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The map label for this gene is ilvI [H]

Identifier: 16763506

GI number: 16763506

Start: 135898

End: 137559

Strand: Direct

Name: ilvI [H]

Synonym: STM0116

Alternate gene names: 16763506

Gene position: 135898-137559 (Clockwise)

Preceding gene: 16763505

Following gene: 16763507

Centisome position: 2.8

GC content: 54.69

Gene sequence:

>1662_bases
GTGTTCGGCTATCCTGGAGGCGCAGTGCTTGATATTTATGATGCGCTACATACCGTTGGAGGGATCGATCATGTGCTGGT
GCGTCATGAGCAGGCCGCGGTGCATATGGCGGATGGCCTGGCGCGAGCCACTGGCGACGTTGGCGTGGTGCTGGTGACTT
CCGGACCGGGAGCGACCAATGCGATTACCGGGATTGCTACGGCCTATATGGATTCCATTCCGCTGGTGATTCTTTCCGGC
CAGGTCGCCACCTCATTGATTGGCTATGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCCCGACCGGTGGTCAAACA
TAGCTTCCTGGTCAAACAGACGGAAGATATTCCCCTGGTGCTTAAAAAGGCCTTCTGGCTGGCGGCAAGCGGACGTCCTG
GGCCGGTGGTCGTGGATTTGCCGAAAGATATTTTGAATCCGGCGAAAAAAATGCCGTATGCCTGGCCGGAGACGGTCAGT
ATGCGCTCTTACAATCCCACAACATCAGGACATAAAGGACAAATTAAGCGTGCCTTGCAAACGCTGGCGTCGGCAAAAAA
GCCGGTGGTCTACGTTGGGGGAGGGGCGATAAGCGCGGCCTGCTATGCGCCGTTGCGCCACATCATTGAAACCTTTAACC
TGCCGGTGGTCTCTTCATTAATGGGACTGGGCGCGTTTCCTGCTACTCATCGCCAGTCGTTAGGAATGCTGGGAATGCAT
GGCACTTATGAAGCCAATATGACCATGCATAACGCCGACGTGATTTTCGCTGTTGGCGTACGATTTGACGACCGCACGAC
GAATAATCTCGCTAAATATTGTCCGAACGCGACGGTATTACACATCGACATTGACCCGACGTCCATATCCAAAACGGTTA
ACGCTGATATCCCGGTGGTGGGCGATGCCCGCCTGGTACTGGAACAAATGCTGGAGCTACTGGCGCAGGATGCGCCGTCT
CAGCCGCAGGATGATATTCGCGACTGGTGGCAGCAGATAGAGAGCTGGCGCGCCCGTCAGTGCCTGAAATATGACGCGGA
AAGCGAAAGCATTAAACCCCAGGCGGTGATTGAAACCCTCTGGCGTCTGACGAAGGGCGATGCGTACGTGACGTCCGATG
TAGGACAACACCAGATGTTTGCCGCCCTCTATTACCCGTTCGATAAGCCACGTCGCTGGATTAATTCCGGCGGGCTCGGC
ACGATGGGTTTTGGCCTACCGGCTGCGCTGGGCGTTAAAATGGCCCTGCCGAAAGAAATGGTGGTCTGCGTGACCGGCGA
TGGCAGTATACAGATGAACATTCAGGAGCTGTCGACAGCCTTACAGTATGAACTGCCGGTACTGGTTTTGAACCTGAACA
ACCGTTATCTGGGGATGGTGAAACAGTGGCAGGATATGATCTATTCCGGTCGCCATTCTCAGTCTTATATGCAGTCGTTA
CCGGATTTTGTGCGCCTGGCGGAAGCATATGGCCATGTCGGGCTGCAGATTAACCGTCCGGATGAGCTGGAAAGTAAACT
CAGCGAAGCCCTTGAGCATGTGCGTAATAACCGACTGGTGTTCGTCGATGTCACCGTTGATGGCAGCGAGCATGTCTATC
CGATGCAGATTCGCGGGGGCGGGATGGACGAAATGTGGTTAAGCAAAACGGAGAGGACCTGA

Upstream 100 bases:

>100_bases
TCAAACCGTGATGACAGGTATAAAGTGGAGGCAAGCCGTGGAAATGTTGTCTGGAGCCGAGATGGTCGTCTGATCGCTTA
TCGATCAAGGCGTAAAGCAG

Downstream 100 bases:

>100_bases
TGATGCGCCGGATTTTATCGGTATTACTGGAAAACGAATCTGGGGCGTTATCGCGGGTTATCGGCCTCTTTTCGCAACGC
GGATATAATATTGAAAGCCT

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADGLARATGDVGVVLVTSGPGATNAITGIATAYMDSIPLVILSG
QVATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPLVLKKAFWLAASGRPGPVVVDLPKDILNPAKKMPYAWPETVS
MRSYNPTTSGHKGQIKRALQTLASAKKPVVYVGGGAISAACYAPLRHIIETFNLPVVSSLMGLGAFPATHRQSLGMLGMH
GTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVNADIPVVGDARLVLEQMLELLAQDAPS
QPQDDIRDWWQQIESWRARQCLKYDAESESIKPQAVIETLWRLTKGDAYVTSDVGQHQMFAALYYPFDKPRRWINSGGLG
TMGFGLPAALGVKMALPKEMVVCVTGDGSIQMNIQELSTALQYELPVLVLNLNNRYLGMVKQWQDMIYSGRHSQSYMQSL
PDFVRLAEAYGHVGLQINRPDELESKLSEALEHVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEMWLSKTERT

Sequences:

>Translated_553_residues
MFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADGLARATGDVGVVLVTSGPGATNAITGIATAYMDSIPLVILSG
QVATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPLVLKKAFWLAASGRPGPVVVDLPKDILNPAKKMPYAWPETVS
MRSYNPTTSGHKGQIKRALQTLASAKKPVVYVGGGAISAACYAPLRHIIETFNLPVVSSLMGLGAFPATHRQSLGMLGMH
GTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVNADIPVVGDARLVLEQMLELLAQDAPS
QPQDDIRDWWQQIESWRARQCLKYDAESESIKPQAVIETLWRLTKGDAYVTSDVGQHQMFAALYYPFDKPRRWINSGGLG
TMGFGLPAALGVKMALPKEMVVCVTGDGSIQMNIQELSTALQYELPVLVLNLNNRYLGMVKQWQDMIYSGRHSQSYMQSL
PDFVRLAEAYGHVGLQINRPDELESKLSEALEHVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEMWLSKTERT
>Mature_553_residues
MFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADGLARATGDVGVVLVTSGPGATNAITGIATAYMDSIPLVILSG
QVATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPLVLKKAFWLAASGRPGPVVVDLPKDILNPAKKMPYAWPETVS
MRSYNPTTSGHKGQIKRALQTLASAKKPVVYVGGGAISAACYAPLRHIIETFNLPVVSSLMGLGAFPATHRQSLGMLGMH
GTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVNADIPVVGDARLVLEQMLELLAQDAPS
QPQDDIRDWWQQIESWRARQCLKYDAESESIKPQAVIETLWRLTKGDAYVTSDVGQHQMFAALYYPFDKPRRWINSGGLG
TMGFGLPAALGVKMALPKEMVVCVTGDGSIQMNIQELSTALQYELPVLVLNLNNRYLGMVKQWQDMIYSGRHSQSYMQSL
PDFVRLAEAYGHVGLQINRPDELESKLSEALEHVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEMWLSKTERT

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=548, Percent_Identity=26.6423357664234, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI21361361, Length=570, Percent_Identity=26.4912280701754, Blast_Score=159, Evalue=5e-39,
Organism=Escherichia coli, GI87081685, Length=553, Percent_Identity=91.6817359855335, Blast_Score=1066, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=546, Percent_Identity=38.8278388278388, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1786717, Length=539, Percent_Identity=34.5083487940631, Blast_Score=308, Evalue=7e-85,
Organism=Escherichia coli, GI1787096, Length=545, Percent_Identity=28.4403669724771, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788716, Length=514, Percent_Identity=25.2918287937743, Blast_Score=162, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17531299, Length=543, Percent_Identity=26.8876611418048, Blast_Score=158, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17531301, Length=543, Percent_Identity=27.0718232044199, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17542570, Length=560, Percent_Identity=26.4285714285714, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6323755, Length=566, Percent_Identity=43.6395759717314, Blast_Score=441, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6320816, Length=471, Percent_Identity=25.0530785562633, Blast_Score=123, Evalue=6e-29,
Organism=Drosophila melanogaster, GI19922626, Length=487, Percent_Identity=28.7474332648871, Blast_Score=179, Evalue=6e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60625; Mature: 60625

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADGLARATGDVGVVLVTSGPGATN
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCH
AITGIATAYMDSIPLVILSGQVATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPLV
HHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHH
LKKAFWLAASGRPGPVVVDLPKDILNPAKKMPYAWPETVSMRSYNPTTSGHKGQIKRALQ
HHHHHHEEECCCCCCEEEECCHHHCCHHHHCCCCCCCCCEECCCCCCCCCCHHHHHHHHH
TLASAKKPVVYVGGGAISAACYAPLRHIIETFNLPVVSSLMGLGAFPATHRQSLGMLGMH
HHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHCCCCCCCC
GTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVNADIPVV
CEEECCEEEECCCEEEEEEEEECCCCHHHHHHHCCCCEEEEEECCCHHHCHHCCCCCCEE
GDARLVLEQMLELLAQDAPSQPQDDIRDWWQQIESWRARQCLKYDAESESIKPQAVIETL
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
WRLTKGDAYVTSDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPKEM
HHHHCCCEEEECCCCCHHEEHHEECCCHHHHHHHCCCCCCCCCCCCHHHHCCCEECCCEE
VVCVTGDGSIQMNIQELSTALQYELPVLVLNLNNRYLGMVKQWQDMIYSGRHSQSYMQSL
EEEEECCCCEEEEHHHHHHHHHEECCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHH
PDFVRLAEAYGHVGLQINRPDELESKLSEALEHVRNNRLVFVDVTVDGSEHVYPMQIRGG
HHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCC
GMDEMWLSKTERT
CCCHHHHHHCCCC
>Mature Secondary Structure
MFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADGLARATGDVGVVLVTSGPGATN
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCH
AITGIATAYMDSIPLVILSGQVATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPLV
HHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHH
LKKAFWLAASGRPGPVVVDLPKDILNPAKKMPYAWPETVSMRSYNPTTSGHKGQIKRALQ
HHHHHHEEECCCCCCEEEECCHHHCCHHHHCCCCCCCCCEECCCCCCCCCCHHHHHHHHH
TLASAKKPVVYVGGGAISAACYAPLRHIIETFNLPVVSSLMGLGAFPATHRQSLGMLGMH
HHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHCCCCCCCC
GTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVNADIPVV
CEEECCEEEECCCEEEEEEEEECCCCHHHHHHHCCCCEEEEEECCCHHHCHHCCCCCCEE
GDARLVLEQMLELLAQDAPSQPQDDIRDWWQQIESWRARQCLKYDAESESIKPQAVIETL
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
WRLTKGDAYVTSDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPKEM
HHHHCCCEEEECCCCCHHEEHHEECCCHHHHHHHCCCCCCCCCCCCHHHHCCCEECCCEE
VVCVTGDGSIQMNIQELSTALQYELPVLVLNLNNRYLGMVKQWQDMIYSGRHSQSYMQSL
EEEEECCCCEEEEHHHHHHHHHEECCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHH
PDFVRLAEAYGHVGLQINRPDELESKLSEALEHVRNNRLVFVDVTVDGSEHVYPMQIRGG
HHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCC
GMDEMWLSKTERT
CCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]