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The map label for this gene is tolA [H]

Identifier: 16764117

GI number: 16764117

Start: 814617

End: 815840

Strand: Direct

Name: tolA [H]

Synonym: STM0747

Alternate gene names: 16764117

Gene position: 814617-815840 (Clockwise)

Preceding gene: 16764116

Following gene: 16764118

Centisome position: 16.77

GC content: 58.82

Gene sequence:

>1224_bases
GTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAACGGGCGATAATTATTTCAGCCGTGCTGCATATCATCTTATTTGC
AGTGCTGATCTGGAGTTCGTTTGATGAGCATATAGAGGCTTCTGCCGGCGGCGGCGGTGGTTCCGCTATCGACGCGGTGA
TGGTCGATCCTGGCGCCGTTGTGCAGCAGTACAACCGTCAGCAGGATCAACAGGCCAGCGCCAGACGCGCGGAAGAAGAG
CGTAAAAAGCTGCAACAGCAGCAAGCAGAGGAGCTGCAGCAGAAGCAGGCTGCTGAACAGGAGCGGTTGAAACAACTTGA
GAAAGAACGTTTAGCGGCTCAGGAGCAGCAAAAGCAGGCTGAAGAAGCGGCAAAACTGGCGCAACAGCAGCAGCAACAGG
CCGAAGAAGCGGCGAAAGCGGCGGCGGACGCGAAGAAAAAAGCGGAGGCCGAGGCGGCGAAAGCGGCGGCGGACGCGAAG
AAGAAAGCGGAAGCCGAAGCGGTAAAAGCGGCAGCGGACGCGAAGAAGAAAGCGGAAGCCGAAGCGGCGAAAGCGGCGGC
GGACGCGAAGAAGAAAGCAGAGGCCGAAGCGGCGAAAGCGGCGGCGGAGGCGAAGAAGAAAGCGGAAGCCGAAGCGGCGA
AAGCGGCGGCGGAGGCGAAGAAGAAAGCGGATGCCGAGGCGGCGAAAGCGGCGGCGGAGGCGAAGAAGAAAGCGGATGCC
GCGGCGGCGAAAGCGGCGGCGGAGGCGAAGAAGAAAGCGGATGCCGCGGCGGCGAAAGCAGCGGCGGACGCTAAGAAGAA
AGCGGCTGCCGAAAAAGCGGCCGCCGCAGAAGGCGTCGACGATCTGCTTGGCGATCTCAGCTCGGGTAAGAATGCGCCGA
AAACCGGCGGCGGCGCGAAAGGGAATGGTCAGCCATCGAAAGATAGCGGTACATCGGGCGCTAACGGTGGGGCGACAGGC
GCTGATATCAGCGCCTACGCGAAACAGATTCAGGTCGCCATTCAGAGCCGTCTGTACGATGCGAGCCTGTATCAGGGCAA
ACAATGTGTCTTGCATATTAGCCTGGCGCCGGATGGCTCATTAAAAAGCATTACGTCTGAGGGCGGCGATCCGGCGCTTT
GTCAGGCGGCGTTAATGGCGGCAAAAACCGCGAAAATTCCTAAACCGCCAAGCCAGGCTGTTTATGAGAAAATAAAGGAT
GCCAAACTAGACTTTAAACTGTAG

Upstream 100 bases:

>100_bases
GTAAAATCGGTTGGCTTAATGACGCAGCCAATCTGAAGTCTGCGTCCCGTTGGCTTGAAAGACGGCGAGTAACAGGCGAA
CAGTTTTTTGGGAACCGAGA

Downstream 100 bases:

>100_bases
ATACTTTTCTTTATGGAAGTTAGAGCAGTTGAGTTGTTACTGGTCCTGGTGGTTTTGTGTATTTGAGTTTGTTAACATTC
TGCTAAATTATCGTGGGTCG

Product: cell envelope integrity inner membrane protein TolA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MSKATEQNDKLKRAIIISAVLHIILFAVLIWSSFDEHIEASAGGGGGSAIDAVMVDPGAVVQQYNRQQDQQASARRAEEE
RKKLQQQQAEELQQKQAAEQERLKQLEKERLAAQEQQKQAEEAAKLAQQQQQQAEEAAKAAADAKKKAEAEAAKAAADAK
KKAEAEAVKAAADAKKKAEAEAAKAAADAKKKAEAEAAKAAAEAKKKAEAEAAKAAAEAKKKADAEAAKAAAEAKKKADA
AAAKAAAEAKKKADAAAAKAAADAKKKAAAEKAAAAEGVDDLLGDLSSGKNAPKTGGGAKGNGQPSKDSGTSGANGGATG
ADISAYAKQIQVAIQSRLYDASLYQGKQCVLHISLAPDGSLKSITSEGGDPALCQAALMAAKTAKIPKPPSQAVYEKIKD
AKLDFKL

Sequences:

>Translated_407_residues
MSKATEQNDKLKRAIIISAVLHIILFAVLIWSSFDEHIEASAGGGGGSAIDAVMVDPGAVVQQYNRQQDQQASARRAEEE
RKKLQQQQAEELQQKQAAEQERLKQLEKERLAAQEQQKQAEEAAKLAQQQQQQAEEAAKAAADAKKKAEAEAAKAAADAK
KKAEAEAVKAAADAKKKAEAEAAKAAADAKKKAEAEAAKAAAEAKKKAEAEAAKAAAEAKKKADAEAAKAAAEAKKKADA
AAAKAAAEAKKKADAAAAKAAADAKKKAAAEKAAAAEGVDDLLGDLSSGKNAPKTGGGAKGNGQPSKDSGTSGANGGATG
ADISAYAKQIQVAIQSRLYDASLYQGKQCVLHISLAPDGSLKSITSEGGDPALCQAALMAAKTAKIPKPPSQAVYEKIKD
AKLDFKL
>Mature_406_residues
SKATEQNDKLKRAIIISAVLHIILFAVLIWSSFDEHIEASAGGGGGSAIDAVMVDPGAVVQQYNRQQDQQASARRAEEER
KKLQQQQAEELQQKQAAEQERLKQLEKERLAAQEQQKQAEEAAKLAQQQQQQAEEAAKAAADAKKKAEAEAAKAAADAKK
KAEAEAVKAAADAKKKAEAEAAKAAADAKKKAEAEAAKAAAEAKKKAEAEAAKAAAEAKKKADAEAAKAAAEAKKKADAA
AAKAAAEAKKKADAAAAKAAADAKKKAAAEKAAAAEGVDDLLGDLSSGKNAPKTGGGAKGNGQPSKDSGTSGANGGATGA
DISAYAKQIQVAIQSRLYDASLYQGKQCVLHISLAPDGSLKSITSEGGDPALCQAALMAAKTAKIPKPPSQAVYEKIKDA
KLDFKL

Specific function: Involved in the tonB-independent uptake of group A colicins (colicins A, E1, E2, E3, and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria. Also involved in the translocation of bacteriophage DNA [H]

COG id: COG3064

COG function: function code M; Membrane protein involved in colicin uptake

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786960, Length=128, Percent_Identity=81.25, Blast_Score=186, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014161 [H]

Pfam domain/function: PF06519 TolA [H]

EC number: NA

Molecular weight: Translated: 41865; Mature: 41734

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SKATEQNDKLKRAIIISAVLHIILFAVLIWSSFDEHIEASAGGGGGSAIDAVMVDPGAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHEECCHHH
VQQYNRQQDQQASARRAEEERKKLQQQQAEELQQKQAAEQERLKQLEKERLAAQEQQKQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEAAKLAQQQQQQAEEAAKAAADAKKKAEAEAAKAAADAKKKAEAEAVKAAADAKKKAEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAAKAAADAKKKAEAEAAKAAAEAKKKAEAEAAKAAAEAKKKADAEAAKAAAEAKKKADA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAKAAAEAKKKADAAAAKAAADAKKKAAAEKAAAAEGVDDLLGDLSSGKNAPKTGGGAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCC
GNGQPSKDSGTSGANGGATGADISAYAKQIQVAIQSRLYDASLYQGKQCVLHISLAPDGS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
LKSITSEGGDPALCQAALMAAKTAKIPKPPSQAVYEKIKDAKLDFKL
HHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2687247; 8905232; 9278503; 2068069; 8978668; 10404600 [H]