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The map label for this gene is shiA [H]

Identifier: 16763602

GI number: 16763602

Start: 247951

End: 249252

Strand: Direct

Name: shiA [H]

Synonym: STM0212

Alternate gene names: 16763602

Gene position: 247951-249252 (Clockwise)

Preceding gene: 16763600

Following gene: 16763606

Centisome position: 5.1

GC content: 51.23

Gene sequence:

>1302_bases
ATGTCCGGAACGTATAATGCTACTATTCGCCGGGTGGTGGTTTCCGCCTGGATAGGAAACTCCATTGAATATTATGATTT
TCTATTATACGGCCTGGCAAGCGCGCTGGTATTTGGCCCGCTTTTTTTTCCTGGCGCCAGCCCATTGACTGCAACGTTAT
CTTCTTTTGCCAGTTTCGGCGTGGGATTTATCTCCCGACCGCTGGGCGCGCTATTTTTTGGCAACAGGGGAGATACGCTT
GGGCGTAAAAACACGTTATTAATTACGTTAGGGGGGATGGGCGCGGTCACGTTTCTCATCGGTTGCCTGCCTTCTTACGC
CAGTATTGGCGCGCTGGCGCCTGCTTTACTGGTTATTCTGCGTTTTCTACAGGGATTTATGGTTGGAGGCGAATGGGGAG
GCGCCATGTTGATGGTCGTGGAATATGCTGCTGGAAAGCACCGGGGGCGGTTAAGCGCGCTATCGCAAACAGGAGGACTC
ACGGGACAATTATTGGCGACAGGCGTATTTATAGTCGTGACCCAATTACCGCAGGAGGCGTTGTTATCGTGGGGATGGCG
TATTCCTTTTCTGCTCAGTGCGCTTTTGGTGTTGCCTGGATTATATATGCGTCATCGTCTGGATGAGACGCCTGTCTTCC
GTGCGTTTAAAAAGCAGCAGGCCATTAATCATAGGCAGCAAAGAGAAGAAAGGCCGGTGGTCAAAGTAGTACGAGAACAA
TGGCGCAGCATTTTGCTGATTATAATTCTGCGTTTTGCGGAAAGCGTTCCCTTTTTTCTTGCTACCGTCTTCGCGGTTTC
CTGGGCGACGACGCAGCTTGGGATTGCCAGCCTGACTATTCTGTACATTGTAATGTTCACCTGTCTGCTGGCGTATCCCA
TGCACGTGCTATTTGGCATCATGTCGGATCGGCGTGGCTGCCGTCAGGTGTATATTTTCGGCGCGCTTTTTGTCGCGGCA
ATGGCTTTTCCCTTTTTCTGGCTGCTGGAAAGCCGTTCGCTCATACTGATGACAATGGGCTACGTTCTGCTTATTAACAT
TGGTCATAATTCACTGAATGCCGTGCAACCTTCTTTTTTCGCCGGACTGTTTCATCCGCCTGTACGCTACAGCGGTTCAT
CAATTGGCGCTCAGTTGGGGGCGGTTGTGGCAGGGGGATTCACGCCGTTTATCGCTAAAGCGTTAAGCGCAGTCTATGAT
AACAGTTGGGCGTTGGTTGCCGGTTATGTCGTTTTGACTGCGTTGGCTTCGGCGTTTGCGGCAAAGATTGCGCCGGAAAC
CGTGTTACCGCATTCGCCGTAA

Upstream 100 bases:

>100_bases
AAGTAATTCAACGCGATATTCCGTTTATAGAGAAAGCGTTTGAAGTCAGTGTGGGAAATTGACCGGAGGCAGTGGTCGAT
AACCTTGAGAGGCGGAGTCT

Downstream 100 bases:

>100_bases
TGCAAAGGGGGGCTGTCGCCCCCGCTTTTCACTCGCTTAATCAATAGTGCGCAATAATTCGTTAATGCCCACTTTACCAC
GCGTTTTGGCGTCGACTTTT

Product: inner membrane protein

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSGTYNATIRRVVVSAWIGNSIEYYDFLLYGLASALVFGPLFFPGASPLTATLSSFASFGVGFISRPLGALFFGNRGDTL
GRKNTLLITLGGMGAVTFLIGCLPSYASIGALAPALLVILRFLQGFMVGGEWGGAMLMVVEYAAGKHRGRLSALSQTGGL
TGQLLATGVFIVVTQLPQEALLSWGWRIPFLLSALLVLPGLYMRHRLDETPVFRAFKKQQAINHRQQREERPVVKVVREQ
WRSILLIIILRFAESVPFFLATVFAVSWATTQLGIASLTILYIVMFTCLLAYPMHVLFGIMSDRRGCRQVYIFGALFVAA
MAFPFFWLLESRSLILMTMGYVLLINIGHNSLNAVQPSFFAGLFHPPVRYSGSSIGAQLGAVVAGGFTPFIAKALSAVYD
NSWALVAGYVVLTALASAFAAKIAPETVLPHSP

Sequences:

>Translated_433_residues
MSGTYNATIRRVVVSAWIGNSIEYYDFLLYGLASALVFGPLFFPGASPLTATLSSFASFGVGFISRPLGALFFGNRGDTL
GRKNTLLITLGGMGAVTFLIGCLPSYASIGALAPALLVILRFLQGFMVGGEWGGAMLMVVEYAAGKHRGRLSALSQTGGL
TGQLLATGVFIVVTQLPQEALLSWGWRIPFLLSALLVLPGLYMRHRLDETPVFRAFKKQQAINHRQQREERPVVKVVREQ
WRSILLIIILRFAESVPFFLATVFAVSWATTQLGIASLTILYIVMFTCLLAYPMHVLFGIMSDRRGCRQVYIFGALFVAA
MAFPFFWLLESRSLILMTMGYVLLINIGHNSLNAVQPSFFAGLFHPPVRYSGSSIGAQLGAVVAGGFTPFIAKALSAVYD
NSWALVAGYVVLTALASAFAAKIAPETVLPHSP
>Mature_432_residues
SGTYNATIRRVVVSAWIGNSIEYYDFLLYGLASALVFGPLFFPGASPLTATLSSFASFGVGFISRPLGALFFGNRGDTLG
RKNTLLITLGGMGAVTFLIGCLPSYASIGALAPALLVILRFLQGFMVGGEWGGAMLMVVEYAAGKHRGRLSALSQTGGLT
GQLLATGVFIVVTQLPQEALLSWGWRIPFLLSALLVLPGLYMRHRLDETPVFRAFKKQQAINHRQQREERPVVKVVREQW
RSILLIIILRFAESVPFFLATVFAVSWATTQLGIASLTILYIVMFTCLLAYPMHVLFGIMSDRRGCRQVYIFGALFVAAM
AFPFFWLLESRSLILMTMGYVLLINIGHNSLNAVQPSFFAGLFHPPVRYSGSSIGAQLGAVVAGGFTPFIAKALSAVYDN
SWALVAGYVVLTALASAFAAKIAPETVLPHSP

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=419, Percent_Identity=41.0501193317422, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI1790550, Length=403, Percent_Identity=34.2431761786601, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1789941, Length=399, Percent_Identity=33.8345864661654, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1788942, Length=405, Percent_Identity=30.8641975308642, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI87082404, Length=403, Percent_Identity=24.5657568238213, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46984; Mature: 46853

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTYNATIRRVVVSAWIGNSIEYYDFLLYGLASALVFGPLFFPGASPLTATLSSFASFG
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VGFISRPLGALFFGNRGDTLGRKNTLLITLGGMGAVTFLIGCLPSYASIGALAPALLVIL
HHHHHCCHHHHEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFLQGFMVGGEWGGAMLMVVEYAAGKHRGRLSALSQTGGLTGQLLATGVFIVVTQLPQEA
HHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHH
LLSWGWRIPFLLSALLVLPGLYMRHRLDETPVFRAFKKQQAINHRQQREERPVVKVVREQ
HHHCCCCHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
WRSILLIIILRFAESVPFFLATVFAVSWATTQLGIASLTILYIVMFTCLLAYPMHVLFGI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSDRRGCRQVYIFGALFVAAMAFPFFWLLESRSLILMTMGYVLLINIGHNSLNAVQPSFF
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCCCCCCHHHH
AGLFHPPVRYSGSSIGAQLGAVVAGGFTPFIAKALSAVYDNSWALVAGYVVLTALASAFA
HHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AKIAPETVLPHSP
HHCCCCCCCCCCC
>Mature Secondary Structure 
SGTYNATIRRVVVSAWIGNSIEYYDFLLYGLASALVFGPLFFPGASPLTATLSSFASFG
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VGFISRPLGALFFGNRGDTLGRKNTLLITLGGMGAVTFLIGCLPSYASIGALAPALLVIL
HHHHHCCHHHHEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFLQGFMVGGEWGGAMLMVVEYAAGKHRGRLSALSQTGGLTGQLLATGVFIVVTQLPQEA
HHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHH
LLSWGWRIPFLLSALLVLPGLYMRHRLDETPVFRAFKKQQAINHRQQREERPVVKVVREQ
HHHCCCCHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
WRSILLIIILRFAESVPFFLATVFAVSWATTQLGIASLTILYIVMFTCLLAYPMHVLFGI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSDRRGCRQVYIFGALFVAAMAFPFFWLLESRSLILMTMGYVLLINIGHNSLNAVQPSFF
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCCCCCCHHHH
AGLFHPPVRYSGSSIGAQLGAVVAGGFTPFIAKALSAVYDNSWALVAGYVVLTALASAFA
HHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AKIAPETVLPHSP
HHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]