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The map label for this gene is cysL [H]

Identifier: 16764221

GI number: 16764221

Start: 932023

End: 932913

Strand: Reverse

Name: cysL [H]

Synonym: STM0859

Alternate gene names: 16764221

Gene position: 932913-932023 (Counterclockwise)

Preceding gene: 16764222

Following gene: 16764214

Centisome position: 19.21

GC content: 40.18

Gene sequence:

>891_bases
ATGCACTTTGATATAAAAGATTTAAAATTATTCCTTGCTATCAGTGAAGAAGGGAGCATAACACGTGGAGCGGAAAAAAC
TTACATGTCTGCCGCATCTGGTAGCGTGCGTATACGTAATCTTGAACAGAGTATCAATCTGAGATTGCTGTATCGTAGTA
GCAAGGGGATAACGTTATCCTCTGCTGGTGAAACATTTAAACACCACGCCAGCCTTATTGTGAATCAGATGGAAATGTTA
CGTAGCGATATGCAGGAGCATGTTAAAGGCATCAAAGGACATGTCAGAATATTTTCCAGTATAACTGCTATTGCAGAGTA
TCTCCCCCCATTGTTAAGCCATTTCCTTACACTATATAACGATGTGAATATTGATCTCAGGGAGTATCCAAGTCCGGAAA
TTGTACGTTCTGTTATTGAGGGGAAAACTGATCTCGGAATTGTTGCTGGTAATATTACAACAGATAATCTTGAAATACTG
CCGTTCCGTACTGATAGGCTAGTGCTTATCACCGCGACAGACCATCCCTTTGCTGACAGAAAAAGCGTGACCTTTCATGA
ATTAATTCAATATGAACAAATCAGCCTGCCTGAAGGGACAGGAATGTTTGAATTTATCCGAAAAAAGGCAGAACTAGTAC
CATCTAAACTGAAAATTAGAATACAGGTCGGCAACTTTGAAACATTTTGCCGTATGGTTGAAGCAAAAGTGGGCATTGGT
ATAATGCCTCACTCTGCTGCATCCCGTTTGCGTCAGTTGCATGAATTTTCGATTATTGATCTTGATGAATTTTGGTCATT
AAGAAAATTACAAATCTGCGCCAGAAGCTTTGACAAATTACCCGGCTTCACACAAAAACTGGTTACTATGCTCACAGCTC
AGACTGAGTGA

Upstream 100 bases:

>100_bases
TCTCGACGTGACAGGAATTCGGATAGGCCGAAATACTCAGTCTGACTGAGTCTATAAAAAAGCAGCACACTGTATTATAC
GTTAATTATGAGCCACAACG

Downstream 100 bases:

>100_bases
TAGCGTTACATCCCTGTATAATTTGGTCCGTTGCCACCTTCAGGCGTAGTCCAGGTTATATTTTGTTTAGGATCTTTAAT
GTCACAAACTTTACAGTGAA

Product: transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MHFDIKDLKLFLAISEEGSITRGAEKTYMSAASGSVRIRNLEQSINLRLLYRSSKGITLSSAGETFKHHASLIVNQMEML
RSDMQEHVKGIKGHVRIFSSITAIAEYLPPLLSHFLTLYNDVNIDLREYPSPEIVRSVIEGKTDLGIVAGNITTDNLEIL
PFRTDRLVLITATDHPFADRKSVTFHELIQYEQISLPEGTGMFEFIRKKAELVPSKLKIRIQVGNFETFCRMVEAKVGIG
IMPHSAASRLRQLHEFSIIDLDEFWSLRKLQICARSFDKLPGFTQKLVTMLTAQTE

Sequences:

