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The map label for this gene is btuF [H]

Identifier: 16763596

GI number: 16763596

Start: 241269

End: 242069

Strand: Reverse

Name: btuF [H]

Synonym: STM0206

Alternate gene names: 16763596

Gene position: 242069-241269 (Counterclockwise)

Preceding gene: 16763597

Following gene: 16763595

Centisome position: 4.98

GC content: 55.68

Gene sequence:

>801_bases
ATGGCTAAGCAAATGTTCAGGGCGCTGGTCGCCCTGCTGCTTACGCTCCCGGTGTGGCTTTACGCTGCGCCGCGCGTCAT
TACCCTCTCTCCCGCCAATACGGAGCTCGCTTTTGCCGCCGGGATTACGCCCGTTGGCGTCAGCAGCTATTCCGACTACC
CGCCCGAAGCGCAAAAAATAGAACAAGTCTCTACCTGGCAGGGAATGAATCTGGAACGCATTGTGGCGCTGAAGCCGGAT
CTGGTCGTCGCCTGGCGCGGCGGTAATGCGGAACGCCAGGTAAACCAACTGACGTCATTAGGTATTAAGGTCATGTGGGT
GGACGCGGTAACCATCGAACAGATCGCCGACACGCTCCGTCAACTGGCCGCCTGGAGCCCGCAGCCGGAAAAGGCTCAGC
AGGCAGCGCAGACATTGCTAAACGAGTACGCCGCGCTCAACGCCGAGTATGCCGGTAAAGCTAAAAAGCGCGTCTTTCTT
CAGTTTGGCATGAATCCTCTGTTTACCAGTGGTAAAGGGTCTATTCAACATCAGGTTTTGACGACCTGCGGCGGAGAAAA
CGTCTTTGCTGACAGCCGCGTGCCGTGGCCGCAAGTCAGCCGTGAGCAGGTGCTGGCAAGGCATCCCCAGGCCATTATCG
TGGCCGGAAAAGCGGGCGAAATTCTCAAAATTGAACAATACTGGGGAAACCTGTTAAAAATTCCGGTTATTCCGCTTAAC
AGCGACTGGTTTGAACGCGCAAGCCCGCGTATTATCCTCGCCGCAAAACAACTCTGTAATGCGCTTTCACAGGTGAATTA
G

Upstream 100 bases:

>100_bases
GTGGCCGATCAGCAATCGCACCTCAGCTTTGACGAATTCCTCGCGGTGGCGGCGAAACAGTCGACTCTGATGGTCGAAAC
GCTGGTACAGAAACTGGCGC

Downstream 100 bases:

>100_bases
AGCCTGTCTTACCAGGCTCCCAGTGGGGATTCGACAATGCTCGTCTATTGGCTGGATATTGTAGGCACGGCTGTATTTGC
TATCTCTGGCGTGTTGCTGG

Product: vitamin B12-transporter protein BtuF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MAKQMFRALVALLLTLPVWLYAAPRVITLSPANTELAFAAGITPVGVSSYSDYPPEAQKIEQVSTWQGMNLERIVALKPD
LVVAWRGGNAERQVNQLTSLGIKVMWVDAVTIEQIADTLRQLAAWSPQPEKAQQAAQTLLNEYAALNAEYAGKAKKRVFL
QFGMNPLFTSGKGSIQHQVLTTCGGENVFADSRVPWPQVSREQVLARHPQAIIVAGKAGEILKIEQYWGNLLKIPVIPLN
SDWFERASPRIILAAKQLCNALSQVN

Sequences:

>Translated_266_residues
MAKQMFRALVALLLTLPVWLYAAPRVITLSPANTELAFAAGITPVGVSSYSDYPPEAQKIEQVSTWQGMNLERIVALKPD
LVVAWRGGNAERQVNQLTSLGIKVMWVDAVTIEQIADTLRQLAAWSPQPEKAQQAAQTLLNEYAALNAEYAGKAKKRVFL
QFGMNPLFTSGKGSIQHQVLTTCGGENVFADSRVPWPQVSREQVLARHPQAIIVAGKAGEILKIEQYWGNLLKIPVIPLN
SDWFERASPRIILAAKQLCNALSQVN
>Mature_265_residues
AKQMFRALVALLLTLPVWLYAAPRVITLSPANTELAFAAGITPVGVSSYSDYPPEAQKIEQVSTWQGMNLERIVALKPDL
VVAWRGGNAERQVNQLTSLGIKVMWVDAVTIEQIADTLRQLAAWSPQPEKAQQAAQTLLNEYAALNAEYAGKAKKRVFLQ
FGMNPLFTSGKGSIQHQVLTTCGGENVFADSRVPWPQVSREQVLARHPQAIIVAGKAGEILKIEQYWGNLLKIPVIPLNS
DWFERASPRIILAAKQLCNALSQVN

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=266, Percent_Identity=81.203007518797, Blast_Score=445, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 29284; Mature: 29153

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQMFRALVALLLTLPVWLYAAPRVITLSPANTELAFAAGITPVGVSSYSDYPPEAQKI
CHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCHHHHH
EQVSTWQGMNLERIVALKPDLVVAWRGGNAERQVNQLTSLGIKVMWVDAVTIEQIADTLR
HHHHHCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHCCEEEEEECHHHHHHHHHHHH
QLAAWSPQPEKAQQAAQTLLNEYAALNAEYAGKAKKRVFLQFGMNPLFTSGKGSIQHQVL
HHHCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCHHHEEEEEECCCEEEECCCCCHHHHHH
TTCGGENVFADSRVPWPQVSREQVLARHPQAIIVAGKAGEILKIEQYWGNLLKIPVIPLN
HHCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCEEEEHHHHCCEEEEEEEECC
SDWFERASPRIILAAKQLCNALSQVN
CHHHHHCCCEEEEHHHHHHHHHHHCC
>Mature Secondary Structure 
AKQMFRALVALLLTLPVWLYAAPRVITLSPANTELAFAAGITPVGVSSYSDYPPEAQKI
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCHHHHH
EQVSTWQGMNLERIVALKPDLVVAWRGGNAERQVNQLTSLGIKVMWVDAVTIEQIADTLR
HHHHHCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHCCEEEEEECHHHHHHHHHHHH
QLAAWSPQPEKAQQAAQTLLNEYAALNAEYAGKAKKRVFLQFGMNPLFTSGKGSIQHQVL
HHHCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCHHHEEEEEECCCEEEECCCCCHHHHHH
TTCGGENVFADSRVPWPQVSREQVLARHPQAIIVAGKAGEILKIEQYWGNLLKIPVIPLN
HHCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCEEEEHHHHCCEEEEEEEECC
SDWFERASPRIILAAKQLCNALSQVN
CHHHHHCCCEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA