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The map label for this gene is flgJ

Identifier: 16764538

GI number: 16764538

Start: 1264453

End: 1265403

Strand: Direct

Name: flgJ

Synonym: STM1182

Alternate gene names: 16764538

Gene position: 1264453-1265403 (Clockwise)

Preceding gene: 16764537

Following gene: 16764539

Centisome position: 26.03

GC content: 56.26

Gene sequence:

>951_bases
ATGATCGGAGACGGTAAATTGCTGGCCAGCGCGGCCTGGGATGCGCAATCTCTGAACGAACTGAAAGCGAAAGCGGGCCA
GGACCCGGCGGCGAATATCCGTCCTGTGGCCCGTCAGGTGGAAGGGATGTTTGTGCAGATGATGCTGAAAAGTATGCGCG
AGGCTTTACCCAAAGATGGTTTATTCAGCAGCGATCAGACGCGTCTGTATACCAGCATGTATGACCAGCAGATCGCCCAG
CAGATGACCGCCGGTAAGGGATTGGGGCTGGCGGATATGATGGTTAAACAGATGACCAGCGGGCAGACGATGCCTGCGGA
TGACGCGCCGCAAGTACCGCTTAAATTCTCCCTGGAGACGGTAAACAGCTATCAAAATCAGGCGCTGACCCAACTGGTGC
GCAAAGCCATACCGAAAACGCCGGACAGCAGCGATGCGCCGCTCTCCGGCGACAGTAAAGACTTTCTGGCCCGGCTTTCG
CTCCCTGCGAGGCTGGCCAGCGAACAAAGCGGGGTGCCGCATCATCTGATTCTGGCGCAGGCGGCACTGGAGTCCGGCTG
GGGGCAGCGGCAAATCCTGCGGGAGAATGGCGAACCCAGCTATAACGTATTTGGCGTGAAAGCGACCGCCAGTTGGAAAG
GGCCGGTGACGGAGATCACCACCACTGAATACGAAAATGGCGAAGCGAAAAAAGTGAAAGCGAAATTCCGCGTCTATAGC
TCGTATCTGGAGGCATTATCGGATTATGTCGCGCTGTTAACGCGTAACCCACGCTACGCTGCCGTGACCACTGCCGCCAC
GGCAGAGCAGGGCGCAGTGGCTCTGCAAAACGCCGGATACGCCACTGACCCGAATTACGCGCGTAAATTGACCAGCATGA
TTCAGCAGTTGAAAGCGATGAGTGAAAAGGTCAGCAAAACCTACAGCGCGAATCTCGACAATCTCTTTTAA

Upstream 100 bases:

>100_bases
GTGCGCTGAATGCGCTTGGCGCGACGCCGATGGATCTGATGTCGATTTTGCAGTCCATGCAGAGCGCGGGCTGTCTACGC
GCCAAACTGGAAATCATCTG

Downstream 100 bases:

>100_bases
ATTGCTCAAGTCCACGTAGTCGCTGCCGATAACAACGAGTATTGAAGGATTAAAAGGAACCATCATGTCCAGCTTGATTA
ATCACGCCATGAGCGGACTT

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MIGDGKLLASAAWDAQSLNELKAKAGQDPAANIRPVARQVEGMFVQMMLKSMREALPKDGLFSSDQTRLYTSMYDQQIAQ
QMTAGKGLGLADMMVKQMTSGQTMPADDAPQVPLKFSLETVNSYQNQALTQLVRKAIPKTPDSSDAPLSGDSKDFLARLS
LPARLASEQSGVPHHLILAQAALESGWGQRQILRENGEPSYNVFGVKATASWKGPVTEITTTEYENGEAKKVKAKFRVYS
SYLEALSDYVALLTRNPRYAAVTTAATAEQGAVALQNAGYATDPNYARKLTSMIQQLKAMSEKVSKTYSANLDNLF

Sequences:

