKTTRILHHANISESTQQNAFLETMA
CCCCEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
MCGLKQLEIPPPHTHIPIEKMVKEVLLADKTFQAFLVTDPSTSQSMLAEIVEAISDQVFH
HCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHH
AIFRIDPQAIQKMAEEQLTTLHVRSEQQSGCLCCFL
HHHHCCHHHHHHHHHHHHHEEEEECCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8861213; 11677609; 10861017