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The map label for this gene is yccW

Identifier: 16764439

GI number: 16764439

Start: 1170746

End: 1171957

Strand: Reverse

Name: yccW

Synonym: STM1080

Alternate gene names: 16764439

Gene position: 1171957-1170746 (Counterclockwise)

Preceding gene: 16764443

Following gene: 16764438

Centisome position: 24.13

GC content: 54.21

Gene sequence:

>1212_bases
ATGACTGAATCTACATTTCCGCAATATCCCCGTTTAGTCCTCAGCAAAGGGCGAGAAAAATCTTTACTCCGCCGCCACCC
ATGGGTCTTTTCCGGCGCCGTATCCCGTCTGGAAGGCAAAGCCAACCTCGGTGAAACTATCGATATCGTCGACCATCAGG
GAAAGTGGTTAGCACGCGGCGCCTGGTCACCAGCCTCCCAGATCCGCGCGCGCGTCTGGACATTTGATAAAGCAGAATCC
ATTGATATTGCGTTTTTCACCCGCCGCCTGCGCCAGGCGCAGCAGTGGCGCGACTGGCTGGCGAAAAAAGACGGCCTGGA
TAGCTATCGTCTGATCGCCGGTGAGTCCGATGGCCTGCCTGGCGTCACTATCGATCGTTTTGGTCATTTTTTGGTGCTGC
AACTGCTCAGCGCCGGGGCCGAATATCAACGCGCCGCATTAATTAGCGCGCTGCAAACATGCTATCCGGATTGCGCTATT
TACGATCGCAGCGACGTCGCCGTGCGGAAAAAAGAAGGGATGGCGCTGACGCAAGGTCCGGTCACTGGCGAACTGCCGCC
TGCGCTTTTGCCAATTGAAGAACACGGTATGAAATTGCTGGTCGATATCCAGGGCGGCCACAAAACCGGTTATTATCTTG
ATCAGCGCGACAGTCGTCTGGCGACGCGCCGCTACGTGGAAAATCAGCGGGTACTGAACTGCTTCTCTTATACCGGCGGT
TTTGCCGTGTCGGCGTTAATGGGCGGTTGTCGCCAGGTTGTCAGCGTGGATACCTCACAGGATGCGCTGGATATCGCCAG
GCAAAACGTTGAACTGAACCAACTGGACTTGAGCAAAGCCGAATTCGTGCGCGACGACGTGTTTAAGTTGCTGCGCGCTT
ACCGTGAACACGGCGAAAAATTCGACGTCATCATCATGGACCCGCCCAAATTCGTTGAAAATAAAAGCCAGTTAATGGGC
GCCTGCCGGGGCTATAAAGACATTAACATGTTAGCGATTCAACTGCTCAATCCGGGCGGCATACTGCTGACATTCTCTTG
CTCCGGACTGATGACCAGCGATTTATTTCAGAAAATCATTGCCGATGCCGCAATAGATGCCGGTCGTGATGTACAATTTA
TAGAGCAGTTCCGTCAGGCCGCCGATCACCCGGTGATCGCCACCTACCCGGAAGGGCTGTATCTGAAAGGGTTTGCCTGT
CGCGTCATGTAA

Upstream 100 bases:

>100_bases
CCCCTCTCATCTGTGCTACCCTACGCGCCATTTCGTTTTCACGGTCCCAGGCTGGAACTATCATCCGGCTCGCGTCACTG
TTTGTGAGAGGTTACTCCGC

Downstream 100 bases:

>100_bases
CTTGAAAAGTGGAATAGTATCCTCATATAAAGGGTATCTATTTCCCGGGAGGTGACTATGATAGCCAGCAAATTCGGTAT
CGGCCAACAGGTCCGCCATT

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTESTFPQYPRLVLSKGREKSLLRRHPWVFSGAVSRLEGKANLGETIDIVDHQGKWLARGAWSPASQIRARVWTFDKAES
IDIAFFTRRLRQAQQWRDWLAKKDGLDSYRLIAGESDGLPGVTIDRFGHFLVLQLLSAGAEYQRAALISALQTCYPDCAI
YDRSDVAVRKKEGMALTQGPVTGELPPALLPIEEHGMKLLVDIQGGHKTGYYLDQRDSRLATRRYVENQRVLNCFSYTGG
FAVSALMGGCRQVVSVDTSQDALDIARQNVELNQLDLSKAEFVRDDVFKLLRAYREHGEKFDVIIMDPPKFVENKSQLMG
ACRGYKDINMLAIQLLNPGGILLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFAC
RVM

Sequences:

>Translated_403_residues
MTESTFPQYPRLVLSKGREKSLLRRHPWVFSGAVSRLEGKANLGETIDIVDHQGKWLARGAWSPASQIRARVWTFDKAES
IDIAFFTRRLRQAQQWRDWLAKKDGLDSYRLIAGESDGLPGVTIDRFGHFLVLQLLSAGAEYQRAALISALQTCYPDCAI
YDRSDVAVRKKEGMALTQGPVTGELPPALLPIEEHGMKLLVDIQGGHKTGYYLDQRDSRLATRRYVENQRVLNCFSYTGG
FAVSALMGGCRQVVSVDTSQDALDIARQNVELNQLDLSKAEFVRDDVFKLLRAYREHGEKFDVIIMDPPKFVENKSQLMG
ACRGYKDINMLAIQLLNPGGILLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFAC
RVM
>Mature_402_residues
TESTFPQYPRLVLSKGREKSLLRRHPWVFSGAVSRLEGKANLGETIDIVDHQGKWLARGAWSPASQIRARVWTFDKAESI
DIAFFTRRLRQAQQWRDWLAKKDGLDSYRLIAGESDGLPGVTIDRFGHFLVLQLLSAGAEYQRAALISALQTCYPDCAIY
DRSDVAVRKKEGMALTQGPVTGELPPALLPIEEHGMKLLVDIQGGHKTGYYLDQRDSRLATRRYVENQRVLNCFSYTGGF
AVSALMGGCRQVVSVDTSQDALDIARQNVELNQLDLSKAEFVRDDVFKLLRAYREHGEKFDVIIMDPPKFVENKSQLMGA
CRGYKDINMLAIQLLNPGGILLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFACR
VM

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Escherichia coli, GI145693111, Length=393, Percent_Identity=93.1297709923664, Blast_Score=763, Evalue=0.0,
Organism=Escherichia coli, GI1787181, Length=244, Percent_Identity=30.327868852459, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMI_SALA4 (B5F1W3)

Other databases:

- EMBL:   CP001138
- RefSeq:   YP_002145949.1
- ProteinModelPortal:   B5F1W3
- SMR:   B5F1W3
- PRIDE:   B5F1W3
- GeneID:   6795450
- GenomeReviews:   CP001138_GR
- KEGG:   sea:SeAg_B1038
- HOGENOM:   HBG411897
- OMA:   RAAYSPH
- ProtClustDB:   PRK15128
- GO:   GO:0005737
- HAMAP:   MF_01857
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614
- SMART:   SM00359

Pfam domain/function: PF10672 Methyltrans_SAM; SSF88697 PUA-like

EC number: =2.1.1.191

Molecular weight: Translated: 45176; Mature: 45045

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESTFPQYPRLVLSKGREKSLLRRHPWVFSGAVSRLEGKANLGETIDIVDHQGKWLARG
CCCCCCCCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEEECCCCEEECC
AWSPASQIRARVWTFDKAESIDIAFFTRRLRQAQQWRDWLAKKDGLDSYRLIAGESDGLP
CCCCHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
GVTIDRFGHFLVLQLLSAGAEYQRAALISALQTCYPDCAIYDRSDVAVRKKEGMALTQGP
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCEEECCCCCEEECCC
VTGELPPALLPIEEHGMKLLVDIQGGHKTGYYLDQRDSRLATRRYVENQRVLNCFSYTGG
CCCCCCCCCCCHHHCCCEEEEEECCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCH
FAVSALMGGCRQVVSVDTSQDALDIARQNVELNQLDLSKAEFVRDDVFKLLRAYREHGEK
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCE
FDVIIMDPPKFVENKSQLMGACRGYKDINMLAIQLLNPGGILLTFSCSGLMTSDLFQKII
EEEEEECCCHHHCCHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHH
ADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFACRVM
HHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCEEECCEEEECC
>Mature Secondary Structure 
TESTFPQYPRLVLSKGREKSLLRRHPWVFSGAVSRLEGKANLGETIDIVDHQGKWLARG
CCCCCCCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEEECCCCEEECC
AWSPASQIRARVWTFDKAESIDIAFFTRRLRQAQQWRDWLAKKDGLDSYRLIAGESDGLP
CCCCHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
GVTIDRFGHFLVLQLLSAGAEYQRAALISALQTCYPDCAIYDRSDVAVRKKEGMALTQGP
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCEEECCCCCEEECCC
VTGELPPALLPIEEHGMKLLVDIQGGHKTGYYLDQRDSRLATRRYVENQRVLNCFSYTGG
CCCCCCCCCCCHHHCCCEEEEEECCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCH
FAVSALMGGCRQVVSVDTSQDALDIARQNVELNQLDLSKAEFVRDDVFKLLRAYREHGEK
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCE
FDVIIMDPPKFVENKSQLMGACRGYKDINMLAIQLLNPGGILLTFSCSGLMTSDLFQKII
EEEEEECCCHHHCCHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHH
ADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFACRVM
HHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA