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The map label for this gene is pnuC

Identifier: 16764122

GI number: 16764122

Start: 821301

End: 822020

Strand: Direct

Name: pnuC

Synonym: STM0757

Alternate gene names: 16764122

Gene position: 821301-822020 (Clockwise)

Preceding gene: 16764121

Following gene: 16764125

Centisome position: 16.91

GC content: 50.69

Gene sequence:

>720_bases
ATGGATTTTTTTAGTACGCACAACATACTGATTCATATTCCGATTGGCGCTGGCGGGTACGATCTCTCGTGGATCGAAGC
GGTAGGAACCATCGCCGGCCTGCTCTGTATTTGGCTTGCCAGTCTGGAGAAGATCAGCAACTACTTTTTTGGACTGGTTA
ACGTTACCCTGTTTGCGATTATTTTCTTTCAGATCCAGCTTTATGCCAGCCTGTTGCTGCAACTCTTTTTCTTTGCCGCC
AATATTTATGGCTGGTATGCGTGGTCGCGGCAAACAAAGGATAATCAAGCCGAGCTTAAAATCCGCTGGCTGCCGTTGCC
AAAAGCAATGGCATGGCTGGCGATATGTGTGATAGCTATCGGTTTGATGACGCGATATATCGATCCCGTATTCGCCGTCC
TGACGCGCGTGGCCGTCGCCATTATGCAGATGCTGGGGTTACAGGTGACAATGCCCGTACTGCAACCGGACGCTTTCCCG
TTCTGGGACTCTTGCATGATGGTGCTGTCTATCGTGGCGATGATTCTGATGACACGCAAATATGTCGAAAACTGGCTCCT
GTGGGTGATAATCAACGTGATCAGTGTGGTGATTTTTGCTTTGCAGGGCGTCTATGCGATGTCGCTGGAATATCTGATCC
TGACATTTATCGCCGTGAACGGTAGCCGCCTGTGGATAAACAGCGCGCGGGAGCGAGGATCGCGCGCGCTTTCCCGTTAA

Upstream 100 bases:

>100_bases
TGATGCGGCGCTACGCGAGGGCGCATTACTGCCGCTCAACCGGATGCTGGATTTTGCGGCTACACTTCGGGCGTAAAGAC
GGAAATCTTTGGGGGAGAGA

Downstream 100 bases:

>100_bases
TGGTGATGAACATGCCCGGACGATGTCTGATTCAGATGACAGTCCGGGCCGTGACACACTTGATACTCCATCTGAATCGT
AGCGTGCGCAATATGGTATT

Product: nucleoside/purine/pyrimidine transporter

Products: nicotinamide nucleotide [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MDFFSTHNILIHIPIGAGGYDLSWIEAVGTIAGLLCIWLASLEKISNYFFGLVNVTLFAIIFFQIQLYASLLLQLFFFAA
NIYGWYAWSRQTKDNQAELKIRWLPLPKAMAWLAICVIAIGLMTRYIDPVFAVLTRVAVAIMQMLGLQVTMPVLQPDAFP
FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYLILTFIAVNGSRLWINSARERGSRALSR

Sequences:

>Translated_239_residues
MDFFSTHNILIHIPIGAGGYDLSWIEAVGTIAGLLCIWLASLEKISNYFFGLVNVTLFAIIFFQIQLYASLLLQLFFFAA
NIYGWYAWSRQTKDNQAELKIRWLPLPKAMAWLAICVIAIGLMTRYIDPVFAVLTRVAVAIMQMLGLQVTMPVLQPDAFP
FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYLILTFIAVNGSRLWINSARERGSRALSR
>Mature_239_residues
MDFFSTHNILIHIPIGAGGYDLSWIEAVGTIAGLLCIWLASLEKISNYFFGLVNVTLFAIIFFQIQLYASLLLQLFFFAA
NIYGWYAWSRQTKDNQAELKIRWLPLPKAMAWLAICVIAIGLMTRYIDPVFAVLTRVAVAIMQMLGLQVTMPVLQPDAFP
FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYLILTFIAVNGSRLWINSARERGSRALSR

Specific function: Required for nicotinamide riboside transport across the inner membrane

COG id: COG3201

COG function: function code H; Nicotinamide mononucleotide transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nicotinamide ribonucleoside (NR) uptake permease (TC 4.B.1) family

Homologues:

Organism=Escherichia coli, GI1786965, Length=239, Percent_Identity=88.7029288702929, Blast_Score=416, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PNUC_SALTY (P24520)

Other databases:

- EMBL:   M85180
- EMBL:   AE006468
- PIR:   D37753
- RefSeq:   NP_459737.1
- GeneID:   1252277
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM0757
- NMPDR:   fig|99287.1.peg.725
- HOGENOM:   HBG678458
- OMA:   GGGYAMS
- ProtClustDB:   PRK15397
- BioCyc:   STYP99287:STM0757-MONOMER
- GO:   GO:0006810
- InterPro:   IPR006419
- TIGRFAMs:   TIGR01528

Pfam domain/function: PF04973 NMN_transporter

EC number: NA

Molecular weight: Translated: 27191; Mature: 27191

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xd35bb18)-; HASH(0xcd5bee8)-; HASH(0xcf68440)-; HASH(0xcf95cc4)-; HASH(0xc5a31e8)-; HASH(0xd37e258)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFFSTHNILIHIPIGAGGYDLSWIEAVGTIAGLLCIWLASLEKISNYFFGLVNVTLFAI
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFFQIQLYASLLLQLFFFAANIYGWYAWSRQTKDNQAELKIRWLPLPKAMAWLAICVIAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHH
GLMTRYIDPVFAVLTRVAVAIMQMLGLQVTMPVLQPDAFPFWDSCMMVLSIVAMILMTRK
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
YVENWLLWVIINVISVVIFALQGVYAMSLEYLILTFIAVNGSRLWINSARERGSRALSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCH
>Mature Secondary Structure
MDFFSTHNILIHIPIGAGGYDLSWIEAVGTIAGLLCIWLASLEKISNYFFGLVNVTLFAI
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFFQIQLYASLLLQLFFFAANIYGWYAWSRQTKDNQAELKIRWLPLPKAMAWLAICVIAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHH
GLMTRYIDPVFAVLTRVAVAIMQMLGLQVTMPVLQPDAFPFWDSCMMVLSIVAMILMTRK
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
YVENWLLWVIINVISVVIFALQGVYAMSLEYLILTFIAVNGSRLWINSARERGSRALSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nicotinamide nucleotide [Periplasm] [C]

Specific reaction: nicotinamide nucleotide [Periplasm] = nicotinamide nucleotide [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2198247; 11677609