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The map label for this gene is pcnB [H]

Identifier: 16763574

GI number: 16763574

Start: 213874

End: 215292

Strand: Reverse

Name: pcnB [H]

Synonym: STM0184

Alternate gene names: 16763574

Gene position: 215292-213874 (Counterclockwise)

Preceding gene: 161353610

Following gene: 16763573

Centisome position: 4.43

GC content: 56.17

Gene sequence:

>1419_bases
ATGACACTACCGAGGTGCGCTATTTTTACCCGAGTCGCTAATTTTTGCCGTAAGGTGTTAAGCCGCGAGGAGAGCGAGGC
AGAACAAGCCGTCGCCCGTCCACATATGACGATTATCCCGCGTGAACAGCACGCTATCTCCCGCAAAGATATCAGTGAAA
ATGCCCTCAAGGTACTGTACAGGCTGAACAAAGCGGGCTATGAAGCCTACCTGGTCGGCGGCGGCGTCCGCGATCTCCTG
CTCGGCAAAAAGCCGAAGGATTTCGACGTGACTACCAACGCAACACCGGATCAGGTACGGAAATTATTCCGCAATTGCCG
TCTGGTGGGACGTCGTTTCCGCCTGGCTCACGTGATGTTTGGCCCGGAAATTATCGAAGTGGCAACGTTTCGTGGTCATC
ATGAAGGCAGTGAAAGCGACCGTACGACCTCCCAGCGTGGGCAAAACGGTATGCTGCTGCGCGACAACATCTTCGGCTCT
ATCGAAGAAGATGCCCAGCGCCGCGATTTCACCATCAACAGCCTTTACTACAGCGTGGCGGATTTTACCGTGCGCGATTA
CGTCGGCGGGATGCAGGATCTGCAAGAAGGCGTGATTCGCCTGATCGGCAATCCGGAAACGCGCTACCGCGAAGATCCGG
TTCGAATGCTGCGCGCCGTGCGTTTCGCTGCGAAGCTCAATATGCATATCAGCCCTGAAACGGCTGAGCCAATCCCGCGT
CTGGCAACCTTGCTAAACGACATTCCTCCCGCGCGCCTGTTCGAAGAGTCGCTGAAGCTGTTGCAGGCGGGGAACGGTTA
CGAAACCTATCAACAACTGCGGGAATACCACCTCTTCCAGCCGTTGTTTCCTACCATTACGCGTTATTTCACCGAAAACG
GCGACAGCGCAATGGAACGCATCATTGCGCAGGTGTTGAAGAATACGGATAACCGCATCCGTAACGAGATGCGCGTTAAT
CCGGCATTTTTGTTTGCCGCCATGTTCTGGTATCCGCTGCTGGAGATGGCGCAAAAAATCGCTCAGGAGAGCGGTCTGGC
CTATTACGACGCTTTCGCGCTGGCCATGAATGATGTGCTGGATGAGGCCTGCCGTTCACTGGCGATCCCGAAACGCCTTA
CCACGCTTACCCGTGATATTTGGCAGCTTCAGTTACGCATGTCGCGACGTCAGGGCAAACGCGCCTGGAAACTGATGGAA
CATCCCAAATTCCGCGCCGCGTTTGATTTACTGGAGCTGCGCGCTCAGGTGGAAAATAATACTGAACTGCAACGTCTGGC
GCAGTGGTGGGCCGAGTTTCAGGCTTCCGCGCCGCCGGAACAAAAAGGGATGCTCAACGAGCTGGACGACGATCCTGCTC
CACGCCGCCGTCGTTCACGTCCGCGCAAACGCGCGCCGCGCCGCGAGGGCACCGTATGA

Upstream 100 bases:

>100_bases
TGCTGCGGCGTTAATGATGCTTACAGCCCAGGTCGCGTTGCTCTCTTACGCCCGTCTGCCGTAATGCGAAAGACATAGAG
TAAGTTGATCTTATTAATGT

Downstream 100 bases:

>100_bases
CGATCGCGTATATCGCGCTTGGCAGTAATCTGGCCTCTCCGCTGGAGCAGGTCAATGCCGCCCTGAAGGCTATCGCCGAC
ATTCCCGATAGCCGTATCGT

Product: poly(A) polymerase I

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTLPRCAIFTRVANFCRKVLSREESEAEQAVARPHMTIIPREQHAISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLL
LGKKPKDFDVTTNATPDQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGSESDRTTSQRGQNGMLLRDNIFGS
IEEDAQRRDFTINSLYYSVADFTVRDYVGGMQDLQEGVIRLIGNPETRYREDPVRMLRAVRFAAKLNMHISPETAEPIPR
LATLLNDIPPARLFEESLKLLQAGNGYETYQQLREYHLFQPLFPTITRYFTENGDSAMERIIAQVLKNTDNRIRNEMRVN
PAFLFAAMFWYPLLEMAQKIAQESGLAYYDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLME
HPKFRAAFDLLELRAQVENNTELQRLAQWWAEFQASAPPEQKGMLNELDDDPAPRRRRSRPRKRAPRREGTV

