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The map label for this gene is htrA

Identifier: 16763599

GI number: 16763599

Start: 244492

End: 245919

Strand: Direct

Name: htrA

Synonym: STM0209

Alternate gene names: 16763599

Gene position: 244492-245919 (Clockwise)

Preceding gene: 16763598

Following gene: 16763600

Centisome position: 5.03

GC content: 54.9

Gene sequence:

>1428_bases
ATGAAAAAAACCACATTAGCAATGAGTGCACTGGCTCTGAGTTTAGGTTTGGCATTGTCGCCTCTGTCTGCCACGGCGGC
TGAAACGTCCTCTTCAGCAATGACTGCCCAGCAGATGCCAAGCCTGGCACCGATGCTCGAAAAAGTGATGCCATCGGTGG
TCAGTATTAATGTTGAAGGTAGCACCACGGTGAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTTGGCGATGAC
TCCCCGTTCTGCCAGGACGGTTCTCCGTTCCAGAATTCTCCGTTCTGCCAGGGCGGCGGTAACGGCGGCAACGGCGGTCA
ACAACAGAAATTCATGGCGCTGGGCTCCGGCGTAATTATTGACGCCGCGAAGGGCTACGTCGTCACCAACAACCACGTGG
TTGATAACGCCAGCGTGATTAAAGTACAGCTTAGCGATGGGCGTAAATTCGATGCTAAAGTGGTGGGCAAAGATCCGCGT
TCTGATATCGCGCTGATTCAAATTCAGAATCCGAAGAACCTGACGGCGATTAAGCTGGCGGACTCCGACGCGCTGCGCGT
GGGGGATTATACCGTCGCTATTGGTAACCCGTTTGGTCTGGGCGAAACGGTGACGTCAGGTATCGTTTCGGCGCTGGGGC
GTAGCGGCCTGAACGTAGAAAATTACGAGAACTTTATTCAGACCGACGCCGCGATTAACCGTGGTAACTCCGGCGGCGCG
CTGGTGAACCTGAACGGTGAGCTGATCGGTATTAACACCGCGATTCTGGCGCCGGACGGCGGCAACATCGGTATCGGCTT
CGCTATCCCCAGTAACATGGTGAAAAACCTGACGTCGCAGATGGTGGAATACGGCCAGGTGAAACGCGGCGAACTGGGGA
TCATGGGGACTGAGCTGAATTCCGAATTGGCGAAAGCGATGAAAGTCGACGCCCAGCGAGGCGCGTTCGTCAGCCAGGTG
ATGCCGAATTCGTCCGCGGCGAAAGCGGGTATCAAAGCCGGGGATGTCATTACCTCGCTGAACGGTAAACCGATCAGCAG
CTTTGCGGCGCTGCGCGCTCAGGTCGGCACTATGCCGGTCGGCAGCAAAATCAGCCTCGGTCTGCTGCGTGAAGGTAAAG
CGATTACGGTGAATCTGGAACTGCAGCAGAGCAGCCAGAGTCAGGTTGATTCCAGCACCATCTTCAGCGGGATTGAAGGC
GCTGAAATGAGCAATAAAGGCCAGGATAAAGGCGTTGTGGTGAGCAGCGTGAAAGCGAACTCACCCGCCGCGCAAATTGG
CCTCAAAAAAGGCGATGTGATTATCGGCGCTAACCAGCAGCCGGTGAAAAATATCGCCGAGCTGCGTAAGATTCTCGACA
GCAAGCCGTCGGTTCTGGCGCTGAATATTCAGCGTGGTGATAGTTCTATTTATTTGCTGATGCAGTAA

Upstream 100 bases:

>100_bases
TTTTACTTTTTCCAGAAACTTTATTCCGGAACTTCGCGTTATAAAATGAATCTGACGTACACAGCAATTTTGCGTTACCT
GTTAATCGAGATTGAAACAC

Downstream 100 bases:

>100_bases
TCACCTTTGTCCCCCTTCCGCCATGGAAGGGGGCAACACTTTTCTGTGAAACCCCCCACAACTCCATACTTATTTGCACC
GTTTTGTGCATTTGCACAAT

Product: serine endoprotease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKKTTLAMSALALSLGLALSPLSATAAETSSSAMTAQQMPSLAPMLEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDD
SPFCQDGSPFQNSPFCQGGGNGGNGGQQQKFMALGSGVIIDAAKGYVVTNNHVVDNASVIKVQLSDGRKFDAKVVGKDPR
SDIALIQIQNPKNLTAIKLADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNVENYENFIQTDAAINRGNSGGA
LVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNLTSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQV
MPNSSAAKAGIKAGDVITSLNGKPISSFAALRAQVGTMPVGSKISLGLLREGKAITVNLELQQSSQSQVDSSTIFSGIEG
AEMSNKGQDKGVVVSSVKANSPAAQIGLKKGDVIIGANQQPVKNIAELRKILDSKPSVLALNIQRGDSSIYLLMQ

Sequences:

>Translated_475_residues
MKKTTLAMSALALSLGLALSPLSATAAETSSSAMTAQQMPSLAPMLEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDD
SPFCQDGSPFQNSPFCQGGGNGGNGGQQQKFMALGSGVIIDAAKGYVVTNNHVVDNASVIKVQLSDGRKFDAKVVGKDPR
SDIALIQIQNPKNLTAIKLADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNVENYENFIQTDAAINRGNSGGA
LVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNLTSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQV
MPNSSAAKAGIKAGDVITSLNGKPISSFAALRAQVGTMPVGSKISLGLLREGKAITVNLELQQSSQSQVDSSTIFSGIEG
AEMSNKGQDKGVVVSSVKANSPAAQIGLKKGDVIIGANQQPVKNIAELRKILDSKPSVLALNIQRGDSSIYLLMQ
>Mature_475_residues
MKKTTLAMSALALSLGLALSPLSATAAETSSSAMTAQQMPSLAPMLEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDD
SPFCQDGSPFQNSPFCQGGGNGGNGGQQQKFMALGSGVIIDAAKGYVVTNNHVVDNASVIKVQLSDGRKFDAKVVGKDPR
SDIALIQIQNPKNLTAIKLADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNVENYENFIQTDAAINRGNSGGA
LVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNLTSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQV
MPNSSAAKAGIKAGDVITSLNGKPISSFAALRAQVGTMPVGSKISLGLLREGKAITVNLELQQSSQSQVDSSTIFSGIEG
AEMSNKGQDKGVVVSSVKANSPAAQIGLKKGDVIIGANQQPVKNIAELRKILDSKPSVLALNIQRGDSSIYLLMQ

Specific function: Serine protease that is required at high temperature. Involved in the degradation of damaged proteins. Shared specificity with hhoA/degQ

COG id: COG0265

COG function: function code O; Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PDZ (DHR) domains

Homologues:

Organism=Homo sapiens, GI4506141, Length=251, Percent_Identity=41.8326693227092, Blast_Score=165, Evalue=9e-41,
Organism=Homo sapiens, GI22129776, Length=263, Percent_Identity=38.0228136882129, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI24308541, Length=248, Percent_Identity=36.6935483870968, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI7019477, Length=264, Percent_Identity=36.7424242424242, Blast_Score=138, Evalue=1e-32,
Organism=Escherichia coli, GI1786356, Length=475, Percent_Identity=92.8421052631579, Blast_Score=832, Evalue=0.0,
Organism=Escherichia coli, GI1789629, Length=478, Percent_Identity=56.6945606694561, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI1789630, Length=347, Percent_Identity=38.328530259366, Blast_Score=205, Evalue=4e-54,
Organism=Drosophila melanogaster, GI24646839, Length=253, Percent_Identity=37.9446640316205, Blast_Score=139, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEGP_SALTY (P26982)

Other databases:

- EMBL:   X54548
- EMBL:   AE006468
- PIR:   S15337
- RefSeq:   NP_459214.1
- ProteinModelPortal:   P26982
- SMR:   P26982
- MEROPS:   S01.273
- PRIDE:   P26982
- GeneID:   1251727
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM0209
- HOGENOM:   HBG585708
- OMA:   TGINAWL
- ProtClustDB:   PRK10942
- BioCyc:   STYP99287:STM0209-MONOMER
- GO:   GO:0006508
- InterPro:   IPR001478
- InterPro:   IPR009003
- InterPro:   IPR011782
- InterPro:   IPR001254
- InterPro:   IPR001940
- PRINTS:   PR00834
- SMART:   SM00228
- TIGRFAMs:   TIGR02037

Pfam domain/function: PF00595 PDZ; PF00089 Trypsin; SSF50156 PDZ; SSF50494 Pept_Ser_Cys

EC number: 3.4.21.-

Molecular weight: Translated: 49316; Mature: 49316

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS50106 PDZ

Important sites: ACT_SITE 132-132 ACT_SITE 162-162 ACT_SITE 237-237

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTTLAMSALALSLGLALSPLSATAAETSSSAMTAQQMPSLAPMLEKVMPSVVSINVEG
CCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHCCHHHHHHHHHCCCEEEEEECC
STTVNTPRMPRNFQQFFGDDSPFCQDGSPFQNSPFCQGGGNGGNGGQQQKFMALGSGVII
CCEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEECCCEEE
DAAKGYVVTNNHVVDNASVIKVQLSDGRKFDAKVVGKDPRSDIALIQIQNPKNLTAIKLA
ECCCCEEEECCEEECCCEEEEEEECCCCEEEEEEECCCCCCCEEEEEECCCCCEEEEEEE
DSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNVENYENFIQTDAAINRGNSGGA
CCCCEEECCEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCE
LVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNLTSQMVEYGQVKRGELGIMGTELN
EEEECCEEEEEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEECCHH
SELAKAMKVDAQRGAFVSQVMPNSSAAKAGIKAGDVITSLNGKPISSFAALRAQVGTMPV
HHHHHHHHHHHHCCCHHHHHCCCCCHHHCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCC
GSKISLGLLREGKAITVNLELQQSSQSQVDSSTIFSGIEGAEMSNKGQDKGVVVSSVKAN
CCCEEEEEEECCCEEEEEEEECCCCCHHCCHHHHHCCCCCCCCCCCCCCCCEEEEEECCC
SPAAQIGLKKGDVIIGANQQPVKNIAELRKILDSKPSVLALNIQRGDSSIYLLMQ
CCHHHCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEEEC
>Mature Secondary Structure
MKKTTLAMSALALSLGLALSPLSATAAETSSSAMTAQQMPSLAPMLEKVMPSVVSINVEG
CCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHCCHHHHHHHHHCCCEEEEEECC
STTVNTPRMPRNFQQFFGDDSPFCQDGSPFQNSPFCQGGGNGGNGGQQQKFMALGSGVII
CCEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEECCCEEE
DAAKGYVVTNNHVVDNASVIKVQLSDGRKFDAKVVGKDPRSDIALIQIQNPKNLTAIKLA
ECCCCEEEECCEEECCCEEEEEEECCCCEEEEEEECCCCCCCEEEEEECCCCCEEEEEEE
DSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNVENYENFIQTDAAINRGNSGGA
CCCCEEECCEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCE
LVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNLTSQMVEYGQVKRGELGIMGTELN
EEEECCEEEEEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEECCHH
SELAKAMKVDAQRGAFVSQVMPNSSAAKAGIKAGDVITSLNGKPISSFAALRAQVGTMPV
HHHHHHHHHHHHCCCHHHHHCCCCCHHHCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCC
GSKISLGLLREGKAITVNLELQQSSQSQVDSSTIFSGIEGAEMSNKGQDKGVVVSSVKAN
CCCEEEEEEECCCEEEEEEEECCCCCHHCCHHHHHCCCCCCCCCCCCCCCCEEEEEECCC
SPAAQIGLKKGDVIIGANQQPVKNIAELRKILDSKPSVLALNIQRGDSSIYLLMQ
CCHHHCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1645840; 11677609