>Translated_296_residues
MHFDIKDLKLFLAISEEGSITRGAEKTYMSAASGSVRIRNLEQSINLRLLYRSSKGITLSSAGETFKHHASLIVNQMEML
RSDMQEHVKGIKGHVRIFSSITAIAEYLPPLLSHFLTLYNDVNIDLREYPSPEIVRSVIEGKTDLGIVAGNITTDNLEIL
PFRTDRLVLITATDHPFADRKSVTFHELIQYEQISLPEGTGMFEFIRKKAELVPSKLKIRIQVGNFETFCRMVEAKVGIG
IMPHSAASRLRQLHEFSIIDLDEFWSLRKLQICARSFDKLPGFTQKLVTMLTAQTE
>Mature_296_residues
MHFDIKDLKLFLAISEEGSITRGAEKTYMSAASGSVRIRNLEQSINLRLLYRSSKGITLSSAGETFKHHASLIVNQMEML
RSDMQEHVKGIKGHVRIFSSITAIAEYLPPLLSHFLTLYNDVNIDLREYPSPEIVRSVIEGKTDLGIVAGNITTDNLEIL
PFRTDRLVLITATDHPFADRKSVTFHELIQYEQISLPEGTGMFEFIRKKAELVPSKLKIRIQVGNFETFCRMVEAKVGIG
IMPHSAASRLRQLHEFSIIDLDEFWSLRKLQICARSFDKLPGFTQKLVTMLTAQTE

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693105, Length=243, Percent_Identity=23.8683127572016, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787879, Length=269, Percent_Identity=27.8810408921933, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI157672245, Length=183, Percent_Identity=28.4153005464481, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790208, Length=246, Percent_Identity=25.609756097561, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788748, Length=245, Percent_Identity=23.6734693877551, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786713, Length=256, Percent_Identity=24.21875, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788887, Length=190, Percent_Identity=27.8947368421053, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787530, Length=230, Percent_Identity=25.2173913043478, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787601, Length=149, Percent_Identity=26.8456375838926, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788481, Length=242, Percent_Identity=22.3140495867769, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787806, Length=270, Percent_Identity=22.5925925925926, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1790399, Length=250, Percent_Identity=21.6, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790262, Length=244, Percent_Identity=23.3606557377049, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788297, Length=253, Percent_Identity=24.1106719367589, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI87081904, Length=229, Percent_Identity=23.5807860262009, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33554; Mature: 33554

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFDIKDLKLFLAISEEGSITRGAEKTYMSAASGSVRIRNLEQSINLRLLYRSSKGITLS
CCCCHHEEEEEEEECCCCCCCCCCHHHHHHHCCCCEEEEEHHCCCCEEEEEECCCCEEEE
SAGETFKHHASLIVNQMEMLRSDMQEHVKGIKGHVRIFSSITAIAEYLPPLLSHFLTLYN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVNIDLREYPSPEIVRSVIEGKTDLGIVAGNITTDNLEILPFRTDRLVLITATDHPFADR
CCCCCHHCCCCHHHHHHHHCCCCCCEEEECCEEECCEEEEEECCCCEEEEEECCCCCCCC
KSVTFHELIQYEQISLPEGTGMFEFIRKKAELVPSKLKIRIQVGNFETFCRMVEAKVGIG
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHCCC
IMPHSAASRLRQLHEFSIIDLDEFWSLRKLQICARSFDKLPGFTQKLVTMLTAQTE
CCCCHHHHHHHHHHHCCEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MHFDIKDLKLFLAISEEGSITRGAEKTYMSAASGSVRIRNLEQSINLRLLYRSSKGITLS
CCCCHHEEEEEEEECCCCCCCCCCHHHHHHHCCCCEEEEEHHCCCCEEEEEECCCCEEEE
SAGETFKHHASLIVNQMEMLRSDMQEHVKGIKGHVRIFSSITAIAEYLPPLLSHFLTLYN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVNIDLREYPSPEIVRSVIEGKTDLGIVAGNITTDNLEILPFRTDRLVLITATDHPFADR
CCCCCHHCCCCHHHHHHHHCCCCCCEEEECCEEECCEEEEEECCCCEEEEEECCCCCCCC
KSVTFHELIQYEQISLPEGTGMFEFIRKKAELVPSKLKIRIQVGNFETFCRMVEAKVGIG
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHCCC
IMPHSAASRLRQLHEFSIIDLDEFWSLRKLQICARSFDKLPGFTQKLVTMLTAQTE
CCCCHHHHHHHHHHHCCEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]