Sequences:

>Translated_472_residues
MTLPRCAIFTRVANFCRKVLSREESEAEQAVARPHMTIIPREQHAISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLL
LGKKPKDFDVTTNATPDQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGSESDRTTSQRGQNGMLLRDNIFGS
IEEDAQRRDFTINSLYYSVADFTVRDYVGGMQDLQEGVIRLIGNPETRYREDPVRMLRAVRFAAKLNMHISPETAEPIPR
LATLLNDIPPARLFEESLKLLQAGNGYETYQQLREYHLFQPLFPTITRYFTENGDSAMERIIAQVLKNTDNRIRNEMRVN
PAFLFAAMFWYPLLEMAQKIAQESGLAYYDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLME
HPKFRAAFDLLELRAQVENNTELQRLAQWWAEFQASAPPEQKGMLNELDDDPAPRRRRSRPRKRAPRREGTV
>Mature_471_residues
TLPRCAIFTRVANFCRKVLSREESEAEQAVARPHMTIIPREQHAISRKDISENALKVLYRLNKAGYEAYLVGGGVRDLLL
GKKPKDFDVTTNATPDQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGSESDRTTSQRGQNGMLLRDNIFGSI
EEDAQRRDFTINSLYYSVADFTVRDYVGGMQDLQEGVIRLIGNPETRYREDPVRMLRAVRFAAKLNMHISPETAEPIPRL
ATLLNDIPPARLFEESLKLLQAGNGYETYQQLREYHLFQPLFPTITRYFTENGDSAMERIIAQVLKNTDNRIRNEMRVNP
AFLFAAMFWYPLLEMAQKIAQESGLAYYDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLMEH
PKFRAAFDLLELRAQVENNTELQRLAQWWAEFQASAPPEQKGMLNELDDDPAPRRRRSRPRKRAPRREGTV

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=276, Percent_Identity=30.4347826086957, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI87081691, Length=444, Percent_Identity=91.2162162162162, Blast_Score=844, Evalue=0.0,
Organism=Escherichia coli, GI1789436, Length=229, Percent_Identity=33.1877729257642, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71995920, Length=231, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321016, Length=287, Percent_Identity=26.1324041811847, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI281360112, Length=238, Percent_Identity=33.1932773109244, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357337, Length=238, Percent_Identity=33.1932773109244, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 54677; Mature: 54546

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPRCAIFTRVANFCRKVLSREESEAEQAVARPHMTIIPREQHAISRKDISENALKVLY
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHH
RLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTNATPDQVRKLFRNCRLVGRRFRLAHVMF
HHHHCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GPEIIEVATFRGHHEGSESDRTTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVA
CCHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEECCCCCCHHHHHHHHCCCHHHHHHHHH
DFTVRDYVGGMQDLQEGVIRLIGNPETRYREDPVRMLRAVRFAAKLNMHISPETAEPIPR
HHHHHHHHCCHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LATLLNDIPPARLFEESLKLLQAGNGYETYQQLREYHLFQPLFPTITRYFTENGDSAMER
HHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
IIAQVLKNTDNRIRNEMRVNPAFLFAAMFWYPLLEMAQKIAQESGLAYYDAFALAMNDVL
HHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
DEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLMEHPKFRAAFDLLELRAQVENN
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
TELQRLAQWWAEFQASAPPEQKGMLNELDDDPAPRRRRSRPRKRAPRREGTV
HHHHHHHHHHHHHCCCCCCHHCCCHHHCCCCCCCHHHHCCCHHCCCCCCCCC
>Mature Secondary Structure 
TLPRCAIFTRVANFCRKVLSREESEAEQAVARPHMTIIPREQHAISRKDISENALKVLY
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHH
RLNKAGYEAYLVGGGVRDLLLGKKPKDFDVTTNATPDQVRKLFRNCRLVGRRFRLAHVMF
HHHHCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GPEIIEVATFRGHHEGSESDRTTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVA
CCHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEECCCCCCHHHHHHHHCCCHHHHHHHHH
DFTVRDYVGGMQDLQEGVIRLIGNPETRYREDPVRMLRAVRFAAKLNMHISPETAEPIPR
HHHHHHHHCCHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LATLLNDIPPARLFEESLKLLQAGNGYETYQQLREYHLFQPLFPTITRYFTENGDSAMER
HHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
IIAQVLKNTDNRIRNEMRVNPAFLFAAMFWYPLLEMAQKIAQESGLAYYDAFALAMNDVL
HHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
DEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLMEHPKFRAAFDLLELRAQVENN
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
TELQRLAQWWAEFQASAPPEQKGMLNELDDDPAPRRRRSRPRKRAPRREGTV
HHHHHHHHHHHHHCCCCCCHHCCCHHHCCCCCCCHHHHCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]