>Translated_316_residues
MIGDGKLLASAAWDAQSLNELKAKAGQDPAANIRPVARQVEGMFVQMMLKSMREALPKDGLFSSDQTRLYTSMYDQQIAQ
QMTAGKGLGLADMMVKQMTSGQTMPADDAPQVPLKFSLETVNSYQNQALTQLVRKAIPKTPDSSDAPLSGDSKDFLARLS
LPARLASEQSGVPHHLILAQAALESGWGQRQILRENGEPSYNVFGVKATASWKGPVTEITTTEYENGEAKKVKAKFRVYS
SYLEALSDYVALLTRNPRYAAVTTAATAEQGAVALQNAGYATDPNYARKLTSMIQQLKAMSEKVSKTYSANLDNLF
>Mature_316_residues
MIGDGKLLASAAWDAQSLNELKAKAGQDPAANIRPVARQVEGMFVQMMLKSMREALPKDGLFSSDQTRLYTSMYDQQIAQ
QMTAGKGLGLADMMVKQMTSGQTMPADDAPQVPLKFSLETVNSYQNQALTQLVRKAIPKTPDSSDAPLSGDSKDFLARLS
LPARLASEQSGVPHHLILAQAALESGWGQRQILRENGEPSYNVFGVKATASWKGPVTEITTTEYENGEAKKVKAKFRVYS
SYLEALSDYVALLTRNPRYAAVTTAATAEQGAVALQNAGYATDPNYARKLTSMIQQLKAMSEKVSKTYSANLDNLF

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space

COG id: COG3951

COG function: function code MNO; Rod binding protein

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family

Homologues:

Organism=Escherichia coli, GI1787321, Length=316, Percent_Identity=83.2278481012658, Blast_Score=540, Evalue=1e-155,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): FLGJ_SALTY (P15931)

Other databases:

- EMBL:   M24466
- EMBL:   AE006468
- EMBL:   X51738
- PIR:   D32887
- RefSeq:   NP_460153.1
- GeneID:   1252700
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM1182
- NMPDR:   fig|99287.1.peg.1144
- HOGENOM:   HBG336388
- OMA:   SMRDANA
- ProtClustDB:   PRK05684
- BioCyc:   STYP99287:STM1182-MONOMER
- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901
- PRINTS:   PR01002
- SMART:   SM00047
- TIGRFAMs:   TIGR02541

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding

EC number: 3.2.1.-

Molecular weight: Translated: 34385; Mature: 34385

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: ACT_SITE 223-223 ACT_SITE 248-248

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGDGKLLASAAWDAQSLNELKAKAGQDPAANIRPVARQVEGMFVQMMLKSMREALPKDG
CCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LFSSDQTRLYTSMYDQQIAQQMTAGKGLGLADMMVKQMTSGQTMPADDAPQVPLKFSLET
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHH
VNSYQNQALTQLVRKAIPKTPDSSDAPLSGDSKDFLARLSLPARLASEQSGVPHHLILAQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHCCCCCHHHHHHH
AALESGWGQRQILRENGEPSYNVFGVKATASWKGPVTEITTTEYENGEAKKVKAKFRVYS
HHHHCCCCHHHHHHCCCCCCEEEEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHH
SYLEALSDYVALLTRNPRYAAVTTAATAEQGAVALQNAGYATDPNYARKLTSMIQQLKAM
HHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHH
SEKVSKTYSANLDNLF
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIGDGKLLASAAWDAQSLNELKAKAGQDPAANIRPVARQVEGMFVQMMLKSMREALPKDG
CCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LFSSDQTRLYTSMYDQQIAQQMTAGKGLGLADMMVKQMTSGQTMPADDAPQVPLKFSLET
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHH
VNSYQNQALTQLVRKAIPKTPDSSDAPLSGDSKDFLARLSLPARLASEQSGVPHHLILAQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHCCCCCHHHHHHH
AALESGWGQRQILRENGEPSYNVFGVKATASWKGPVTEITTTEYENGEAKKVKAKFRVYS
HHHHCCCCHHHHHHCCCCCCEEEEEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHH
SYLEALSDYVALLTRNPRYAAVTTAATAEQGAVALQNAGYATDPNYARKLTSMIQQLKAM
HHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHH
SEKVSKTYSANLDNLF
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2544561; 11677609; 2193164; 10049388